Greenhouse whitefly, Trialeurodes vaporariorum, is a polyphagous pest that causes economic loss. Management is based on constant application of synthetic insecticides. Development of cultivars resistant to insect vectors is an alternative that can reduce infestation and infection levels. In addition, it is a strategy that may be implemented effectively as part of an integrated management system. The objective of this report was to evaluate the effect of antibiosis and antixenosis of three tomato varieties on T. vaporariorum. Under laboratory and greenhouse conditions, settlement, oviposition, and nymph development were evaluated. Wild variety Solanum habrochaites was least attractive to adults in antixenosis tests, demonstrating low growth in insect nymphs in antibiosis tests compared to S. lycopersicum cv. Floradade. Variety S. pimpinellifolium showed moderate resistance in both evaluations. The high level of resistance of LA0407 can serve as a base for tomato breeding, focusing on development of resistant cultivars.