Antioxidant Activity Of Pulp Research Articles

Study on Biochemical Composition of Sea Buckthorn Fruit Cultivated from Leh, India and its Flavor Profile by Gas Chromatography Mass Spectrometry (GC-MS)

Sea buckthorn berries are among the most nutritious and vitamin rich fruit found in the plant kingdom. Biochemical composition of sea buckthorn berries cultivated from Leh, India was analyzed to study its suitability for making food products. Berry pulp was analyzed for sugars, acidity, TSS and pH. The results show that the sea buckthorn pulp exhibited higher acidity, lesser pH, total sugars and reducing sugars, when compared to fruits like pineapple and grapes. Calcium and potassium content of berry was found to be high when compared to other mineral content viz. sodium, iron and phosphorus. Antioxidant contents and antioxidant activity of pulp was determined and compared with other fruit pulps. Sea buckthorn fruit pulp exhibited significant antioxidant activity i.e. 88.72%, when compared to locally available fruits like grapes and pineapple. Totally 45 volatile flavour components were identified from sea buckthorn berry, which contains mainly esters, that contributes to significant berry aroma.

Panicle and Fruit Characteristics Influenced by Plant Age In Litchi cv. Rose Scented

An experiment was conducted during the cropping season of 2018 and 2019 to determine how plant ages affect flowering, fruit quality and incidence of sunburn and fruit cracking in litchi cv. Rose Scented. In a lot consisting of litchi plants aged between 1 and 30 years, 25-30- years-older plants initiated panicle emergence earlier with larger-sized panicles (42.35 cm length and 28.65 cm spread) and thicker peduncle (>6 mm). Fruit retention per panicle was also more in number (15.68) in 25-30- years older plants as compared to 1-5 year younger plants (4.76). An important observation involved fruits being borne closer to leaf in 25-30- years-older plants, which ultimately resulted in lesser incidence of sun burn and fruit cracking. Older plants (25-30-years) produced bigger fruit than 1-5 year-younger ones and highest fruit weight (22.58g) was recorded in 15–20-year-old plant. The time taken for fruit borne in older trees (25-30-years) to attain optimum fruit maturity was extended or delayed by 5 days as compared to younger plants (1-5-year). Total soluble solids was also higher in fruits borne on 25-30 years older litchi plants, while titratable acidity and ascorbic acid contents was more in fruits borne on 1-5 year younger plants. Total phenolics and antioxidant activity of pulp were the highest in young plants and least in older plants. we conclude that heavy bunches and proximity of fruit to leaf are important traits in litchi for consideration in breeding programmes to identify clones or variants tolerant to sun burn and fruit cracking. Bangladesh J. Bot. 53(2): 279-285, 2024 (June)

Assessment of lipid profile and antioxidant activity of pulp, shell, and kernel of Moroccan Balanites aegyptiaca fruit

Assessment of lipid profile and antioxidant activity of pulp, shell, and kernel of Moroccan <i>Balanites aegyptiaca</i> fruit

Physical-chemical characterization, bioactive compounds, and antioxidant activity of pulp and peel of the Jamelão

The high production of jamelão (Syzygium cumini) during harvest and the lack of information about it emphasize the development of new processing technologies for this fruit since it has been proven that its different parts have functional properties. The objectives of this work were to evaluate the physical-chemical characteristics and to determine the content of bioactive compounds and the antioxidant activity present in the pulp and peel of the jamelão in order to assess which one has the greatest industrial potential. For this, the fruit was pulped and separated into peel and pulp. The peel was subsequently dehydrated, resulting in three samples: pulp, fresh peel, and dried peel. It was observed that drying the peel provided an increase in the values of ash, lipids, acidity, and soluble solids to a higher or equal level to those of the pulp. In addition, total phenolic compounds, total flavonoids, total monomeric anthocyanins, and total antioxidant activity of the fresh peel were found to be ten times higher than those of the pulp. Thus, the peel, a by-product of jamelão, has great potential to be used in the food, pharmaceutical and cosmetic industries.

Extraction of Carotenoids from Pumpkin Peel and Pulp: Comparison between Innovative Green Extraction Technologies (Ultrasonic and Microwave-Assisted Extractions Using Corn Oil).

Natural pigments improve aesthetic value as well as antioxidant potential of a food product. This study was designed to determine the effects of green extraction techniques on carotenoids, polyphenols and antioxidant activities of pulp and peel of two varieties of pumpkin (Cucurbita maxima). Innovative green extractions (IGE; Ultrasound and Microwave-Assisted Extractions) synergised with corn oil (used as green solvent) were compared with conventional extraction (CE; hexane/isopropyl alcohol; 60:40, v/v). Results showed total carotenoids to be almost double on employing IGE (PM2-UAE-peel = 38.03 ± 4.21; PM4-UAE-peel = 33.78 ± 1.76 µg/g) when compared to conventional extraction (PM2-CE-peel = 19.21 ± 4.39; PM4-CE-peel = 16.21 ± 2.52 µg/g). Polyphenolic contents ranged between 510.69 ± 5.50 and 588.68 ± 7.26 mg GAE/100 g of extract in IGE, compared with conventional extracts (269.50 ± 2.17 to 318.46 ± 6.60 mg GAE/100 g) and percent inhibition of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) ranging between 88.32 ± 1.51 and 93.53 ± 0.30% in IGE when compared with conventional extraction (50.61 ± 1.44 to 57.79 ± 2.09%). Further, oxidative stability of carotenoids extracts from IGE (protection factor = 1.59 ± 0.01 to 1.81 ± 0.05) were found to be significantly higher (p < 0.05) than conventional extracts. Based on results, this study supports the use of innovative green extraction techniques to obtain bioactive pigments like carotenoids. It is anticipated that results generated will find potential applications in food, pharmaceutical and cosmetic industries.

Antibacterial and antioxidant activity of pulp, peel and leaves of Feijoa sellowiana: Effect of extraction techniques, solvents and concentration

The present study evaluated the effect of extraction techniques (ultrasound-assisted extraction (U) and shaking water bath extrac-tion (WB)), solvents (etha-nol, acetone and distilled water) and concentration (2.5% and 5%) on total phe-nolic content (TPC), anti-bacterial and antioxidant activities of extracts ob-tained from feijoa leaves, peel and pulp. The antibac-terial activity of feijoa ex-tracts were tested in vitro against 6 pathogens bacteria by the disc diffusion method and the antioxidant activity was evaluated by 2,2 diphe-nyl-1-picrylhydrazyl radical assay. The results indicated that leaves and peel extracts exhibited stronger antibac-terial activity than that of pulp. In generally, WB-A5%, WB-W5%, U-A5% and U-W 5% extracted with acetone (A) and water (W) at 5% concentration from feijoa leaves, peel and pulp showed more antibacterial activity against all tested pathogen bacteria. The leaves, peel, and pulp ex-tracts had high antioxidant activity with 85.78-90.82%, 89.86-91.60%, and 81.49-91.31%, respectively. Peel extracts had slightly higher antioxidant activity than leaves and pulp extracts. TPC of leaves, peel, and pulp extracts were in the range of 488.99-554.00, 349.17-517, and 115.64-345.46 mg gallic acid equivalents (GAE)/100 g of extract. The overall findings suggested that different part of feijoa (especially leaves and peel) could be used as a natural antibacterial and an-tioxidant for functional foods.

Physicochemical characterization, bioactive compounds and antioxidant activity of pulp, peel, endocarp and food paste developed with buriti pulp and waste (&lt;i&gt;Mauritia flexuosa&lt;/i&gt; L.)

Buriti (Mauritia flexuosa L) is an Amazonian fruit with high potential to be used as a source of bioactive compounds and healthy food preparations due to its attractive yellow / orange color. The purpose of this paper was to verify the physicochemical characteristics, bioactive compounds and antioxidant activity in pulp, endocarp, peels, and three food paste developed with Buriti pulp and waste. In addition to the centesimal composition of the fractions of the fruit and the preparations, analyzes of vitamin C, phenolic compounds, carotenoids and antioxidant activity were also carried out. The following values were observed for the fractions of the fruit: vitamin C (48.44-55.22 mg/100 g), carotenoids (6.05-21.03 mg/100g), Total phenolics (19.31-33.30 mg GAEq/100g) and antioxidant activity (111.24-190.43 µmol TE/g DM), for all analyzed parameters, the highest values were observed for the peel. As for the formulations, it was found that the good attributes of the peel interfered in the formulation C, which presented the highest yields of vitamin C (21.22 mg/100g), carotenoids (13.99 mg/100g), Total phenolics (21.45 mg GAEq/100g) and antioxidant activity (82.34-130.52 µmol TE/g DM). Thus, it is verified that buriti has a great nutritional potential for direct consumption or to be used in the formulation of confectionery products, including the use of its peels in formulations.

Proximate composition and antioxidant activity of Syzygium cumini fruit grown at different regions in Bangladesh

The risk of degenerative diseases decreases with the consumption of high antioxidant containing fruits. This study aimed to analyze the proximate composition and antioxidant activity of pulp and seed of Syzygium cumini. Proximate composition was determined by following the AOAC method. Minerals and heavy metals were analyzed using a flame photometer and an atomic absorption spectrometer. In-vitro antioxidant activity was evaluated by using phosphomolybdenum, ferrous ion chelating ability assay and reducing power assay methods. In proximate composition analysis pulp contained significantly (p&lt;0.05) greater amount of minerals than seed but seed contained a higher amount of protein and lipid than pulp. In the bioactive compound analysis, seed extracts possessed significantly (p&lt;0.05) greater amount of Chlorophyll A, Chlorophyll B and total carotenoids. Additionally, seed extracts exhibited significantly (p&lt;0.05) more total antioxidant capacity, reducing power and ferrous ion chelating ability compared to pulp extracts. This study revealed that the seed of Syzygium cumini contains significantly (p&lt;0.05) higher amount of minerals, bioactive compounds and showed better antioxidant property. Therefore, Syzygium cumini seed can be a potential source of natural antioxidant in medicine and food production.

Mineralogical Composition and Bioactive Molecules in the Pulp and Seed of Patauá (Oenocarpus bataua Mart.): A Palm from the Amazon

The Patauá (Oenocarpus bataua Mart.) also known as Bataua or Patuá, is a palm native from the Amazon, consumed among the local populations as well as the wine obtained from its pulp with high energy value. It is a monocle palm tree reaching between 4-26 meters tall, distributed in the Amazon rainforest both in the wet forest of floodplains. The objective of this work was to study the proximal, mineralogical composition, as well as the total phenolic compounds and antioxidant activity of pulp and seed of Patauá. As for mineral composition, the high concentrations of sodium for the seed (84.21 mg 100 g-1) and pulp (71.21 mg 100 g-1), as well as magnesium values of 48.31 mg 100 g-1 for the seed and 41.23 mg 100 g-1 for the pulp. Among the micronutrients, the high concentration of iron in the pulp is 1.84 mg 100 g-1 for the pulps, and the manganese was 1.10 mg 100 g-1 for the seeds. The total phenolic compounds found in the seeds were relatively higher than for the pulps with values of 356.12 ± 0.12 mg GAEq g-1 and 321.03 ± 0.43 mg GAEq g-1, as well as the more significant antioxidant activity for the seeds than for the pulp. Carotenoids concentration in the seeds found of 2.52 ± 0.04 mg mL-1 and vitamin C concentrations were also quantified in trace concentrations, presenting the fruits of Patauá high biotechnological interest in the food and cosmetic industry.

Variation in minerals, polyphenolics and antioxidant activity of pulp, seed and almond of different Ziziphus species grown in Morocco

Abstract Vegetables from the genus Ziziphus (Rhamnaceae) have been identified as one of the main dietary antioxidants, especially phenolic compounds. However, their chemical composition varies according to the specie or the part of the plant. Mineral composition (potassium, magnesium, calcium, sodium, iron, and zinc), polyphenolic content (Flavan-3-ol, Proanthocyanidin, total polyphenol content) and antioxidant capacity (DPPH, ABTS, and ORAC Assay) in four different matrices (whole fruit, pulp, seed and almond) of Ziziphus (Z. Lotus and Z. Spina Christi) commonly grown in Morocco were investigated. As a result, minerals, polyphenolic contents and antioxidant capacity fluctuated significantly (p ≤ 0.05) between different matrices and Ziziphus species analyzed. Seeds displayed a significantly higher mineral level, polyphenolic content and antioxidant capacity in comparison to whole fruit, pulp and almond. Among species, Z. spina-christi have a significantly higher content of mineral, phenolics and antioxidant activity. These results suggest that the mineral level, polyphenolic content and antioxidant capacity vary considerably depending on the Ziziphus species and part of the plant analyzed. Furthermore, Z. spina-christi seeds presented properties desirable for use in functional food.

Phenolic acids contribution to antioxidant activities and comparative assessment of phenolic content in mango pulp and peel

A high performance thin layer chromatography (HPTLC) method to analyse antioxidant activity as free-radical scavenging activity and metal-chelation in selected mango varieties was developed and validated. The contributions of common phenolic acids to antioxidant activity in pulp and peel samples was assessed. Polyphenolic content in pulp and peel was correlated with chlorogenic and gallic acid concentration (R>0.90). Free radical scavenging activity in the peel was related to gallic and chlorogenic acids (R=0.83), but wasn't dependent on caffeic acid concentration. Free radical scavenging activity in the pulp could not be predicted from the gallic or chlorogenic acid (R=0.25 and 0.45) indicating that pulp contains other, more powerful free radical scavengers.

Characterization of phenolics, amino acids, fatty acids and antioxidant activity in pulp and seeds of high altitude Himalayan crab apple fruits (Malus baccata).

Phytochemicals in fruits and vegetables have achieved immense significance owing to the increasing evidence which signifying their activity for antioxidant and prevention of chronic diseases. The amount of phloretin (88.39µgmg-1) and phloridzin (83.03µgmg-1) were found to be higher among other phenolics determined using UPLC. DPPH, ABTS+, metal chelating and ·OH radical assays were used to evaluate antioxidant activity. Malus baccata pulp portion showed higher antioxidant activity than seed portion. HPLC analysis for free amino acids showed that serine (9.06µgmg-1), alanine (8.03µgmg-1), tyrosine (10.33µgmg-1), and cysteine (76.86µgmg-1) were only detected in pulp portion while seed comprised of histidine (3.96µgmg-1) only. Seed portion was also determined for their fatty acid composition including palmitic acid (0.89%), ethyl palmitate (0.56%), methyl petroselinate (0.90%) and linolein (3.93%) using GC-MS analysis. HPAEC technique detected fructose and sucrose in a fair amount of 21 and 17.3mgg-1 in pulp, while 9.4 and 4.24mgg-1 in seed portion, respectively. The present study suggested that M. baccata fruit is a rich source of phenolic and other chemical components which can be used in food products and nutraceutical formulations.

Effect of pasteurisation and freezing method on bioactive compounds and antioxidant activity of strawberry pulp

This study evaluated the stability of strawberry pulp subjected to three factors, pasteurisation (pasteurised and unpasteurised), freezing method (static air and forced air) and storage time (0, 2, 4 and 6 months). Pasteurisation favoured vitamin C retention during storage but enhanced the total loss of phenolics without affecting anthocyanin levels. Freezing by forced air was more effective in retaining phenolics during the first 4 months of storage, although the freezing method did not affect the anthocyanin levels. Processing and storage reduced the levels of individual phenolics. Freezing by forced air was more effective than static air in retaining antioxidant activity of the pulp. Polyphenol oxidase and peroxidase enzyme levels were relatively stable and independent of pasteurisation, freezing and storage time. Even after 6 months of frozen storage, strawberry pulp is a significant source of nutrients and bioactive compounds and retains high antioxidant capacity independent of pasteurisation and freezing method.

Phenolic Profile and Antioxidant Activity of Pulp and Peel from Peach and Nectarine Fruits

Peach (Prunus persica L.) is a fruit of high nutritional and economic value. Carbohydrates, dietary fibers, minerals and organic acids are among the major constituents of peach fruit, which contribute to the nutritional quality of both fresh fruits and juice. Polyphenolic compounds found in peach may play an important role in physiological functions related to human health. Different polyphenolics may have varied biological activities including antioxidant activity. In this study antioxidant characteristics between peel and pulp of different peach cultivars (‘Radmilovčanka’, ‘June Gold’, ‘Blake’, ‘Hale’, ‘Vesna’, ‘Adria’) and one of nectarine (‘Fantasia’) were investigated. The peel and pulp extracts showed a huge amount of total phenolics (TP), total flavonoids (TF), total hydroxycinnamates (TH) and total flavonols (TFL), ranging from 42.7-211.4, 11.1-128.5 mg GAE/100 g fresh weight (f.w.) (TP), 21.9 -94.9, 5.0-58.9 mg CE/100 g f.w. (TF), 28.4-389.2, 8.5-165.8 mg kg-1 f.w. (TH) and 17.3-54 mg kg-1 f.w. (TFL). High contents of phenolic compounds were significantly correlated with high antioxidant capacities. Peach pulp and peel differ significantly in their phenolic profiles: the pulp contains mainly chlorogenic, neochlorogenic and p-coumaric acids, whereas the peel possesses chlorogenic, neochlorogenic and p-coumaric acids together with several flavonol glycosides in huge amounts. Our results indicate that cultivar and extraction solvent play important roles in phenolic compositions and antioxidant properties of peach and nectarine extracts, which was shown using statistical analysis (ANOVA). There are high correlations between extracted phenolic compounds and peach and nectarine cultivars, and used solvent and part of the fruit (peel and pulp).

Phenolic Profile and Antioxidant Activity of Pulp and Peel from Peach and Nectarine Fruits

Peach (Prunus persica L.) is a fruit of high nutritional and economic value. Carbohydrates, dietary fibers, minerals and organic acids are among the major constituents of peach fruit, which contribute to the nutritional quality of both fresh fruits and juice. Polyphenolic compounds found in peach may play an important role in physiological functions related to human health. Different polyphenolics may have varied biological activities including antioxidant activity. In this study antioxidant characteristics between peel and pulp of different peach cultivars (‘Radmilovčanka’, ‘June Gold’, ‘Blake’, ‘Hale’, ‘Vesna’, ‘Adria’) and one of nectarine (‘Fantasia’) were investigated. The peel and pulp extracts showed a huge amount of total phenolics (TP), total flavonoids (TF), total hydroxycinnamates (TH) and total flavonols (TFL), ranging from 42.7-211.4, 11.1-128.5 mg GAE/100 g fresh weight (f.w.) (TP), 21.9 -94.9, 5.0-58.9 mg CE/100 g f.w. (TF), 28.4-389.2, 8.5-165.8 mg kg-1 f.w. (TH) and 17.3-54 mg kg-1 f.w. (TFL). High contents of phenolic compounds were significantly correlated with high antioxidant capacities. Peach pulp and peel differ significantly in their phenolic profiles: the pulp contains mainly chlorogenic, neochlorogenic and p-coumaric acids, whereas the peel possesses chlorogenic, neochlorogenic and p-coumaric acids together with several flavonol glycosides in huge amounts. Our results indicate that cultivar and extraction solvent play important roles in phenolic compositions and antioxidant properties of peach and nectarine extracts, which was shown using statistical analysis (ANOVA). There are high correlations between extracted phenolic compounds and peach and nectarine cultivars, and used solvent and part of the fruit (peel and pulp).

Evaluation of antioxidant activity and polyphenolic contents of two South Algerian eggplants cultivars

Polyphenolic compounds and antioxidant activity in eggplants ( Solanum melongena L.) seem to depend on cultivar, maturity stage, environmental conditions and the part of the fruit. In this work, the polyphenolic content and antioxidant activity of pulp, whole fruit and peel from fresh dark-purple and white eggplant varieties cultivated in Southern Algeria were measured. Total phenolic content were determined by Folin-Ciocalteu test, and antioxidant activity measured using cyclic voltammetry. Correlations between antioxidant activity and total phenolic content were also examined. Within each cultivar, the total phenolic content and antioxidant activity were highest in the peels, followed by the whole fruit and the pulp. In the peel, whole fruit and pulp the dark-purple eggplant had the highest total phenolic contents and the highest total antioxidant activities. Keywords: Solanum melongena L.; antioxidant capacity; total phenolic content; cyclic voltammetry

Antioxidant Activity in Pulp and Peel of Three Mango Varieties

The aim of the present study was to estimate the content of total polyphenols and flavonoids and to investigate in-vitro antioxidant potential of methanolic extracts of peel and pulp in three Indian mango varieties. Antioxidant activity was assessed using [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] ABTS+ assay, 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, ferric-reducing ability of plasma (FRAP) assay, and phosphomolybdate assay for Total Antioxidant Capacity (TAC). Total phenolic and flavonoid content was also determined, and expressed in gallic acid equivalent (GAE) and quercetin equivalent (QE), respectively. Results of this study indicated that methanolic extracts of mango peel had significantly higher antioxidant activity compared to that of pulp (29.69 and 3.12), irrespective of the method or variety used. Free radical scavenging and antioxidant activity may be attributed to presence of phenolic (24.61mg GAE/g DM in the peel and 2.01mg GAE/g DM in the pulp) and flavonoid compounds (24.95mg QE/g DM in the peel and 16.15mg E/g DM in the pulp). Antioxidant activity determined by ABTS, DPPH and FRAP assays in mango peel was significantly higher than in the mango pulp (24.95 1.96mg TE /g DM, 23.68 versus 4.60mg BHA/g DM and 40.52 versus 2.781mg TE/g DM), respectively. Results for scavenging activity against DPPH were 96.18% for the peel and 23.86% for the pulp, while, free radical scavenging activity results using ABTS+ assay were 99.62% in the peel and 13.46% in the pulp. Our study justifies research in processing of mango peel into useful, functional food ingredients (powders or extracts).

Antioxidant Activity and Phenolic Content of Seed, Skin and Pulp Parts of 22 Grape (Vitis vinifera L.) Cultivars (4 Common and 18 Registered or Candidate for Registration)

Total phenolic contents and antioxidant activities of pulp, seed and skin of 22 grape varieties (7 white and 15 red) grown in the Marmara region of Turkey were determined (common, registered or candidate cultivars). The total phenolic contents of grape pulp, seed and skin parts ranged from 9.26 to 62.29, from 162.29 to 326.18 and from 96.61 to 167.42 mg gallic acid equivalents/100 g fresh weight, respectively. Seasonal changes were noticeable in the total phenolic contents and antioxidant activities of different grape parts. The antioxidant activity of grape seeds of registered or candidate cultivars was the highest, followed by skins and pulps. The antioxidant activities of grape skins were higher in red varieties than in white varieties. The results indicated that registered and candidate red or white grape cultivars may have high amounts of phenolics and possess a superior antioxidant activity in comparison to popular cultivars, such as Bilecik Irikarasi, Hamburg Misketi, Alfons and Isabella. Practical Applications Grapes are an important commercial commodity because they can be consumed fresh or processed into many food products such as juice, jams, raisins and wine. Chemical properties of grapevines are one of the most important factors that determine the industrial use of grape berries. A great deal of diverse phenolic compounds is located in the skins, pulp and seeds of grapes. The antioxidant activity of different parts of grapes has been studied in popular and hybrid varieties; however, studies should also be continued on hybrid varieties to determine better grape berries with high antioxidant activity and phenolic content. Berries with better quality in terms of antioxidant activity may present great potential for the food industry as well as health-conscious consumers.