Application Of Dental Stem Cells Research Articles

Significance of Dental Stem Cells in Dentistry and Stem Cell Banking

Dentists play a crucial role in collecting stem cells for dental treatments and the potential treatment of medical diseases. Considering dental stem cells (DSCs) in terms of their increasing potential in medicine, adequate knowledge and positive attitudes of dentists toward DSCs and their use in regenerative therapies are extremely important. The immense potential of DSCs in regenerative medicine applications for various treatments suggests their significant role in both dental treatments and innovative approaches to treating various diseases. “Dental banking” emerged as a result of DSCs’ use in medical applications and their numerous clinical advantages across regenerative medical domains. DSC banking is a service that makes it possible to store and utilize DSCs for potential medical applications. However, further clinical research is needed to advance the applications of DSCs in treatment. This review aims to summarize the current literature on dental stem cell banking applications and usage areas of DSCs in dentistry.

Originating at Teeth to Tranquility: The Application of Dental Stem Cells in the Treatment of Emotional Disturbance

Abstract not available
 Bangladesh Journal of Medical Science Vol. 23 No. 02 April’24 Page : 290-292

Advances in combined application of dental stem cells and small-molecule drugs in regenerative medicine.

Teeth are a kind of masticatory organs of special histological origin, unique to vertebrates, playing an important role in chewing, esthetics, and auxiliary pronunciation. In the past decades, with the development of tissue engineering and regenerative medicine, the studies of mesenchymal stem cells (MSCs) gradually attracted the interest of researchers. Accordingly, several types of MSCs have been successively isolated in teeth or teeth-related tissues, including dental pulp stem cells, periodontal ligament stem cells, stem cells from human exfoliated deciduous teeth, dental follicle stem cells, stem cells from apical papilla and gingival mesenchymal stem cells. These dental stem cells (DSCs) are easily accessible, possess excellent stem cell characteristics, such as high proliferation rates and profound immunomodulatory properties. Small-molecule drugs are widely used and show great advantages in clinical practice. As research progressed, small-molecule drugs are found to have various complex effects on the characteristics of DSCs, especially the enhancement of biological characteristics of DSCs, which has gradually become a hot issue in the field of DSCs research. This review summarizes the background, current status, existing problems, future research directions, and prospects of the combination of DSCs with three common small-molecule drugs: aspirin, metformin, and berberine.

Potential application of dental stem cells in regenerative reconstruction of oral and maxillofacial tissues: a narrative review.

Background and Objective:Oral and maxillofacial (OMF) defects caused by congenital conditions, injuries, ablative surgery for benign and malignant head & neck tumor, can often lead to OMF deformities and malfunctions in speech, mastication/chewing, and swallowing as well as have deleterious psychological effects and socioeconomic burdens to patients. Due to the unique complex 3D geometry of the head and neck region, reconstruction and rehabilitation of OMF defects remain a major challenge for OMF surgeons.The purpose of this narrative review is to update the information on the biological properties and functions of mesenchymal stem cells derived from various dental tissues (dental-MSCs) and their potential application in tissue engineering (TE) and regenerative reconstruction of OMF tissues.Methods:A data-based search was performed by using PubMed database whereby articles published between 2000 and 2021 in English were included in the search with the following key words: dental stem cells, OMF reconstruction, OMF TE and regeneration.Key Content and Findings:Currently, the advancement in stem cell biology, biomaterial science, and TE technology has demonstrated the significant potential application of stem cell-based therapy in regenerative reconstruction and rehabilitation of OMF defects. However, no stem cell-based product or device has been translated into clinical application to replace microsurgical free tissue transfer, the current mainstay of care in the reconstruction of OMF defects.Conclusions:Currently, microsurgical free tissue transfer remains the gold standard mainstay of care for the reconstruction of OMF defects due to their abundant blood supply and flexibility for transplantation. However, several major challenges, such as the limited availability, the requirement of a second surgery, donor site morbidity, and the risk of free flap failure, have promoted the development of novel approaches. Due to the advancement in stem cell biology, biomaterial science, and TE technology, stem cell-based regenerative therapy is emerging as a promising therapeutic approach for a variety of diseases, including regenerative reconstruction and rehabilitation of OMF defects. In this narrative review, we update on the characteristics and biological functions of mesenchymal stem cells derived from various dental tissues (dental-MSCs) and their released cell-free products, extracellular vesicles (EVs). We also highlighted their potential application in TE and regenerative reconstruction of OMF defects in animal models and clinical studies and the potential challenges in this field.

Knowledge, Attitude and Practice Regarding Dental Stem Cells and Tooth Banking among Dental Professionals: A Questionnaire-based Cross-sectional Study

Introduction: Stem cell therapy is new revolutionary technique which can repair many defects of human body. There are two sources of stem cells, one is embryonic and postnatal. Postnatal sites are the ones from which procuring stem cells is easiest. Dental stem cells from exfoliated deciduous teeth are also obtained from these sources. Dental health professionals must have understanding about dental stem cells for its application. Hence, there is need to evaluate knowledge, attitude and practice of dental professionals regarding dental stem cells. Aim: To evaluate knowledge, attitude and practice regarding dental stem cells and tooth banking among dental professionals of Gujarat. Materials and Methods: The study was a questionnaire based cross-sectional study conducted in the Department of Paediatric and Preventive Dentistry, KM Shah Dental College and Hospital, Sumandeep Vidyapeeth, Vadodara, Gujarat, India from December 2020 to March 2021. Total of dental health professionals participated including undergraduate (interns) and postgraduate students, dental practitioners and faculty members of dental colleges. Participants received the questionnaire in the form of a google form through email and whatsApp. The data was analysed using percentages of total and Chi-square test for statistical analysis. Results: Out of 711 participants, 410 were students, 44 faculties, 207 practitioners and 50 faculties and practitioner both. Among the faculty, knowledge on types of dental stem cells was significantly associated with the years of experience (p-value <0.001). Among the students, first year postgraduate students and interns had significantly less knowledge regarding various applications of dental stem cells (p-value <0.001). Among them, 52.9% participants were aware of stem cell banks in India and 55.5% were aware of names of stem cell banks. Participants suggested that seminars, journals, academic curriculum, conferences or Continued Dental Education (CDE), and advertisement and public notice all should be used to increase awareness of dental stem cells among dental professionals. Conclusion: There was adequate awareness among dental health professionals regarding dental stem cells and their applications but were unaware about tooth banking and its procedure. The study also found that there was lack of knowledge regarding steps of preservation of dental stem cells and their ethical concerns. Therefore, the topic of dental stem cells should be included in dental curriculum.

Dental stem cells in intraoral regenerative therapy: a systematic review

Objective: 1) To critically review the published literature on applications of dental stem cells in the regenerationof intraoral tissues. 2) To provide an evidence-based level on research regarding application of dental stem cellsin intraoral tissues regeneration. Methodology: This systematic review is conducted as per the JBI guidelinesand reported as per the PRISMA. An initial literature search of papers published between 2004 and 2018 yielded421 manuscripts. Nineteen studies satisfied the inclusion / exclusion criteria and were included for qualitativesynthesis. Studies were categorized as animal (11) and human (8) trials. Five independent reviewers criticallyassessed the included studies. Risk of bias was assessed using SYstematic Review Centre for Laboratory animalExperimentation (SYRCLE) bias risk tool, robins-I tool for non-randomised clinical trial and Cochrane Collaboration’sTool for randomised clinical trial. Evidence levels were assessed based on JBI Criteria. Results: Animal trialsmainly focused on periodontal regeneration. A high or unclear Risk of bias was more commonly found amongstanimal studies. Laboratory, clinical and radiographic evaluation were used to assess the outcome. A total ofEight Human studies were conducted on a total samples size of 153 upon a wide age ranging from seven yearsto 60 years. Nearly 70% of the human studies used DPSC for regenerating alveolar bone defects. Conclusion:Appropriate well designed double-blind randomized clinical trials of longer duration are yet to be performed.Evidence for the included studies were 1C and 1D as per the JBI Criteria. Stem cell therapy demonstratedpromising results in Periodontal tissue and alveolar bone regeneration. However, the number of studies to claimsuch a benefit are very limited. KEYWORDSDental stem cell (DSCs); Stem cells from human exfoliated deciduous teeth (SHED); Dental pulp stem cells(DPSC); Intraoral application; Human study; Animal study.

Application of dental stem cells in three-dimensional tissue regeneration.

Dental stem cells can differentiate into different types of cells. Dental pulp stem cells, stem cells from human exfoliated deciduous teeth, periodontal ligament stem cells, stem cells from apical papilla, and dental follicle progenitor cells are five different types of dental stem cells that have been identified during different stages of tooth development. The availability of dental stem cells from discarded or removed teeth makes them promising candidates for tissue engineering. In recent years, three-dimensional (3D) tissue scaffolds have been used to reconstruct and restore different anatomical defects. With rapid advances in 3D tissue engineering, dental stem cells have been used in the regeneration of 3D engineered tissue. This review presents an overview of different types of dental stem cells used in 3D tissue regeneration, which are currently the most common type of stem cells used to treat human tissue conditions.

Applications Of Dental Stem Cells For Regenerative Medicine In Oral Surgical Procedures

Applications Of Dental Stem Cells For Regenerative Medicine In Oral Surgical Procedures

Knowledge, Awareness, Attitude, and Opinion about Application of Dental Stem Cells: A Dental School-Based Questionnaire Study

Abstract Objective The aim of the study is to assess the knowledge, awareness, attitude, and opinion concerning dental stem cells among faculty, postgraduates, and interns in a dental school. Materials and Methods A questionnaire consisting of 12 questions was circulated among the 150 participants (50 faculty, 50 postgraduate students, and 50 interns). A questionnaire was prepared that could assess the basic knowledge on stem cells (dental) based on similar previous studies. The questionnaire data were entered by the investigator in Microsoft Excel 2010 on a corresponding day. Using Predictive Analytics Tools (Version 18), the analysis was done. Probability ratios by Pearson were used to analyze the application of knowledge, perception regarding stem cells. Results The survey participants consisted of ~73% of females and 27% of males. Most of the participants have come across the term stem cells and thought that only pulp stem cells signified the only source of dental stem cells. The majority of the participants pointed out the anticipated high cost to be the biggest obstacle to a patient accepting regenerative dental treatment by stem cells. Conclusion This study reveals a good level of knowledge among dental professionals, and it also showed the need to develop more awareness about the advancements in applications and banking.

Clinical applications of dental stem cells in modern regenerative medicine: A systematic review with updates.

The use of dental stem cells (DSCs) has emerged as a promising new approach for therapeutic purposes to treat dental and non-dental diseases. Thus, the aim of this systematic review was to compile all current information on the role and clinical applications of DSCs in modern regenerative medical therapy. PubMed and Google Scholar electronic databases were used to search the literature for relevant studies after applying specific inclusion and exclusion criteria. The search included articles that were published from 2009 to 2019. Several keywords were combined for the search: (1) "Clinical applications", (2) "Dental Stem Cell", and (3) "Medicine". Only the 17 studies that fulfilled both the inclusion and exclusion criteria were included in this systematic review. These studies investigated different aspects of DSCs, including cell types, clinical applications, and updates of their use in regenerative medicine. All 17 studies favored the use of different DSCS in regenerative medicine to treat diseases, such as bone defects, neural and skin injuries, Parkinson's disease, ischemia, and others. None of the studies were conducted on humans. This systematic review demonstrated the growing body of evidence supporting the role of DSCs in the field of modern generative medicine. The noninvasive methods of isolating these cells compared to those for isolating non-DSCs make them promising potential sources for the treatment of chronic and devastating diseases. However, more studies are needed to develop the proper guidelines for cases in which DSCs could be considered an accurate and reliable tool for modern regenerative medicine in clinical trials.

DENTAL STEM CELLS (CONCEPTS AND APPLICATIONS)

Stem cells constitute the source of differentiated cells for the generation of tissues during development, as well as for regeneration of tissues that are diseased or injured postnatally. The stem cell research has grown exponentially to improve the life of patients with conditions that span from Alzheimer’s disease, cardiac ischemia to bone or tooth loss. In dentistry, stem cell biology and tissue engineering are of great interest since they may provide an innovative for generation of clinical material and/or tissue regeneration. Mesenchymal stem cells were demonstrated in dental tissues, including dental pulp, periodontal ligament, dental papilla, and dental follicle. These stem cells can be isolated and grown under defined tissue culture conditions and are potential cells for use in tissue engineering including dental tissue, nerves and bone regeneration under appropriate conditions. This review was performed to study the concepts of stem cells and the most recent application of dental stem cells.

Advances in the research of application of dental stem cells in neural tissue engineering

Dental stem cell is a kind of stem cell isolated from dental hard tissue or periodontal tissue, including dental pulp stem cell, stem cell from human exfoliated deciduous teeth, stem cell from root apical papilla, periodontal ligament stem cell, dental follicle progenitor cell, and so on. As seed cell, dental stem cell provides safe and efficient cell source for nerve tissue engineering research. The review aims to introduce the characteristics of these dental stem cells in promoting the regeneration and preparation of nerve and the clinical application.

Progress in storage and clinical application of dental stem cells

Progress in storage and clinical application of dental stem cells

Dental Stem Cells in Bone Tissue Engineering: Current Overview and Challenges.

The treatment of bone that is impaired due to disease, trauma or tumor resection creates a challenge for both clinicians and researchers. Critical size bone defects are conventionally treated with autografts which are associated with risks such as donor site morbidity and limitations like donor shortage. Bone tissue engineering has become a promising area for the management of critical size bone defects by the employment of biocompatible materials and the discovery of novel stem cell sources. Mesenchymal stem cells (MSCs) can be isolated with ease from various dental tissues including dental pulp stem cells, stem cells from apical papilla, dental follicle stem cells, stem cells from human exfoliated deciduous teeth, periodontal ligament stem cells, gingival stem cells and tooth germ derived stem cells. Outcomes of dental MSC mediated bone tissue engineering is explored in various in vivo and in vitro preclinical studies. However, there are still obscurities regarding the mechanisms underlying in MSC mediated bone regeneration and challenges in applications of dental stem cells. In this review, we summarized dental stem cell sources and their characterizations, along with currently used biomaterials for cell delivery and future perspectives for dental MSCs in the field of bone tissue engineering. Further efforts are necessary before moving to clinical trials for future applications.

Dental Tissue-Derived Stem Cells as a Candidate for Neural Regeneration

In recent years, stem cell therapy tried to improve the life of patients that suffer from neurodegenerative disease, like Alzheimer's disease. Although teeth are non-essential for life, but the dental tissues are an important source of mesenchymal stem cells that are suitable for neural regeneration. The studies showed that dental stem cells (DSCs) have the potential to differentiate into several cell types that among the most important is neural progenitor. In this review article, discusses the types of dental stem cells and then focused on application of dental stem cells on neural regeneration.

Stem cells in dentistry: A study regarding awareness of stem cells among dental professionals.

Dental stem cell, a type of adult stem cell, exhibits multipotent differentiation capacity and is drawing worldwide attention because of its numerous applications. The advances in applications of dental stem cells seem to be unsurpassed in the near future, for which specialized skills and knowledge in this arena are of prime significance. Hence, there is a need to acquire more knowledge about dental stem cells to obtain maximum benefits from it in the coming years. Dental stem cells in India are still at the budding stage, and there seems to be limited awareness regarding dental stem cells. This study aimed to assess the awareness of stem cells among the dental professionals. The present study was a questionnaire-based study of dental professionals (MDS, BDS, postgraduates, and interns) of three different institutions. Results showed that 95.2% of dental professionals are aware of the terminology dental stem cells and 53.9% of them are aware of various applications of dental stem cells. Chi-square test showed a significant correlation between the sources of information, source of dental stem cells, and clinical applications in relation to the academic qualification of the dental professionals. This study revealed a good level of awareness among the dental professionals, and it also showed the need to spread more knowledge about the advances in applications, storage, banking, and guidelines related to dental stem cells.

Cryopreservation and Banking of Dental Stem Cells.

Over the past decade, dental tissues have become an attractive source of mesenchymal stem cells (MSCs). Dental stem cells (DSCs) are not only able to differentiate into adipogenic, chondrogenic and osteogenic lineanges, but an increasing amount of research also pointed out their potential applicability in numerous clinical disorders, such as myocardial infarction, neurodegenerative diseases and diabetes. Together with their multilineage differentiation capacity, their easy availability from extracted third molars makes these stem cells a suitable alternative for bone marrow-derived MSCs. More importantly, DSCs appear to retain their stem cell properties following cryopreservation, a key aspect in their long-term preservation and upscale production. However, the vast number of different cryopreservation protocols makes it difficult to draw definite conclusions regarding the behavior of these stem cells. The routine application and banking of DSCs is also associated with some other pitfalls, such as interdonor variability, cell culture-induced changes and the use of animal-derived culture medium additives. Only thorough assessment of these challenges and the implementation of standardized, GMP procedures will successfully lead to better treatment options for patients who no longer benefit from current stem cell therapies.

Application of dental stem cells in regenerative medicine

Recent discoveries in the field of stem cells place dental professionals at the forefront of helping their patients with potentially life-saving therapies derived from a patient's own stem cells obtained from deciduous or permanent teeth. Stem cells have opened doors of great hope in that these cells produced in large quantities through cell cultures could be injected into failing tissues and organs, which would replace these damaged cells with fresh ones. The emerging field of personalized medicine has focused on the ability of stem cells to grow and regenerate tissues using a patient's own stem cells for biologically compatible therapies and individually tailored treatments. This review discusses the information available on the different types of dental stem cells (DSCs) and their potential role in both dental and medical regenerative therapies.

Redefining the potential applications of dental stem cells: An asset for future

Recent exciting discoveries isolated dental stem cells from the pulp of the primary and permanent teeth, from the periodontal ligament, and from associated healthy tissues. Dental pulp stem cells (DPSCs) represent a kind of adult cell colony which has the potent capacity of self-renewing and multilineage differentiation. Stem cell-based tooth engineering is deemed as a promising approach to the making of a biological tooth (bio-tooth) or engineering of functional tooth structures. Dental professionals have the opportunity to make their patients aware of these new sources of stem cells that can be stored for future use as new therapies are developed for a range of diseases and injuries. The aim of this article is to review and understand how dental stem cells are being used for regeneration of oral and conversely nonoral tissues. A brief review on banking is also done for storing of these valuable stem cells for future use.

Potential feasibility of dental stem cells for regenerative therapies: stem cell transplantation and whole-tooth engineering

Multipotent mesenchymal stem cells from bone marrow are expected to be a somatic stem cell source for the development of new cell-based therapy in regenerative medicine. However, dental clinicians are unlikely to carry out autologous cell/tissue collection from patients (i.e., marrow aspiration) as a routine procedure in their clinics; hence, the utilization of bone marrow stem cells seems impractical in the dental field. Dental tissues harvested from extracted human teeth are well known to contain highly proliferative and multipotent stem cell compartments and are considered to be an alternative autologous cell source in cell-based medicine. This article provides a short overview of the ongoing studies for the potential application of dental stem cells and suggests the utilization of 2 concepts in future regenerative medicine: (1) dental stem cell-based therapy for hepatic and other systemic diseases and (2) tooth replacement therapy using the bioengineered human whole tooth, called the "test-tube dental implant." Regenerative therapies will bring new insights and benefits to the fields of clinical medicine and dentistry.