Welding is used as the main joining method in various industries, including the shipbuilding industry. In the case of welded structures, structural integrity assessment is essential to ensure the safety of the structure because many defects inevitably exist during the manufacturing process. The value of reliable fracture toughness is required for structural integrity assessment. It is obtained by the fracture toughness test, but the fracture toughness test requires a lot of time and effort. Therefore, many studies have been conducted on efficient methods to evaluate fracture toughness. Among the various studies that estimate fracture toughness, some have been conducted using the Charpy impact test, which is relatively simple compared to the fracture toughness test. This study conducted a series of experimental investigations on API 2W Gr.50 steel applied with different welding conditions. Based on the Charpy impact test results, the fracture behavior was well estimated in the ductile to brittle transition region according to the temperature. However, there was a difference in the accuracy of predicting fracture behavior depending on the welding process. Therefore, additional consideration reflecting the various welding conditions is required to ensure the safety of welded structures.