Aperture Modulation Research Articles

Split-Aperture 2-in-1 Computational Cameras

While conventional cameras offer versatility for applications ranging from amateur photography to autonomous driving, computational cameras allow for domain-specific adaption. Cameras with co-designed optics and image processing algorithms enable high-dynamic-range image recovery, depth estimation, and hyperspectral imaging through optically encoding scene information that is otherwise undetected by conventional cameras. However, this optical encoding creates a challenging inverse reconstruction problem for conventional image recovery, and often lowers the overall photographic quality. Thus computational cameras with domain-specific optics have only been adopted in a few specialized applications where the captured information cannot be acquired in other ways. In this work, we investigate a method that combines two optical systems into one to tackle this challenge. We split the aperture of a conventional camera into two halves: one which applies an application-specific modulation to the incident light via a diffractive optical element to produce a coded image capture, and one which applies no modulation to produce a conventional image capture. Co-designing the phase modulation of the split aperture with a dual-pixel sensor allows us to simultaneously capture these coded and uncoded images without increasing physical or computational footprint. With an uncoded conventional image alongside the optically coded image in hand, we investigate image reconstruction methods that are conditioned on the conventional image, making it possible to eliminate artifacts and compute costs that existing methods struggle with. We assess the proposed method with 2-in-1 cameras for optical high-dynamic-range reconstruction, monocular depth estimation, and hyperspectral imaging, comparing favorably to all tested methods in all applications.

Simple implementation of aperture modulation quantitative differential phase contrast imaging

As one of the quantitative phase imaging methods, aperture modulation quantitative differential phase contrast (amqDPC) imaging has isotropic lateral resolution, being widely used in many fields such as biological cell morphology detection. However, the devices required to implement it are poorly controlled and very expensive. In addition, due to the weak phase approximation, there is a large axial phase error, and the full spectrum center pass during aperture modulation will cause distortion. Therefore, a method that enables simple and low-cost implementation of amqDPC, high axial phase accuracy, and isotopic lateral resolution is reported. Through simulations and experimental verifications, axial accuracy is greatly improved in the phase range of [0, π], and an isotropic lateral resolution of 870 nm can be achieved. In addition, experiments on transparent glass circular step sample and mouse kidney tissue slice also prove the practicability of this method. According to this study, researchers can accurately and simply measure the axial height of a slightly thick sample, which is achieved by capturing two intensity images to reconstruct a quantitative phase.

Programmable Aperture Light‐Field Microscopy

AbstractA computational three‐dimensional (3D) microscopy technique, termed programmable aperture light‐field microscopy (PALFM), for motion‐free, high‐resolution volumetric imaging of fluorescent or self‐luminous samples is proposed. The well‐known Fourier slice theorem is extended to incoherent tomographic imaging, which states that a detected image under an ideal aperture corresponds to a central slice in the 3D object spectrum, so the spectrum coverage can be accomplished based on the motion‐free aperture modulation. When further considering frequency extension and coverage, a hybrid aperture modulation scheme is designed consisting of non‐centrosymmetric circular and annular apertures for high‐efficiency, non‐ambiguous depth discrimination. A PALFM system with an easy‐to‐build programmable aperture module attached to an off‐the‐shelf inverted fluorescence microscope is constructed, where annular apertures can manipulate Bessel‐like beams for spectrum modulation. Experimental results on near‐diffraction‐limited imaging of a resolution target across a large depth range and high‐resolution, multi‐color 3D imaging of a mouse kidney section verify the validity and effectiveness of PALFM. High‐speed, long‐term time‐lapse volumetric imaging of HeLa cells in vitro further demonstrates that PALFM is a promising tomographic imaging tool for studying dynamic cellular processes and events without requiring complicated sample rotation or beam scanning.

A reusable and leakage-proof immobilized laccase@UiO-66-NH2(30) for the efficient biodegradation of rifampicin and lincomycin

The enzyme leakage in physical adsorption dramatically limits the application of immobilized laccase (Lac) in environmental treatment. Therefore, a leakage-proof Zr-based metal-organic framework (MOF) carrier, UiO-66-NH2(30), was constructed for the stable loading of Lac and efficient antibiotic biodegradation, which could fix the Lac inside the carrier rather than only adsorbed on the surface. Based on the aperture modulation strategy, the pore size distribution of MOFs at about 10 nm was significantly increased to match the diameter of Lac (7.6 nm), by which Lac@UiO-66-NH2(30) was successfully prepared with a high enzyme loading amount (275.96 mg/g) and high enzyme recovery (618 %). The immobilized Lac@UiO-66-NH2(30) could nearly 100 % biodegrade rifampicin and lincomycin in 6 min and 6 h, respectively, and after 8 times reuse, the degradation rates of antibiotics remained at 81.46 % and 76.12 %, which showed excellent reusability and stability. Overall, Lac@UiO-66-NH2(30) is expected to be an ideal candidate for pollution control.

Reduction of heat stress pressure and activation of photosystem II repairing system are crucial for citrus tolerance to multiple abiotic stress combination.

Drought, heat and high irradiance are abiotic stresses that negatively affect plant development and reduce crop productivity. The confluence of these three factors is common in nature, causing extreme situations for plants that compromise their viability. Drought and heat stresses increase the saturation of the photosystem reaction centers, increasing sensitivity to high irradiance. In addition, these stress conditions affect photosystem II (PSII) integrity, alter redox balance of the electron transport chain and decrease the photosynthetic rate. Here, we studied the effect of the stress combinations on the photosynthetic apparatus of two citrus genotypes, Carrizo citrange (Citrus sinensis × Poncirus trifoliata) and Cleopatra mandarin (Citrus reshni). Results obtained showed that physiological responses, such as modulation of stomatal aperture and transpiration rate, aimed to reduce leaf temperature, are key to diminishing heat impact on photosynthetic apparatus and increasing tolerance to double and triple combinations of drought, high irradiance and high temperatures. By using transcriptomic and proteomic analyses, we have demonstrated that under these abiotic stress combinations, Carrizo plants were able to increase expression of genes and proteins related to the photosystem repairing machinery (which better maintained the integrity of PSII) and other components of the photosynthetic apparatus. Our findings reveal crucial physiological and genetic responses in citrus to increase tolerance to the combination of multiple abiotic stresses that could be the basis for breeding programs that ensure a sustainable citrus production.

Aperture Modulation of Isoreticular Metal Organic Frameworks for Targeted Antitumor Drug Delivery.

The introduction of different pore diameters in metal organic frameworks (MOFs) could adjust their drug delivery performance. MOFs with customized structures have potential application value in targeted drug delivery. However, no research on this topic has been found so far. In this report, isoreticular metal organic frameworks (IRMOFs) have been taken as a typical case of tailor-made MOFs, the pore size of which is enlarged (average BJH pore sizes of about 2.43, 3.06, 5.47, and 6.50 nm were determined for IRMOF-1, IRMOF-8, IRMOF-10, and IRMOF-16, respectively), emphasizing the relationship between pore size and model drugs (Oridonin, ORI) and clarifying its potential working mechanism. IRMOF-1, whose pore size matches the size of ORI, has an outstanding drug loading capacity (57.93% by wt) and release profile (about 90% in 24 h at pH 7.4). IRMOF-1 was further coated with polyethylene glycol (PEG) modified with a cell penetrating peptide (CPP44) bound to M160 (CD163L1) protein for targeting of hepatic tumor lines. This nanoplatform (CPP44-PEG@ORI@IRMOF-1) exhibited acid-responsive drug release behavior (37.86% in 10 h at pH 7.4 and 66.66% in 10 h at pH 5.5) and significantly enhanced antitumor effects. The results of cell targeting and in vivo animal imaging indicated that CPP44-PEG@ORI@IRMOF-1 may serve as a tumor-selective drug delivery nanoplatform. Toxicity assessment confirmed that PEGylated IRMOF-1 did not cause organ or systemic toxicity. Furthermore, it is encouraging that the IRMOF-based targeted drug delivery system with pore size modulation showed rapid clearance (most administered NPs are metabolized from urine and feces within 1 week) and avoided accumulation in the body, indicating their promise for biomedical applications. This MOF-based aperture modulation combined with a targeted modification strategy might find broad applications in cancer theranostics. Thus, it is convenient to customize personalized MOFs according to the size of drug molecules in future research.

Preharvest Hydrogen Peroxide Treatment Delays Leaf Senescence of Chinese Flowering Cabbage During Storage by Reducing Water Loss and Activating Antioxidant Defense System.

Leaf yellowing, an indicator of senescence, reduces commercial value of Chinese flowering cabbage after harvest. Hydrogen peroxide (H2O2) plays a dual role in mediating plant stress responses, but it is not clear whether and how it affects leaf senescence when exogenously stimulating the plants before harvest. Here, we found that preharvest application with low concentrations of H2O2 to root delays leaf senescence. Around 10 mM H2O2 reduced leaf yellowing rate by 8.2 and 26.4% relative to the control following 4 and 8 days storage, respectively. The H2O2-treated cabbages showed higher chlorophyll and lower relative expression of senescence-associated gene (SAG) BrSAG12 than the control. Proteomic analysis revealed 118 and 204 differentially expressed proteins (DEPs) in H2O2-treated plants at 4 and 8 days of storage, respectively. The main DEPs are involved in chlorophyll degradation and synthesis, water deprivation, antioxidant activity, and protections on chloroplast membranes. Decline of water loss in H2O2-treated cabbages was coincide with increase of proline contents and modulation of leaf stomatal aperture. Alleviation of oxidative stress was indicated by suppression of respiratory burst oxidase homolog and upregulation of reactive oxygen species (ROS) scavenging-related genes. These results were also supported by the alleviation of lipid peroxidation and the protections on cell integrity and photochemical efficiency in H2O2-treated group. Collectively, preharvest H2O2 treatment alleviates water loss and activates antioxidant defense system, protects chloroplast membrane from oxidative damage, and ultimately delays leaf senescence during storage. This study provides novel insights into the roles of H2O2 for regulating leaf senescence of Chinese flowering cabbage.

A New Player in Jasmonate-Mediated Stomatal Closure: The Arabidopsis thaliana Copper Amine Oxidase β.

Plant defence responses to adverse environmental conditions include different stress signalling, allowing plant acclimation and survival. Among these responses one of the most common, immediate, and effective is the modulation of the stomatal aperture, which integrates different transduction pathways involving hydrogen peroxide (H2O2), calcium (Ca2+), nitric oxide (NO), phytohormones and other signalling components. The Arabidopsis thaliana copper amine oxidases β (AtCuAOβ) encodes an apoplastic CuAO expressed in guard cells and root protoxylem tissues which oxidizes polyamines to aminoaldehydes with the production of H2O2 and ammonia. Here, its role in stomatal closure, signalled by the wound-associated phytohormone methyl-jasmonate (MeJA) was explored by pharmacological and genetic approaches. Obtained data show that AtCuAOβ tissue-specific expression is induced by MeJA, especially in stomata guard cells. Interestingly, two Atcuaoβ T-DNA insertional mutants are unresponsive to this hormone, showing a compromised MeJA-mediated stomatal closure compared to the wild-type (WT) plants. Coherently, Atcuaoβ mutants also show compromised H2O2-production in guard cells upon MeJA treatment. Furthermore, the H2O2 scavenger N,N1-dimethylthiourea (DMTU) and the CuAO-specific inhibitor 2-bromoethylamine (2-BrEtA) both reversed the MeJA-induced stomatal closure and the H2O2 production in WT plants. Our data suggest that AtCuAOβ is involved in the H2O2 production implicated in MeJA-induced stomatal closure.

Retrospective analysis of portal dosimetry pre-treatment quality assurance of intracranial SRS/SRT VMAT treatment plans

AbstractBackground:The complexity associated with the treatment planning and delivery of stereotactic radiosurgery (SRS) or stereotactic radiotherapy (SRT) volumetric modulated arc therapy (VMAT) plans which employs continuous dynamic modulation of dose rate, field aperture and gantry speed necessitates diligent pre-treatment patient-specific quality assurance (QA). Numerous techniques for pre-treatment VMAT treatment plans QA are currently available with the aid of several different devices including the electronic portal imager (EPID). Although several studies have provided recommendations for gamma criteria for VMAT pre-treatment QA, there are no specifics for SRS/SRT VMAT QA. Thus, we conducted a study to evaluate intracranial SRS/SRT VMAT QA to determine clinical action levels for gamma criteria based on the institutional estimated means and standard deviations.Materials and methods:We conducted a retrospective analysis of 118 EPID patient-specific pre-treatment QA dosimetric measurements of 47 brain SRS/SRT VMAT treatment plans using the integrated Varian solution (RapidArcTM planning, EPID and Portal dosimetry system) for planning, delivery and EPID QA analysis. We evaluated the maximum gamma (γmax), average gamma (γave) and percentage gamma passing rate (%GP) for different distance-to-agreement/dose difference (DTA/DD) criteria and low-dose thresholds.Results:The gamma index analysis shows that for patient-specific SRS/SRT VMAT QA with the portal dosimetry, the mean %GP is ≥98% for 2–3 mm/1–3% and Field+0%, +5% and +10% low-dose thresholds. When applying stricter spatial criteria of 1 mm, the mean %GP is >90% for DD of 2–3% and ≥88% for DD of 1%. The mean γmax ranges: 1·32 ± 1·33–2·63 ± 2·35 for 3 mm/1–3%, 1·57 ± 1·36–2·87 ± 2·29 for 2 mm/1–3% and 2·36 ± 1·83–3·58 ± 2·23 for 1 mm/1–3%. Similarly the mean γave ranges: 0·16 ± 0·06–0·19 ± 0·07 for 3 mm/1–3%, 0·21 ± 0·08–0·27 ± 0·10 for 2 mm/1–3% and 0·34 ± 0·14–0·49 ± 0·17 for 1 mm/1–3%. The mean γmax and mean γave increase with increased DTA and increased DD for all low-dose thresholds.Conclusions:The establishment of gamma criteria local action levels for SRS/SRT VMAT pre-treatment QA based on institutional resources is imperative as a useful tool for standardising the evaluation of EPID-based patient-specific SRS/SRT VMAT QA. Our data suggest that for intracranial SRS/SRT VMAT QA measured with the EPID, a stricter gamma criterion of 1 mm/2% or 1 mm/3% with ≥90% %GP could be used while still maintaining an in-control QA process with no extra burden on resources and time constraints.

Surpassing the diffraction limit using an external aperture modulation subsystem and related deep learning method

The resolution of conventional imaging systems is inherently restricted by the diffraction limit. To surpass this diffraction barrier, a scheme using an external aperture modulation subsystem (EAMS) and related deep learning network (DLN) is presented in this paper. The EAMS facilitates the realization of various image acquisition strategies and related DLN architectures. In the specific scenario of 3-aperture modulation strategy, the capabilities of this approach are validated both in numerical simulations and experiments. The results show that both the resolution enhancement ability and the image fidelity can be improved by just adding one label data. This framework proposed here provides a more general way to further explore the ability of DLN-based method to surpass the diffraction limit, and permits a rapid data acquisition that enables new opportunities for the training data collection and further super resolution imaging of label-free moving objects, such as living cells.

A novel series of giant cobalt-calixarene macrocycles: ring-expansion and modulation of pore apertures through recrystallization.

The design and synthesis of metallomacrocycles can be quite challenging because the assemblies of such molecular cycles are difficult to control and the products are usually unpredictable. In this work, a novel series of metallomacrocycles, denoted as {Co30-A}, {Co30-B} and {Co32-A} have been synthesized via self-assembly of p-tert-butylthiacalix[4]arene (H4TC4A) and 3,5-pyrazoledicarboxylic acid (H3pdc) with Co2+ ions under solvothermal conditions. Recrystallization of {Co32-A} under different conditions was found to form {Co32-B} and {Co32-C} that have a similar ring structure to that of {Co32-A} but have different molecular packing modes in the lattices, as well as a 40-membered ring {Co40}. These complexes represent the highest-nuclearity metallocalixarene coordination wheels reported to date. Crystallographic studies indicate that all these metallomacrocycles feature wheel-like structures with apertures varing from 11.4 to 20.3 Å. It is noteworthy that {Co32-A} exhibited good efficiency in removing RhB even at low initial concentration (10 ppm) and also excellent adsorption selectivity towards RhB over Na2Fl (RhB = Rhodamine B, Na2Fl = disodium fluorescein). This work not only makes a breakthrough in the synthesis of metallocalixarene macrocycles with high nuclearity and large apertures, but also provides a simple recrystallization approach to realize the ring-expansion and regulation of molecular packing modes of the metallomacrocycles.

Temperature mitigation strategies in Lepidium latifolium L., a sleeper weed from Ladakh himalayas

The plants growing in high altitude Ladakh Himalayas experience significant temperature variations during their vegetative growth. In this study, the photosynthetic response of Lepidium latifolium L., a sleeper weed, was investigated to short term temperature fluctuations. This plant maintained photosynthesis at higher temperatures by modulating the photochemical efficiency of photosystem II. Various physiological mechanisms including VpdL dependent and independent stomatal opening, the increase in the energy fluxes, closing of the reaction centers, and increase in the chlorophyll content play a crucial role in temperature tolerance. An efficient and dynamic non-photochemical quenching involving both zeaxanthin and PsbS dissipated the excess energy during higher temperatures. The degradation of the photosynthetic apparatus is compensated by increased expression of the subunit proteins. Other genetic elements that help in the repair and stabilization of the apparatus were also induced. Allyl isothiocyanate dependent modulation of the stomatal aperture acts as a swift response mechanism for the CO2 exchange and evapotranspirational requirements. Collectively, these processes help mitigate the harmful effects of high temperature on the photosynthetic process. This data will help to strategize the spread of Lepidium latifolium in an ecologically sensitive Ladakh Himalayas during the climate change scenario.

Loss of haptic feedback impairs control of hand posture: a study in chronically deafferented individuals when grasping and lifting objects

Previous work has highlighted the role of haptic feedback for manual dexterity, in particular for the control of precision grip forces between the index finger and thumb. It is unclear how fine motor skills involving more than just two digits might be affected, especially given that loss of sensation from the hand affects many neurological patients, and impacts on everyday actions. To assess the functional consequences of haptic deficits on multi-digit grasp of objects, we studied the ability of three rare individuals with permanent large-fibre sensory loss involving the entire upper limb. All three reported difficulties in everyday manual actions (ABILHAND questionnaire). Their performance in a reach–grasp–lift task was compared to that of healthy controls. Twenty objects of varying shape, mass, opacity and compliance were used. In the reach-to-grasp phase, we found slower movement, larger grip aperture and less dynamic modulation of grip aperture in deafferented participants compared to controls. Hand posture during the lift phase also differed; deafferented participants often adopted hand postures that may have facilitated visual guidance, and/or reduced control complexity. For example, they would extend fingers that were not in contact with the object, or fold these fingers into the palm of the hand. Variability in hand postures was increased in deafferented participants, particularly for smaller objects. Our findings provide new insights into how the complex control required for whole hand actions is compromised by loss of haptic feedback, whose contribution is, thus, highlighted.

High-resolution separation of DNA/proteins through nanorod sieving matrix

In this paper, we report on an integrated nanorod array as a novel sieving matrix for high-resolution separation of biomolecules. A wide size range of DNA (100 bp–166 kbp) and proteins (11.4 kDa–205 kDa) are resolved into sharp peaks within several minutes using devices with various pore apertures, either in a reptation mode or Ogston mechanism. The device delivers notable performance with the minimum resolvable size difference 10 bp for 100 bp and 150 bp DNA fragments, 15.9 kbp for 48 kbp and 166 kbp DNA chains, and 7.9 kDa for 11.4 and 68 kDa proteins, where the separation efficiency is higher than or comparable to other micro/nanofabricated artificial sieving structures. The process utilizes localized oblique angle deposition (LOAD) to simply integrate densely packed nanorods onto steep sidewalls of microfluidic channels, which eliminates the requirement of advanced lithography tools routinely used in conventional artificial sieving structures. We further demonstrate the modulation of pore aperture that employs the sculptured sidewalls by a custom deep reactive-ion etching (DRIE) recipe. Given the easy fabrication, excellent engineering control and outstanding sieving capability, this nanorod sieve device has great potentials to provide a simple and effective separation technique for biomolecule analysis.

Gamma-ray modulation properties of tungsten coded apertures for a novel mixed-field imaging system

An investigation into the gamma-ray modulation properties of a tungsten coded aperture, whose design is based on the mathematical principles of Modified Uniformly Redundant Arrays (MURA), has been performed. Due to the small size of the individual cells, the aperture was built using additive manufacturing methods. The gamma-ray field was produced by a 137Cs radioactive isotope at Lancaster University, U.K. An organic plastic scintillator sample, which is capable of pulse shape discrimination, has been used to detect the gamma-ray field modulated by a tungsten aperture. Prior to the investigation of the aperture modulation properties, energy calibration of the scintillator was performed. Its pulse shape discrimination capabilities were verified using a 252Cf fission source. In this study, each of 169 coded aperture cells was investigated by collimating the modulated gamma-ray field of 137Cs through a 25.4 mm thick lead supporting plate. The supporting plate has one opening in the centre, of the same dimensions as the single aperture cell, i.e. 2.5 mm × 2.5 mm. The number of pulses detected for every aperture location were recorded in an array. The array was subsequently used to create a two-dimensional image of the source, which was encoded through the coded aperture pattern. Finally, the image was decoded using deconvolution techniques to reveal the actual source location. The new results obtained in this study indicate that sufficient gamma-ray modulation properties of the aperture can be determined, despite the relatively small footprint and thickness of the coded aperture.

Fine-Tuning Stomatal Movement Through Small Signaling Peptides

As sessile organisms, plants are continuously exposed to a wide range of environmental stress. In addition to their crucial roles in plant growth and development, small signaling peptides are also implicated in sensing environmental stimuli. Notably, recent studies in plants have revealed that small signaling peptides are actively involved in controlling stomatal aperture to defend against biotic and abiotic stress. This review illustrates our growing knowledge of small signaling peptides in the modulation of stomatal aperture and highlights future challenges to decipher peptide signaling pathways in guard cells.

Organ at risk dose verification in nasopharyngeal cancer VMAT planning with collimator comparison between single arc and double arc

Volumetric modulated arc therapy (VMAT) is an irradiation technique that enables the improvement of the flexibility of dose delivery from a full range of angles with continuous modulation of beam aperture and dose rate. We compared the homogeneity index (HI), conformity index (CI), dose volume histogram (DVH) and dose differentiation of single arc and double arc VMAT delivered for nasopharyngeal cancer (NPC). Planning for gantry rotation angles of 360° counter clockwise and clockwise (double arc) with collimator (0° and 10°) and 360° clockwise (single arc) were created for ten nasopharyngeal cancer cases applied in RANDO phantom with the Eclipse treatment planning system and delivered using Trilogy linear accelerator. The verification was performed by using gafchromic film EBT3 and placed on the RANDO phantom according to the location of the organs at risk. In this study, we evaluated right parotid, left parotid, right eye, left eye and trachea. The measurement of mean dose using gafchromic film and TPS shows that the resulting values were close to TPS results with the difference being −1.51%. From the result of dose measurement using gafchromic film based on the data from RANDO CT phantom use, it can be concluded that double arc VMAT technique with collimator can reduce the dose in the organ at risk.

Super-resolution imaging via aperture modulation and intensity extrapolation

High-resolution telescopic imaging is of great importance in astronomy. Compared to the complexity and huge cost of constructing extremely-large telescopes, super-resolution technique which breaks the diffraction limit of the imaging system can enhance the spatial resolution with compact setup and low cost. In this paper, a novel super-resolution telescopic imaging method based on aperture modulation and intensity extrapolation is demonstrated, with both simulated and experimental studies performed. The simulation results show that the method can enhance the resolving power of a diffraction-limited telescopic imaging system by >5 times in noise-free case, and the improvement still reaches ~1.8 times with a signal-to-noise ratio of only ~10. The preliminary experimental results show a resolution enhancement of ~1.36 times for the limitations of the experimental setup. Better performance is possible with the images for reconstruction denoised and registered more precisely. The method is also useful in wide-field microscopy.

Roles of pepper bZIP protein CaDILZ1 and its interacting partner RING-type E3 ligase CaDSR1 in modulation of drought tolerance.

Abscisic acid (ABA) is a plant hormone that plays a key role in the environmental stress response, especially the induction of ABA-responsive and stress-responsive genes and modulation of the stomatal aperture in response to drought stress. Here, we identified CaDILZ1 (Capsicum annuum Drought-Induced Leucine Zipper 1) belonging to subgroup D of the bZIP protein family; gene functions of this family in response to ABA and drought signaling still remain unknown. CaDILZ1 expression was significantly induced in pepper leaves after exposure to ABA, drought, and NaCl. The CaDILZ1 protein localized in the nucleus of plant cells. In response to drought stress, CaDILZ1-silenced pepper and CaDILZ1-overexpressing Arabidopsis plants exhibited drought-sensitive and drought-tolerant phenotypes, respectively, via altered ABA content, stomatal closure, and expression of ABA-responsive and drought-responsive marker genes. We isolated the RING finger protein CaDSR1 (Capsicum annuum Drought Sensitive RING finger protein 1), which interacted with CaDILZ1 in the nucleus. The CaDSR1 protein exhibited E3 ligase activity and promoted CaDILZ1 degradation via the 26S proteasome pathway. Under drought stress conditions, CaDSR1-silenced pepper and CaDSR1-overexpressing Arabidopsis plants exhibited contrasting phenotypes to those of CaDILZ1-silenced pepper and CaDILZ1-overexpressing Arabidopsis plants. Taken together, our data suggest that CaDSR1 and CaDILZ1 function in ABA-mediated drought stress signaling in pepper plants.

Photon optimizer (PO) vs progressive resolution optimizer (PRO): a conformality- and complexity-based comparison for intensity-modulated arc therapy plans

This study aimed to provide guidance on the advantages and limitations of a new optimizer, “photon optimizer” (PO), when compared with its predecessor, “progressive resolution optimizer” (PRO), for intensity-modulated arc therapy (IMAT) plans.Eleven study plans that included a cohort of prostate, head and neck, and brain treatment sites were optimized using both PRO and PO algorithms. A plan template using the same objectives for the same number of iterations was used for each optimized plan to obtain hypothetical treatment plans that would be comparable with a clinical plan. Analysis was performed using plan conformity-based parameters such as target volume coverage factor, conformation number and homogeneity indices, and plan complexity assessment parameters such as small aperture score, modulation indices, and monitor unit variation with arc angle for prostate, brain and head, and neck IMAT treatment plans.Plan conformality analysis demonstrated that conformation numbers, target volume coverage factors, and homogeneity indices produced by the 2 optimizers were comparable for most anatomic sites. IMAT treatment plans produced using the PRO optimizer were found to be less complex than plans produced using the PO optimizer, in terms of multileaf collimator (MLC) leaf position variability and modulation complexity scores. Similarly, the PRO optimizer was shown to produce treatment plans that used fewer monitor units (and generally fewer monitor unit per degree of arc rotation) than PO optimizer.This study demonstrated that the PO optimizer can produce IMAT treatment plans with a similar degree of dose conformity to the target volume and generally improved organ at risk sparing, compared with the PRO optimizer. Better coverage to organs at risk produced by plans optimized using PO was observed to have higher MLC variability and monitor units. Therefore, careful evaluation of treatment plan conformity and complexity before assessing its deliverability is recommended when implementing the routine use of PO optimizer.