Antioxidant Properties Of Seeds Research Articles

Solvent polarity effects on extraction yield, phenolic content, and antioxidant properties of Malvaceae family seeds: a comparative study

ABSTRACT The selection of appropriate solvents in the extraction process plays a crucial role in determining the nature and quantity of secondary metabolites extracted from medicinal plants. This study investigates the impact of solvent polarity on the extraction yield, phenolic content, and antioxidant properties of seeds from four plants belonging to the Malvaceae family: Abutilon fruticosum, Abutilon pannosum, Sida ovata and Malvastrum coromendelianum. The seed powder of each species was subjected to extraction using solvents with varying polarities, including acetone, chloroform, methanol, and water. Physicochemical analysis, extraction yield, quantitative phenolic content, and antioxidant activity were assessed. Total phenolic content (TPC) was determined using the Folin–Ciocalteu reagent, and antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Analysis of variance (ANOVA) revealed that the choice of extracting solvents significantly influenced (p < 0.05) extraction yield, phenolic content, and antioxidant properties. In conclusion, water and methanol emerged as the most effective solvents for achieving high extraction yields, phenolic compound content, and free radical scavenging activities. The results demonstrated a robust correlation between total phenolic content (mg of Gallic acid equivalent/g of sample) and free radical scavenging activity (IC50). The study highlights the substantial impact of solvent type, suggesting the potential benefits of using a combination of polar and nonpolar solvents to enhance the extraction efficiency of phytochemicals, phenolic content and antioxidant quality from medicinal plant seeds. Future investigations should focus on isolating and identifying active secondary metabolites from these extracts.

Widely targeted metabolomic analysis and antioxidant properties of seeds and sprouts from different broccoli cultivars

Broccoli seeds and sprouts are popular food crops, but the nutritional values of different broccoli cultivars lack intensive research. In this study, three cultivars of broccoli seeds and their sprouts, including Lv Hua (LH), You Xiu (YX), and Bi Lv (BL), were selected to investigate the metabolites, antioxidant properties, total phenolic content (TPC), and total flavonoid content (TFC). Totally 10 categories and 1015 metabolites were identified. Comparing the sprouts of LH, YX, and BL with their respective seeds, 612, 621, and 602 differential metabolites were detected, of which 404 metabolites were shared among the three cultivars, and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the linoleic acid metabolism pathway was significant in three cultivars. Both the total and soluble fraction of LH had the highest values of antioxidant properties, TPC, and TFC. Overall, this study provided scientific support for the cultivar selection of broccoli germination and the development of their related products.

Влияние ультрафиолетового облучения на биохимические свойства растений

Depletion of the ozone layer leads to increased ultraviolet radiation, which affects the growth and functioning of plants and leads to their various physiological, biochemical, morphological and ultrastructural changes. When studying the effect of ultraviolet radiation on seed sowing qualities and biometric indicators of morphological organs of plants of various cultures, scientists did not come to a consensus on the optimal parameters of its effects. For seeds of each plant variety, there is an optimal amount of energy absorbed, resulting in a maximum effect. Ecologically significant low ultraviolet radiation changes the metabolism of reactive oxygen species and plant antioxidant systems by increasing enzyme regulation. The relevance of studies in this direction is obvious, as it allows you to stimulate the germination of seeds with physical influences, increasing their laboratory and field germination. The authors obtained and published new data on comparison of sown, crop and antioxidant properties of seeds and seedlings, seed viability, microzelenium biomass formation and change of total antioxidant activity of vegetable crops after thermodehydration. As a continuation of these studies, the purpose of this work was to study the effect of ultraviolet radiation on the biochemical properties of plants. The total antioxidant activity of sugar beet and nougat sprouts in the experiment increased under the influence of stress ultraviolet radiation. Compared to control samples (germination according to GOST in the dark), under the influence of ultraviolet radiation, antioxidant activity increases by 11.4-17.4% otn. Ultraviolet radiation is damaging factors of plant growth and development, which is manifested at different stages of ontogenesis. Thus, sowing properties are reduced (germination of sugar beet seeds is less than control by 18%, in nougat seeds by 12%), above-ground biomass at the end of the germination period is less by 49.0% in sugar beet and by 16.5% in Abyssinian nougat. Resistance to the damaging effects of ultraviolet radiation depends on the genetic nature of the plant: in the experiment, the sprouts of the Abyssinian nuga turned out to be more resistant.

Антиоксидантная активность семян и ростков сельдерейных культур

The study examined germination of seeds, antioxidant properties of seeds, sprouts and biomass of four vegetable crops under controlled conditions under various modes of seed drying. After thermal dehydration of the seeds (drying of samples at 105 °C), a sharp decrease in sowing properties occurs compared to air drying. Thermal dehydration strongly affects the viability of seeds and plants, but the level of adaptation to this stress effect is genetically determined and associated with metabolic features, forms and the state of water in plant cells and tissues. So, coriander seeds completely lose germination, and chicory seeds germinate quite well. New data are obtained on seed viability, microzelenium biomass formation and change of total antioxidant activity of studied vegetable crops under conditions of increased temperatures. In addition to chicory, carrot seeds showed some resistance to thermal dehydration. The height of the sprouts in chicory in experimental and control versions is close, while in carrots they differ almost 9 times. The yield of microselenium of carrots decreased by 11.5 times with the use of seeds subjected to thermal dehydration, the yield of chicory microselenium – by 2.7 times. The antioxy-data activity of coriander seeds, carrots and pasternaci decreases after thermodehydration, chicory seeds practically do not change. The antioxidant activity of sprouts is several times higher than that of seeds, which is characteristic of all studied crops. The formation of microzelenium during dark selection lasts no more than 2 weeks from the moment of sowing, obviously, therefore, it is impractical to grow microzelenium in the dark longer than this period.

Effect οf Genotype and Growing Year on the Nutritional, Phytochemical, and Antioxidant Properties of Industrial Hemp (Cannabis sativa L.) Seeds.

Cannabis sativa L. seeds have been an important source of protein, oil, and dietary fiber for human and animals. Currently, there is a growing interest in the commercial products of these seeds, which are recognized as a legitimate source of medicaments, cosmeceuticals, and nutraceuticals. The objective of this study was to investigate the nutritional, phytochemical composition, and antioxidant properties of seeds from seven hemp cultivars grown in Greece for three consecutive years. All the measured parameters strongly varied under the influence of growing year and genotype. In particular, protein, oil, and carbohydrates’ content of hemp seeds as well as fatty acids’ composition were mainly affected by genotype, whereas the growing year had a major effect on phytochemical components and antioxidant activity, which was determined by the 2,2′-azino-bis (3-ethylbenzthiazoline sulfonate) (ABTS) and ferric-reducing antioxidant power (FRAP) assays. Moreover, a predominant effect of the year was observed for phenolic profiles as determined by high-performance liquid chromatography and total carotenoids’ content. This study suggests that hemp seeds could be a promising food crop as a result of their high nutritive traits and antioxidant potential. A comparison of the studied cultivars, showed that Finola seeds had the highest oil and protein contents and, thus, appeared to be the most promising cultivar for cultivation in Greece.

Evaluation of phenolic compounds, antioxidant and antiproliferative activities of 31 grape cultivars with different genotypes.

This study evaluated the total phenolic content, phenolic composition, antioxidant and in vitro antiproliferative properties of seeds and skins of 31 different grape cultivars. These properties of seeds and skins varied greatly among grape cultivars. European grapes had the highest values of phenolic compounds, antioxidant properties in seeds followed by Muscadine > American grapes > Oriental grapes. European grape seed extracts also were the strongest in inhibiting the growth of HepG2 cell, followed by American grapes > Muscadine > Oriental grapes. While these values of the Euro-Asian or Euro-American hybrids fell between the parents. However, the differences of these values in skin extracts among different grape cultivars were not significant. The antiproliferative activities were significantly correlated to the three antioxidant assays and the main phenolic compounds. PRACTICAL APPLICATIONS: This study presents phenolic compounds, antioxidant, and in vitro antiproliferative properties of seeds and skins of 31 different grape cultivars. The information is highly relevant to the ever-increasing need for natural sources of antioxidants or nutraceuticals among consumers. The study also provides information for grape geneticists to breed cultivars with higher nutritional value, and for food scientists to exploit the natural polyphenol antioxidants in various grape pomace.

Phenolic compounds and the antioxidant properties in seeds of green- and yellow-podded bean (Phaseolus vulgaris L.) varieties

ABSTRACTThe objective of the article was to compare the types and quantities of phenolic compounds found in seeds of French bean from two green- and two yellow-podded cultivars. The study also comprised determinations of antioxidant properties and reducing capacity of extracts from these seeds, as well as RP-HPLC analysis of their phenolic acids. A relationship was demonstrated between the content of phenolic compounds in the seeds and the colour of their pods. Seeds of the green-podded cultivars were distinguished by a higher total content of phenolics than the yellow-podded varieties. RP-HPLC analysis of the extracts showed the presence of four phenolic acids that were identified as caffeic acid, p-coumaric acid, ferulic acid and sinapic acid. The demonstrated relationship between the seed phenolics and the pod colour can help which bean cultivars most suitable for organic cultivation and better effects on consumer health and those.Abbreviations: ABTS•+ – 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) enhancer; FRAP – reducing power of extracts; PAL – phenyl-alanine ammonia lyase; ROS – reactive oxygen species; TEAC – Trolox equivalent antioxidant capacity; TPTZ – 2,4,6-tris(2-pyridyl)-s-triazine;

Phenolic content and antioxidant properties of seeds from different grape cultivars grown in Iran

This study investigated the antioxidant activity and phenolic composition of seed extracts from three grape cultivars grown in Iran. Folin Ciocalteu method was used for the determination of the total phenolic contents and GC–MS was used for the analysis of phenolic compositions. 1,1-diphenyl-2-picrylhydrazyl (DPPH) method was used to evaluate the antioxidant activity. The highest and the lowest total phenolic contents of seed extract were found in the black and green grape, respectively. The content of individual phenols such as Frulic acid, Gentistic acid, Syringic acid, (+) Catechin, Chlorogenic acid and (−)- Epicatchin gallate was cultivars dependent. The antioxidant activity of the seed extracts ranged from 34.03% (Green) to 53.63% (Black). Generally, the Black grape seed extract with the total phenolic content (3 ± 0.01 mg tannic acid/g DM), DPPH (53.63 ± 0.34%), IC50 and AEAC (7.41 and 16.92 mg/mL) showed the highest level of total antioxidant capacity.

Lipid Profile And Antioxidant Properties of Selected Pear Cactus (Opuntia Ficus- Indica) Ecotypes From Southern Greece

The lipid profile was determined in selected ecotypes of Cactus pear (Opuntia ficus indica), and this research focused on the antioxidant properties of seeds from Cactus pear fruits. Using the methodology of methyl esters the composition of fatty acids of seeds was determined and in the case of antioxidant properties the known technique of Trolox Equivalent Antioxidant Capacity (TEAC) was used. The trolox evaluation assay was applied in aqueous - organic extracts and their residues. The seed oil composition of cactus pear fruits was studied at harvest time. Linoleic acid (70.03%) was the dominant fatty acid, followed by oleic acid (20.11%) and palmitic acid (11.86%), respectively. Among cactus pear seeds, polyphenols contribution to antioxidant properties ranged from 37.9% to 42% for TEAC values. According to the results, the seeds of Cactus pear are a good source of useful lipids and antioxidants.

Phenolic compounds and antioxidant properties of different grape cultivars grown in China

Skins and seeds of 18 grape cultivars belonging to Oriental and North American Vitis Species/hybrids, and Vitis vinifera were analysed for health beneficial properties. Four phenolic compound parameters (total phenols, flavonoids, flavan-3-ols and anthocyanins) and three antioxidant property parameters (DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, ABTS [2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid)] radical scavenging and FRAP (ferric reducing antioxidant power)) were measured. Principal component analysis (PCA) was used for this evaluation and results showed that both phenolic compounds and antioxidant properties in the seeds and skins varied among the cultivars investigated. V. vinifera “Cabernet Sauvignon” had the highest values of phenolic compounds and antioxidant properties in seeds followed by Muscadines, while the lowest appeared in the Oriental Vitis species. As expected, these values of the Euro-Asian or Euro-American hybrids fell between the parents. However, far less variation of these values was observed in the skins among different grape cultivars investigated. Interestingly, even the total phenolic contents in the berries of two cultivars are similar, distributions of phenolic compounds in seeds and skins varied greatly among them. Additionally, significant correlations among different antioxidant assays in both seeds and skins were observed. These antioxidant properties were also found highly correlated to the main phenolic compounds.