Application Of Biosensor For Detection Research Articles

A systematic review on recent trends and perspectives of biosensors in food industries

AbstractThe food industry has inappropriate techniques for process and quality control and requires techniques that can provide information on the physical and chemical properties of food quickly and affordably. Food applications mostly focus on the identification of contaminants, with a few significant analytes, such as sugars, alcohols, amino acids, flavors, and sweeteners, as exceptions. Biosensors can bring about an analytical revolution to address the problems facing the food and agriculture industries. Potential applications for biosensors include contaminant detection, product freshness monitoring, content verification, and raw material conversion monitoring. These studies are costly and time‐consuming due to the extraction or pre‐treatment of samples. This review gives an overview of biosensors with their classification and application in different food industries such as the fruits and vegetable industry, dairy, and meat industries. The application of biosensors in detection, quality assurance, and food safety is discussed in detail with their potential application.

Application of biosensors for detection of meat species: A short review

Biosensors are easy-to-use tools that have made many advances in recent years. In the meat industry, most of these devices are applied in pathogen detection or freshness analysis. However, they are also applicable in the investigation of adulteration in meat by mixtures of different animal species, since the authenticity of food components is a serious problem involving health, economic, legal and religious concerns. This review aimed to summarize the biosensors developed for this purpose and how they manage to detect different meat species in complex mixtures. Only 41 studies were found, the majority involving optical biosensors, from which 63% aimed the detection of pork in meat samples. However, for an appropriate sensitivity, 81% of the proposed devices required extensive purification and/or amplification steps of the samples, unsuitable to point-of-need use. In view of this and the absence of studies on the stability of the platforms over time, it is necessary to develop more robust devices that can be used at any stage of the meat industrial chain and even by the final consumer.

Protein based biomarkers for non-invasive Covid-19 detection

COVID-19 has become a substantial lethal disease worldwide, and early diagnosis is a significant concern for this virus. Currently, RT-PCR is being used worldwide for the detection of this virus with human to human transmission. Furthermore, the recent develop biosensor leading to others diagnosis approach but being invasive are painful and time taking. Another possibility can be protein-based biomarkers as an application of biosensors for detection and early diagnostics. Considering the other approach, that is, microfluidics-based biosensor, though being a non-invasive method, will be restricting virus transmission. This review commences with the recent develop biosensor for Covid-19 detection and listing down the available biomarkers with their secretion range comparison of normal to COVID-19 patients through clinical analysis in china and concludes with the future approach for the diagnosis.

Application of Biosensors for Detection of Pathogenic Food Bacteria: A Review.

The use of biosensors is considered a novel approach for the rapid detection of foodborne pathogens in food products. Biosensors, which can convert biological, chemical, or biochemical signals into measurable electrical signals, are systems containing a biological detection material combined with a chemical or physical transducer. The objective of this review was to present the effectiveness of various forms of sensing technologies for the detection of foodborne pathogens in food products, as well as the criteria for industrial use of this technology. In this article, the principle components and requirements for an ideal biosensor, types, and their applications in the food industry are summarized. This review also focuses in detail on the application of the most widely used biosensor types in food safety.

Application of a Plasmonic Biosensor for Detection of Human-Liver Tissues

A recently published plasmonic biosensor based on birefringent solid-core microstructured optical fiber is applied for detection of five types of human-liver tissues (normal N, metastatic MET, non-cancerous metastatic (NMET), hepatocellular carcinoma (HCC), and non-cancerous hepatocellular carcinoma (NHCC)). The birefringent behavior is obtained by removing five central air holes of a two-ring hexagonal lattice of holes in a gold covered silica fiber with the liver layer surrounding the fiber. The loss spectra show two resonant peaks corresponding to the phase matching points. To distinguish between normal and malignant liver tissues, we compare the relevant parameter for the type I and II core modes. Thus, for a decrease of the real part of the refractive index from 1.373431 (human-liver N) to 1.354602 (human-liver MET), the resonance spectral width δλ 0.5 is increased from 44.3 to 54.2 nm for the core mode II. In addition, the amplitude sensitivity S A is decreased from 158.6 to 88.4 RIU−1 for the same core mode. The advantages of another recently proposed plasmonic sensor based on a Bragg fiber are the larger values of the transmission loss, maximum value of the amplitude sensitivity, figure of merit, power fraction in a normal liver layer, and power fraction in the gold layer as compared with that for a microstructured fiber when applied for detection of a normal liver tissue. Another advantage of the Bragg fiber is related to the smaller value of the difference between maximal amplitude sensitivity and resonant wavelengths.

Label Free Inhibitor Screening of Hepatitis C Virus (HCV) NS5B Viral Protein Using RNA Oligonucleotide

Globally, over 170 million people (ca. 3% of the World’s population) are infected with the hepatitis C virus (HCV), which can cause serious liver diseases such as chronic hepatitis, evolving into subsequent health problems. Driven by the need to detect the presence of HCV, as an essential factor in diagnostic medicine, the monitoring of viral protein has been of great interest in developing simple and reliable HCV detection methods. Despite considerable advances in viral protein detection as an HCV disease marker, the current enzyme linked immunosorbent assay (ELISA) based detection methods using antibody treatment have several drawbacks. To overcome this bottleneck, an RNA aptamer become to be emerged as an antibody substitute in the application of biosensor for detection of viral protein. In this study, we demonstrated a streptavidin-biotin conjugation method, namely, the RNA aptamer sensor system that can quantify viral protein with detection level of 700 pg mL−1 using a biotinylated RNA oligonucleotide on an Octet optical biosensor. Also, we showed this method can be used to screen inhibitors of viral protein rapidly and simply on a biotinylated RNA oligonucleotide biosensor. Among the inhibitors screened, (−)-Epigallocatechin gallate showed high binding inhibition effect on HCV NS5B viral protein. The proposed method can be considered a real-time monitoring method for inhibitor screening of HCV viral protein and is expected to be applicable to other types of diseases.