Antioxydant Activity Research Articles

DETERMINATION OF POLYPHENOLIC COMPOUNDS IN PHYTOEXTRACTS WITH ANTIBACTERIAL PROPERTIES AND STUDY POTENTIAL EFFECTS

Objectives. The research work is a comparative study of the polyphenolic compounds and flavonoid content of the phytoextracts obtained from the ultrasonic extraction process (Stegler DG-360) with 95% ethanol and ethanol-propyleneglycol mixture (in 95 : 5 ratio) from the Clove buds (Caryophyllos aromaticus L. Myrtaceae), hibiscus flowers (Hibiscus sabdariffa L. (Hs, roselle; Malvaceae)) and common yarrow (Achillea millefolium L. Asteraceae) plants, which are rich in biologically active substances with high therapeutic effect, as well as their devoted to the investigation of antioxydant activity and antimicrobial potential. Material and methods. The research work was carried out in the Central Research and Application Laboratory of Ağrı İbrahim Çeçen University (MERLAB), Republic of Turkey, using a Japanese Shimadzu brand Mistral-AB SCIEX 4000 QTRAP liquid chromatograph-mass spectrometer device. Results.It was found that in both extraction processes, the active substances penetrated sufficiently from the medicinal-plant raw materials into the extractant. 20 names of compounds containing polyphenol, flavonoid and anthocyanin derivatives have been determined. However, the amount of active substances in the phytoextract obtained with the presence of the penetrate (propylene glycol) was higher. In particular, rutin ( 75.74 ± 4.91mg/g), quercetin (110.12 ± 1.30 mg/g), gallic acid (4503.19 ± 46.83mg/g), quinic acid (4041.99 ± 187.3 mg/g), chlorogenic acid (582.30 ± 1.30mg/g), vanilin (155.6g/g1 ± 3.95mg/g) prevailed. Conclusion. The research work proves once again that the studied phytoextracts are rich in polyphenolic compounds and flavonoids. The amount of biologically active substances in the phytoextract obtained with the ethanol-propylene glycol mixture which is the penetrate substance (in the ratio of 95:5) is higher than that of the other sample (with 95% ethanol). The resulting amount of biologically active substances is a clear proof that the phytoextract has sufficient antioxidant and antimicrobial properties.

Assessment of Antihypoxic Activity of S-analoges of Dihydropyrimidin-2-ones and Their Condensed Derivatives

Introduction. Antioxydant activity, membrane stabilizing and cytoprotective effect of dihydropyrimidin analoges are widely described in various literary sources. One of the importantant mechanisms of action of antioxidants is their antihypoxic activity. It is the ability to increase tissue resistance to hypoxia. The search for drugs to increase the body's resistance under hypoxic conditions is an actual task in experimental and clinical pharmacology.Aim. Study of the antihypoxic activity of new S-analogs of dihydropyrimidin-2-ones and their condensed derivatives in various models of hypoxia.Materials and methods. 13 compounds S-analogs of dihydropyrimidin-2-ones and their condensed derivatives are objects of research. Their structure has been confirmed by spectroscopy methods. The antihypoxic activity was carried out by recommended methods for preclinical study of new pharmacological substances.Results and discussion. This paper presents the results of testing the obtained compounds of different structures on three models of hypoxia. The activity of a number of condensed tricyclic derivatives of dihydropyrimidine-2-thiones was studied in more detail using a model of acute normobaric hypoxia with hypercapnia.Conclusion. The compounds did not demonstrate high antihypoxic activity; some «structure-property» patterns were revealed.

Impact of Extraction Solvent Polarity: Antioxydant Activity of Methanolic, Hydromethanolic and Aqueous Decocted Extracts of Algerien Thymelaea hirsuta (L.) Endl. Areal Parts

Thymelaea hirsuta (L.) Endl. , known as ‘Methnane’ in Algeria, is a widely medicinal plant used in folk medicine. In the present study, In vitro antioxidant activity of T. hirsta extracts and the impact of extraction solvent polarity on the antioxidant potential were investigated. Three types of polar solvents with decreasing polarity were chosen; water for decocted extract, methanol-water at 50% and absolute methanol for macerated extracts. Total phenolic and flavonoid contents were evaluated and showed a high amount wihch decreases with increasing polarity. Antioxydant activity was assessed with different methods: ABTS assay for evaluation of scavenge activity, CUPRAC and reducing power for assessement of the reduction potential of T. hirsuta areal parts. The results showed that T. hirsuta areal parts exhibited a strong scavenging activity with significant difference between extracts in terms of their polarity. In the same line, the most polar aqueous decocted extract exhibited a considerable reducing activity followed by hydromethanolic and methanolic extract with increasing potential. These findings suggest the suitability of polar solvents for the extraction of phytochemical compounds from T. hirsuta areal parts and so, their antioxidant activity against several radicals and ions.

In vitro and in silico antioxydant activity, toxicity prediction, and molecular docking study of 3- and 3,3'-nitrophenylferrocene and their reduced amines

The antioxidant activity of 3-nitrophenylferrocene (3NPF) and 3,3'-nitrophenylferrocene (3,3'NPF) and their reduced amines was measured using superoxide anion radical (). Binding parameters such as binding free energies and binding constants were also calculated. sign and values suggest respectively the spontaneity and a strong interaction between the radical and all studied compounds. Molecular docking study showed that 3NPF is most inreactive compound against glutathione reductase enzyme having the the lowest docking scores of -16.96 kJ/mol. The two reduced forms were predected to be non-toxic and are not inhibitors of CYP450 2C19, 2D6 isoenzymes which suggests a decrease in their plasma concentrations and a rapid elimination route.

Comparative Study of the Antioxidant Power of Polyphenols of Leaves, Fruits, and Bark of Pistacia atlantica Desf. from Morocco

Pistacia atlantica Desf. is a widely used plant species in the traditional medicine for its various pharmacological properties. The aim of this study is the valorisation of polyphenols and the antioxidant power of three organs from Pistacia atlantica Desf. (leaves, fruits, and bark). Phytochemical screening of secondary metabolites was performed using precipitation and coloring reactions. Total phenols, flavonoids, and condensed tannins were quantified, respectively, by the Folin-Ciocalteu method, the aluminum trichloride method, and the vanillin method. Evaluation of the antioxidant activity was made using two methods: DPPH∗(2,2′-diphenyl-1-picryl hydrazyl) and FRAP (ferric reducing antioxidant power). The obtained results showed that the three organs of Pistacia atlantica are rich in total phenols, flavonoids, and condensed tannins. The highest contents of total phenols and flavonoids are recorded by the crude fruit extract, 723 mg EGA/gE and 34.57 mg EQ/gE, respectively, while the highest content of condensed tannins is recorded in the butanolic extract of the leaves (997.58 mg ECat/gE). The antioxidant activity of the three organs extracts from Pistacia atlantica Desf. confirmed their strong antiradical power, much higher than that of ascorbic acid and BHA (butylated hydroxyanisole). Indeed, the concentration of the crude extract of P. atlantica leaves reducing 50% of the DPPH∗free radicals (IC50) is 27.22 μg/ml. This concentration is much lower than those of ascorbic acid (31.44 μg/ml) and BHA (46.25 μg/ml). The antioxidant power using the FRAP method has also shown that the leaves extract of this species has a much higher reducing capacity of iron than those of the reference standards. A positive correlation between antioxidant capacities and flavonoids contents of leaves and fruits extracts was observed. The two methods (DPPH∗and FRAP) proved that the three organ extracts of Pistacia atlantica possess very remarkable antioxydant activity. These extracts could be exploited as natural antioxidants against the oxidation phenomenons and the oxidative stress in several fields (food, cosmetic, pharmaceutic, and others).

Antioxidant and cytotoxicity activities of δ-tocotrienol from the seeds of Allophylus africanus

Chemical investigation of Allophylus africanus P. Beauv fruits led to the isolation of a new δ-tocotrienol, 3α-hydroxy-δ-tocotrienol (1) together with eight known compounds (2-9). Compound (1) was allylated (1a) and prenylated (1 b and 1c) to give three new semi-synthesized derivatives which were fully characterized as: 6-O-allyl-3α-hydroxy-δ-tocotrienol (1a), 6-O-prenyl-3α-hydroxy-δ-tocotrienol (1 b) and 6-O,5-C-diprenyl-3α-hydroxy-δ-tocotrienol (1c). The structures of compounds were established using comprehensive spectroscopic analysis including UV, MS, 1 D NMR, 2 D NMR and by comparison with the corresponding literature data. Compound (1) and its semi-synthetic derivatives (1a-c) were tested for their antioxydant activity using DPPH radical scavenging assay and also for their cytotoxicity using human cervix carcinoma KB-3-1 cell lines. The results showed that compound (1) exhibited antioxidant activity with an IC50 value of 0.25 μM compared to the reference control trolox (26 µM); and good cytotoxic activity with IC50 values of 97 μM compared to the reference (+)-griseofulvin (IC50 between17-21 μM).

Phytochemical screening and evaluation of antioxidant activity of the stem bark of Anacardium occidentale L. and Vitellaria paradoxa C.F. Gaertn, two medicinal plants from Poro region (Northern of Côte d'Ivoire)

Anacardium occidentale and Vitellaria paradoxa are two medicinal plants traditionally used by Ivorian, particularly in the treatment of diabetes, arterial hypertension and diarrhea. The objective of this work was to evaluate the phytochemical constituents and the antioxidant activity of the stem barks of A. occidentale L. and V. paradoxa. The stem bark of both plants was used for the aqueous extraction. Major chemical groups were identified using the tube staining method followed by confirmation by thin layer chromatography (TLC). The antioxidant activity of the extracts was evaluated by DPPH method. The results of phytochemical screening of plant extracts revealed the presence of polyphenols, anthraquinones, tannins, sterols, terpenes and saponins. In addition, the evaluation of the antioxidant activity by spectrophotometry using the DPPH method showed that all extracts had significant and different antioxydant activity compared to the reference molecule (Vitamin C, IC50 = 0.80 ± 0.70 µg/mL) with respective IC50 of 0.42 ± 0.32 µg/mL for V. paradoxa and 0.87 ± 0.77 µg/mL for A. occidentale. V. paradoxa therefore has the best antioxydant activity. These results could explain the therapeutic virtues of A. occidentale and V. paradoxa in the treatment of various pathologies.

Ktivitas Antioksidan Dan Sifat Sensoris Teh Herbal Celup Kulit Anggur (Vitis vinifera L.) Pada Suhu Dan Waktu Pengeringan


 
 
 The research aimed to determine the effect of drying temperature and time on antioxidant activity and sensory properties of herbal tea bag of grape skin and to obtain the best of temperature and drying time that can produce grape skin herbal tea bag with highest antioxidant activity and the best sensory properties.This research used a completely randomized design with factorial pattern in two factors namely, drying temperature (50oC, 60oC, 70oC) and drying time (3.0 hours, 3.5 hours, 4.0 hours). There are 9 treatment combinatioms, each treatment combination was repeated 2 times so that it obtained 18 experimental units. Parameters observed were water content, total phenols, total falvonoids, antiokxidant activity, total anthocyanin, and sensory properties. The data obtained were analyzed by analysis of variance. If the treatment had a significant effect, it would be followed by the Duncan Multiple Range Test. The results showed that interaction beetwen drying temperature and time treatment had a significant (P<0.01) on antioxidant activity, total phenols, total falvonoids, total anthocyanin, extract content in water, water content, and color (scoring test), but no significant (P>0.05) on the color (hedonic test), aroma (hedonic test), taste (hedonic test), and overall acceptance. The results showed a drying temperature of 60oC with a drying time of 3.0 hour was the optimum drying temperature and time to produce herbal tea bag of grape skin with antioxydant activity of (IC50 1512.17 ppm), water content of 5.60%, total phenol content 9.21 mg GAE/g, total flavonoids content of 6.15 mg QE/g, total anthocyanin content 8.39 mg/g, extract content 41.85%, dark purple color with hedonic color, taste, aroma and overall acceptance is neither like nor dislike.
 
 

Aloe vera L. (Asphodelaceae): Supplementation of in-vitro culture medium with Aloe vera gel for production of genetically stable plants

As in vitro culture environments can be mutagenic, it's challenging to produce true-to-true type plants from meristems. Therefore, the present study aimed to devlop an efficient protocol for micropropagation from shoot tip explants of medicinally important Aloe vera using different concentrations of A. vera leaf gel (AvG) as organic supplement to the culture media. Assessment of the genetic stability of A. vera in vitro multiplied plants compared to the donor plant is one of the most important pre-requisites especially before its use for commercial and medicinal purposes. For shoot multiplication, the highest number of axillary shoots recorded was 13.27 ±5.11 with an average length of 2.21 ±0.84 cm on M3 medium (MS + 0.2 mg L−1 of β-indole butyric acid (IBA) + 3 mg L−1 benzylamino-purine (BA) + 5% AvG). All three strength of MS medium (1, 1/2, 1/4) with different concentrations of AvG resulted in 100% root induction after 4 weeks of culture. All the rooted microshoots (100%) were successfully acclimatized. To provide a reliable conclusion on its nutritive supply to MS medium, the total phenolic (TPC), condensed tannins (TTC), flavonoid (TFC), and flavonol (TFlC) content as well as the antioxydant activity of AvG were investigated. TPC and TTC values were found to be 31.02 ± 0.46 μg gallic acid equivalent mg−1 of extract and 4.00 ± 0.96 μg catechin equivalent mg−1 of extract, respectively while TFC and TFlC were 13.58 ± 0.84 and 9.75 ± 0.58 μg rutin equivalent mg−1 of extract, respectively. The results of antioxydant activity of AvG following DPPH, FRAP and ABTS assays were equal to 12.50 ± 0.89; 49.59 ± 2.07 and 64.93 ± 3.23 µmol Trolox equivalent mg−1 of extract, respectively which may explain the positive role of AvG as supplement.The genetic stability of in vitro propagated A. vera plants was evaluated using 10 inter simple sequence repeat (ISSR), 10 start codon targeted (SCoT) and 10 simple sequence repeat (SSR) markers. Out of these, 5 ISSR, 3 SCoT and 6 SSR primers produced resolvable, reproducible and scorable bands. ISSR primers generated 32 amplification products ranging from 200 to 1040 bp in size with a mean number of 6.4 bands while SCoT primers generated 33 amplicons with an average of 11 bands in the range of 160–2410 bp. In the SSR products, 6 bands were observed, each primer produced one band ranging from 410 to 720 bp. All banding profiles were monomorphic proving that the stability of the micropropagated A. vera plants was maintained. This study proved the suitability of supplementing in vitro culture media with AvG to enhance direct regeneration through shoot tip explants of A. vera for large scale micropropagation applications.

Antioxydant activity of the silver nanoparticles (AgNPs) synthesized using Nephrolepisradicans extract as bioreductor

The silver nanoparticle is a silver metal in nanosize which is currently widely applied in various fields, such as optics, electronics, biology, catalysts, health, food, and the environment. Silver nanoparticles can be synthesized by top-down and bottom-up methods. Bottom-up method can be done by reacting silver nitrate solution with plant extracts or known as green synthesis. This research is aimed to synthesis the silver nanoparticles using the methanolic extracts of Nephrolepisradicansferns as bioreductor and testing their antioxidant activity. The characterization of silver nanoparticles synthesized was carried out using the UV and FTIR spectrophotometer. The antioxidant activity test was carried out using the DPPH method. The results showed that the silver nanoparticles synthesized had an average diameter of 19,194 nm. Based on the IR spectrum, the functional groups that played an important role in the process of synthesis of silver nanoparticles were hydroxyl (OH), carbonyl (C = O) and ether (C-O). The silver nanoparticles had antioxidant activity in the medium category with IC50 of 106.442 ppm. Thus, the silver nanoparticles synthesized had the potential to be developed as antioxidants agent.

CHEMICAL ANALYSIS, ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTINOCICEPTIVE EFFECTS OF ACETONE EXTRACT OF ALGERIAN SOLENOSTEMMA ARGEL (DELILE) HAYNE LEAVES

Objective: To investigate the qualitative composition of the acetonic extract from leaves of S. argel (AESA) and their anti-inflammatory and analgesic properties in vivo.
 Methods: AESA profile was established by UHPLC/DAD/ESI-MS2. AESA was subjected to the acute oral toxicity study according to the OECD-420 method. Antioxydant activity of AESA was performed by DPPH radical scavenging assay. Anti-inflammatory effects of AESA were determined in two animal models: carrageenan-induced paw edema in rats and cotton pellet-induced granuloma formation in rats. Further, anti-nociceptives activities of AESA were assessed by hot plate test, acetic acid-induced abdominal writhing test and formalin test.
 Results: The in vivo AESA toxicity was low. AESA expresses a maximum radical scavenging activity with a IC50 value of 36,05 μg/ml. The AESA at 250 and 400 mg/kg significantly reduced carrageen an induced paw edema by 70.09% and 85.53% 6h after carrageenan injection, respectively. AESA produced significant dose-dependent anti-inflammatory effect against cotton pellets-induced granuloma formation in rats. In addition, AESA at 250 and 400 mg/kg significantly reduced acetic acid-induced writhing by 56.83 and 80.41%, respectively. Oral administration of 250 and 400 mg/kg of AESA caused a significant dose dependent anti-nociceptive effect in both neurogenic and inflammatory phases of formalin-induced licking. AESA also impacted the pain latency in the hot plat test.
 Conclusion: These data suggest that AESA possesses antioxidant, anti-inflammatory and anti-nociceptive effects. These results support the traditional use of S. argel to cure pain and inflammatory diseases in the Algerian Sahara.

Total of phenol and antioxydant activities of rice bran yoghurt

<p align="center"><strong>ABSTRAK</strong></p><p><strong><em>Latar Belakang: </em></strong><em>Bekatul merupakan salah satu produk pertanian Indonesia yang kaya akan zat bioaktif, terutama senyawa fenolik dan fitokimia. Senyawa fenolik sulit diekstraksi karena seratnya tidak larut air. Salah satu cara untuk mengekstraksinya yaitu dengan fermentasi. Yogurt merupakan salah satu produk fermentasi susu. Adanya penambahan bekatul ke dalam yogurt dapat meningkatkan efek fungsionalnya, tidak hanya efek kesehatan saluran cerna, tetapi juga efek antioksidatifnya.</em></p><p><strong><em>Tujuan: </em></strong><em>Untuk mengetahui pengaruh proses fermentasi terhadap aktivitas antioksidan dan total fenol yogurt dengan adanya penambahan bekatul.</em></p><p><strong><em>Metode: </em></strong><em>Penelitian ini merupakan penelitian eksperimental menggunakan rancangan acak lengkap. Ada 4 formula yogurt yang berbeda pada jumlah bekatul yang ditambahkan, antara lain: 0%, 5%, 10%, dan 15%. Total fenol dianalisis menggunakan metode Follin-Ciocalteu dan aktivitas antioksidan dianalisis dengan metode DPPH. Data kemudian dianalisis statistika menggunakan one-way ANOVA dan dilanjutkan dengan uji beda nyata Duncan.</em></p><p><strong><em>Hasil: </em></strong><em>Penambahan bekatul pada konsentrasi yang lebih tinggi memengaruhi persentase total fenol yang makin tinggi juga, antara lain 0,03±0,00; 0,04±0,00; 0,06±0,00; dan 0,07±0,00%</em> <em>untuk penambahan konsentasi bekatul 0, 5, 10, dan 15%. Aktivitas antioksidan juga meningkat secara signifikan dari 25,77±0,14% untuk sampel tanpa penambahan bekatul menjadi 47,43±0,29% untuk sampel dengan penambahan bekatul 15%.</em></p><p><strong><em>Kesimpulan: </em></strong><em> Penambahan bekatul memiliki efek yang signifikan terhadap peningkatan total fenol dan aktivitas antioksidan yogurt.</em></p><p><em> </em></p><p><strong>KATA KUNCI</strong><strong><em>: </em></strong><em>bekatul, </em><em>yogurt, </em><em>aktivitas </em><em>antio</em><em>ksidan</em><em>, total phenol</em></p><p><em> </em></p><p align="center"><strong> </strong></p><p align="center"><strong>ABSTRACT</strong></p><p><strong><em>Background</em></strong><em>: </em><em>Rice bran is one of Indonesia agricultural product that is high</em><em> </em><em>in</em><em> bioactives</em><em>, especially </em><em>phenolic and phytochemical compounds</em><em>.</em><em> Phenolic compunds are difficult to extract because the fiber can not solute in water. An alternat</em><em>ive way to extract</em><em> </em><em>is </em><em>b</em><em>y</em><em> </em><em>fermentation.</em><em> </em><em>Yogurt is one of dairy fermentation product. The addition of rice bran into the yogurt will improve its functional effect, not only provides the gastrointestinal health effects but also antioxidant activity. </em></p><p><strong><em>Objective</em></strong><strong><em>s</em></strong><strong><em>:</em></strong><em> To </em><em>investigate</em><em> the influence of fermentation process on </em><em>the </em><em>antioxidant activity and total phenol </em><em>of</em><em> yogurt with </em><em>the </em><em>addition of rice bran.</em></p><p><strong><em>Method</em></strong><strong><em>s</em></strong><strong><em>: </em></strong><em>This</em><em> was </em><em>an </em><em>experimental </em><em>study </em><em>using </em><em>completely randomized design. There were 4 formulation of yogurts with different amount of rice bran, i.e.</em><em> 0%, 5%, 10%, </em><em>and </em><em>15%. </em><em>Total phenol was measured by Follin-Ciocalteu methods and the antioxidant activity was analyzed by DPPH. Data were analyzed statistically by one-way ANOVA and continued to Duncan’s multiple range test.</em><em></em></p><p><strong><em>Results: </em></strong><em>The </em><em>addition of </em><em>rice </em><em>bran </em><em>in higher concentration showed higher percentage of </em><em>total phenol</em><em>, those were 0.03±0.00; 0.04±0.00; 0.06±0.00; and 0.07±0.00</em> <em>respectively for the addition of 0, 5, 10, and 15% of rice bran. The antioxidant activities were also increased significantly from 25.77±0.14% for sample without rice bran to 47.43±0.29% for sample with the addition of 15% rice bran. </em><em></em></p><p><strong><em>Conclusion</em></strong><strong><em>s</em></strong><strong><em>: </em></strong><em>The addition of </em><em>rice </em><em>bran </em><em>gave the significant effect on total phenol and</em><em> antioxidant activity </em><em>of yogurt</em><em>.</em></p><p><em> </em></p><p><strong><em>KEYWORDS: </em></strong><em>rice bran</em><em>, </em><em>yogurt, antioxidant activity, total phenol</em></p>

Characterization, antioxidant, antimycobacterial, antimicrobial effcts of Moroccan rosemary essential oil, and its synergistic antimicrobial potential with carvacrol.

Thanks to their promising properties, essential oils (EOs) have strong potential to remedy several problems such as microorganisms acquired resistance to antimicrobial agents and chemical antioxidants toxicity. Firstly, this work was conducted to determine chemical composition, antioxydant activity, then antibacterial, antifungal, antimycobacterial properties of Rosmarinus officinalis EO. Secondly, EOs combined antimicrobial effect with carvacrol was assessed. Chemical EO analysis was performed using Gas chromatography/mass spectrometer. 1,1-diphenyl-2-picrylhydrazyl test was used to evaluate in vitro antioxidant rosemary oil effect. The antimicrobial activity against seven bacteria, two fungi, and two mycobacterial strains was screened using the broth microdilution method. Thereafter, the checkerboard essay was used to evaluate the antibacterial effect of this EO and Carvacrol. Chemical EO analysis revealed 1,8-cineole (33.88%), camphor (14.66%), and α-pinene (12.76%) as main constituents. The obtained IC50 value (2.77 mg/mL) showed rosemary EO's radical scavenging power. Moreover, all tested microorganisms showed an important sensitivity to this EO (MIC values: 0.007%–1% (v/v)). Furthermore, results highlighted synergistic and partial synergistic interaction for tested products. The studied EO has both antimicrobial and antioxidant potentials. Combined application showed a remarkable synergistic antibacterial potentiation that can be used as an alternative in pharmaceutical and food processing sectors.

Identifikasi Senyawa Metabolit Sekunder, Uji Antimikroba dan Antioksidan Ekstrak Akar Gantung Hornstedtia Scyphifera Var. Fusiformis Holttum (Sijangkang)

Hornstedtia scyphifera var. fusiformis Holttum is endemic plant of Zingiberaceae family that distributed in Sumatera. Hornstedtia scyphifera var. fusiformis Holttum contained some secondary metabolite such as phenolic, saponin, triterpenoid and alkaloid. The biological activity of methanol, ethyl acetate, and n-hexane extracts from Hornstedtia scyphifera var. fusiformis Holttum suspended roots was tested for antibacterial and antioxydant activity. Antioxidant activity was analized by DPPH method. The antibacterial activity was tested by used disk difussion method againts Staphylococcus aureus bacteria (gram-positive bacteria) and Escherichia coli (gram-negative bacteria). Almost all of Hornstedtia scyphifera var. fusiformis Holttum suspended roots extract were given antibacterial activity, nonetheless the biggest inhibition zone of Escherichia coli that was inhibited by n-hexane and ethyl acetate extracts which have inhibition zone 10 mm at concentration 500 mg/L and also againts Staphylococcus aureus, the biggest inhibition by ethyl acetate and n-hexane extracts which have inhibition zone 10.30 mm at concentration 500 mg/L. Among all extracts tested, methanol extract of the possessed moderate free radical scavenging activity with IC50 = 51.89 mg/L.

Identifikasi Senyawa Metabolit Sekunder, Uji Antimikroba dan Antioksidan Ekstrak Akar Gantung Hornstedtia Scyphifera Var. Fusiformis Holttum (Sijangkang)

Hornstedtia scyphifera var. fusiformis Holttum is endemic plant of Zingiberaceae family that distributed in Sumatera. Hornstedtia scyphifera var. fusiformis Holttum contained some secondary metabolite such as phenolic, saponin, triterpenoid and alkaloid. The biological activity of methanol, ethyl acetate, and n-hexane extracts from Hornstedtia scyphifera var. fusiformis Holttum suspended roots was tested for antibacterial and antioxydant activity. Antioxidant activity was analized by DPPH method. The antibacterial activity was tested by used disk difussion method againts Staphylococcus aureus bacteria (gram-positive bacteria) and Escherichia coli (gram-negative bacteria). Almost all of Hornstedtia scyphifera var. fusiformis Holttum suspended roots extract were given antibacterial activity, nonetheless the biggest inhibition zone of Escherichia coli that was inhibited by n-hexane and ethyl acetate extracts which have inhibtion zone 10 mm at concentration 500 mg/L and also againts Staphylococcus aureus, the biggest inhibition by ethyl acetate and n-hexane extracts which have inhibition zone 10.30 mm at concentration 500 mg/L. Among all extracts tested, methanol extract of the possessed moderate free radical scavenging activity with IC50 = 51.89 mg/L.

Chemical Composition, Antioxidant and Antimicrobial Activities of Essential Oils Against Pathogens Isolated from Food, Crops and Hospitals in Morocco

Chemical composition, antimicrobial and antioxidant activities of Mentha pulegium (M. pulegium) and Rosmarinus officinalis (R. officinalis) essential oils (EOs) against bacteria (Listeria monocytogenes, Pseudomonas aeruginosa and Staphylococcus aureus) isolated from food and hospitals and phytopathogenics fungi (Botrytis cinerea and Rhizopus stolonifer) isolated from crops were studied. Gas vhromatography/Mass spectrometry (GC-MS) analysis showed pulegone (86.3 %) as the major compound for M. pulegium EO (MEO) while R. officinalis EO (REO) was rich in 1,8-Cineole (35.7 %). Antibacterial activity of MEO was more important than REO. S. aureus and L. monocytogenes were the most resistant to REO while P. aeruginosa was the most sensitive to MEO. MEO was also effective against the fungal strains at low concentrations. In fact, 2 μL/mL were sufficient to inhibit 100 % of mycelium growth for both B. cinerea and R. Stolonifer. B. cinerea was more sensitive to REO than R. stolonifer. Antioxydant activity of the two EOs were examined using 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical assay method. MEO showed notable antioxidant activity when compared to BHT while REO showed a very small activity.

Antioxydant Activity of Some Algerian Olive Oil Samples in the Eastern and Center of Algeria

Antioxydant Activity of Some Algerian Olive Oil Samples in the Eastern and Center of Algeria

Phytochemical Composition and Evaluation of the Antioxidant Activity of the Ethanolic Extract of Calendula suffruticosa subsp. suffruticosa Vahl

Aim: Calendula suffruticosa ssp. suffruticosa Vahl, Medicinal and Aromatic plant, spontaneous and widespread in the Mediterranean region and in the Northeast of Algeria, is widely used by the local population for its medicinal properties. Methods: The chemical composition of the ethanolic extract of Calendula suffruticosa was established for the first time using GC / MS. The antioxidant activity of the ethanolic extract was evaluated in vitro by the DPPH (2,2-diphenyl-1-picrylhydrazyl) test, the FRAP assay and β-carotene bleaching assay. Results: sixty-four compounds are identified by GC/MS analysis, six are predominant: α-linoleic acid (24.20%), linoleic acid (23.58%), Stigmasterol (8.53%), 2-Metyl-Z-4-tetradecene (6.62%) And α-Bisabolol (1.70%). The free radical scavenging by DPPH method indicated that the ethanolic extract of C. suffruticosa showed a very high antioxydant activity with an IC50 = 0.017 ± 1.00 mg / ml. This extract also showed an ability to reduce very high iron with a maximum optical density of 1.157 at a concentration of 0.5 mg / ml and an antioxidant activity compared to the β-carotene / linoleic acid test of order of 75 ± 1.02%. Conclusion: These results show for the first time the richness of phytochemicals and the strong antioxidant activity of the species Calendula suffruticosa endemic to north-east of Algeria.

Phytochemical Screening and Biological Activities of Combretum adenogonium Leaves Extracts

Combretum adenogonium is beniniens pharmacopoeia medicinal plant used for the treatment of various diseases. This work aims to study the phytochemical and assess some biological activities of C. adenogonium leaves extracts. The phytochemical analysis (qualitative et quantitative) was conducted by standard analytical chemistry method. Antioxydant activity was evaluated by the DPPH method. Antibacterial activity was evaluated in vitro with 10 references strains , 10 Staphylococcus strains isolated from European Scientific Journal October 2017 edition Vol.13, No.30 ISSN: 1857 – 7881 (Print) e - ISSN 1857- 7431 359 meat products and 10 clinical Staphylococcus aureus strains isolated from Buruli ulcer lesions and pus. The Minimum Inhibitory Concentration (MIC) and Bactericidal (CMB) were determined by macrodilution method. The extracts cytotoxic effect was evaluated with Artemia salina larvae. The phytochemical screening revealed the presence of flavonoids, tannins, anthocyanins, saponin and triterpenoids. The methanolique extract present the higest content (450.66 ± 0.004 µg EAG/mg) of total polyphenolic compound. The results showed the good antioxidant activity. The inhibitory diameter zone vary (p < 0.001) according to the strains. The largest medium inhibitory diameter (21.85 ± 0.17 mm) was obtained with the ethanolic extract, while the lowest (6.00 ±0.00 mm) were recorded with water-ethanol extract. The variation between CMI and CMB is not significant (p > 0.05). The higher LD50 (27.66 mg/ml) was recorded with methanol extract. The results of this study confirm some use of C. Adenogonium extracts. But; these extracts must be use with moderation

Comparison of Volatile Compounds Profile and Antioxydant Activity of Allium sativum Essential Oils Extracted using Hydrodistillation, Ultrasound- Assisted and Sono-Hydrodistillation Processes

Abstract: Objective: This work aims to evaluate the impact of the use of ultrasound during essential oils extraction from garlic bulbs. Material and methods: The essential oils were extracted by Conventional Hydrodistillation method (HD), Ultrasound-Assisted hydro-distillation (US-HD) and Sono-hydrodistillation (SHD) and compared using yield profile, chemical composition and antioxidant activity. Results: Higher performances were obtained when the direct application of ultrasound were used. Indeed, SHD allowed a higher yield after only 30 min of extraction time against 70 min and 90 min for the US-HD and the HD respectively. Among the total identified compounds by GC/MS, Sulphur compounds were found to be the most abundant with qualitative similarities for the three processes. Essential oils showed a potential DPPH radical scavenging activity with IC50 values of 0.96, 1.17 and 1.23 respectively for the three HD, US-HD and SHD processes. Conclusion: The use of ultrasound for the extraction of essential oil from garlic reduced extraction time without causing significant changes in volatile composition and antioxidant activity. Keywords: Garlic essential oil, GC-MS, Antioxidant activity, Hydrodistillation, Ultrasound.