Application Of CB Research Articles

Functionalization of semi-conductive interfaces with cucurbit[n]urils

Abstract: Cucurbiturils are a class of macrocyclic compounds with highly polar carbonyl portals and a hydrophobic cavity. They are usually utilized as efficient host molecules in supramolecular chemistry applications due to their high binding affinity for positively charged or cationic compounds. This review investigates the application of CB[n] immobilization at semi-conductive interfaces to produce electrochemical sensors. Critical to the production of thin film electrode preparations is the solubility of the CB[n] to produce homogeneous thin films when deposited. The solubility of CB[n] molecules in organic and inorganic solvents is limited; however, CB[7] has been applied successfully in the production of a wide range of electrochemical sensors. Furthermore, we introduce simple drop-casting of efficiently solubilized CB[7] as a simple yet effective method for producing CB[7] modified electrochemical sensors for the sensitive reporting of dopamine in aqueous solutions in the concentration range of 3.33×10-9 to 1.16×10-8 M with a sensitivity of 0.315 µA/M, (n=3).

Structure-guided engineering of aptamers to enhanced structural stability and application performance in alleviating β-lactoglobulin allergenicity

Aptamers are synthetic single-strand oligonucleotide-based ligands with great application potential due to their intriguing properties of specific and high affinity target binding and inhibition. However, the excessive flexibility of aptamer conformation will lead to instability and diversity of the folded structure. It remains a great challenge to keep high binding performance in real-world applications. Herein, we rational engineered a structurally enhanced aptamer (Cb-#T4M8) through hairpin structural enhancement guided by structural information combined with cyclization strategies, that possesses excellent binding affinity (5.1 ± 1.2 nM) against β-lactoglobulin (β-Lg), as well as highest stability. Notably, molecular simulation and competitive experimental analysis revealed that Cb-#T4M8 shares the same binding epitopes on β-Lg with IgE. Using Cb-#T4M8 as an inhibitor, Cb-#T4M8 was successfully demonstrated to interfere with IgE binding to β-Lg in vitro experiments. Furthermore, The application of Cb-#T4M8 in allergy models of cells and mice significantly reduced the allergic response induced by β-Lg. Overall, our study not only provided a reference for the engineering of aptamers to obtain a good application in the real world but also proposed a new direction for developing treatment against food allergy.

Impacts of NPK consortia biofertilizer and mineral fertilizer on growth and yield of two maize (Zea mays L.) hybrids in Rajasthan-India

Inoculation of important microbial strains in a modern intensive crop production is a critical step for the improvement of hybrid crop production. This study evaluated the impact of NPK consortia biofertilizer (NPK CB) and mineral fertilizer on growth and yield of two maize (Zea mays L.) hybrids at Mewar University research farm, India. The research was conducted during 2020/2021 Kharif cultivation season. The split-plot design was adopted in three replications, each consisting of six treatments combinations; (T1 = control, T2 = 50% Recommended Dose of Fertilizers (RDF), T3 = 100% RDF, T4 = NPK CB, T5 = 50% RDF + NPK CB and T6 = 100% RDF + NPK CB) and two maize hybrids (i.e., N.K-30 and N.K-30 plus). The result obtained revealed that the growth attributes, and yield attributes increased due to the combined application of NPK CB and mineral fertilizers. But there is no significant difference (p>0.05) observed between the studied hybrids, except for the 1000 kernels weight. Although the highest grain yield (1987.39 kg ha-1) obtained from the application of T6 (100%RDF + NPK CB) was comparable with the grain yield (1957.64 kg ha-1) obtained from the application of T3 (100% RDF). However, inoculation with NPK CB had superior effects on growth and yield attributes over the sole application of mineral fertilizers below the RDF. Hence, NPK CB could be a potent fertilizer input for hybrids maize production while reducing the level of chemical fertilizers below the RDF.

Cucurbituril‐Based Supramolecular Polymers for Biomedical Applications

AbstractSupramolecular polymers (SPs) have attracted broad interest because of their intriguing features and functions. Host–guest interactions often impart tunable physicochemical properties, reversible hierarchical organization, and stimuli‐responsiveness to SPs for diverse biomedical applications. Characterized by strong but dynamic interactions with guest molecules, cucurbit[n]uril (CB[n]) has shown great potential as an important building block of various functional polymers for biomedical applications. In this Minireview, we summarize the most recent examples regarding the design, fabrication, and biomedical applications of CB[n]‐based supramolecular polymers (CSPs), which are categorized as noncovalent and covalent CSPs according to the interactions between the CB[n] and polymer backbones. The design principles of CSPs and their unique advantages for biomedical applications, as well as the developmental trends and future perspectives of this cross‐disciplinary area are also discussed.

Cucurbituril-Based Supramolecular Polymers for Biomedical Applications.

Supramolecular polymers (SPs) have attracted broad interest because of their intriguing features and functions. Host-guest interactions often impart tunable physicochemical properties, reversible hierarchical organization, and stimuli-responsiveness to SPs for diverse biomedical applications. Characterized by strong but dynamic interactions with guest molecules, cucurbit[n]uril (CB[n]) has shown great potential as an important building block of various functional polymers for biomedical applications. In this Minireview, we summarize the most recent examples regarding the design, fabrication, and biomedical applications of CB[n]-based supramolecular polymers (CSPs), which are categorized as noncovalent and covalent CSPs according to the interactions between the CB[n] and polymer backbones. The design principles of CSPs and their unique advantages for biomedical applications, as well as the developmental trends and future perspectives of this cross-disciplinary area are also discussed.

Effect of modified corn residue biochar on chemical fractions and bioavailability of cadmium in contaminated soil

ABSTRACT In order to study the effects of FeCl2 modified corn residue biochar (Fe-MCB) on the bioavailability and chemical fractions of cadmium (Cd) in a contaminated calcareous soil, an experiment was conducted as a factorial in completely randomised design with three replications. This research investigated two factors: levels of Cd concentration (0, 15, and 30 mg kg−1) and type of biochar [control: without any biochar application, biochar (CB) and Fe-MCB]. Results indicated that the use of CB and Fe-MCB increased soil pH, the cation exchange capacity (CEC) of soil and soil organic matter content (SOM). The application of CB and Fe-MCB significantly decreased Cd bioavailability by 26.52 and 37.75%, respectively. The results of sequential extraction showed that the exchangeable and carbonate fractions of Cd decreased while organically bounded Cd and Fe/Mn-oxide-bounded Cd increased after Fe-MCB was applied. The application of Fe-MCB increased the IR values, but decreased MF values, demonstrating a decrease in the mobility of Cd in the soil. Overall, this study showed that Fe-MCB application especially at high concentrations of Cd can effectively immobilise Cd, thus decreasing its mobility in contaminated soils and improving the quality of soil environment and reducing remediation costs.

An electrogenerated chemiluminescence aptasensor for lysozyme based on the interaction between Ru(bpy)3 2+ and cucurbit[8]uril.

Strong anodic Ru(bpy)3 2+ electrogenerated chemiluminescence (ECL) was obtained at a cucurbil[8]uril (CB[8]) modified electrode in neutral conditions without the need of an additional coreactant. An ECL aptasensor was fabricated based on the strong ECL emission as well as the host-guest interaction between DNA and CB[8]. Firstly, amino group-terminated complementary DNA (DNA-NH2 ) was firmly immobilized on CB[8]/glass carbon electrode, which could further increase ECL intensity. Then, a ferrocene group-terminated lysozyme aptamer (Fc-DNA) was hybridized with complementary DNA. The inhibiting effect of ferrocene on Ru(bpy)3 2+ ECL resulted in the apparent decrease in ECL signal. When the modified electrode was incubated in lysozyme, specific binding between lysozyme and its aptamer could release the ferrocene group from the electrode surface, and the ECL emission was recovered. As a result, an 'on-off-on' mode ECL aptasensor for lysozyme was fabricated. In the range 0.14-140 pg ml-1 , the increased ECL intensities exhibited excellent linearity with the logarithm of lysozyme concentrations, and the detection limit was calculated as 0.093 pg ml-1 (3σ). The proposed ECL aptasensor exhibited satisfactory analytical performance, revealing the potential application of CB[n]s in an ECL sensing field.

Phytostabilization of Cd and Pb in Highly Polluted Farmland Soils Using Ramie and Amendments.

In-situ remediation of heavy-metal-contaminated soil in farmland using phytostabilization combined with soil amendments is a low-cost and effective technology for soil pollution remediation. In this study, coconut shell biochar (CB, 0.1% and 0.5%), organic fertilizer (OF, 3.0%), and Fe-Si-Ca material (IS, 3.0%) were used to enhance the phytostabilization effect of ramie (Boehmeria nivea L.) on Cd and Pb in highly polluted soils collected at Dabaoshan (DB) and Yangshuo (YS) mine sites. Results showed that simultaneous application of CB, OF, and IS amendments (0.1% CB + 3.0% OF + 3.0% IS and 0.5% CB + 3.0% OF + 3.0% IS, DB-T5 and DB-T6) could significantly increase soil pH, reduce the concentrations of CaCl2-extractable Cd and Pb, and increase the contents of Ca, P, S, and Si in DB soil. Under these two treatments, the growth of ramie was significantly improved, its photosynthesis was enhanced, and its levels of Cd and Pb were reduced, in comparison with the control (DB-CK). After applying DB-T5 and DB-T6, the concentrations of Cd and Pb in roots were decreased by 97.7–100% and 64.6–77.9%, while in shoots they were decreased by up to 100% and 92.9–100%, respectively. In YS-T4 (0.5% CB + 3.0% OF), the concentrations of Cd and Pb in roots were decreased by 39.5% and 46.0%, and in shoots they were decreased by 44.7% and 88.3%. We posit that phytostabilization using ramie and amendments could reduce the Cd and Pb bioavailability in the soil mainly through rhizosphere immobilization and plant absorption. In summary, this study suggests that the use of tolerant plant ramie and simultaneous application of coconut shell biochar, organic fertilizer, and Fe-Si-Ca materials is an effective stabilization strategy that can reduce Cd and Pb availabilities in soil. Ultimately, this strategy may reduce the exposure risk of crops to heavy metal pollution in farmland.

Evaluation of cell and hemocompatibility of Cucurbiturils

BackgroundCucurbiturils (CB) are pumpkin-shaped macrocyclic molecules consisting of methylen-bridged glycoluril units. Because of their complexing characteristics, they can be used as drug containers for medical purposes. For future biomedical and dermal application of CB, the investigation of cell compatibility is essential. Little is known about the influence of CB on eukaryotic cells, especially on dermal keratinocytes. The structurally related cyclodextrins are known to induce cell death by apoptosis in HaCaT keratinocytes as well as hemolysis in erythrocytes. ObjectiveTo examine cytotoxic effects of different CB. MethodsDifferent cytotoxicity tests were performed on HaCaT keratinocytes and erythrocytes incubated with CB[5], CB[6], and CB[7]. ResultsCB[5] and CB[6] did not lead to cytotoxic reactions at high concentrations up to 30 mg/mL whereas incubation with CB[7] triggered apoptosis at a concentration of 3.75 mg/mL. None of the investigated CB caused hemolytic effects on erythrocytes. ConclusionThese results confirm the high potential of CB as host-complexes for biomedical and dermal applications.

Cyanobacteria and Glutathione Applications Improve Productivity, Nutrient Contents, and Antioxidant Systems of Salt-Stressed Soybean Plant

Salt stress restricts plant performance by disrupting various physio-biochemical processes like photosynthesis. Plants growing in saline substrates show deficiencies in absorption of some essential elements due to the presence of excessive sodium (Na+) in the rhizosphere, which antagonizes beneficial cations and causing toxicity in metabolism. Cyanobacteria (CB; a natural biofertilizer) play a fundamental role in building-up soil fertility, thus increasing plant performance. Glutathione (GSH) is a well-known antioxidant, which contributes to increase salt tolerance in the plant. This work was conducted as a pot experiment (sand culture) in 2017 to study the combined effect of CB, applied as seed inoculation, and GSH, applied as foliar spray, on growth, pods and seed yields, the contents of antioxidants, osmoprotectants, and nutrients, and the antioxidative enzymes activities of soybean (Glycine max L., cv. Giza 111) plants grown under saline conditions. At fourth leaf stage (21 days after sowing; DAS), CB-pretreated seedlings were supplemented with NaCl (150 mM) along with Hoagland′s nutrient solution, and at the same time seedlings were sprayed with 1 mM GSH. Samples were taken at 60 DAS to assess morphological, physio-biochemical and antioxidant defense systems attributes. Results showed that the integrative application of CB and GSH under saline conditions was effective in improving significantly the growth characteristics, yield components, photosynthetic efficiency (pigments contents and chlorophyll fluorescence), membrane stability index, relative water content, contents of soluble sugars, free proline, ascorbic acid, glutathione, α-tocopherol, and protein, and activities of superoxide dismutase, catalase, and guaiacol peroxidase. The contents of macronutrients (N, P, K+, and Ca2+) were also increased significantly in Glycine max plants compared to the stressed control. In contrast, Na+ content and electrolyte leakage were significantly reduced. Our results recommend using the combined CB (as seed inoculation) and GSH (as foliar spray) application for soybean plantss to grow well under saline conditions.

Cyanobacteria and Glutathione Applications Improve Productivity, Nutrient Contents, and Antioxidant Systems of Salt-Stressed Soybean Plant

Salt stress restricts plant performance by disrupting various physio-biochemical processes like photosynthesis. Plants growing in saline substrates show deficiencies in absorption of some essential elements due to the presence of excessive sodium (Na+) in the rhizosphere, which antagonizes beneficial cations and causing toxicity in metabolism. Cyanobacteria (CB; a natural biofertilizer) play a fundamental role in building-up soil fertility, thus increasing plant performance. Glutathione (GSH) is a well-known antioxidant, which contributes to increase salt tolerance in the plant. This work was conducted as a pot experiment (sand culture) in 2017 to study the combined effect of CB, applied as seed inoculation, and GSH, applied as foliar spray, on growth, pods and seed yields, the contents of antioxidants, osmoprotectants, and nutrients, and the antioxidative enzymes activities of soybean (Glycine max L., cv. Giza 111) plants grown under saline conditions. At fourth leaf stage (21 days after sowing; DAS), CB-pretreated seedlings were supplemented with NaCl (150 mM) along with Hoagland′s nutrient solution, and at the same time seedlings were sprayed with 1 mM GSH. Samples were taken at 60 DAS to assess morphological, physio-biochemical and antioxidant defense systems attributes. Results showed that the integrative application of CB and GSH under saline conditions was effective in improving significantly the growth characteristics, yield components, photosynthetic efficiency (pigments contents and chlorophyll fluorescence), membrane stability index, relative water content, contents of soluble sugars, free proline, ascorbic acid, glutathione, α-tocopherol, and protein, and activities of superoxide dismutase, catalase, and guaiacol peroxidase. The contents of macronutrients (N, P, K+, and Ca2+) were also increased significantly in Glycine max plants compared to the stressed control. In contrast, Na+ content and electrolyte leakage were significantly reduced. Our results recommend using the combined CB (as seed inoculation) and GSH (as foliar spray) application for soybean plantss to grow well under saline conditions.

A new experimental model of intrinsic denervation in ileum from wistar rats through intramural microinjections of benzalkonium chloride

ABSTRACT: Extensive literature is available about the intrinsic denervation of segments of the digestive tube through the application of CB in the serosa of the viscera. However, this technique has some disadvantages like causing peritonitis, flanges and high mortality, limiting its use in humans. The aim of the present study was to evaluate the feasibility of benzalkonium chloride (CB) to induce intrinsic chemical denervation, through applications of CB in the intramural ileum of wistar rats, as well as deepen the knowledge about the evolution of neuronal injury caused in the process. We used 40 rats, divided into two groups (control-GC and benzalkonium-GB) of 20 animals each, divided into four sub-groups according to the time of postoperative assessment of 24, 48 hours, 30 and 90 days. The animals were submitted to intramural microinjections of sterile saline solution 0.9% (GC) or benzalkonium chloride (GB) in ileal portion, and subsequent histopathological analysis and immunohistochemistry for evaluation of neuronal injury. A significant decrease (p<0.05) was found of the neuronal myenteric count over time in groups, GB3, GB4 and GB2. The specific positive immunolabeling for H2AX and Caspase-3 confirmed the results obtained in the histopathological evaluation, denoting the ignition of irreversible cell injury in 24 hours, evolving into neuronal apoptosis in 48 hours after application of the CB 0.3%. Under the conditions in which this work was conducted, it can be concluded that the application of CB 0.3% by means of microinjections intramural in the ileal wall is able to induce intrinsic chemical denervation of the diverticulum of wistar rats and that the main mechanism of neuronal death is induction of apoptosis.

Releasing Metal-Coordination Capacity of Cucurbit[6]uril Macrocycle in Pseudorotaxane Ligands for the Construction of Interwoven Uranyl-Rotaxane Coordination Polymers.

As an emerging type of actinide hybrid material, uranyl-rotaxane coordination polymers (URCPs) with new coordination patterns and topological structures are still desired. In this work, we propose a new strategy to construct URCPs by promoting the simultaneous coordination of both the wheel and axle moieties in pseudorotaxane linkers with metal nodes. Starting from a series of cucurbit[6]uril (CB[6])-based pseudorotaxane ligands, C nBPCA@CB[6] [C nBPCA = 1,1-(α,ω-diyl)bis[4-(ethoxycarbonyl)pyridin-1-ium] bromides, where n = 5-8] with slightly deformed CB[6], four new URCPs (URCP1, URCP3, URCP4, and URCP5) with interwoven network structures, as well as another noninterwoven polymer(URCP2), have been successfully prepared. According to single-crystal structure analysis, we attribute the interwoven structures of the URCPs to the distortion of CB[6] in pseudorotaxane ligands with shorter or longer spacers (C5, C7, and C8). This indicates that the deformation could effectively diminish the steric hindrance around the portals, thus endowing the "inert" CB[6] host with coordination ability like the string molecule. Besides, the participation of water molecules and sulfate anions in the uranyl coordination sphere is also found to have a great influence on the final structures of the obtained URCPs. The successful preparation of interwoven URCPs in this work gives some new insights into the metal coordination of supramolecular entities and could facilitate other new applications of CB[6]-based pseudorotaxane ligands. Most importantly, the strategy proposed in this work provides some hints in the controllable design of metal-organic rotaxane frameworks with unique topologies.

A systematic evaluation of the biocompatibility of cucurbit[7]uril in mice

As one of the most water-soluble members in the macrocyclic cucurbit[n]uril (CB[n]) family, CB[7] has attracted increasing attention in pharmaceutical and biomedical fields. Despite extensive studies regarding the potential use of CB[7] for biomedical applications, its full safety and toxicity profile in a clinically relevant model is still lacking. Herein we report the full biocompatibility profile of CB[7], administered orally, peritoneally or intravenously in mice, respectively. Body-weight changes showed no significant differences among various groups of mice after they were administered with CB[7] at a single dose of 5 g/kg orally, 500 mg/kg peritoneally and 150 mg/kg intravenously, respectively. Hematology tests, as well as hepatic and renal function biochemical markers tests, of the blood collected from these mice sacrificed 21 days after CB[7] administration all exhibited normal ranges of values that were comparable with those of the control group. Moreover, histopathological analysis on the sections of major organs (including the heart, liver, spleen, lungs and kidneys) and gastrointestinal tissues revealed no detectable injuries and inflammatory cells infiltration. Taken together, these results suggest an excellent biocompatibility profile of CB[7] in mice, which provide important foundations for further investigations and even clinical applications of CB[7] in biomedical areas.

Applications of Cucurbit[n]urils (n=7 or 8) in Pharmaceutical Sciences and Complexation of Biomolecules

AbstractCucurbit[7]uril (CB[7]) and cucurbit[8]uril (CB[8]) are two key members of the cucurbit[n]uril (CB[n]) family of macrocycles. Because of the good water solubility of CB[7] and the unique ternary binding properties of CB[8], these two macrocycles have attracted increasing attentions in recent years. In particular, many promising reports of exciting applications regarding CB[7] and CB[8] have emerged in the pharmaceutical sciences and complexations of biomolecules, which has become one of the most important areas of potential applications of CB[n]s. This review summarizes the applications of macrocyclic CB[7], CB[8] and their derivatives as supramolecular platforms that have been developed in recent years within the field of pharmaceutical sciences and biomolecular sciences, and discusses the current challenges and future prospects of this area.

Cucurbit[7]uril: an emerging candidate for pharmaceutical excipients

Cucurbit[7]uril (CB[7]), belonging to the cucurbit[n]uril family (CB[n], n = 5-8, 10, or 13-15), may form host-guest complexes with a variety of small molecules of biomedical interest. The physical and chemical properties of the complexed drugs are often improved as a result of this complexation, suggesting the potential application of CB[7] as a pharmaceutical excipient. This review has summarized the most recent research progress reported between 2011 and early 2017 regarding the biocompatibility of CB[7] and the influence of CB[7] on the stability, solubility, biouptake, and biological activities (including therapeutic efficacies and toxicities) of guest drug molecules. Through this systemic summary and analysis, we intend to stimulate further research efforts in this area and promote the use of CB[7] as an emerging pharmaceutical excipient to improve various properties of drug molecules (or active pharmaceutical ingredients).

Pulse Duration as Well as Current Direction Determines the Specificity of Transcranial Magnetic Stimulation of Motor Cortex during Contraction.

Previous research suggested that anterior-posterior (AP) directed currents induced by TMS in motor cortex (M1) activate interneuron circuits different from those activated by posterior-anterior currents (PA). The present experiments provide evidence that pulse duration also determines the activation of specific interneuron circuits. To use single motor unit (SMU) recordings to confirm the difference in onset latencies of motor-evoked potentials (MEPs) evoked by different current directions and pulse durations: AP30, AP120, PA30 and PA120. To test whether the amplitude of the MEPs is differentially influenced by somatosensory inputs from the hand (short-latency afferent inhibition, SAI), and examine the sensitivity of SAI to changes in cerebellar excitability produced by direct current stimulation (tDCSCb). Surface electromyograms and SMUs were recorded from the first dorsal interosseous muscle. SAI was tested with an electrical stimulus to median or digital nerves ~20-25 ms prior to TMS delivered over the M1 hand area via a controllable pulse parameter TMS (cTMS) device. SAI was also tested during the application of anodal or sham tDCSCb. Because TMS pulse specificity is greatest at low stimulus intensities, most experiments were conducted with weak voluntary contraction to reduce stimulus threshold. AP30 currents recruited the longest latency SMU and surface MEP responses. During contraction SAI was greater for AP30 responses versus all other pulses. Online anodal tDCSCb reduced SAI for the AP30 currents only. AP30 currents activate an interneuron circuit with functional properties different from those activated by other pulse types. Pulse duration and current direction determine what is activated in M1 with TMS.

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion

Topical combination of a vitamin D3 analogue and corticosteroid is widely used for the treatment of psoriasis, a TH17-mediated disorder, but the underlying mechanism remains unclear. We investigated the effect of this topical applicant, focusing on skin-infiltrating TH17cells. In 10 patients with plaque psoriasis, calcipotriol (Cal), betamethasone dipropionate (Bet), or the calcipotriol and betamethasone dipropionate 2-compound formulation (CB) was applied to 3 different psoriatic plaques with similar severity once a day for 14days. One nonapplied lesion was used as a control. Four-millimeter biopsy specimens were taken from each site, cut into 2 pieces, and subjected to histologic examination and exvivo expansion of skin-infiltrating Tcells with anti-CD3/CD28 antibodies and IL-2. Clinical, histologic, and IL-17A(+) cell-infiltrate improvement was found in the following order: CB>Cal>Bet>control or CB>Bet>Cal>control. Numbers of exvivo expanded Tcells were decreased by topical application of Bet and CB, and CB exhibited the most suppressive result. Numbers and frequencies of TH17cells were significantly reduced by CB and Cal, suggesting that Cal has a capacity to preferentially suppress TH17cells. When the stocked Tcells from control samples were stimulated with anti-CD3 antibodies in the presence of Bet, Cal, or both, Cal downmodulated IL-17 and IFN-γ production and tended to upregulate IL-4 and IL-6 without apoptosis, but Bet inhibited production of these cytokines with apoptosis. These findings suggest that Cal and Bet have different effects on Tcells to normalize psoriatic changes, with decreased TH17cell expansion in the skin lesions.

Interactions of cucurbit[6,7]urils with human serum albumin and their effects on zaltoprofen transportation

Interference of CB[6,7]s on a HSA–zaltoprofen binding system was studied for conducting the applications of CB[6,7]s.

Short-Term Effects of Different Organic Amendments on Soil Properties and Organic Broccoli Growth and Yield

ABSTRACTThe aim of this study was to evaluate the effects of different organic fertility sources on organic broccoli (Brassica oleracea var. italica) growth and yield, and on soil properties. The fertilization was designed to supply the crop with about 100 kg ha−1 of N using two amendments: a municipal solid wastes-based compost (CC), and a manure based compost (CB). The former was used alone (T1), in combination with its own water extract (CT) in fertigation (T2), and in combination to CT applied in fertigation and as foliar spray (T3). Other treatments resulted from the application of (i) CC and CB (T4); (ii) CB alone (T5); and (iii) the exhausted compost (EX) resulting from the extraction of CT (T6). Unamended plots were used as control (T7). Selected soil properties were determined at the beginning and at the end of the trial in order to estimate the effects of the various treatments on these parameters. The best yields were obtained with T4 and T5 (16.95 and 18.46 t ha−1, respectively). The application of CC alone (T1) showed slightly better growing parameters in comparison to its application in combination to CT (T2 and T3), even though the yields were not significantly different from each other. T6 showed the lowest yield of broccoli (7.03 t ha−1) among the treatments, superior only to the unfertilized control (5.38 t ha−1, T7). The different fertilizations showed a general soil enrichment of available phosphorous and organic matter, even if the latter could be only transitory. Finally, the high production costs and the low yields obtained apparently dissuade the use of compost tea and exhausted compost for the fertilization of broccoli crop.