The angioarchitecture of 9, 10-dimethyl-1, 2-benzanthracene (DNIBA)-induced carcinoma of the tongue in hamster was examined mainly by resin cast method using specimens at 5 stages (initial, diffuse growth, minute granu.liform elevation/small buldging, papilloma and cancer stages) with special reference to angiogenesis. Innervation of the vessels was also examined by catecholamine (CA) fluorescence, acetylcholine esterase (AChE), and horseradish peroxidase (HRP) histochemical methods. The results obtained were as follows:1. From the initial to the diffuse growth stage, elongation and enlargement of capillary loops in the tunica propria mucosa became visible.2. After the minute granuliform elevation/small buldging stage, characteristic capillary loops were often observed in the resin cast preparations suggesting a possible participation of budding and fusion in the angiogenesis.3. At the papilloma stage, the resin cast of the vasculature revealed a characteristic tree-like constitution having extremely swollen tips. Cross sections of the vessels also revealed their unique wall structures, as swollen and thin as sinusoidal vessels.4. At the carcinoma stage, extremely flattened or shrunken vessels were predominant, however some buddings were also found suggesting a continuous involvement of angiogenesis.5. At the external growth part from the initial to the papilloma stage, the tunica propria mucosa frequently exhibited an apparent increase in thickness. Both the HRP and the CA positive nerves were observed in the thickened mucosa. These nerves, however, were not seen at the cancer stage.6. In the musculature infiltrated by cancer cells, both the CA and the AChE positive nerves innervating the blood vessels tended to degenerate and disappear. The HRP positive nerves also showed a degeneration due to cancer infiltration, although it remained unclear whether they were related to the vessels or not.7. The budding, elongation, enlargement, and fusion of capillary loops in the tunica mucosa may play a role in the rapid formation of the characteristic vascular architecture of DMBA-induced carcinoma of the tongue.8. The tumor blood vessels, whose walls were very thin and were mainly composed of endothelial cells, may be suitable for the rapid increase in the vessel volume. Because of such characteristic features, the tumor blood vessels may easily flatten, close their lumens, and regress under high tissue pressure at the cancer stage. These structural alterations in blood vessels may finally result in degeneration of the tumor.9. The paucity of musculature and innervation in the tumor blood vessels suggests that the vessels serve the tumor as the exchange vessels rather than the distributing vessels. The tumor blood circulation, therefore, seems to depend on the control mechanism of the external vessels of the tumor.