Fruit Antioxidant Compounds Research Articles

The Potential Roles of the N and P Supplies on the Internal Browning Incidence in Sweet Cherries in the Southern Chile

Southern Chile has experienced a strong increase in sweet cherry production in recent years. However, there are climatic gaps that negatively reduce the fruit quality and yield of exportable fruit, such as the high incidence of rains during flowering and pre-harvest. The use of roof covers has become an agronomic solution that offers protection from weather events, such as rain, which will significantly increase the exportable fruit. However, the use of plastic covers can cause negative impacts on the fruit, such as a loss of firmness and acidity. Currently, the incidence of internal (pulp) browning has reduced the quality of cherries produced in Southern Chile, although research on this subject is largely under explored. Some studies reported that a high content of antioxidants in the fruit, both phenolic and non-phenolic (e.g., ascorbic acid), could reduce the incidence of the physiological disorder of browning. The soils of Southern Chile are characterized by the high content of organic matter, which implies high levels of available nitrogen (N) and a high phosphorus (P) content. Some studies, however, have shown that fertilization with N and P would significantly improve the postharvest quality, shelf life, and the accumulation of antioxidant compounds in fruits, even better than other strategies, including selenium and chitosan applications. However, there needs to be more detailed information on this aspect of the sweet cherry fruit production. The quality attributes and postharvest life of cherries are closely associated with the antioxidant levels of fruits, which could be related to either the soil acidity level of the Chilean Andisols or the levels of P and N in soil or plant tissues. Therefore, the objective of this review was to discuss the role of the N and P supply on the internal browning incidence in sweet cherries and relate it to what is known in other fruits.

Contents of nitrogen compounds during bud break and peach tree performance in response to budburst-inducing products

The growth of peach orchards in subtropical regions depends on the minimal requirements for chilling accumulation, and crop loading requires sprays of chemical compounds to overcome dormancy, induce bud break, and allow adequate flowering and fruiting. The ability of hydrogen cyanamide, nitrogen fertilizer and calcium nitrate to break the dormancy of ‘BRS Kampai’ peach trees and their effects on nitrogen, tryptophan, and polyamine contents in buds were evaluated. Furthermore, budburst, flowering, fruit set percentage, flowering period, tree performance, and bioactive compound content in the fruits were assessed. The study was undertaken in São Paulo state, a subtropical region of southeast Brazil, and the field experiment was carried out in two crop seasons. Short flowering duration and higher percentages of budburst, flowering, and fruit setting were correlated with higher nitrogen content in vegetative buds and spermidine contents in flower buds. The better performance of hydrogen cynamide, compared with other treatments, was confirmed by the anticipation, patterning, and increase in budburst, flowering, and fruit setting, associated with high levels of spermidine in the flower buds. Hydrogen cyanamide also increases the concentration of antioxidant compounds in fruits. Hydrogen cyanamide and the combination of nitrogen fertilizer and calcium nitrate enhanced yield and production efficiency and are recommended for inducing budburst in subtropical regions.

Italian Tomato Cultivars under Drought Stress Show Different Content of Bioactives in Pulp and Peel of Fruits.

Background: This study aims to evaluate the performance, in terms of accumulation of antioxidant compounds in fruits, of nine local and three commercial Italian tomato cultivars subjected to drought stress. The same local cultivars had been previously studied at morpho-physiological level. Methods: The present manuscript analyzes drought stress as a tool to increase the amount of secondary metabolites that can enhance fruit quality. Nutraceutical characterization of the fruits was performed by analyzing the content of antioxidants, phenols, flavonoids, lycopene, ascorbic acid (vitamin C), rutin, caffeic acid, and naringenin. At the same time, plant sensitivity to stress during the reproductive phase was monitored in terms of flower abscission, fruit drop, and seed germination. Results: Perina turns out to be the tomato cultivar with the best nutraceutical properties in the absence of stress while the Quarantino cultivar is so for flavonoid content (control plants) and lycopene and vitamin C content (stressed plants). Perina and Quarantino are the cultivars with the best response to drought and Perina has the highest concentrations of bioactives. Quarantino responds most effectively to stress in the reproductive phase. Conclusions: data confirm that drought stress increases bioactive production in some local cultivars of tomato, which produce higher quality fruits.

The Antioxidant Profile Evaluation of Some Tomato Landraces with Soil Salinity Tolerance Correlated with High Nutraceuticaland Functional Value

Romania has a wide variety of local landraces and heirloom genotypes. Our study aims to assess the performance of twenty halotolerant tomato landraces, collected from areas with medium and high levels of soil salinity, in terms ofthe accumulation of antioxidant compounds in fruits and to cluster them according to their nutraceutical components. The tomatoes used in the study were harvested once they had attained full ripeness and then analyzed for lycopene (Lyc), ascorbic acid content (AsA), total phenolic content (TPC), and total antioxidant capacity (TAC). The results revealed major differences between genotypes in terms of nutraceutical values. According to principal component analysis, the tomato landraces were grouped into five clusters, characterized by different proportions of compounds with antioxidant activity. The high/moderate nutritional values of Lyc, TAC, TPC, and AsA were obtained from varieties taken from local lands with high soil salinity, over 6.5 dS m−1. These findings support the idea that metabolites and secondary antioxidants are involved in the process of stress adaptation, thereby increasing salinity tolerance in tomatoes. Our results show that there are tomato landraces with a tolerance of adaptation to conditions of high soil salinity and provide information on their ability to synthesize molecules with antioxidant functions that protect plants against oxidative damage.

Ameliorative effects of calcium nitrate and humic acid on the growth, yield component and biochemical attribute of pepper ( Capsicum annuum ) plants grown under salt stress

The present work was carried out in order to determine the effects of calcium nitrate and humic acid applications either separately or in combination on the growth and fruit yield quality of pepper plants under salt stress condition. Two different concentrations of calcium nitrate (Ca1; 60 mg/kg soil and Ca2; 120 mg/kg soil) were applied to the soil before sowing whereas humic acid applications (HA1; 750 mg/kg soil and HA2; 1500 mg/kg soil) were performed during plant growth (at the third true leaves stage). Plants were irrigated with saline water in a concentration of 100 mM NaCl. Data showed that applications of both concentrations of humic acid and lower concentration of calcium nitrate (Ca1) individually caused significant increases in growth parameters, RWC, photosynthetic pigments, minerals content, non-enzymatic antioxidants contents of the plants under normal and salt stress conditions. Moreover, fruit antioxidant compounds and quality of fruits (capsaicin, lycopene, β-carotene, total phenol, total flavonoids and antioxidant activity) were improved by using theses treatments. The combined treatment of Ca1 and HA2 was the most effective one on the previous criteria under salt stress conditions. Therefore, the usage of humic acid and calcium could be suggested to improve the soil properties, growth and antioxidant capacity of pepper plants and to mitigate the damage caused by salt stress.

Feeding on ripening and over-ripening fruit: interactions between sugar, ethanol and polyphenol contents in a tropical butterfly

In ripe fruit, energy mostly derives from sugar, while in over-ripe fruit, it also comes from ethanol. Such ripeness differences may alter the fitness benefits associated with frugivory if animals are unable to degrade ethanol when consuming over-ripe fruit. In the tropical butterfly Bicyclus anynana, we found that females consuming isocaloric solutions mimicking ripe (20% sucrose) and over-ripe fruit (10% sucrose, 7% ethanol) of the palm Astrocaryum standleyanum exhibited higher fecundity than females consuming a solution mimicking unripe fruit (10% sucrose). Moreover, relative to butterflies consuming a solution mimicking unripe fruit, survival was enhanced when butterflies consumed a solution mimicking either ripe fruit supplemented with polyphenols (fruit antioxidant compounds) or over-ripe fruit devoid of polyphenols. This suggests that (1) butterflies have evolved tolerance mechanisms to derive the same reproductive benefits from ethanol and sugar, and (2) polyphenols may regulate the allocation of sugar and ethanol to maintenance mechanisms. However, variation in fitness owing to the composition of feeding solutions was not paralleled by corresponding physiological changes (alcohol dehydrogenase activity, oxidative status) in butterflies. The fitness proxies and physiological parameters that we measured therefore appear to reflect distinct biological pathways. Overall, our results highlight that the energy content of fruit primarily affects the fecundity of B. anynana butterflies, while the effects of fruit consumption on survival are more complex and vary depending on ripening stage and polyphenol presence. The actual underlying physiological mechanisms linking fruit ripeness and fitness components remain to be clarified.

Effect of vermicompost on fruit yield and quality of bell pepper

In this study effect of application of food waste vermicomposts to soil on antioxidant compounds, fruit yield and quality of sweet pepper (Capsicum annum L.) were investigated in field condition. Four vermicompost levels (0, 5, 10 and 15 t/ha) were applied to soil based on a randomized complete block design with three replications. The results showed that the highest (21.87 kg m2)and lowest (14.69 kg m2)fruit yield were achieved in plants treated with 5 t/ha vermicompost and control, respectively.Vermicompost treatments positively influenced fruit antioxidant compounds (antioxidant activity, total phenolic, carbohydrate content and total flavonoid). The highest antioxidant activity (81%) and carbohydrate content were obtained in plants treated with 10 t/ha vermicompost, while their lowest values were recorded in the control plants. Fruit quality indices (pH, titratable acidity, ascorbic acid and fruit firmness) were significantly influenced by vermicompost treatments. However, no significant difference was found for total soluble solids between treatments. There were 40, 61 and 56% increase in the amount of Titratable acidity, ascorbic acid content and fruit firmness following application of vermicompost (15 t/ha) when compared to their values in control, respectively. In conclusion, soil application of vermicompost can positively influence antioxidant compounds, fruit yield and quality of pepper.

Effect of compost on antioxidant components and fruit quality of sweet pepper (capsicum annuum L.)

In order to determine the effect of compost (CO) on antioxidant compounds and fruit quality of sweet pepper (Capsicum annum L.), an experiment was conducted in open field. Treatments consisted of four levels of compost (0, 5, 10 and 15 ton ha-1).The experiment was designed in randomized block design with three replications. Compost treatments positively affected fruit antioxidant compounds of pepper (antioxidant activity, total phenolic and carbohydrate content).But, no significant difference was found in total flavonoid content between compost and control treatments. The highest antioxidant activity and carbohydrate content were obtained in plants treated with10 ton ha-1 of compost. Fruit quality factors (pH, total soluble solids, titratable acidity, ascorbic acid and fruit firmness) were influenced by compost treatments. Total soluble solids, and fruit firmness significantly increased in response to compost treatments and the highest values were obtained from the most level of compost treatment (15 t ha-1). Thus, these results showed that compost has strong impact on fruit quality and antioxidant compounds of pepper plants under field conditions.

Effect of compost on antioxidant components and fruit quality of sweet pepper (Capsicum annuum L.).

In order to determine the effect of compost (CO) on antioxidant compounds and fruit quality of sweet pepper (Capsicum annum L.), an experiment was conducted in open field. Treatments consisted of four levels of compost (0, 5, 10 and 15 ton ha-1).The experiment was designed in randomized block design with three replications. Compost treatments positively affected fruit antioxidant compounds of pepper (antioxidant activity, total phenolic and carbohydrate content).But, no significant difference was found in total flavonoid content between compost and control treatments. The highest antioxidant activity and carbohydrate content were obtained in plants treated with10 ton ha-1 of compost. Fruit quality factors (pH, total soluble solids, titratable acidity, ascorbic acid and fruit firmness) were influenced by compost treatments. Total soluble solids, and fruit firmness significantly increased in response to compost treatments and the highest values were obtained from the most level of compost treatment (15 t ha-1). Thus, these results showed that compost has strong impact on fruit quality and antioxidant compounds of pepper plants under field conditions.

Quantitative trait loci pyramiding for fruit quality traits in tomato

Fruit quality is a major focus for most conventional and innovative tomato breeding strategies, with particular attention being paid to fruit antioxidant compounds. Tomatoes represent a major contribution to dietary nutrition worldwide and a reservoir of diverse antioxidant molecules. In a previous study, we identified two Solanum pennellii introgression lines (IL7-3 and IL12-4) harbouring quantitative trait loci (QTL) that increase the content of ascorbic acid (AsA), phenols and soluble solids (degrees Brix; °Bx) in tomato fruit. The purpose of the present work was to pyramid into cultivated varieties the selected QTL for enhanced antioxidant and °Bx content. To better understand the genetic architecture of each QTL, the two ILs were crossed to the recurrent parent M82 (ILH7-3 and ILH12-4) and between them (ILH7-3+12-4). F1 hybrids (ILH7-3+12-4) were then selfed up to obtain F3 progenies in order to stabilize the favourable traits at the homozygous condition. Species-specific molecular markers were identified for each introgressed region and allowed us to select four F2 genotypes carrying both introgressions at the homozygous condition. The F3 double homozygous plants displayed AsA, total phenols and °Bx content significantly higher than M82. Therefore, they may represent suitable genetic material for breeding schemes aiming to increase antioxidant content in tomato fruit.

Characterisation of antioxidant compounds in sweet bell pepper (Capsicum annuum L.) under organic and conventional growing systems

Sweet red bell pepper is one of the best sources of ascorbic acid and carotenoids as well as phenolic compounds important in the human diet. There have been some studies showing a higher level of bioactive compounds in organic bell pepper fruits compared with conventional fruits, but not all studies have been consistent in this respect. The levels of carotenoids and phenolics are very variable and may be affected by ripeness, genotype and cultivation. The results obtained in this study showed that an organic growing system affected the level of bioactive compounds (carotenoids and polyphenols) in sweet bell pepper fruits cultivated in Poland. Organic bell pepper fruits contained significantly more dry matter, vitamin C, total carotenoids, β-carotene, α-carotene, cis-β-carotene, total phenolic acids (as well as individual gallic and chlorogenic acids) and flavonoids (quercetin D-glucoside, quercetin and kaempferol) compared with conventional fruits. The bell pepper variety also affected the level of antioxidant compounds in fruits. Organic growing increased the level of antioxidant compounds such as carotenoids, phenolic compounds and vitamin C in sweet bell pepper. The second significant factor affecting the antioxidant compound content of sweet bell pepper was variety. It would be necessary to continue this study as a long-term experiment in order to eliminate the influence of seasonality.

Antioxidant compounds in fruits and vegetables and changes during postharvest storage and processing

Antioxidant compounds in fruits and vegetables and changes during postharvest storage and processing

Flavonoid Profiling and Biosynthetic Gene Expression in Flesh and Peel of Two Tomato Genotypes Grown under UV-B-Depleted Conditions during Ripening

The effect of shielding solar ultraviolet B radiation on the accumulation of some flavonoids and their precursor hydroxycinnamic acids in tomato (Solanum lycopersicum) was evaluated by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In particular, flesh and peel of two tomato hybrids, DRW 5981 and Esperanza, were separately analyzed. The hybrids have been chosen for their different responses to the light, since it was previously reported that they show different pigmentation and opposite behavior under UV-B in terms of carotenoids and ascorbic acid content at different ripening stages. To determine the effect of UV-B radiation during tomato ripening, we also measured the expression of some flavonoid biosynthetic genes by real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis. The results allowed us to conclude that UV-B radiation deeply and differentially affects the content of the considered flavonoids and hydroxycinnamic acids as well as the expression of some of their biosynthetic genes in both flesh and peel during the ripening process. On the other hand, the collected data clearly showed that this influence varies between different genotypes. We conclude that the use of specific plastic covers able to eliminate UV-B radiation may be an environmentally friendly approach to modulate the expression of structural genes and, in turn, to enhance healthy antioxidant compounds in fruits of specific tomato cultivars.

Electron-Beam Ionizing Radiation Stress Effects on Mango Fruit (Mangifera indica L.) Antioxidant Constituents before and during Postharvest Storage

The effect of electron-beam ionizing radiation stress and storage on mango fruit antioxidant compounds was evaluated in a dose range of 1-3.1 kGy. Phenolic high-performance liquid chromatography (HPLC) profiles were not affected right after the irradiation process; however, an increase in flavonol constituents was observed after 18 days in storage (3.1 kGy). Total phenolics by the Folin Ciocalteu method and antioxidant capacity (ORAC) were not affected, while reduced ascorbic acid decreased approximately 50-54% during storage (>/=1.5 kGy). No major changes in carotenoid HPLC profiles indicated a delay in ripening of irradiated mangoes (1-3.1 kGy) compared to nonirradiated fruits. However, irradiation dose >/=1.5 kGy induced flesh pitting due to localized tissue death. A summary of the potential roles of reactive oxygen species generated by the irradiation stress on different antioxidant constituents of mango fruits is presented.

17] Antioxidant activity by headspace gas chromatography of volatile oxidation products of ω-6 and ω-3 polyunsaturated lipids

Publisher Summary This chapter describes the antioxidant activity by headspace gas chromatography (GC) of volatile oxidation products of ω-6 and ω-3 polyunsaturated lipids. The technique of headspace GC is rapid and suitable for biological materials because no sample workup is necessary. This method can be used to determine the specific oxidation of ω-3 and ω-6 polyunsaturated fatty acids (PUFA). It is ideal for the measurement of oxidative susceptibility as affected by diet and etiology of biological samples. This method may prove to be valuable for routine checking of antioxidant supplementation and clinical etiology. A wide range of phenolic compounds have been shown to inhibit the in vitro oxidation of human low-density lipoproteins (LDL), which plays an important role in the initiation of atherosclerosis. Plant phenolic antioxidants in fruits and vegetables may also have a protective effect on coronary heart disease and cancer, but the molecular basis of protection is not understood. A better knowledge of the mechanism of natural antioxidants requires more systematic studies with specific methods providing specific chemical information that can be related directly to oxidative modifications of biological systems. Pharmacokinetic data are needed to evaluate the effectiveness of the polyphenolic antioxidant compounds in fruits and beverages and their potential role in reducing coronary heart disease.