Antioxidant Properties Of Honey Research Articles

Physicochemical and Antioxidant Properties of Honey: Effect of Heat and Storage

Physicochemical and Antioxidant Properties of Honey: Effect of Heat and Storage

Physicochemical and Antioxidant Properties of Honey: Effect of Heat and Storage

Physicochemical and Antioxidant Properties of Honey: Effect of Heat and Storage

Effects of different processing methods on the antioxidant and antimicrobial properties of honey: a review

SummaryThe use of honey as a food and a medicine date back to prehistoric times. Uses include treating wounds, cuts, burns, coughs and colds. The antioxidant and antibacterial properties of honey are believed to be responsible for its medicinal properties. Honey is usually processed before consumption. Honey is subjected to thermal processing, which adversely affects its quality. Honey can also be processed by non‐thermal methods, such as high‐pressure processing, ultrasonication, irradiation and microwave processing. In this review, the effects of thermal and non‐thermal processing methods on the antioxidant and antibacterial properties of honey have been summarised. Thermal processing and microwave processing of honey adversely affect the antioxidant and antimicrobial activities of honey. In contrast to other non‐thermal processing methods, high‐pressure processing and gamma irradiation have no effect on the antibacterial activity or antioxidant properties of honey. Ultrasound processing increases antioxidant activity of honey.

Assessment of sensory properties and in vitro antimicrobial activity of monofloral Sicilian honey

The antimicrobial and antioxidant properties of honey are well-established and recently the potential of honey for biotechnological and food preservative applications appeared promising. According to that, the present study evaluated the sensory profile and the antimicrobial potential of honey samples produced in different phytogeographical zones, in the Sicilian areas, and obtained from five different floral sources (chestnut, eucalyptus, sulla, thyme, and citrus). The specific sensory fingerprint of the analyzed samples was assessed by artificial senses (E-Eye, E-Nose, and E-Tongue). In addition, the antimicrobial activity against Staphylococcus aureus ATCC 29213, Listeria monocytogenes ATCC 19114, Bacillus subtilis DSM 10, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 9027, Salmonella enterica Typhimurium ATCC 14026, and Candida albicans ATCC 10231 was evaluated. Artificial senses allowed to confirm the impact of climate factors and genetic features on honey composition suggesting implications for antimicrobial activity. A broad spectrum of antagonistic activity was displayed by the analyzed honey samples in both undiluted and diluted formulations. In particular, thyme honey showed interesting antagonistic features. In addition, a greater inhibitory effect of amber-colored honey than light ones was observed.

Radiological health risks assessment and antioxidant activities of beehive honeys: a case study of Manisa province, Turkey

ABSTRACT The physicochemical contents, antioxidant capacities, and radiological contamination levels of 20 honey samples were studied in this study to show the quality of the hive honey. Moreover, some antioxidant activities and radical scavenging properties of honey samples were investigated. The findings showed that the antioxidant properties of honey, which are strongly connected to their floral origins, have a significant therapeutic potential. While artificial radioactive 137Cs could not be detected in any of the honey samples, the average activity concentrations of natural radionuclides were 0.79 ± 0.23 Bq kg−1 for 226Ra, 0.85 ± 0.32 Bq kg−1 for 232Th and 68.33 ± 22.80 Bq kg−1 for 40K, respectively. The difference in 226Ra, 232Th, and 40K contents between honey species was not statistically significant (p>0.05). In addition, the lifetime cancer risk values calculated from honey consumption were found to be below the acceptable limit values.

Physicochemical and antioxidant properties of Coffea arabica honey from Western Oromia, Ethiopia

Coffea arabica is one of the most widely consumed and marketed commodities in the world. The study was designed to characterize C.arabica honey for botanical composition, physicochemical parameters, and antioxidant properties of honey. Twelve honey samples of C. arabica honey were collected during the flowering period of coffee flowers from the Zander hive. The physicochemical properties of honey and the Botanical origin of honey were determined based on Harmonized methods of the International Honey Commission. The antioxidant power of the coffee monofloral honey samples was determined by dissolving 1.5 gm of honey with 25 ml distilled water and mixing it with 25ml methanol and placed at 25◦C for sixty minutes of maceration using a temperature shaker. The pollen count percentage from honey indicated that all honey samples collected from Gera, Gomma, Yayu, and Manna districts were identified as coffee monofloral honey representing 84%, 93%, 75%, and 73 % of pollen count respectively. The mean moisture, ash, HMF, EC, FA, pH, fructose, glucose and sucrose content of Coffea arabica honey were 22.48%, 0.21%, 11.88, 0.49 mS/cm, 13.44 meq/Kg, 3.32, 32.77%, 32.9%, and 3.57% respectively. The total phenol and flavonoid content range from 42.1-82.1 and 21.7-59.7 mg/100 g of GAE/g respectively while the radical scavenging activity ranges from 60.2- 66.3%. The pollen analysis of honey from the area is coffee monofloral honey since its pollen count exceeds more than 45% and the honey quality also meets the Ethiopian and International standards. The antioxidant power of Coffee honey has a considerable amount of polyphenolics which have relevant antiradical activity.

Comb honey and processed honey of Croton macrostachyus and Schefflera abyssinica honey differentiated by enzymes and antioxidant properties, and botanical origin

In this study, comb and industrially processed honey samples collected from Bonga forest were investigated in relation to Melissopalynology and enzyme content, antioxidant and physicochemical properties. Melissopalynology categorized honey samples as Croton macrostachyus and Schefflera abyssinica honey. The physicochemical properties of the honey were determined using the Association of Official Analytical Chemists (AOAC) and International Honey Commission (IHC) methods. The enzymatic and antioxidant properties of honey were evaluated using the spectrophotometric method. The highest enzyme activity was observed in Croton macrostachyus comb honey (diastase = 7.44 ± 0.13°Schade and invertase = 13.97 ± 0.2 Invertase number (IN). Croton macrostachyus processed honey exhibited the highest values in flavonoids (83.36 ± 1.65 mg Catechin equivalents (CEQ)/100g)), Ferric reducing antioxidant power (FRAP) (69.94 ± 1.0 mg Ascorbic acid equivalents (AAE)/100g), and Inhibitory Concentration (IC50) (136.3 ± 0.00 mg/ml), while Schefflera abyssinica comb honey had stronger 1,1-diphenyl-2-picrylhydrazyl radical scavenging (DPPH) (49.47 ± 0.00%) activity. The principal component analysis revealed that enzymes can be associated with comb honey, and antioxidants with processed honey. Thus, comb and processed honey can be differentiated based on the enzyme level, and Croton macrostachyus and Schefflera abyssinica honey can be identified using pollen analysis.

Analysis of Antioxidant Properties and Volatile Compounds of Honey from Different Botanical and Geographical Origins

Honey has been consumed since ancient time due to its nutritional and therapeutic values. Studies showed that honey possesses antioxidant properties which can inhibit oxidation and cell damage in the body. However, the chemical contents and antioxidant properties of honeys are varied, depending on botanical and geographical origins of honey. In this study, we analysed the total phenolic content (TPC), total flavonoid content (TFC), antioxidant properties (DPPH, ABTS, FRAP and TAOC) and volatile profiles of several commercial honeys originated from Malaysia, Turkey, and Yemen. The results showed that sample H4 (Pine honey) from Turkey was the highest in TPC (0.84 µg GAE/mg honey), ABTS (63.15% inhibition) and FRAP (0.45 µg FeSO4 equivalent/mg honey) values, while H2 (Acacia honey) from Malaysia showed the highest values in TFC (0.11 µg quercetin equivalent/mg honey) and DPPH (45.13 mg/mL IC50). Meanwhile, H5 (Marai honey) from Yemen recorded the highest TAOC value (24.14 µg ascorbic acid equivalent/mg honey). Twenty-four volatile compounds were identified using gas chromatography-mass spectrometry (GC-MS), among others are 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl, linoleic acid ethyl ester, 2,5-dimethyl-4-hydroxy-3(2H)-furanone, and 2,4-dihydroxy-2,5-dimethyl-3(2H)-furan-3-one which contribute to chemical characteristics of certain honeys. In regards to the TPC, TFC, and antioxidant assays, the honey samples were ranked based on the chemical properties level as follows: H4 (Pine honey) > H2 (Acacia honey) > H7 (Kelulut 2) > H3 (Kelulut 1) > H6 (Sumar honey) > H1 (Tualang honey) > H5 (Marai honey). This finding increases the knowledge of the chemical compositions, volatile compounds, and antioxidant activities of several commercial honeys derived from different botanical and geographical origins.

Fizykochemiczna analiza miodów z rejonu Podkarpacia

Introduction. Honey is a dietary component with exceptional taste and health benefits. It is one of the most frequently counterfeit food products. Aim. The aim of the study was to assess the quality of honey on the basis of selected parameters, their compliance with standards and to determine the antioxidant properties of honey. Material and methods. The experimental material consisted of 22 samples of four types of honey: rapeseed, acacia, honeydew and multiflorous honey. The tests included the determination of water and extract content, the amount of free acids (acidity). The electrical conductivity was measured and the content of: total phenolics (TP), carotenoids (TC), and sucrose was determined. The antioxidant activity was determined spectrophotometrically using the DPPH radical and the FRAP method. Results. The total polyphenols content ranges from 0.2 mg to about 1.2 mg in 1 g of honey, carotenoid content between 4.34 to 5.26 mg/kg, in two cases, the moisture content exceeded the required value, all honeys are appropriate conductivity (except for honeydew), acidity and sucrose content. Research on the antioxidant properties of various honey varieties (DPPH and FRAP) showed the advantage of honeydew honey over other varieties. Conclusions. Most of the tested honey met the requirements of the standards. Possible deviations were minor. It can be noticed that honeys of one variety of different origin have different parameters – which proves the influence of many factors on the honey production process.

Evaluation of the changes in physicochemical and antioxidant properties of honey during storage

Background Despite having a lot of information about the physicochemical and antioxidant properties of honeys from different geographical regions, there is only limited information about the changes in these characteristics during long-term storage of honey. Hence, this study was conducted to evaluate these changes in five different types of honey, including alfalfa, milkvetch, lotus, thyme, and multifloral honeys, during one year of storage at room temperature.MethodsSamples were analyzed for pH, free acidity, Ash, moisture, electrical conductivity, hydroxymethylfurfural, color, total phenolic content, DPPH radical-scavenging activity and ferric reducing antioxidant power.ResultsChanges were more or less observed in all the physicochemical characteristics of honeys during the storage period. However, these changes did not exceed the maximum acceptable limits and after one-year of storage, the physicochemical properties of all types of honeys were within the standard limits except for HMF content in multifloral honey (43.89 mg/kg). Regarding the antioxidant capacity of honeys, our results showed 38.92-73.3 % decrease in DPPH radical scavenging activities and 43.29-67.0 % decrease in FRAP values of different types of honeys during the storage period.ConclusionFrom the nutritional point of view, decrease in the antioxidant capacity of honeys is of particular importance. Therefore, these levels of reduction in antioxidant capacity could certainly affect the nutritional and health benefits of honey.

Effect of thermal treatment on physicochemical and antioxidant properties of honey

Industrial treatments of commercial honeys during extraction and storage affect the quality of honey. One of the most common treatments in the honey industry is thermal treatment which could make some changes in the physicochemical and antioxidant properties of honey. This study was conducted to determine the effect of thermal treatment at 63 °C for 10, 20 and 30 min on physicochemical and antioxidant properties of lotus, thyme, and multifloral honeys. Samples were analyzed for pH, free acidity, moisture, hydroxymethylfurfural (HMF), color, total phenolic content, DPPH° radical-scavenging activity and ferric reducing antioxidant power (FRAP). Changes were more or less observed in all the physicochemical characteristics of honeys during the thermal treatment. However, among the physicochemical characteristics, increase in HMF content and decrease in total phenolic contents were more evident. Considering the antioxidant capacity of honeys, decreases in DPPH° radical scavenging activity of thyme honeys and FRAP values of thyme and lotus honeys during the thermal treatment were observed. Changes made in physicochemical characteristics of honeys during the thermal treatment are merely important from the standpoint of compliance with national and international legal limits. However, from the nutritional point of view, decrease in the antioxidant capacity of honeys is of particular importance and may affect the nutritional and health benefits of honey.

Melissopalynological, physicochemical and antioxidant properties of honey from West Coast of Malaysia

Stingless bees are native to tropical region and produce honey which are high in moisture content. Compared to honey from honeybees, there are limited studies on honey derived from stingless bees. Hence, the aim of this study was to evaluate the chemical composition and antioxidant activities of stingless bee honey. Fifteen types of honey were collected from six states in West Coast of Malaysia and pollen analyses were carried out. Four types of unifloral honey samples produced by stingless bees were selected to determine their physicochemical and antioxidant activities including total phenolic, total flavonoid and ascorbic acid contents. Melissopalynological study of 15 honey samples collected from different states showed presence of both unifloral and multifloral origins. Honey samples collected from Apis mellifera (honeybee) combs had lower number of total pollen compared to samples collected from Heterotrigona itama and Geniotrigona thoracica (stingless bees). Jambul Merak honey contains the highest phenolic and flavonoid contents with greatest color intensity and has the highest antioxidant potential. This study highlights the chemical composition and biological activity of honey from stingless bees which may increase its commercial value or to be utilised as potential functional food ingredient.

Evaluation of antioxygenic property of honey based on electrochemical sensing method

Antioxidant capacity of antioxidants is very important in food science. In this paper, we proposed a fast and low-cost antioxidant capacity evaluation method based on an electrochemical detection platform. Silver ions and chitosan were used to synthesize a hydrogel as the electrochemical detection platform. The depolymerization of hydrogel caused by Fenton solution can be hindered by the antioxidants. Therefore, the electrochemical signals of silver ions can be used to measure the antioxidant properties of honey. Jujube honey, linden honey, locust honey and wattle honey were submitted to evaluation. Results indicates the antioxidant capacities of the four honeys follows following order: Jujube honey > linden honey > locust honey > wattle honey.

Investigating 5-hydroxymethylfurfural formation kinetic and antioxidant activity in heat treated honey from different floral sources

The quality and biochemical properties of honey are affected by heating or during storage period. The most important biochemical reaction that occurs in this process is the Maillard reaction. HMF (5-hydroxymethylfurfural) is one of the major intermediate products in the Maillard reaction that can lead to quality reduction in heated honey. In this study, the effect of heating on the antioxidant activity, and colour values as Maillard reaction indicators of three different botanical honeys were investigated; the HMF formation was also determined. Temperatures of 50, 70 and 80 °C were applied on the honeys between 0 and 48 h. Total phenolic content, ferric reducing/antioxidant power and scavenging of 2,2-diphenyl-1-picrylhydrazyl free radical assays are used to determine the antioxidant capacity. Results showed that the formation of HMF and the antioxidant properties of honeys were significantly increased during the heating process. Pure HMF compound also showed lower antioxidant activity. The formation of HMF has higher degree of linearity in the fit of the zero order reaction and also it was the highest in the chestnut honey. Furthermore, it was found that as the biochemical value of the honeys increased, the HMF formation decelerated.

Phenolic compounds, antioxidant capacity and physicochemical properties of Brazilian Apis mellifera honeys

Forty-nine honey samples from five monofloral (eucalyptus, mastic, wildflower, Japanese grape and quitoco) and six polyfloral honeys were analyzed for their physicochemical and antioxidant compounds. Levels of total phenolics and flavonoids as well as individual composition were analyzed by HPLC. The oxygen radical absorption capacity (ORAC), the ferric reducing antioxidant power (FRAP), and the 2,2-diphenyl-1-picrylhydrazyl free radical assays (DPPH) were performed to determine antioxidant capacity. The highest antioxidant capacity was observed in eucalyptus and mastic honeys; gallic acid levels were greatest in both of them while p-coumaric acid was highest in Japanese grape honey. Physicochemical and antioxidant properties of honey were found to be dependent on its botanical origin. Linear discriminant analysis was able to differentiate 90% of honeys in terms of the seasons when they were collected and the main discriminant responses were p-coumaric acid, titratable acidity, diastase activity, and total flavonoids content. LDA correctly classified 96% of honeys according to their botanical origin.

Physicochemical and Antioxidant Properties of Honeys from the Sundarbans Mangrove Forest of Bangladesh.

This study evaluated the physicochemical, nutritional, antioxidant, and phenolic properties of ten honey samples from the Sundarbans mangrove forest, Bangladesh. The average pH, electrical conductivity, total dissolved solid, ash, moisture, hydroxymethyl furfural, titrable acidity, and absorbance were 4.3, 0.38 mS/cm, 187.5 ppm, 0.14%, 17.88%, 4.4 mg/kg, 37.7 meq/kg, and 483 mAU, respectively. In the honeys, the average contents of Ca, Cu, Fe, K, Mg, Mn, and Na were 95.5, 0.19, 6.4, 302, 39.9, 3.4, and 597 ppm, respectively, whereas Cd, Cr, Pb, and Ni were not found. The average contents of total sugar, protein, lipid, vitamin C, polyphenols, flavonoids, and anthocyanins in the honeys were 69.3%, 0.8%, 0.29%, 107.3 mg/kg, 757.2 mg gallic acid equivalent/kg, 43.1 mg chatechin equivalent/kg, and 5.4 mg/kg, respectively. The honeys had strong 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity, reducing power and total antioxidant capacity. High-performance liquid chromatography analysis of the honey fractions revealed the quantification of six polyphenols namely, (+)-catechin, (−)-epicatechin, p-caumeric acid, syringic acid, trans-cinnamic acid, and vanillic acid at 194.98, 330.34, 74.64, 218.97, 49.55, and 118.84 mg/kg, respectively. Therefore, the honeys in the Sundarbans are of excellent quality and a prospective source of polyphenols, and antioxidants.

Influence of the Addition of Selected Spices on Sensory Quality and Biological Activity of Honey

Bee honey is nutritious and has numerous health benefits, but its taste is for many people too bland. Honey with addition of spices could be important to the food industry as a functional product with positive health image and interesting taste. Such product would definitely meet health-driven consumers’ expectations. Therefore, the aim of this study was to evaluate the effect of addition of selected spices on sensory, antimicrobial, and antioxidant properties of honey. Results showed that the addition of spices significantly affected the taste and the smell of honey (p<0,05) and that honey with the cinnamon was the most desired and easily accepted product by the consumers. The addition of spices had no significant effect on texture and appearance. All tested samples showed the ability to inhibit the growth of bacteria and, contrary to the assumptions, the addition of spices did not cause an increase of antimicrobial activity. The results also showed that the kind and amount of added spice significantly affected the antioxidant activity: ability to scavenge free radicals and total phenolics content. The highest antioxidant activity revealed the honey with cinnamon and the lowest revealed the honey with cardamom addition.

The Use of the PHOTOCHEM Device in Evaluation of Antioxidant Activity of Polish Honey

Forty samples of varietal Polish honeys were investigated in order to assess their antioxidant capacity by photochemiluminescence (PCL) methods using standard ACW and ACL kits. The reference methods, FRAP and DPPH, were used. The total phenolics content (TPC) by Folin-Ciocalteau method was determined. The antioxidant properties of honeys as well as TPC were mainly dependent on honey botanical origin. The highest phenolic content and antioxidant activity for dark honeys, while the lowest for white honeys, were found. Moreover, the results obtained by used analytical methods were comparable and the best correlation between PCL and FRAP (r > 0.81) and DPPH (r = 0.74) methods was observed. The TPC in honeys was positively correlated with its antioxidant activity measured by PCL (r > 0.76). Used for the first time, PCL assay demonstrated usefulness in determining the antioxidant capacity of honey, divided into water-and lipid-soluble fractions, measured by ACW and ACL kits, respectively.

Comparative Analysis of the Physico-chemical and Antioxidant Properties of Honey Available in Tangail, Bangladesh

The aim of the current study was the comparative analysis of the physical, biochemical and antioxidant properties of Mustard, Litchi, and Nigella honey. The physical parameters include the mean moisture content of Mustard, Litchi and Nigella honey were found 14.32%, 15.27%, and 14.12% respectively; P H content were 6.97, 5.8, 4.78; the TSS content were71%, 70%, and 73% respectively. The biochemical parameters including protein content were 1.103%, 0.57% and 0.985%; mean ash content were 0.2%, 0.19%, and0.188%, total carbohydrate content were 84.377%, 83.97% and 84.496% and the minerals contents Na were 539.238 ppm, 424.3565 ppm and 588 ppm; K were 239.216 ppm, 219.017 ppm and1063.774 ppm; Ca were 1710.499 ppm, 611.4852 ppm and 75.4 ppm for the Mustard, Litchi and Nigella honey respectively. The Antioxidant properties such as polyphenol content were found 42.572 ± 0.00529 mg gallic acid/50 ml, 30.625 ± 0.00529 mg gallic acid/50 ml and 95.5 ± 0.00529 mg gallic acid/50 ml; flavonoid content were found 1.052 ± 0.000577 mg catechin/50 ml, 0.6 ± 0.000577 mg catechin/50 ml and 2.66 ± 0.000577 mg catechin/50 ml and vitamin C content were 0.41 mg/100ml, 0.63 mg/100ml and 0.7 mg/100ml for Mustard, Litchi and Nigella honey respectively.

Honey and its Anti-Inflammatory, Anti-Bacterial and Anti-Oxidant Properties

Honey mainly consists of sugars and water. Apart from sugars, honey also contains several vitamins, especially B complex and vitamin C, together with a lot of minerals. Some of the vitamins found in honey include ascorbic acid, pantothenic acid, niacin and riboflavin; while minerals such as calcium, copper, iron, magnesium, manganese, phosphorus, potassium and zinc are also present. Honey has been used for its healing, nutritional and therapeutic properties since ancient times. Its antibacterial potentials even against multi-drug resistant bacteria, such as Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacterbaumanni have been proved. Honey is well known for its anti-inflammatory and antioxidant capacities, which may be useful for the prevention of chronic inflammatory process like atherosclerosis, diabetes mellitus and cardiovascular diseases. The antibacterial, antiinflammatory and antioxidant properties of honey will be reviewed here.