Application Of Honey Research Articles

Study of Brazilian Amazon Honeybees' (Melipona scutellaris and Apis mellifera) Properties in Healing Infected Skin Wounds in Wistar Rats

Introduction: In recent decades, studies have begun to analyze the therapeutic properties of honey, specifically in wound healing. The objective of this research was to investigate the tissue repair process of cutaneous wounds infected with Staphylococcus aureus in Wistar rats through the application of honey. Methods: The antibacterial analysis results were satisfactory, showing a reduction in bacteria in the Petri dishes. In the macroscopic analysis, there was a reduction in the wound area in all groups, but the groups treated with honey showed significantly better differences compared to the control group. Result: In the evaluation of the mean intensity of the inflammatory infiltrate, there was a reduction in the infiltrate in all groups, but no significant difference was observed. In the collagen evaluation, the groups that received treatment with Apis mellifera and Melipona scutellaris honey showed a greater deposition of collagen fiber bundles, with a significant difference compared to the control group. After 14 days there was total regeneration of the epidermis in most animals, with a greater amount of collagen fiber formation. Conclusion: Therefore, the work showed a novelty in terms of the screening of the use of a natural product for wound healing purposes giving prospects for substitution of traditional medicines by honeybee, being a sustainable alternative for human health.

Does the use of intra-alveolar honey after lower third molar extraction reduce postoperative inflammatory complications? A systematic review and meta-analysis.

Protocols are currently being studied in preventing postoperative complications after exodontia. This systematic review and meta-analysis aims to evaluate whether the application of intra-alveolar honey reduces inflammatory complications after the extraction of mandibular third molars (CRD 42.023.467.041). Searches were carried out in six electronic databases. Clinical trials comparing intra-alveolar honey administration with non-intervention or placebo after mandibular third molar extraction were selected to assess their impact on postoperative inflammatory parameters. The Cochrane ROB 2 tool was used to assess the bias risk in included studies, Stata software to conduct a meta-analysis for quantitative synthesis, and the GRADE system to evaluate the certainty of the evidence. This systematic review included 5 studies with 330 participants, and two studies were included in the meta-analysis. The results of the meta-analysis demonstrated that postoperative pain on the second day [MD: - 1.05; 95% CI - 2 to - 0.1] and fifth day [MD: - 0.97; 95% CI - 1.97 to - 0.03] was lower in the honey group compared to the control group. Total analgesic consumption [MD: - 4.77; 95% CI - 6.73 to - 2.81] was also lower in patients in the intervention group. The descriptive results indicated that honey appears to be beneficial in controlling edema, reducing trismus, and improving healing after extraction of third molars. Alveolar osteitis showed inconclusive results. The use of intra-alveolar honey after extraction of mandibular third molars seem to be associated with pain reduction. For the other outcomes, the results remain uncertain.

Effects of Diallium and Ziziphus honey in Second-Degree Burns on Wistar Rats

Background: Nowadays, the use of honeys in the treatment of burns and wounds has aroused growing interest due to its anti-inflammatory, antioxidant and healing properties. Aim: The aim of this study was to evaluate the effects of Diallium and Ziziphus honey in second degree burns on wistar rats. Methodology: A metal cylinder with a diameter of 3 cm was heated for five minutes. In order to cause experimental burns, the rats' shaved skin was lightly pressed with the cylinder for twenty seconds so as to induce second-degree burns. Results: The daily application of honey induces a healing dependent on typology and concentration. The healing rate is higher with DZ, with a score of 1.00 ± 0.63 vs 1.25 ± 0.43 for JUJ which induces almost complete tissue repair after 21 days of treatment. Conclusion: Findings from the research confirm the use of traditional honeys for burns and wound healing.

Wound Healing Activity of Two Honeys from the Southern Regions of Senegal (Casamance)

Aim: The aim of this study was to evaluate the healing activity of two honeys from Casamance (Senegal) in a second-degree experimental burn model in the Wistar strain rat. Methodology: Experimental burns were induced using a metal cylinder 3 cm in diameter and heated for 5 min. The cylinder was applied for 20 seconds, pressing lightly on the surface of the rats' shaved skin to induce second-degree burns. Results: The daily application of honey induces a healing dependent on concentration and typology. The healing rate is higher with AF, with a score of 0.60 ± 0.48 vs 1.00 ± 0.00 for MZ which induces almost complete tissue repair after 21 days of treatment. Conclusion: The results of this study demonstrate the use of traditional honey in wound healing and burns.

A Comparative Study between Honey and Alcohol as Topical Skin Disinfectant

Abstract Background Skin antisepsis is essential in every healthcare environment. Alcohol/ chlorhexidine use might disrupt skin microbiota and lead to antibiotic resistance. Objective To evaluate the efficacy of honey on reduction of skin microbes, on apparently healthy skin, and to compare the results with those of topical application of 70% isopropyl alcohol. Subjects and Methods This study was carried out on 70 children aged 2 to 12 years. Males represented 54.2% of the sample. Patients were recruited from the pediatric outpatient clinic, Children’s Hospital, Ain Shams University, Egypt, during the period from July 2022 to February 2023. A swab was taken from the skin of the dorsum of both hands for culture of skin microbes. Types and number of organisms were documented. Separately, honey and alcohol were applied alternatively on the dorsum of right and left hands. A skin swab was taken for microbiological examination before and 20 seconds after application of honey and alcohol. Results mean age of enrolled children is 7.34 (in years). Both honey and alcohol made significant reduction in the number of organisms on the dorsum of the hand (p = <0.001), and this effect was comparable between honey and alcohol with no statistically significant difference (p = 0.874). Before honey application, the median number of organisms is 65 colony forming unit (CFU), while 10 (CFU) is the median number of orgamisms after honey application. Before alcohol application, the median number of organisms is 80 (CFU), while 10 (CFU) is the median number of organisms after alcohol application. Percentage of reduction of organisms on dorsum of the hand after honey application is 62.5%, while 65% after alcohol application (p = 0.370). Staph coagulase negative, staph aureus, E-coli are most three common organisms were detected by swab. Conclusion Honey showed to be as effective as alcohol in reduction of skin microbes. We recommend further studies involving following up the rate of infection after minor surgical procedures to confirm the findings of our study.

An Interventional Study of Application of Manuka Honey in Post Mastoidectomy Surgery.

The aim of present study was to observe the effect of topical application of manuka honey in healing in the healing of postoperative mastoid cavity. A total of 100 patients diagnosed with chronic suppurative otitis media with cholesteatoma underwent canal wall down mastoidectomy, Manuka honey-soaked roller gauze pack was placed on Day 10 and was removed on day 17 and manuka honey was applied on day 17. Cavity was assessed on day 47 and day 90 on follow-up. Otoendoscopy was done on every visit to assess mastoid cavity. The primary outcome measure was the creation of a dry cavity as measured by a semi-quantitative scale as described by Merchant et al. Then Mastoid Cavity Healing Score was calculated on every visit and scoring was done at the end. On intragroup comparison it appeared that both the treatment methods exhibited improvised merchant grade score with the course of time (p-value = < 0.001). At 47th day and 90th day, the healing score in Group A was significantly higher than in Group B [p-value = < 0.001 and 0.001] respectively. We recommend Manuka honey as an adjunct to achieve a safe, dry ear in view of faster epithelization after mastoidectomy.

Exploring the antibiofilm effects on Escherichia coli biofilm associated with colon cancer and anticancer activities on HCT116 cell line of bee products

The association between dysbiotic microbiota biofilm and colon cancer has recently begun to attract attention. In the study, the apitherapeutic effects of bee products (honey, bee venom, royal jelly, pollen, perga and propolis) obtained from the endemic Yığılca ecotype of Apis mellifera anatoliaca were investigated. Antibiofilm activity were performed by microplate assay using crystal violet staining to measure adherent biofilm biomass of Escherichia coli capable of forming biofilms. Bee venom showed the highest inhibition effect (73.98%) at 50% concentration. Honey, perga and royal jelly reduced biofilm formation by >50% at all concentrations. The antiproliferation effect on the HCT116 colon cancer cell line was investigated with the water‑soluble tetrazolium salt‑1 assay. After 48 h of honey application at 50% concentration, cell proliferation decreased by 86.51%. The high cytotoxic effects of royal jelly and bee venom are also remarkable. Additionally, apoptotic pathway analysis was performed by ELISA using caspase 3, 8 and 9 enzyme-linked immunosorbent assay kits. All bee products induced a higher expression of caspase 9 compared with caspase 8. Natural products that upregulate caspase proteins are promising therapeutic targets for proliferative diseases.

Fabrication and characterization of alginate-zein core–shell microcapsules for controlled release of buckwheat honey

In this study, extrusion method was employed to fabricate alginate-zein core–shell microcapsules loaded with buckwheat honey by dropping alginate and buckwheat honey mixture solution into a 70.0 % zein ethanol solution(v/v) containing 5.0 % CaCl2 solution (wt%). The microcapsules were constructed by two parts: 1) the formation of hydrophilic beads through the crosslinking of alginate chains with Ca2+; 2) the introduction of alginate beads into the aqueous zein ethanol solution which decreased the ethanol concentration, prompting the precipitation of zein and the deposition of zein nanoparticles onto the surfaces of alginate beads. Comparing with the alginate beads, the prepared microcapsules not only possessed better water-holding capacity, but also achieved controlled release of buckwheat honey. Importantly, the microcapsules significantly retained the antioxidant activity of the buckwheat honey. Therefore, this innovative method for fabricating alginate-zein core–shell microcapsules can suggest a promising approach to broaden the application of buckwheat honey in the food field.

Synthesis, characterization, and application of honey stabilized inulin nanoparticles as colon targeting drug delivery carrier

Inulin (INU) is a versatile natural polysaccharide primarily derived from chicory roots. INU possesses the unique quality of evading digestion or fermentation in the early stages of the human digestive tract, instead reaching the lower colon directly. Exploiting on this distinctive attribute, INU finds application in the creation of targeted carrier systems for delivering drugs tailored to colon-related diseases. This study presents a novel method for synthesizing highly stable and non-aggregatory inulin nanoparticles (INU NPs) by ionotropic gelation method, using calcium chloride as crosslinker and natural honey as a stabilizing agent. Different formulation and process parameters were optimized for the synthesis of monodispersed INU NPs. These INU NPs efficiently encapsulated a hydrophilic drug irinotecan hydrochloride trihydrate (IHT) and drug loaded formulation (IINPs) demonstrated excellent colloidal and storage stabilities. Notably, these IINPs exhibited pH-dependent drug release, suggesting potential for colon-specific drug delivery. Anticancer activity of the NPs was found significantly higher in comparison to IHT through cytotoxicity and apoptosis studies against human colorectal carcinoma cells. Overall, this study revealed that the INU NPs synthesized by ionotropic gelation will be an efficient nanocarrier system for colon-targeted drug delivery due to their exceptional biocompatibility and stability in stomach and upper intestinal conditions.

Shoelace and honey technique: An innovative and inexpensive method of wound healing

Wound closure and wound infection have been a great enemy to orthopaedic surgeons since the beginning of time. Many wound closure technique have been described and controversy exists as a optimal method. Healing by secondary intension used to be commonly employed technique but less popular nowadays. Most wound closure require split skin thickness grafting which increases morbidity and scarring and additional wound at donor site .gradual mechanical dermal apposition technique that is shoelace technique have gained popularity and have shown to be effective in closure of wounds .Shoelace technique for wound closure is widely used in other medico-surgical specialties, this technique is unusual in orthopedics surgery, but the simplicity of the procedure, demanded us to follow to our cases.Honey is most ancient wound dressing biomaterial .Honey is known for its anti-oxidant, anti-bacterial and anti-inflammatory properties which were used for better and faster wound healing. Both the methods combined together make it a magic formula for the wound healing.We have 21 patients presenting to VDGMC, LATUR from a period from May 2020 to December 2023, with difficult primary wound closure were included in the study. The patients underwent the surgical fracture fixation (if any), followed by application of shoelace technique and honey dressing for the patient. An infant feeding tube and skin staples were used for the technique. Patients were followed till the complete wound healing occurred. The average time required for the wound healing using this technique was 3.2 weeks. Once the skin was approximated by dermatotraction, delayed primary suturing was done and regular dressing protocol was followed.Our study showed an excellent wound healing in 20 cases with duration of wound healing less den 3 weeks, which also waived of the need for an extra procedure of skin grafting. 1wound had continuous wound discharge which needed an extra debridement procedure longer duration for closure.The successful out-come observed in these cases, and at a very low cost, allows for its consideration as indicated for situations similar to that presented in this study, especially in patients with poor economic background.

First-Intention Incisional Wound Healing in Dogs and Cats: A Controlled Trial of Dermapliq and Manuka Honey.

This study aimed to compare incisional wound healing in cats and dogs after the topical application of Μanuka honey and a new medical device, Dermapliq. Comparisons were made between each treatment and control, between the two treatments, and between dogs and cats. Twelve cats and twelve dogs were included in this study, and the impact of the two substances was examined through cosmetic, clinical, ultrasonographical, and histological evaluation. The use of Dermapliq in first-intention wound healing achieved a significantly better cosmetic evaluation score and better total clinical score at days 20-41, compared to the control, in both dogs and cats. The ultrasonographically estimated wound area was smaller with Dermapliq compared to the control. Wounds treated with Dermapliq showed histologically less inflammation compared to the control. The use of Manuka honey did not show a significantly better cosmetic score compared to the control. Skin thickening was significantly higher after using Manuka honey compared to the control and so was the total clinical score. However, the median wound area, as was evaluated ultrasonographically, was significantly smaller when wounds were treated with Manuka honey, the difference being more apparent in dogs. Dermapliq was proven to be a better choice in achieving favorable wound healing than Manuka honey in dogs and cats in first-intention healing. In our study, cats had a statistically better cosmetic score and less skin thickening and scar width compared to dogs. Histologically, cats showed significantly less edema, higher inflammation and angiogenesis scores, and lower fibroblast and epidermis thickening scores when compared to dogs.

A QUASI-EXPERIMENTAL STUDY TO ASSESS THE EFFECTIVENESS OF TOPICAL APPLICATION OF HONEY ON ORAL MUCOSITIS AMONG HEAD AND NECK CANCER PATIENTS UNDERGOING RADIATION THERAPY IN TERTIARY CARE HOSPITAL OF AMRITSAR, PUNJAB.

Cancer is the leading cause of death around the world. Radiation therapy is made to kill growing cancer cells but certain normal cells get effected too, this damage to normal cell cause side effects of radiation therapy. The most debilitating side effect is radiation induced Oral Mucositis. This is major concern for attention, so there is need to provide conventional therapies to head and neck cancer patients to reduce the severity of oral mucositis. A Quasi-experimental study was conducted to assess the effectiveness of topical application of honey among 60 head and neck cancer patients undergoing radiation therapy at Sri Guru Ram Dass Institute of Medical Science and Research, Vallah, Amritsar, Punjab. Purposive sampling technique was used to collect data. The data wa

Prevention and Treatment of Oral Mucositis in Pediatric Patients: Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Oral mucositis (OM) is a common and serious side effect of cancer treatment. The incidence of chemotherapy-induced OM in pediatric patients can reach up to 91.5% and has a major impact on patients' quality of life. The aim of the study was to assess the efficacy of current interventions and agents for the management of OM in children undergoing chemo/radiotherapy or hematopoietic stem cell transplantation (HSCT). A systematic search of randomized controlled trials (RCTs) was conducted in the MEDLINE and Scopus databases from January 2000 until March 2023. Thirty-four randomized studies meeting the inclusion criteria were identified and five RCTs investigating the efficacy of Low Level Laser Therapy (LLLT) intervention or the agent honey were included in the meta-analysis. The meta-analysis of two RCTs indicated that topical application of honey on oral mucosa was effective in shortening the mean duration of hospital stay in children with severe OM (MD=-4.33, p=0.002). However, LLLT was not found to be effective for the prevention or treatment of OM grade ≥II (RR=0.99, p=0.99). Moreover, the therapeutic application of LLLT did not show significant benefit for lower risk of OM grade ≥II (RR=0.48, p=0.58). Various interventions and agents were examined in the present study for the management of OM. Honey could be a promising candidate for the treatment of OM in pediatric patients. Further high-quality RCTs are required to enhance our findings.

Honey therapies for dermatological disorders: more than just a sweet elixir.

Honey possesses antibacterial, anti-inflammatory, and healing properties that benefit wound healing and tissue regeneration. For centuries, honey has been utilized in traditional medicine as a binder or vehicle for creams and lotions and also for therapeutic purposes. The overuse of antibiotics and antimicrobial agents leading to drug resistance has emphasized the resurgence of honey's application in wound care. For many dermatological disorders, there is an interest in developing therapeutics with fewer side effects than traditional therapies and enhanced wound healing abilities to expedite tissue regeneration. This paper reviews the properties and components of honey that contribute to its wound-healing-based applications, the types of honey employed in medicine, and its dermatological applications. Based on the evidence from case reports, clinical trials, and invitro studies, honey has been characterized as a safe, cost-effective, and readily available treatment option for many skin conditions, including microbial infections, atopic dermatitis, psoriasis, necrotizing fasciitis, ulcers, as well as thermal and other types of wounds.

Exploring the Antibacterial, Anti-Biofilm, and Anti-Quorum Sensing Properties of Honey: A Comprehensive Review

Honey, a natural product derived from floral nectar and processed by bees, has long been recognized for its therapeutic properties. In recent years, extensive research has focused on exploring the antibacterial, anti-biofilm, and anti-quorum sensing activities of honey. This comprehensive review aims to summarize and analyze the current state of knowledge regarding the diverse antimicrobial properties of honey, with a particular emphasis on its effectiveness against bacterial infections, biofilm formation, and quorum sensing mechanisms. The review discusses the chemical composition of honey, including its various bioactive components, and examines their contributions to its antimicrobial effects. Furthermore, the mechanisms underlying honey’s antibacterial action, its ability to disrupt biofilm formation, and its interference with quorum sensing signaling pathways are elucidated. The review also highlights the potential applications of honey in clinical settings, wound management, and the development of novel therapeutic agents. By providing a thorough examination of the antibacterial, anti-biofilm, and anti-quorum sensing properties of honey, this review aims to contribute to a deeper understanding of its therapeutic potential and to inspire further research in this promising area.

Evaluating the effect of medical honey and stapler on skin graft fixation in burn wounds: A Randomized clinical Trial

Background One of the applications of honey is in wound healing. A stapler normally connects the skin graft to the underlying bed. Due to its high cost and lack of production in Iran, and causing physical damage to the tissue, an alternative method is needed. Objective This study aims to compare the effects of using medical honey and staplers for skin graft fixation in patients with burn wounds in the north of Iran. Methods In this clinical trial, 80 patients with deep second-degree and third-degree burns less than 40% of total body surface area were included and were randomly divided into two groups of honey (n=40) and stapler (n=40). Demographic information and the percentage of burns were extracted from patients’ medical files. The graft rejection, number of hospitalization days, grafted skin displacement, graft contracture, pain severity, existence of edema or hematoma, itching severity, and infection after grafting were investigated and recorded. Results Of 80 patients, 34 were male (42.5%) and 46 were female (57.5%). The mean age of the patients was 39.29±15.42 years. A significant difference was observed in the mean number of hospitalization days after grafting (P&lt;0.001), incidence of infection, hematoma, edema, and severity of pain and itching (P&lt;0.001), which were lower in the honey group. The graft contracture rate was lower in the stapler group (P&lt;0.001). Graft rejection was not observed in any groups. Conclusion The use of medical honey for skin graft fixation reduces the duration of hospitalization and adverse effects such as pain, itching, edema, hematoma, and infection compared to staplers. This method is as efficient as the routine treatment.

The use of honey in button battery ingestions: a systematic review.

Button battery (BB) ingestions may cause severe and possibly fatal complications, especially if the battery is located in the esophagus. The application of oral honey has recently been proposed by the National Capital Poison Center in the USA and in an ESPGHAN position paper in Europe, but clinical trials and experimental studies are limited. The goal of this systematic review was to analyze the evidence for this approach. A systematic review of clinical trials and experimental studies on the oral application of honey after BB ingestion in children was performed. Inclusion criteria according to the PICO format were patient age 0-18 years, ingestion of BB, oral administration of honey or other substances, all in vivo and in vitro studies, as well as reported complication rate, esophageal injury, and mortality. A manual search in the databases MEDLINE, Web of Science and Cochrane was performed to identify relevant search terms to form the following queries and to construct the extensive search. Furthermore, the search was extended by using snowballing on the reports reference lists. The review is registered at Research Registry. The identifying number is reviewregistry1581. We found four publications that investigated the effects of honey after button battery ingestion. Three of these presented experimental in vitro and in vivo results and one reported a clinical retrospective study of 8 patients. Follow up studies are required to further elucidate the effectiveness of the treatment with honey. The time intervals in which the use of honey is effective is not clear. Furthermore, a physiological model is needed for in vitro testing, preferably mimicking peristalsis and dynamic flow of the applied substances. However, since it is easy to apply and of minimal risk in patients over one year of age, honey should be considered a possible treatment option during the interval between presentation and endoscopic removal of the retained BB. https://www.researchregistry.com/browse-the-registry#registryofsystematicreviewsmeta-analyses/registryofsystematicreviewsmeta-analysesdetails/643e9df96750410027ee11b0/, identifier: reviewregistry1581.

The anti-allergic potential of stingless bee honey from different botanical sources via modulation of mast cell degranulation

BackgroundAllergy is an inflammatory disorder affecting around 20% of the global population. The adverse effects of current conventional treatments give rise to the increased popularity of using natural food products as complementary and alternative medicine against allergic diseases. Stingless bee honey, commonly known as Kelulut honey (KH) in Malaysia, has been used locally as a traditional remedy to relieve cough and asthma. This study evaluated the anti-allergic potential of KH collected from four different botanical sources on phorbol ester 12-myristate-3-acetate and calcium ionophore-activated human mast cells.MethodsThe present study examined the inhibitory effects of all collected honey on the release of selected inflammatory mediators, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-4, IL-6, IL-8, histamine, and β-hexosaminidase in an activated HMC. Besides that, all honey's total phenolic content (TPC) was also examined, followed by using liquid chromatography with tandem mass spectrometry (LC–MS/MS) to identify the phytochemicals in the honey. Further examination of the identified phytochemicals on their potential interaction with selected signaling molecules in an activated mast cell was conducted using computational methods.ResultsThe results indicated that there were significant inhibitory effects on all selected inflammatory mediators’ release by KH sourced from bamboo (BH) and rubber tree (RH) at 0.5% and 1%, but not KH sourced from mango (AH) and noni (EH). BH and RH were found to have higher TPC values and were rich in their phytochemical profiles based on the LC–MS/MS results. Computational studies were employed to determine the possible molecular target of KH through molecular docking using HADDOCK and PRODIGY web servers.ConclusionsIn short, the results indicated that KH possesses anti-allergic effects towards an activated HMC, possibly by targeting downstream MAPKs. However, their anti-allergic effects may vary according to their botanical sources. Nevertheless, the present study has provided insight into the potential application of stingless bee honey as a complementary and alternative medicine to treat various allergic diseases.

The effects of bee species and vegetation on the antioxidant properties of honeys produced by Afrotropical stingless bees (Hymenoptera, Apidae, Meliponini)

Stingless bee honey is widely utilized as a traditional medicine in tropical and subtropical regions. The rich diversity of stingless bees and floral resources in the African tropics presents an opportunity for the production of honeys with diverse therapeutic properties. This study investigated the influence of bee species and vegetation on the antioxidant properties of stingless bee honey. Honey samples were collected from six species of Afrotropical stingless bees in six different vegetation zones of Tanzania, and tested for phytochemical composition and antioxidant activity. Our findings demonstrate that the antioxidant properties of stingless bee honey were influenced by both bee species and vegetation, with the former exhibiting a stronger effect. Honeys produced by different stingless bee species varied significantly in all of the assayed parameters, whereas honey samples from different vegetation zones differed in 80% of the parameters. Further, there was a clear separation of honey samples based on bee species on the non-metric multidimensional scaling (NMDS) and principal component analysis (PCA) plots which suggests that the antioxidant properties can be used as markers for discriminating honeys produced by different bee species. The studied stingless bee species across different vegetation zones produced honeys with remarkable phenolic content (46.80–365.17 mg GAE/100 g), flavonoid content (27.49–210.83 mg QE/100 g), vitamin C (7.42–60.50 mg/100 g), radical scavenging activity (32.75–92.50%) and ferric reducing antioxidant power (68.11–221.23 μmol Fe (II)/100 g). Stingless bee honey presents a promising niche for pharmaceutical research and innovation, particularly for Dactylurina schmidti honey which demonstrated exceptional levels of phytochemicals and antioxidant activity. We encourage future studies to investigate the biologically active components in honeys produced by Afrotropical stingless bees, paving the way for potential applications of stingless bee honey in clinical therapy.

Application of beekeeping products in the technology of raw smoked sausages

The safety of fermented sausage products is due to the elimination of a number of risks of biological origin, mainly of a microbiological nature. One of the most promising directions for the development of barrier technologies is the use of starter cultures and the biologically active substances produced by them in the production of fermented meat products. The research aims to study the influence of starter cultures and honey on a complex of physico-chemical, structuralmechanical, organoleptic indicators and nutritional value of raw smoked sausages. The article presents changes in the physical and chemical parameters of raw smoked sausages when using starter cultures in combination with different types of honey. When performing the work, 4 types of honey were used: from sunflower, phacelia, honeydew honey and from medicinal herbs. At the first stage of research, the proportion of honey application and the type of starter crops were selected. The next stage of the study was to determine the impact of the starter cultures included in the product BIOSTART PLUS 50 (lactic acid bacteria Lactobacillus Sake and yeasts Debaryomyces Hansenii) and honey on moisture loss during fermentation of raw smoked sausages, physicochemical parameters of finished products. Determination of moisture content in samples was carried out by drying in a drying cabinet at a temperature of 103±2°C. Conducted studies prove that the use of the product BIOSTART PLUS 50 (lactic acid bacteria Lactobacillus Sakei and yeasts Debaryomyces Hansenii) and honey allows shortening the ripening and drying technological process. A comparative analysis of manufactured raw-smoked sausages in terms of nutritional value makes it possible to state that manufactured raw-smoked sausages with the addition of honey meet the requirements of the standard. The results of the set of studies carried out in this work showed that the use of a composition of lactic acid bacteria and yeast with the use of honey as a source of carbohydrates will allow intensifying the technological process and improve the quality of finished products