Antioxidant Activities Of Flower Extracts Research Articles

Detection of Antioxidant and Antimicrobial Components from Crude Extracts of Hibiscus Arnottianus A. Gray flowers using HPTLC-Bioautographic Technique

Abstract: Hibiscus arnottianus belonging to family, malvaceae, is a herb used in traditional medicine for the treatment of inflammation and renal diseases. Methanolic extract of Hibiscus arnottianus flower was fractionated using different solvents of increasing polarity like petroleum ether, ethylacetate, methanol and water. The preliminary phytochemical screening showed the presence of carbohydrates, steroids, flavonoids, tannin. Methanolic extract of hibiscus arnottianus flower was screened for their antimicrobial and antioxidant activity. The test microorganisms includ Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa. It was concluded that Hibiscus arnottianus flowers extract was well effective against all these microorganisms. HPTLC-DPPH assays were used to identify the antioxidant activity of flower extract. This activity was estimated by HPTLC bioautographic method by using the mobile phase Chloroform:Ethyl Acetate:Methanol:Water (2.8:5.2:1.6:0.4 v/v/v/v). There was a successful chromatogram developed and detection of a band in the sample was observed. Later, the band showing antioxidant characteristic property was identified by DPPH Assay.

SUGAR AND PROTEIN CONTENT, HYDROGEN PEROXIDE RADICAL SCAVENGING AND TOTAL ANTIOXIDANT CAPACITY OF Polianthes tuberosa L FLOWERS GROWN IN ALGERIA

Tuberose flowers (Polianthes tuberosa L.) belong to the Amaryllidaceae family and they are widely enhanced through their absolute and essential oils, especially in the perfume industry. However, the sugar and proteins of tuberose are still unknown. For this reason, this work aimed to quantify them, particularly in flowers, besides to the evaluation of their hydrogen peroxide radical scavenging and total antioxidant capacity. The obtained results revealed 79.51±0.08 mg glucose equivalent/g extract of sugar and 0.097±0.001 mg bovine serum albumin equivalent/g extract of proteins. These metabolites were quantified using phenol-sulfuric acid and Coomassie Blue methods, respectively. Furthermore, the antioxidant activity of flower extract was investigated using total antioxidant capacity (TAC) and hydrogen peroxide radical scavenging. The TAC assay indicated the inhibitory concentration of 50% of free radicals (IC50) was 0,09±0,01 mg/mL of flower extract, however, the scavenging ability against H2O2 showed the IC50 of 7,09±0,15 mg/mL of tuberose flower extract. This work contributes to the knowledge of tuberose flowers content in proteins and sugar, in addition to the total antioxidant and the H2O2 scavenging capacities.

Comparison of efficacy of nanoparticles synthesized from leaves and flowers of Russelia equisitiformis

The present study was focused on synthesis of silver nanoparticles using Russelia equisitiformis plant (RelAgNps) and flower extracts (RefAgNps). Thus, the nanoparticles synthesis was confirmed using UV spectrometry and the FT-IR results showed the presence of similar compounds in the plant extract and FESEM-EDAX reveals the size, shape, and elemental composition of synthesized nanoparticles. The antioxidant activity of the RefAgNps from flower extract and RelAgNps from leaf extract was observed at the concentration of 25 μg/mL but the percentage of antioxidant activity of flower extract was comparatively higher than the leaf extract. This plant is also known to have antidiabetic activity and thus leaf extract and flower extract mediated nanoparticles is also known to have alpha amylase inhibition activity at the concentration of 12.5 and 100 μg/mL, respectively. Molecular docking of the plant compounds10-O-cinnamoyl sinuatol and rehmaglutin B were docked with alpha amylase enzyme proves the same with the binding energy of −9.1 kcal/mol. Thus, to the best of our knowledge this is the first-time nanoparticles have been synthesized using R. equisitiformis flower and leaf extract and observed to have potent antioxidant and antidiabetic activity.

Celosia cristata Linn. flowers as a new source of nutraceuticals- A study on nutritional composition, chemical characterization and in-vitro antioxidant capacity

This study was undertaken to investigate the nutritional value, chemical characterization and in-vitro antioxidant activity of Celosia cristata Linn. inflorescences, a culturally significant plant of Kashmir valley, India. The results revealed that the flower contained variety of vitamins (A, B-complex, C and E) with Vitamin E (tocopherol) showing the highest concentration. Among minerals, potassium was found to be present in significant amounts, the amino acid and fatty acid profile of the flower was also found to be satisfactory. The antioxidant activity of flower extract was evaluated by various in-vitro analytical methods: DPPH free radical scavenging activity, lipid peroxidation, reducing power, and metal chelating ability. Therefore, the present research brings into focus, the nutritional and antioxidant potential of C. cristata flower and its extract.

Toxicity and antidiabetic activity of ethanolic extract of Sphagneticola trilobata (L.) Pruski flower in rats

Ethnopharmacological relevancePlants are the source of medication for preventive, curative, protective or promotive purposes. Medicinal plants are an important source for generating of novel phytomedicine. They provide profound therapeutic benefits, more affordable treatments, effectiveness, less side effects and relatively low cost or less expensive and globally competitive. Using plant derived medicine is also relatively safe compared to synthetic medicines. Many plants have proved to successfully aid in the treatment of ailments including Sphagneticola triolobata (L.) Pruski. Aim of studyThis study was therefore, designed to investigate acute and subacute toxicities, antidiabetic activity and also antioxidant activity of flower extract from S. triolobata (L.) Pruski. MethodsThis research investigates the toxicity and antidiabetic activity of Sphagnelicola trilobata (L.) Pruski flower ethanolic extract in rats. Acute toxicity was determined by a single oral administration of S. trilobata extract of 1500, 2000, and 2500 mg/kg body weight; and subacute toxicity by oral administration every two days for 14 days. Signs of toxicity and mortality were observed during 24 h and for 14 days. Hematological values and blood chemistry were also characterized. The antidiabetic activity was examined by orally administering S. trilobata extract of 250 mg/kg body weight to streptozotocin-induced diabetic rats on a daily basis for eight weeks; and the body weight, blood glucose, serum insulin, and lipid profiles were determined. The antioxidant activity of the extract was assessed by 1, 1-diphenyl-2-picryl-hydrazyl free radical scavenging assay. Results and conclusionThe results demonstrated a median lethal dose (LD50) greater than 2500 mg/kg since there was no sign of toxicity and mortality in acute and subacute toxicity testing. The high LD50 indicated that S. trilobata flower ethanolic extract is safe for treatment of diabetes. There was no significant change in the body weight, hematological values, and blood chemistry of treated rats, compared with the control group. The diabetes-induced rats showed a significant reduction in blood glucose and triglyceride (p < 0.05). Meanwhile, the antioxidant activity of S. trilobata extract was lower than that of standard ascorbic acid.

Evaluation of the phytochemical constituents total phenol, total flavonoid and anti-oxidant activity of Delonix elata flower extract

The objective of the present study was to evaluate the phytochemical constituents, total phenol, total flavonoid, anti-oxidant activity of flower extract of Delonix elata. Plants are widely used in pharmaceutical and food industries due to their biological importance. Among the plant parts, leaves, stem, roots and bark are widely studied for their biological properties. However, flowers are almost neglected and are not much probed for their importance. The present study was carried out to identify the phytochemicals and evaluate antioxidant activity of flowers of D. elata. The antioxidant activity was determined by the method of DPPH radical scavenging assay. The flower extract contain saponin, alkaloid, terpenoids, flavonoids, steroids, phenols, cardioglycosides, quinines, coumarins and tannins. Thus, clearly indicate that the flower extract of D. elata shows significant antioxidant activity which in turn greatly contribute in reducing the risk of many diseases including heart disease, cancer cell formation and cell physiological abnormalities.

Optimization of Ultrasound-Assisted Extraction of Natural Antioxidants from the Flower of Jatropha integerrima by Response Surface Methodology.

An ultrasound-assisted extraction (UAE) method was developed for the efficient extraction of natural antioxidants from the flowers of Jatropha integerrima. Four independent variables, including ethanol concentration, solvent/material ratio, ultrasound irradiation time and temperature were studied by single factor experiments. Then, the central composite rotatable design and response surface methodology were employed to investigate the effect of three key parameters (ethanol concentration, solvent/material ratio, and ultrasound irradiation time) on the antioxidant activities of the flower extracts. The optimal extraction conditions were an ethanol concentration of 59.6%, solvent/material ratio of 50:1, ultrasound irradiation time of 7 min, and ultrasound irradiation temperature of 40 °C. Under these conditions, the optimized experimental value was 1103.38 ± 16.11 µmol Trolox/g dry weight (DW), which was in accordance with the predicted value (1105.49 µmol Trolox/g DW). Furthermore, the antioxidant activities of flower extracts obtained by UAE were compared with those produced by the traditional maceration and Soxhlet extraction methods, and UAE resulted in higher antioxidant activities after a shorter time at a lower temperature. The results obtained are helpful for the full utilization of Jatropha integerrima, and also indicate that ultrasound-assisted extraction is an efficient method for the extraction of natural antioxidants from plant materials.

Antioxidant and antidiabetic activities of flower extract from Butea monosperma (Lam.) Taub.

Flowers of Butea monosperma (Lam.) Taub. have been widely used in treatment of various diseases and different purposes such as antifertility, antidiarrhoea, antifungal, antiinflammatory, antimicrobial, anticancer, antihelmintic, and antidiabetic properties. However, few studies on antioxidant and antidiabetic activities of flower extract from this plant have been established. The present study was therefore, carried out to investigate antioxidant and antidiabetic activities of flower extract from Butea monosperma. The plant flowers were shade dried, powdered and extracted with 80% ethanol. Antioxidant activity of flower extract from Butea monosperma (BMFE) was investigated in vitro model using 2,2 diphenyl-1- picrylhydrazyl (DPPH) radical scavenging assay. An antidiabetic activity of BMFE was conducted in streptozotocin-induced diabetic rats by oral administration of BMFE at a dose of 250 mg/kg once daily to the rats for 8 weeks. Body weight and fasting blood glucose level were examined weekly. Additionally, after eight weeks of treatment, serum insulin, lipid profile and blood chemistry of the rats were investigated. The results revealed that BMFE showed low potent free radical scavenging with IC50 at 231.64+0.99 μg/ml compared to L-ascorbic acid (1.48+0.07μg/ml). BMFE reduced (p<0.05) blood glucose level, and increased (p<0.05) serum insulin, blood urea nitrogen (BUN), creatinine, alkaline phosphatase (ALP) and total protein (TP). Nevertheless, BMFE slightly increased triglyceride (TG) and total cholesterol (TC) in the diabetic rats. These findings indicate that the flower extract from Butea monosperma possesses antioxidant activity and antidiabetic activity which lowering blood glucose level by increasing insulin secretion. Long term utilization of the extract should be considered as it affects hepatic and renal function.

Antioxidant activity of Spathodea campanulata (Bignoneaceae) extracts

Spathodea campanulata is used in traditional medicine in Africa as diuretic and anti-inflammatory. Although few studies have reported the mechanism of antioxidant action, this study evidenced the antioxidant activity of S. campanulata bark and flower extracts and their possible mechanism of action. Ethanol extracts of S. campanulata bark and flowers showed antioxidant activity on lipid peroxidation of liver microsome induced by Fe3+-ascorbic acid. Bark extract was 5 times more efficient than flower extract. The antioxidant activity of flower extract, previously complexed with increasing concentrations of Fe3+ (20 - 100 μM) which resulted in antioxidant activity loss, was shown to be related to iron complex formation. In contrast, the antioxidant activity of bark extract was not inhibited by the previous incubation with Fe3+, although complexation was demonstrated by spectral analysis of the solution. These results suggest an antioxidant mechanism other than Fe3+ complex formation. Therefore, the antioxidant mechanisms of S. campanulata flower and bark extracts are distinct from each other, reflecting the extract heterogeneous composition and the mechanism of action.