Application Of 6-benzylaminopurine Research Articles

Growth and biochemical responses of red chili (Capsicum annuum L) under drought conditions with 6-Benzylaminopurine application

The purpose of this research is to assess how red chili plants (Capsicum annuum L.) develop and react biochemically to drought stress, as well as how cytokinin treatment affects these plants. The study employed a factorial Randomized Block Design (RAK) with three replications and two components, namely the degree of drought with three stages, comprising: K1 has an 80% soil water content, K2 has a 60% soil water content, K3 has a 40% soil water content, and S0, S1, S2, and S3 have 0 ppm, 10 ppm, 20 ppm, and 30 ppm of 6-benzylaminopurine concentration, respectively. Plant height, leaf count, root length, flowering age, total and aqueous chlorophyll content, activity of antioxidant enzymes (e.g., superoxide dismutase and peroxide dismutase), and hydrogen peroxide as a signal for plant molecules against dehydration stress are among the parameters assessed. The findings demonstrated that red chili plants under drought stress experienced slower growth, as seen by a reduction in height and leaf count as well as earlier flowering. However, by raising plant height, leaf count, and chlorophyll levels (a, b, and total), cytokinin treatment was able to lessen the deleterious impacts of drought. When treated with 10 ppm 6-Benzylaminopurine, the enzyme activity of superoxide dismutase, peroxide dismutase, and hydrogen peroxide increased, but at other dosages, it tended to decrease, suggesting a slight but noticeable increase in plant defense mechanisms against oxidative stress. Therefore, giving red chili plants 10 parts per million of cytokinin may be a useful tactic for enhancing their resistance to drought stress.

Application of Benzylaminopurine with Methyl Jasmonate and Epibrassinolide Improved Growth and Physio-biochemical Attributes of Strawberry (Fragaria × ananassa cv. ‘Albion’)

Application of Benzylaminopurine with Methyl Jasmonate and Epibrassinolide Improved Growth and Physio-biochemical Attributes of Strawberry (Fragaria × ananassa cv. ‘Albion’)

Postharvest starch and sugars adjustment in potato tubers of wide-ranging dormancy genotypes subjected to various sprout forcing techniques

The development of an efficient, safe, and environment-friendly technique to terminate tuber dormancy in potatoes (Solanum tuberosum L.) is of great concern due to the immense scope of multiple cropping all over the globe. The breakage of tuber dormancy has been associated with numerous physiological changes, including a decline in the level of starch and an increase in the levels of sugars during storage of freshly harvested seed potatoes, although their consistency across genotypes and various dormancy-breaking techniques have not yet been fully elucidated. The purpose of the present research is to assess the efficacy of four different dormancy-breaking techniques, such as soaking in 90, 60, or 30 mg L−1 solutions of benzyl amino purine (BAP) and 30, 20, or 10 mg L−1 gibberellic acid (GA3) alone and in the combination of optimized concentrations; cold pre-treatment at 6, 4, or 2 °C; electric shock at 80, 60, 40, or 20 Vs; and irradiation at 3.5, 3, 2.5, 2, 1.5, or 1 kGy on the tuber dormancy period and sprout length of six genotypes. Furthermore, the changes that occurred in tuber weight and endogenous starch, sucrose, fructose, and glucose contents in experimental genotypes following the application of these techniques were also examined. Overall, the most effective technique to terminate tuber dormancy and hasten spout growth was the combined application of BAP and GA3, which reduced the length of dormancy by 9.6 days compared to the untreated control, following 6.7 days of electric current, 4.4 days of cold pre-treatment, and finally irradiation (3.3 days). The 60 mg L−1 solution of BAP greatly reduced the dormancy period in all genotypes but did not affect the sprout length at all. The genotypes showed a weak negative correlation (r = − 0.4) (P < 0.05) of endogenous starch contents with dormancy breakage and weight loss or a moderate (r = − 0.5) correlation with sprout length, but a strong positive correlation (r = 0.8) of tuber glucose, fructose, and sucrose contents with dormancy breakage and weight loss. During 3 weeks of storage, sprouting commencement and significant weight loss occurred as tuber dormancy advanced towards breakage due to a reduction in starch and an increase in the sucrose, fructose, and glucose contents of the tubers. These findings could be advantageous for postponing or accelerating seed potato storage as well as investigating related physiological research in the future.

Lateral Shoot Induction with Exogenous 6-benzylaminopurine Application at the Vegetative Growth Stage in Phalaenopsis

Lateral Shoot Induction with Exogenous 6-benzylaminopurine Application at the Vegetative Growth Stage in Phalaenopsis

The Changes in Various Physio-Biochemical Parameters and Yield Traits of Faba Bean Due to Humic Acid Plus 6-Benzylaminopurine Application under Deficit Irrigation

Implementing the deficit irrigation pattern has become a major strategy in crop production systems. However, using less water than is required to irrigate crops is associated with changes in plant physiology and lower productivity. Therefore, the current research aimed to assess the integrated effect of humic acid and cytokinin on faba bean under water deficit. Under two irrigation levels (full irrigation, FI and deficit irrigation, DI), two humic acid treatments (without addition, H0 and with addition of 10 kg ha−1, H10) and two cytokinin concentrations (without spray, C0 and spraying with 25 mg L−1, C25), faba bean growth, physiology, and productivity were evaluated. The experiment was implemented for two winter seasons of 2019/20 and 2020/21 and performed in a split–split plots design with three replicates. The findings revealed that under low water supply (DI), H10 plus C25 was the most efficient treatment for enhancing faba bean growth. All physiological faba bean traits estimated under DI showed remarkable increases with the application of H10 plus C25 in both seasons. The increases in proline, catalase, and total soluble sugars under DI due to H10 plus C25 were 31.4 and 31.8%, 51.9 and 55.1% as well as 43.8 and 46.6%, in the first and second seasons, respectively. There was no significant difference between FI × H10 plus C25 and DI × H10 plus C25 in phosphorus content in both seasons. FI × H10 plus C25 and DI × H10 plus C25 in the second season produced a similar number of pods plant−1 and seed yield of faba bean. Conclusively, the combined application of humic plus cytokinin achieved physiological and nutrient homeostasis, adjusting the biochemical compounds in faba bean under water deficit.

Effects of 6-Benzylaminopurine Combined with Prohexadione-Ca on Yield and Quality of Chrysanthemum morifolium Ramat cv. Hangbaiju

Increasing shoot branch numbers of Chrysanthemum morifolium Ramat cv. Hangbaiju (Hangbaiju) is crucial for producing high flower yields. Pot experiments were designed to evaluate the effects of foliar application of 6-benzylaminopurine (6-BA) combined with prohexadione-Ca (Pro-Ca) on the yield and quality of Hangbaiju flowers. Foliar application of 6-BA combined with Pro-Ca typically increased leaf chlorophyll content and decreased leaf soluble sugar and soluble protein contents throughout the floral organ growth phase. At the bud formation phenophase stage (August), the contents of gibberellin (GA), indole-3-acetic acid (IAA), and zeatin (ZA) decreased (except for GA content in the 10 mg L−1 6-BA combined with 100 mg L−1 Pro-Ca treatment), but abscisic acid (ABA) content increased. The yield of Hangbaiju flowers was found higher in plants treated with foliar application of 6-BA combined with Pro-Ca. Higher yields were found in the 6-BA5 + Ca100 and 6-BA10 + Ca100 treatments than in the 6-BA5 + Ca50 and 6-BA10 + Ca50 treatments, and the highest yield was observed in the 6-Ba5 + Ca100 treatment both in 2019 and 2020. Nutritional indices such as soluble sugar, soluble protein, total amino acid, and water extract increased, and medicinal indices such as flavonoid, total phenolics, chlorogenic acid, cynaroside, and 3,5-dicaffeoyl quinic acid slightly decreased after foliar application of 6-BA combined with Pro-Ca; however, they were all higher than the standards recorded in the Chinese Pharmacopeia. Overall, foliar application of 6-BA combined with Pro-Ca could increase the yield and nutritional quality of Hangbaiju flowers, enhancing its tea quality.

Paclobutrazol and benzylaminopurine improve potato yield grown under high temperatures in lowland and medium land

Abstract Potato is one of the most important vegetable crops in the world. In a tropical country such as Indonesia, potato is cultivated in highland that has low temperature. However, the extensification and intensification of potato cultivation cause environmental problems in the highland. Soil erosion is one of the common problems resulting from potato cultivation that applies heavy tillage. To prevent environmental problems, the land expansion for potato cultivation is carried out in both the medium and lowland. High temperature in both medium and lowland results in the limitation of plant growth and yield. Therefore, the present study aimed to evaluate the effect of plant growth regulators (PGRs), namely paclobutrazol and benzylaminopurine (BAP) application on the growth and yield of potatoes grown under high temperature in both lowland and medium land. A split-plot design was used in this experiment with the main plot as growing altitude, i.e., low and medium land, and the PGR treatments as the subplot, i.e., control, paclobutrazol at 100 mg L−1, BAP at 50 mg L−1, and a combination of paclobutrazol at 100 mg L−1 and BAP at 100 mg L−1. The result showed a reduction in plant growth and yield in potatoes grown in lowland compared to those in medium land. The application of paclobutrazol and BAP improved the number, the weight of tuber, and its starch content. The result indicated that the application of paclobutrazol and BAP could be used to solve an environmental limitation for potato cultivation in both lowland and medium land.

Cyclanilide Induces Lateral Bud Outgrowth by Modulating Cytokinin Biosynthesis and Signalling Pathways in Apple Identified via Transcriptome Analysis.

Cyclanilide (CYC), a plant growth regulator, is a potent shoot branching agent in apple. However, its mechanism remains unclear. The current study revealed that CYC treatment resulted in massive reprogramming of the axillary bud transcriptome, implicating several hormones in the response. We observed a marked increase (approximately 2-fold) in the level of zeatin riboside and a significant decrease (approximately 2-fold) in the level of abscisic acid (ABA). Zeatin metabolism gene cytokinin (CTK) oxidase 1 (CKX 1) was down-regulated at 168 h after CYC treatment compared with the control. Weighted gene co-expression network analysis of differentially expressed genes demonstrated the turquoise module clusters exhibited the highest positive correlation with zeatin riboside (r = 0.92) and the highest negative correlation with ABA (r = −0.8). A total of 37 genes were significantly enriched in the plant hormone signal transduction pathway in the turquoise module. Among them, the expressions of CTK receptor genes WOODEN LEG and the CTK type-A response regulators genes ARR3 and ARR9 were up-regulated. ABA signal response genes protein phosphatase 2C genes ABI2 and ABI5 were down-regulated in lateral buds after CYC treatment at 168 h. In addition, exogenous application of 6-benzylaminopurine (6-BA, a synthetic type of CTK) and CYC enhanced the inducing effect of CYC, whereas exogenous application of lovastatin (a synthetic type of inhibitor of CTK biosynthesis) or ABA and CYC weakened the promoting effect of CYC. These results collectively revealed that the stimulation of bud growth by CYC might involve CTK biosynthesis and signalling, including genes CKX1 and ARR3/9, which provided a direction for further study of the branching promoting mechanism of CYC.

Hormonal endogenous changes in response to the exogenous 6-benzylaminopurine application in pre- and post-harvesting lilium flower stalks

Abstract Phyto-hormones play a key role in regulating plant responses to stress. Cytokines are a type of phyto-hormones involved in the regulation of many important biological processes related to growth, development, and response to environmental variables. The exogenous application of cytokines increases the possibility of delaying senescence; however, this is a physiological process, and, under certain conditions, degradation processes may be triggered. The effect of 6-bencilaminopurine application and the endogenous hormonal changes involved in lilies floral stalks after their cutting were studied. In order to improve vase life and quality of Lilium longiflorum ‘Brindisi’ flower stalks, they were sprayed with 6-BAP, at a concentration of 300 ppm at pre-harvest, post-harvest, and pre- and post-harvest stages. After that, they were compared to non-sprayed control stalks. The application of 6-BAP caused endogenous hormonal changes in abscisic acid and cytokinin levels, and the most effective treatment was pre-harvest spraying. This treatment proved to be an appropriate method to improve the stalk tolerance to post-harvest stress as it delayed the appearance of senescence symptoms and reduced the speed of chlorophyll degradation with differences of up to 10% with respect to untreated stalks. In addition, the opening of flowers was delayed by up to 2 days, although there were no significant differences in total vase life.

6-Benzylaminopurine application enhances petal coloration of pink flowered chrysanthemums

6-Benzylaminopurine application enhances petal coloration of pink flowered chrysanthemums

Efecto de la desinfección con hipoclorito de sodio y multiplicación con bencilaminopurina en Musa spp.

Dentro de la familia de las Musaceas, el género Musa ha adquirido importancia en la alimentacion a nivel mundial, ya sea por el consumo directo (fruta) o indirecto (previa cocción) como es en caso de Bolivia, sin embargo por cambio de actividades agroeconómicas este rubro esta siendo afectado, y tambien carecen de un mecanismo de regeneracion de los plantines libre de enfermedades. El presente estudio tuvo como objetivo estandarizar la desinfección de hijuelos de Gran enano (banano) y Harton (plátano) con diferentes concentraciones de hipoclorito de sodio (NaClO) e inducir la multiplicación con la aplicación de bencilaminopurina. Los hijuelos de ambas variedades fueron sometidas a dos tiempos de desinfección, el primero consistió en seleccionar los hijuelos de 10 a 12 cm de longitud y sumergirlas en hipocloriro de sodio al 8 % durante 12 min y posteriormente, se dividio en dos grupos para la segunda desinfeccion de 2 y 4 % con hipoclorito de sodio durante 7 min, luego fueron enjuagadas y sembradas en el medio Murashige y Skoog (MS) con 2 mg L-1 BAP (bencilaminopurina) + 1 mg L-1 ANA (ácido naftalenacético). En la fase de multiplicación los brotes se sembraron en medios de cultivo (MC1 = MS + 2.5 mg L-1 BAP + 1 mg L-1 ANA) y (MC2 = MS + 5 mg L-1 BAP + 1 mg L-1 ANA). Se determinó que la combinacion de una primera y segunda desinfección 8 y 4 % de NaClO desinfectó un 80 % en Gran enano y 40 % en Hartón respectivamente. En la fase de multiplicación se determinó que el medio de cultivo MS suplementado con 5 mg L-1 de BAP favoreció en la formación de 8 brotes en banano en ambas variedades.

COMPARISON OF VARIOUS CHEMICAL TREATMENTS TO BREAK DORMANCY OF GLADIOLUS CORMS

Gladiolus is commercially propagated through corms and occurrence of dormancy in freshly harvested corms creates hindrance in their cultivation. Present study was designed to compare the effect of different chemicals to alleviate the dormancy of corms in a short time. Gladiolus corms were collected from two growing sites and subjected to chemicals methanol, benzylaminopurine (BAP), gibberellic acid (GA3), indole acetic acid (IAA), salicylic acid, potassium nitrate and thiourea at different concentrations alone or in combinations. The treated corms were planted on sand and placed under dark conditions at 26°C. The treatment of 60% methanol in combination with 0.9 mM BAP showed maximum germination (72.71%). Descaled corms showed 85% germination compared to 60.42% scaled corms, while the corms collected from Faisalabad showed better germination 83.75% compared to corms from Rawalakot (61.67%). The application of 60% methanol also induced 29.65% and 42.43% deterioration in scaled and descaled corms, respectively. The treatments of 0.3 mM BAP in combination with 0.6 mM GA3 exhibited 88.33 % germination in corms collected from Faisalabad as compared to 55% in corms from Rawalakot. Overall, application of BAP and GA3 in combination was found effective in shortening the period of dormancy in both cultivars of gladiolus compared to other tested chemicals or growth regulators.

Influence of plant growth regulators on fatty acid composition of Simarouba glauca DC.

Simarouba glauca is a medicinally important oil yielding plant. It is a rainfed wasteland evergreen edible oil tree. Presowing soaked seeds of Simarouba glauca in various Plant Growth Regulators (PGRs) are analyzed to estimate their fatty acid composition. The fatty acids extraction was done using petroleum ether and fatty acid methyl esters (FAMEs) were analyzed by Gas Chromatography with Flame Ionization Detector (GC-FID). Due to the application of growth regulators stearic acid, lingoceric acid and linolenic acid enhances noticeably, while, total saturated fatty acids are augmented due to cysteine, Salicylic Acid (SA) and methionine treatments and monosaturated fatty acids elevated due to the application of 6-Benzylaminopurine (6-BA) whereas polyunsaturated fatty acids enhanced in response to Gibberellic Acid (GA) and Chlormequat chloride (CCC). The PGR induced changes in fatty acid composition predominantly in polyunsaturated fatty acids may certainly recover the oil quality of S. glauca seeds.

Changes of Growth and Inflorescence Initiation by Exogenous Gibberellic Acid3 and 6-Benzylaminopurine Application in Phalaenopsis Orchids

This study was conducted to observe the effects of exogenous gibberellic acid3 (GA3) and 6-benzylaminopurine (BAP) treatments on the growth and inflorescence initiation of Phalaenopsis and to determine whether hormonal applications can substitute for low-temperature exposure for floral transition. Phalaenopsis Queen Beer ‘Mantefon’ clones were treated with no hormones (control), GA3 100 mg/L, GA3 200 mg/L, BAP 100 mg/L, and GA3 100 mg/L + BAP 100 mg/L by foliar spray. The treatments were carried at 28 °C for vegetative growth and 20 °C for forcing, respectively. At 28 °C of vegetative temperature, all exogenous hormonal treatments did not induce inflorescence initiation, but lateral shoots were observed in BAP-treated plants even though this plant is a monopodial orchid. GA3 significantly increased leaf length and decreased leaf width, and consequently increased length:width (L:W) ratio compared with the control and BAP alone. The trend grew as GA3 concentration increased. Also, the GA3 increased stem length and decreased stem diameter. At 20 °C of forcing temperature, L:W ratio responded similarly to 28 °C in GA3 treatments, but leaf size was smaller than for the control or BAP alone. BAP accelerated inflorescence emergence and significantly increased inflorescence numbers, whereas GA3 and GA3 + BAP slightly delayed inflorescence emergence. GA3 significantly promoted new leaf development at 20 °C of forcing condition. These results indicated that cytokinin was associated with the break of axillary vegetative and inflorescence meristems and exogenous GA3 spray did not improve inflorescence initiation in Phalaenopsis. Although exogenous hormonal application did not substitute for low-temperature exposure, it showed a possibility in promoting the growth and inflorescence initiation.

Effect of benzyl amino purine and 1-methylcyclopropene in maintaining rooting quality of chrysanthemum (Chrysanthemum morifolium Ramat cv. ‘White Fiji’) cuttings

The quality of chrysanthemum cuttings is influenced by ethylene. During postharvest storage, ethylene accelerates the quality loss of cutting by enhancing chlorophyll degradation. Application of 1-Methylcyclopropene (1-MCP) or Benzyl Amino Purine (BAP) can be used as an approach to inhibit unfavourable ethylene effects. The objective of this study was to find out the effect of a combination of 1-MCP or BAP and storage period on the quality of chrysanthemum cuttings. The experiments were arranged using a completely randomized design consisting of nine treatments. The cuttings were treated with 1-MCP 700 nL/L or BAP 2.5 mg/l. After treatment, they were stored for 0, 3 and 7 days, then the cuttings were rooted in soilless culture for four weeks. The results showed that 1-MCP or BAP maintained leaf freshness and inhibited chlorophyll degradation on cuttings at 3 and 7 days of storage period. However, the application of 1-MCP slightly inhibited root growth compared to that of untreated cuttings. Interestingly, the application of BAP effectively reduced the cuttings damage for seven days and did not cause the inhibition of root growth (weight of fresh root, root number, root length and root volume) that was comparable to that of untreated cuttings. These results collectively showed that BAP was more effective than 1-MCP in maintaining rooting quality of chrysanthemum cuttings.

Application of Cytokinin and Mycorrhiza to Increase Production and Quality of Pineapple Seedlings from Crown Leaf Bud Cuttings

The pineapple vegetative propagation method using crown leaf bud cuttings has potential to produce seedlings of greater uniformity and in larger quantities than other methods. This study, aimed to explore the potential eff ects of concentration and frequency of Benzylaminopurine (BAP) application for increasing the production and quality of pineapple seedlings from crown leaf bud cutting propagation. The experiment used a randomized complete block design (RCBD) with two factors. The fi rst factor was the concentration of BAP (0, 200, 400, 600, 800 ppm) and the second factor was the frequency of BAP application (1, 2, 3 times). Furthermore, this study investigated the eff ects of applying mycorrhizae to accelerate seedling growth and improve seedling quality during production from crown leaf bud cutting propagation. The experiment used a RCBD with one factor (dose of mycorrhiza: 0, 50, 100, 150, 200 spores). The results indicated that the eff ects of concentration and frequency of BAP application and their interaction increased seed production as determined by sprout cuttings at 5 weeks after planting by applying 600 ppm 2 times. Generally, with increasing concentration and frequency of BAP application, leaf chlorophyll content was reduced. However, the application mycorrhiza positively infl uenced the production of seedlings from pineapple crown bud cuttings and improved seedling quality as measured by root dry weight, chlorophyll content, root infection, and phosphatase enzyme activity.

Response of some soybean (Glycine max L. Merrill) varieties on flooded conditon with application of Benzyl Amino Purine (BAP) and Salicylic Acid (SA) in the R3 phase

Soybean production (Glycine max (L.) Merril) in Indonesia has fluctuation every year. The area of wetlands in Indonesia is about 24.5 million ha, but only about 8.5 million ha of wetlands are used as rice fields, the remaining 16 million ha have not been utilized. The study aims to determine the response of some soybean varieties on the application of benzyl amino purine (BAP) and salicylic acid (SA) in flooded conditions. The research initiated in research land of the Faculty of Agriculture Universitas Sumatera Utara, using the Randomized Block Design (RBD) with 3 treatment factors namely varieties consisting of 3 varieties (Demas 1, Dering 1 and Burangrang), the concentration of plant growth regulator consisting of 3 levels (control, 50 ppm BAP + 100 ppm of SA and 100 ppm of BAP + 200 ppm of SA) and the treatment of flooding stress consisting of 2 treatments (control and flooded). The results showed that the varieties had critical effect on the parameters of number of pods per plant and the weight 100 seeds. Synthetic plant growth regulator concentration had no effect on all the observation parameters, but flooding stress had significant induce on the parameter of number of pods per plant.

Pengaruh Pemberian BAP dan NAA terhadap Pertumbuhan Krisan (Chrysanthemum morifolium, Ram.) dalam Kultur Jaringan

The objective of this study was to evaluate the effect of combination benzyl amino purine(BAP) and naphtalene acetid acid (NAA) on tissue culture of Chrysanthemum. Theexperiment was conducted at The Tissue Culture Laboratory, Agriculture Faculty,Stiper Agriculture Institute. The Completely Randomized Design was applied in thisexperiment, consisting of two factors; those were BAP and NAA application. Each factorconsisted of four treatments. Each combination of treatment was carried out with ninereplications. The conclusion from this study were: Application of higher BAP and NAAconcentration increased budding of explant, whereas application of higher NAA thanBAP concentration increased the growth of bud and leaf number, also increased rootingof explant.

Adaptability of Moringa oleifera Lam. under different water holding capacities

Growth performance and enzymatic-antioxidant activities of Moringa oleifera were investigated under simultaneous effect of water availability and plant growth regulators. Plant growth regulators i.e., cytokinin (50 mg L−1), thiourea (5 mM), benzyl amino purine (BAP @ 50 mg L−1), salicylic acid (50 mg L−1), hydrogen peroxide (120 μM) and ascorbic acid (50 mg L−1) were exogenously applied to moringa plants at three water holding capacity levels, 100, 70, and 40% in a completely randomized design with three replications. A decrease in shoot length, leaf score and number of branches was recorded at 100 and 40% water holding capacity as the plants respond well at 70% water holding capacity. BAP, cytokinin and salicylic acid were found as effective plant growth regulators in improving drought tolerance in moringa plants even at 40% water holding capacity. On the basis of these findings, moringa can be suggested to cultivate as a fodder crop under water deficit conditions and its tolerance can be further improved by foliar application of benzyl amino purine and ascorbic acid.

Pengaruh Pemangkasan dan Aplikasi Sitokinin terhadap Pertumbuhan dan Hasil Tanaman Teh (Camellia sinensis)

&lt;em&gt;Pruning on tea plants is an ecophysiological manipulation to initiate the growth of new shoots as the formation of pecco shoots. Cytokinin, one of which is benzyl amino purine (BAP), is a growth regulator that can be applied to stimulate shoot initiation after being pruned. The objective of this study was to determine the effect of pruning and cytokinin application on the growth and yield of tea. The study was conducted at the Experimental Station of the Tea and Quinine Research Center, Gambung at an altitude of 1250 m above sea level (asl), from June to August 2018. Plant material used in this study was a 7-year-old GMB 7 clone. The exerimental design used was a randomized block design (RBD) consisting of eight treatment combinations with four replications and contained 32 experimental units. The treatments used were clean and jambul/ajir pruning, 40 cm and 60 cm pruning height and BAP concentration. The variable observed were number of pecco shoots, number of banji shoots, fresh weight and dry weight of shoots. The results showed that the combination of type and height of pruning with the BAP application affected the number of pecco and banji shoots on the third picking, but did not affect the fresh and dry weight of shoots. The treatment of clean and jambul/ajir pruning at 60 cm height and added with BAP application at 60 ppm exhibited the best treatment in producing the number of shoots and reduce the number of banji shoots.&lt;/em&gt;