Apical Dentin Research Articles

Exploring the Effects of Different Torque and Speed Parameters on Smear Layer and Microcrack Formation in Apical Dentin of Root Canals with Single File Instrumentation

Exploring the Effects of Different Torque and Speed Parameters on Smear Layer and Microcrack Formation in Apical Dentin of Root Canals with Single File Instrumentation

Regenerative endodontic therapy after autogenous transplantation of mandibular third molar

Autogenous tooth transplantation involves moving a tooth from its original position to replace a missing tooth. Complications, such as pulp necrosis, can occur following autotransplantation of teeth with incomplete root formation. There are insufficient studies on managing pulp necrosis in autotransplanted immature teeth by regenerative endodontic treatment (RET). In this case, a 21-year-old woman developed a sinus tract 3 months after autotransplantation of an immature third molar that replaced the lower left mandibular first molar. She presented with pain while chewing in the area of her autografted third molar. Based on clinical findings and periapical radiolucency in radiographic findings, the tooth was diagnosed with necrotic pulp and apical periodontitis. It was decided to perform RET for the tooth. The tooth underwent chemomechanical debridement and calcium hydroxide dressing in the first session. After 2 weeks, the patient’s pain and sinus tract were resolved. In the second session, the periapical tissue was stimulated by inserting a K-file 2 mm over the apex to induce bleeding in the root canal. Mineral trioxide aggregate was placed over the blood clot, and the tooth received final restoration. After 11 months of recall appointments, the apical foramen size had decreased the thickness of the apical dentin wall increased, there was no periapical pathosis, and the periodontal ligament space width was normal. This case report provides valuable insights into the pulpal management of autotransplanted immature teeth.

Efficacy of Smear Layer Removal at the Apical One-Third of the Root Using Different Protocols of Erbium-Doped Yttrium Aluminium Garnet (Er:YAG) Laser.

Background and Objectives: Smear layer forms during cleaning and shaping can obstruct the entry of both irrigant and sealant into the dentinal tubules, resulting in the accumulation of the bacteria and their byproducts. To ensure effective adhesion and better periapical healing, it is strongly advised to remove the smear layer before proceeding with root canal obturation. This study was designed to compare the efficiency of laser-activated irrigation (LAI) in removing the smear layer and debriding the most apical third of the root canal. Materials and Methods: Sixty-five extracted human teeth with straight single roots were randomly and equally divided into four laser and one control groups. Root canals in all samples were shaped using prime size TruNatomy rotary files. During preparation, each canal was irrigated with 3 mL of 3% NaOCl and 3 mL of 17% EDTA alternately, followed by the irrigation with 10 mL of distilled water to avoid the prolonged effect of EDTA and NaOCl solutions. Final irrigation of 5 mL of 17% EDTA of the root canal was done to eliminate the smear layer and was subsequently activated by an endodontic ultrasonic tip for 20 s three times (control group), a flat-end laser tip (test groups 1 and 3) or a taper-end laser tip (test groups 2 and 4) for two cycles. The time of each cycle activation was 10 s (groups 1 and 2) or 20 s (groups 3 and 4) in which the Er:YAG laser of 2940 nm was used. The laser operating parameters were 15 Hz and 50 μs pulse duration. The samples were then split longitudinally and subjected to scanning electron microscopy (SEM). Results: The remaining smear layer at the apical part of the root canals was statistically significant between the control group and the laser groups 1 (p = 0.040) and 2 (p = 0.000). Within the laser groups, the exposed tubules count was greater in the laser with the flat tip as compared with the tapered tip (Laser 1 > Laser 2 and Laser 3 > Laser 4). Finally, no significant differences in the count of debris between the laser groups and control group were observed, except for laser 4 (p < 0.05), which had the highest count of debris. Conclusion: LAI to remove debris and smear layer at the apical third of the root canal is inferior to the current ultrasonic technique. However, when using the Er:YAG LAI, it is recommended to use a flat tip design for 10 s for two cycles to ensure maximum debridement of the apical dentin surface.

Comparative Evaluation of Retention of Fiber Posts in Different Dentin Regions Using Various Bonding Techniques: An In Vitro Study.

Aim This study aims to evaluate the retention of fiber posts in the coronal, middle, and apical dentin regions with various bonding techniques by using fifth, sixth, and eighth-generation bonding agents and self-etch adhesive cement. Materials and methods For this study, 96 freshly extracted human incisors with straight roots were selected. Endodontic treatment of the specimens was performed. The post spaces were created immediately after obturation and the posts were luted with three different bonding agents and self-adhesive resin cement and the specimens were divided into four groups. Approximately 3 mm thick sections were made from different dentin regions of the post space and were tested for three subgroups: Subgroup I: Coronal, Subgroup II: Middle, Subgroup III: Apical. The specimens were tested on a universal testing machine. Results Statistical analysis was done using one-way analysis of variance (ANOVA) and Tukey's post hoc test usingSPSS software version 17.0 (SPSS Inc., Chicago ). The intra-group comparison showed that the bond strength was more in the fifth-generation bonding agent (Cervical- 8.2574± 1.49034, Middle- 11.4800± 2.59938, Apical- 14.7108±1.82931), followed by the sixth (Cervical- 9.102± 2.76119, Middle-9.3152±2.11585, Apical- 12.9478±4.69404) and eighth-generation bonding systems (Cervical- 9.0938±2.77537, Middle- 7.1585±1.97601, Apical- 9.3726±0.73720) and the self-etch adhesive dental resin cement (Cervical- 5.1004±2.17389, Middle- 4.1574±1.28664, Apical-7.8884±1.90078). The inter-group comparison showed that the bond strength was higher in the fifth-generation bonding agent followed bysixth-generation, eighth-generation, and self-adhesive resin cement. Conclusion The present study reveals that the highest push-out bond strengths were obtained in apical sections followed by the middle and cervical areas. Thebond strength was higher when using the fifth-generation bonding agent followed by the sixth, eighth, and self-adhesive resin cement. Despite many advancements, the fifth-generation bonding agentstill showed superior retention in different dentin regions among various other bonding techniques.

Influence of alveolar bone height on the biomechanical behavior of roots restored with custom-made posts-and-cores.

This study evaluated the influence of alveolar bone height and post type on compressive force resistance, fracture pattern, and stress distribution in endodontically treated teeth. Bovine roots were endodontically treated and divided into eight groups (n=10) according to alveolar bone height (normal alveolar bone and alveolar bone loss - 2 and 5 mm from the margin of the crown, respectively) and post type (prefabricated glass fiber post, anatomic glass fiber post, customized milled glass fiber post-and-core and customized milled polyetheretherketone (PEEK) post-and-core). Mechanical fatigue was simulated (300.000 cycles/50 N/1.2Hz). Compression force resistance (N) was analyzed by two-way ANOVA and Tukey test (α=0.05). Fracture patterns were described as percentages. Stress distribution was analyzed by finite element analysis. Significant diferences were found for alveolar bone height (P < 0.0001): normal alveolar bone groups showed higher mean values of compression force resistance compared to alveolar bone loss groups, while no significant differences were found for post type (P=0.4551), and there was no double interaction between them (P=0.5837). Reparable fractures were more predominant in normal alveolar bone groups, especially in the milled glass fiber and PEEK post-and-core groups. Stress distribution was similar in groups with prefabricated glass fiber posts and milled PEEK posts-and-cores, and the alveolar bone loss condition significantly increased stress concentration and strain values, mainly on apical dentin. Alveolar bone loss due to physiological aging and/or periodontal disease may lead to increased risk of restored tooth failure, although milled glass fiber and PEEK posts-and-cores provide more reparable fractures. Custom-made glass fiber and PEEK post-and-cores are interesting options, since they enable clinicians to work with a single-body post-and-core system that avoid several materials interfaces and fits well in the root canal provided promising results to improve the failure behavior of restored roots, as they offer more reparable fractures even in situations of alveolar bone loss.

Minimum residual root dentin thickness of mandibular premolars restored with a post: A finite element analysis study

Statement of problemThin root dentin after post space preparation will increase the risk of root fracture. However, the minimum residual root dentin thickness to be preserved after post space preparation is unclear. PurposeThe purpose of this finite element analysis (FEA) study was to measure the residual root dentin thicknesses and analyze the stress distributions of post-restored mandibular premolars. Material and methodsThe cone beam computed tomography (CBCT) data of 90 first and second mandibular premolars from Chinese participants (44 men, 46 women; aged between 20 and 79 years) were analyzed. Cross-sections 5 to 9 mm from the radiologic apex were used to measure the buccolingual and mesiodistal root diameters. The probability that the residual thickness of the buccolingual and mesiodistal root walls would be no less than 0.5, 0.6, 0.7, 0.8, 0.9, and 1 mm after post space preparation with a #2 Peeso reamer was calculated. Six 3-dimensional finite element models of cast post-restored mandibular premolars with 0.5, 0.6, 0.7, 0.8, 0.9, and 1 mm mesial and distal root dentin thicknesses were established. A static force of 100 N was applied to the buccal cusp tip at 45, 60, 75, and 90 degrees to the long axis of the tooth, and the maximum tensile stress and von Mises stress were analyzed. The Bonferroni post hoc test (αcorrected=.003) was used for multiple comparisons. ResultsThe buccolingual root diameter of mandibular premolars was wider than the mesiodistal root diameter. The probabilities that the mesiodistal residual root dentin thickness of mandibular first and second premolars at 5 mm from the apex would be no less than 1 mm after post space preparation with a #2 Peeso reamer were only 10% and 28%, respectively. The maximum tensile stress was at a minimum when the mesial and distal residual root dentin thickness was 0.6 mm in the external cervical dentin adjacent to the crestal bone and 1 mm in the apical dentin corresponding to the apex of the post. The maximum tensile stress of the mandibular premolar model with 0.6-mm mesial and distal residual root dentin thicknesses was lower than that with 0.9 mm and 1.0 mm thicknesses (Pcorrected<.001). ConclusionsShort posts or smaller instruments for post space preparation are recommended to obtain a 1-mm residual root dentin thickness in the mesiodistal direction of mandibular premolars. If a 1-mm thickness cannot be preserved, a minimum residual root dentin thickness of 0.6 to 0.9 mm in the mesiodistal direction should be retained.

Quantitative Analysis of Cone-Beam Computed Tomography Artifacts Induced by Nonmetallic Root Canal Filling Materials Using Different Fields of View: In Vitro Study.

Cone-beam computed tomography (CBCT) imaging artifacts can hinder accurate diagnosis of several conditions. The aim of this study was to quantify CBCT artifacts created by nonmetallic root canal filling materials using two fields of view (FOV). Root canals of twenty extracted maxillary central incisors (n = 20) were instrumented and randomly divided into four equal groups: canals in Group 1 were filled with gutta-percha, canals in Group 2 with mineral trioxide aggregate (MTA) and gutta-percha, canals in Group 3 with gutta-percha and glass-fiber posts, and canals in Group 4 with MTA and glass-fiber posts. Each tooth was mounted on a silicon block and scanned three times using a CBCT machine, first with a prefilling scan (control) and then with postfilling scans, using two different FOV (40∗50 mm and 80∗50 mm). Imaging software was used to measure grayscale pixel values of canal cross sections. Data were analyzed using t-test and paired t-test statistical tests, with statistical significance set at p ≤ 0.05. Dentin at the apical and middle third of the teeth showed a significant decrease in minimum grayscale values in association with gutta-percha (p = 0.027, p = 0.034). However, a wide 80∗50 FOV showed a significant increase in maximum grayscale values of coronal (p = 0.048) and apical dentin (p = 0.049). Glass-fiber posts in middle third cross sections also corresponded to an increase in maximum grayscale values (p = 0.018). Gutta-percha produced dark band artifacts in the root's middle and apical thirds, whereas white streak artifacts were produced by gutta-percha in the coronal third and glass-fiber posts in the coronal and middle thirds when imaged with a wider FOV. A smaller FOV is therefore recommended for CBCT imaging, as it produces fewer artifacts.

Dentin Disinfection Efficacy Using Four Different Irrigation Protocols

Objective: The objective of this study was to observe the effect that 3% Sodium Hypochlorite and 17% Ethylenediamine Tetraacetic Acid (EDTA) with water in between, used sequentially and individually had in the disinfection of dentin blocks that were contaminated with Enterococcus faecalis. Materials and Methods: Thirty apical and coronal dentin block samples were divided into five groups (n=5): All samples were inoculated with Enterococcus faecalis Group 1: Samples were submerged in 3% NaOCl then in 17% EDTA. Group 2: Samples were submerged first in 17% EDTA and then in 3% NaOCl. Group 3: Samples were immersed in 3% NaOCl only. Group 4: Samples were submerged in 17% EDTA only. Group 5 (positive control group): Samples were only submerged in distilled water. All samples were submerged in distilled water after each irrigation cycle. The irrigation protocol was repeated in all groups until all dentin samples were exposed to 24ml of the irrigating solutions. CFU units were counted and classified in an ordinal scale and compared with the linear-by-linear association test. Results: A significant linear trend in the reduction of CFU was observed when NaOCl and EDTA were combined (independently of the sequence used) when compared to groups 3 and 4 where the irrigants were used individually both in coronal (p=9.45 x 10-21), and apical slices (p=2.33 x 10-20). NaOCl was significantly more effective than EDTA in both coronal and apical slices (p ≥ 0.000001) when used alone. Conclusion: 3% Sodium Hypochlorite was more effective than 17% EDTA. However, alternating 3% NaOCl with 17% EDTA resulted in better dentinal disinfection. Clinical Relevance: Establishing an irrigation protocol that is effective in eradicating bacteria entrenched in dentinal tubules can lead to a more successful outcome in endodontically treated teeth.

Melatonin-doped polymeric nanoparticles induce high crystalline apatite formation in root dentin

ObjectiveTo investigate the effect of novel polymeric nanoparticles (NPs) doped with melatonin (ML) on nano-hardness, crystallinity and ultrastructure of the formed hydroxyapatite after endodontic treatment. MethodsUndoped-NPs and ML-doped NPs (ML-NPs) were tested at radicular dentin, after 24 h and 6 m. A control group without NPs was included. Radicular cervical and apical dentin surfaces were studied by nano-hardness measurements, X-ray diffraction and transmission electron microscopy. Mean and standard deviation were analyzed by ANOVA and Student-Newman-Keuls multiple comparisons (p < 0.05). ResultsCervical dentin treated with undoped NPs maintained its nano-hardness values after 6 m of storage being [24 h: 0.29 (0.01); 6 m: 0.30 (0.02) GPa], but it decreased at apical dentin [24 h: 0.36 (0.01); 6 m: 0.28 (0.02) GPa]. When ML-NPs were used, nano-hardness was similar over time [24h: 0.31 (0.02); 6 m: 0.28 (0.03) GPa], at apical dentin. Root dentin treated with ML-NPs produced, in general, high crystallinity of new minerals and thicker crystals than those produced in the rest of the groups. After 6 m, crystals became organized in randomly oriented polyhedral, square polygonal block-like apatite or drop-like apatite polycrystalline lattices when ML-NPs were used. Undoped NPs generated poor crystallinity, with preferred orientation of small crystallite and increased microstrain. SignificanceNew polycrystalline formations encountered in dentin treated with ML-NPs may produce structural dentin stability and high mechanical performance at the root. The decrease of mechanical properties over time in dentin treated without NPs indicates scarce remineralization potential, dentin demineralization and further potential degradation. The amorphous stage may provide high hydroxyapatite solubility and remineralizing activity.

Analysis of Instrumentation Protocols Regarding the Quality of Mesial Canal Preparation in Mandibular Molars: A Micro–computed Tomographic Study

IntroductionThis study aimed to use micro–computed tomographic imaging to analyze the quality of the endodontic preparation of mesial canals in mandibular molars provided by 3 instrumentation protocols. MethodsForty-five extracted mandibular molars with 2 independent mesial canals were selected, and the initial micro–computed tomographic imaging was performed. The initial volume values of the canals were submitted to statistical analysis for paired division. The groups were determined according to the final enlargement of the canal and the working length adopted (ie, G25.06/+1 mm, G35.05/foramen, and G50.01/−1 mm). At the end of each instrumentation sequence, the root canals were scanned and analyzed with regard to the increase in the total and apical volume, centralization, and preparation transportation and the percentage of the total and apical uninstrumented walls. ResultsFor the intragroup comparison, the Wilcoxon test was used, and for the intergroup analysis, the Kruskal-Wallis and Dunn tests were used (P < .05). In the analysis of the canal total volume, a statistical difference was found between G25.06/+1 mm and the remaining groups (P < .05). In the apical third, a statistical difference was observed between G25.06/+1 mm and G50.01/−1 mm (P < .05). No statistical difference was found between the groups in terms of centralization and transportation of the preparation or in terms of the percentage of the total or apical uninstrumented walls. ConclusionsThe preparation of the mesial canals of mandibular molars up to larger tip files but with a lower taper at 1 mm before the foramen resulted in a larger volume of apical preparation, kept the preparation centralized, and provided safe apical dentin wear without excessive cervical wear.

Dental Characteristics of Microcephalic Osteodysplastic Primordial Dwarfism Type II

Microcephalic osteodysplastic primordial dwarfism type II (MOPD II) is an autosomal recessive inherited disorder form of primordial dwarfism, caused by mutations in the pericentrin gene. The purpose of the study was to examine the clinical and radiological features, physicochemical properties and microstructures of the tooth affected with MOPD II. The mandibular 2nd molar was collected from the MOPD II patient. Micro-computerized tomography, scanning electron microscopy, energy dispersive spectrometry and Vickers microhardness analysis were performed on the MOPD II and the normal control. The morphology of the MOPD II tooth appeared to have malformed pulp and root and showed a small size. The mineral density measurement showed that the MOPD II tooth had similar scores in the enamel, but lower scores in the root 1/2 and apical dentin compared to the normal control. The microhardness values were smaller in the cusp enamel, root 1/2 dentin and apical dentin of the MOPD II compared to the normal control. In this study, the dental characteristics and the physicochemical properties of a tooth affected with MOPD II were analyzed to improve understanding of the oral manifestations of the disease and to assist in proper dental treatment by identifying precautions.

Polymeric nanoparticles for endodontic therapy

The effectiveness of novel polymeric nanoparticles (NPs) application in reducing dentin permeability and facilitating dentin remineralization after endodontic treatment was evaluated. The effect of undoped NPs, zinc, calcium and doxycycline-doped NPs (Zn-NPs, Ca-NPs and D-NPs, respectively) was tested in radicular dentin. A control group without NPs was included. Radicular dentin was assessed for fluid filtration. Dentin remineralization was analyzed by scanning and transmission electron microscopy, energy-dispersive analysis, AFM, Young's modulus (Ei), Nano DMA, Raman, and X-Ray Diffraction analysis. Ca-NPs and Zn-NPs treated dentin exhibited the lowest microleakage with hermetically sealed dentinal tubules and a zinc-based salt generation onto dentin. Zn-NPs favored crystallinity and promoted the highest Ei and functional remineralization at the apical dentin, generating differences between the values of complex modulus among groups. Ca-NPs produced closure of tubules and porosities at the expense of a relative mineral amorphization, without creating zones of stress concentration. The highest sealing efficacy was obtained in Zn-NPs-treated samples, along with the highest values of Young's modulus and dentin mineralization. These high values of Ei were obtained by closing voids, cracks, pores and tubules, and by strengthening the root dentin. When using undoped NPs or Ca-NPs, deposition of minerals occurred, but radicular dentin was not mechanically reinforced. Therefore, application of Zn-NPs in endodontically treated teeth previous to the canal filling is encouraged.

Hydroxyapatite-based cements induce different apatite formation in radicular dentin

ObjectiveTo investigate crystallinity and ultrastructure of the formed hydroxyapatite at radicular cervical and apical dentin after being treated with three different canal sealers. MethodsCervical and apical root dentin surfaces were treated with two experimental hydroxyapatite-based sealers, containing sodium hydroxide (calcypatite) or zinc oxide (oxipatite) and an epoxy resin-based canal sealer (AH Plus); gutta-percha without sealer was included as control. Dentin surfaces were studied by X-ray diffraction and transmission electron microscopy through selected area diffraction and bright-field imaging after 24h and 12m of storage. ResultsRoot cervical dentin treated with calcypatite and oxipatite produced poor crystallinity of new minerals, wide amorphous phase and non-stoichiometry. Reflections at the 002 plane and the corresponding diffraction rings attained lower values in the Scherrer equation and the Scherrer-Wilson equation in samples treated with both HAp-based sealers than in specimens without sealer or with AH Plus. At root cervical dentin treated with calcypatite, shorter and wider crystallite size formations and lower crystals grain size were found, if compared to those encountered at oxipatite treated dentin. Oxipatite attained improved crystallographic atomic order and less structural variation in both distances and angles. Apical dentin treated with oxipatite attained preferred grain orientation with polycrystalline lattices. SignificanceThe immature crystallites formed in dentin treated with calcypatite and oxipatite will account for high hydroxyapatite solubility and remineralizing activity. New polycrystalline formations encountered in apical dentin treated with oxipatite may also produce high mechanical performance.

A zinc oxide-modified hydroxyapatite-based cement favored sealing ability in endodontically treated teeth

ObjectivesTo evaluate the effectiveness of different endodontic canal sealers for dentin permeability reduction and to determine the viscoelastic performance of root dentin after their application. MethodsCervical, medial and apical root dentin surfaces were treated with two experimental hydroxyapatite-based cements, containing sodium hydroxide (calcypatite) or zinc oxide (oxipatite); an epoxy resin- based canal sealer, AH Plus; and gutta-percha. Root dentin was evaluated for fluid filtration. Field emission scanning electron microscopy, energy dispersive analysis, AFM, Young’s modulus and Nano-DMA analysis were also performed, at the inner and outer zones of dentin. ResultsDentin treated with oxipatite showed the lowest microleakage among groups with hermetically sealed tubules and zinc-based salt formations. Samples treated with oxipatite showed the highest Ei at the cervical dentin third among groups, at 6 m of storage. Oxipatite promoted the highest complex modulus and tan delta values at the inner zone of both cervical and medial root dentin. Calcypatite favored the lowest tan delta outcomes at the inner zone of apical dentin at 6 m. ConclusionsSpecimens treated with oxipatite showed the highest sealing ability, based on the highest Young’s modulus and dentin mineralization, achieved by closing dentinal tubules, voids and pores that reinforced the inner zone of root dentin. The homogeneity of viscoelastic properties among the different root dentin thirds favored the energy dissipation without creating zones of stress concentration and micro-cracking which would have challenge micropermeability. Thereby, among the tested materials oxipatite is proposed as canal filling material and sealer in endodontics. Clinical significanceOxipatite could be considered a good candidate for root canal filling material and sealer due to its improved long-term sealing ability and to the advanced remineralization, and so to the enhanced energy dissipation achieved at the inner zone of the radicular dentin.

Free Water Loss–induced Heterogeneous Residual Strain and Reduced Fatigue Resistance in Root Dentin: A 3-dimensional Digital Image Correlation Analysis

IntroductionThis study evaluated free water loss–induced residual strain with and without axial compressive loading and assessed the mechanical effect of cyclic loading in fully hydrated and partially dehydrated root dentin. MethodsRoot dentin sections prepared from freshly extracted human premolars were used. Customized 3-dimensional digital image correlation was used to qualitatively and quantitatively analyze the residual strain induced by 2 hours of free water loss in different regions of root dentin. Residual strain in partially dehydrated root dentin during axial compressive loading was also analyzed using 3-dimensional digital image correlation. The effect of cyclic loading on load to fracture in fully hydrated and partially dehydrated dentin and their fractography were analyzed using micro–computed tomographic imaging. ResultsFree water loss resulted in a heterogeneous distribution of residual strain and an overall formation of residual compressive strain with areas of tensile strain localized to the root canal and outer dentin. More residual compressive strain was observed in the apical dentin compared with the cervical dentin (P < .05), and more residual shear strain was observed in outer dentin compared with inner dentin (P < .05). Axial loading resulted in an increase in the load-induced compressive strain in the direction perpendicular to dentinal tubules (P < .05). Fully hydrated roots displayed a higher mean (P < .05) and median (P < .05) number of cycles to fracture with microcracks characteristic of toughness. ConclusionsAfter free water loss, root dentin displayed an increased formation of heterogenous residual strain, which resulted in increased axial compressive load-induced strain and a decreased resistance to fatigue failure. The effect of free water loss in the loss of mechanical integrity of root-filled teeth needs further investigation.

Ascertaining of age by Raman spectroscopic analysis of apical dentin - A forensic study.

Context:Age estimation in forensics employ various methods of which Raman microspectroscopy provides a noninvasive method by assessing various parts of dentin.Aim and Objectives:The aim of this study is to ascertain the age of carious tooth using Raman spectra of apical dentin and to correlate the similarity of the spectra in a defined age group.Settings and Design:Teeth of known age from Indian population (n=48) and morphology (incisors, canine, premolars, molars) indicated for extraction due to caries were allocated into four groups, i.e., Group A (21-30 years), Group B (31-40 years), Group C (41-50 years) and Group D (51-60 years).Materials and Methods:Teeth were sectioned and the apical 2 mm of dentin was examined by a Raman microspectroscopy machine for spectra from 400 cm−1 to 1500 cm−1, and peak at phosphate group at 963 cm−1 was taken for statistical analysis.Statistical Analysis Used:The data were analyzed using IBM SPSS version 20.0.Results:Pearson's correlation to test the strength of linear relation of the spectra of the teeth within an age group showed an “r” value approaching 1. One-way ANOVA to test the difference between means of spectrum values obtained between the four age groups was statistically significant at P < 0.05.Conclusion:Raman spectroscopic analysis of apical dentin of teeth affected by caries can also be used to estimate the age and the Raman spectra obtained differed for different age groups, and the same can be advocated as an alternative method to ascertain age in forensic dentistry.

Comparative evaluation of the pushout bond strength of two root-end materials: An in vitro study.

Aim:The aim of this study is to evaluate the pushout bond strength of two bioceramic materials, two component calcium trisilicate material (biodentine), and premixed calcium trisilicate putty (Endosequence root repair material-fast set putty).Subjects and Methods:20 maxillary incisors were used in the study. Apical sections of 3mm were obtained and retro cavities to a depth of 3mm were prepared using a straight fissure bur. Another transverse section was made 4-mm coronal to the previous section. The specimens were placed into acrylic resin rings and separated into two groups and their cavities filled with the materials. The pushout test was carried out using a universal testing machine at a cross head speed of 0.5 mm/min, and the specimens were examined in a stereomicroscope at to evaluate the modes of failure.Statistical Analysis:The pushout bond strength values were analyzed using the unpaired t-test, and the modes of failure were compared using Fisher's exact test.Results:The pushout bond strength was seen to be significantly higher (P < 0.001) for the two-component calcium trisilicate material (11.596 ± 3.309). Cohesive failure patterns were observed in both the test groups.Conclusion:Two component calcium trisilicate material demonstrated higher bond strength values to apical dentine.

Molecular expression of Mg2+ regulator TRPM7 and CNNM4 in rat odontoblasts

ObjectiveMagnesium, the second most abundant cation in cellular fluid, is critical for mineralization of hard tissues. Among the molecules involved in cellular Mg2+ homeostasis, functional impairment of Mg2+ permeable ion channel TRPM7 or Mg2+ transporter CNNM4 have been found to result in severe hypomineralization of the enamel and dentin. However, molecular expressions of TRPM7, CNNM4 and their respective homologues have not been fully investigated in adult odontoblasts. DesignExpressions of TRPM6, TRPM7, CNNM1, CNNM2, CNNM3, CNNM4 were screened in acutely dissociated rat odontoblasts by single cell RT-PCR. Among these candidates, expression levels of TRPM7 and CNNM4 were compared along the odontoblast layer by immunohistochemical analysis. Finally, the coexpression pattern of TRPM7 and CNNM4 in subcellular regions was examined by immunocytochemical analysis. ResultsScRT-PCR revealed high expression rate of TRPM7 and CNNM4 in odontoblasts, with CNNM4 detected almost exclusively in TRPM7-positive odontoblasts. However, CNNM2 and CNNM3 were detected in only a small population of odontoblasts, and TRPM6 and CNNM1 were not detected even in the pulp tissue. Immunohistochemical analysis revealed higher CNNM4 expression in the apical odontoblast layer than the coronal area, in contrast to the ubiquitous expression of TRPM7. Lastly, immunocytochemical analysis revealed colocalization of CNNM4 with TRPM7 in the odontoblastic process. ConclusionsCNNM4 and TRPM7 may serve as main Mg2+ regulators in odontoblasts, possibly with selective involvement of CNNM4 in apical dentin formation or mineralization. Colocalization of TRPM7 and CNNM4 in the odontoblastic process suggest functional coupling of these two molecules to maintain Mg2+ homeostasis.

Influence of irrigating agents on fiber postpush-out bond strength to radicular dentin sections with the different adhesive system.

Objective:The objective of this study is to evaluate the effect of irrigating agents on push-out bond strength of resin postcemented with various adhesive systems at different radicular dentin sections.Materials and Methods:Sixty single-rooted premolar teeth were root canal treated, subsequently decorated at cementoenamel junction. The endodontic postspace was irrigated with 5.25% sodium hypochlorite (NaOCl) and 17% ethylenediaminetetraacetic acid (EDTA) for Group A (n = 30) and Group B (n = 30), respectively. The sample from each group was subdivided into three groups (10) according to luting protocol of etch-wash, self-etch, and self-adhesive. Individual teeth with cemented resin postsamples were sectioned into coronal, middle, and apical segments. Subsequently, subjected for pushout bond strength test by applying a load at 0.5 mm/min speed. The data were analyzed by three-way analysis of variance and Tukey comparison test at a significant difference level of 0.05.Results:The coronal section with 5.25% NaOCl irrigation and self-etch luting protocol provided the highest push out strength at 16.282 Mpa. The etch-wash luting protocol in both irrigations showed the lesser bond strength at 8.273 and 8.493 MPa, respectively, in coronal section.Conclusions:The self-etch adhesive system showed better push out bond strength and 17% EDTA had a negative influence on self-etch bond strength. The coronal sections had highest bond strength in comparison with apical radicular dentin sections.

Physicochemical Properties and Dentin Bond Strength of a Tricalcium Silicate-Based Retrograde Material.

The aim of this study was to evaluate the physicochemical properties and the apical dentin bond strength of the tricalcium silicate-based Biodentine in comparison to white MTA and zinc oxide eugenol-based cement (ZOE). Setting time and radiopacity were evaluated according to ISO 6876:2012 specification. Final setting time, compressive strength and pH were also assessed. Material's bond strength to the apical root canal dentin was measured by the push-out assay. Data were analyzed by ANOVA and Tukey-Krammer post-hoc test. Biodentine presented the shortest initial (16.2±1.48 min) and final setting time (35.4±5.55 min). Radiopacity of Biodentine (2.79±0.27 mmAl) does not agree with ISO 6876:2012 specifications. On the other hand, Biodentine showed higher compressive strength after 21 days (37.22±5.27 MPa) and higher dentin bond strength (11.2±2.16 MPa) in comparison to white MTA (27.68±3.56 MPa for compressive strength and 2.98±0.64 MPa for bond strength) (p<0.05). Both MTA and Biodentine produced an alkaline environment (approximately pH 10) (p>0.05) compared to ZOE (pH 7). It may be concluded that Biodentine exhibited faster setting, higher long-term compressive strength and bond strength to the apical dentin than MTA and ZOE.