Antimicrobial Efficacy Of Sodium Hypochlorite Research Articles

Influence of Organic Matter from Native Fish on the Antimicrobial Efficacy of Sodium Hypochlorite (NaClO) in Reducing Salmonella spp. Population

Salmonella spp. is not part of the fish microbiota, being introduced through contaminated water or improper handling. In slaughterhouses, five parts per million (ppm) of free chlorine is recommended in the washing step to eliminate this pathogen. However, Salmonella spp. is still detected in fish processing plants due to persistent contamination as a result of chlorine–organic matter interactions that reduce chlorine effectiveness. Therefore, this study aimed to test whether organic matter contained in culture media and fish washes interferes with the efficacy of chlorine at 2 and 5 ppm, and what is the time of action required to inactivate ATCC and wild strains of S. typhimurium. For this, the elimination or survival of these strains was investigated when exposed to chlorinated solution for different durations (0, 5, 30, 60, 90 and 120 min) in culture medium, 0.85% saline solution and fish wash (in natura and sterilized). The results showed that the use of 5 ppm of free chlorine is efficient, even when the chlorine remains in contact with the organic matter (washed from the fish), as it reduced the bacterial population by ~5 log log10 CFU/mL of Salmonella spp., showing that this reduction was due to interactions, as long as the chlorine action time was 30 min.

Antimicrobial efficacy of sodium hypochlorite and hyper-pure chlorine dioxide in the depth of dentin tubules in vitro

ObjectivesThe study aimed to compare the antibacterial effect of a novel disinfectant, hyper-pure chlorine dioxide (hClO2) to sodium hypochlorite (NaOCl) in various depths of dentin tubules.Materials and methodsThe distal root of the extracted lower molars was infected artificially with Enterococcus faecalis. The control group was rinsed with saline, and the test groups were irrigated with either 5% NaOCl or 0.12% hClO2. The longitudinally split teeth were stained by viability stain. The coronal third of the root was scanned with a confocal laser scanning microscope. The fluorescent intensities were measured, and the percentage of dead bacteria was calculated at depths up to 950 μm along the dentin tubules. The effect of penetration depth, irrigants, and their interaction on antimicrobial efficacy was determined by the linear mixed model.ResultsThe percentage of dead bacteria was higher both in the NaOCl (45.1 ± 2.3%, p < 0.01) and in the hClO2 (44.6 ± 3.8%, p < 0.01) irrigant groups compared to saline (23 ± 4.5%); however, there was no difference between them. The percentage of killed bacteria was not correlated with the depths in any group (p = 0.633).ConclusionsOur results suggest that the functional penetration depth of NaOCl is at least 2–3 times more than published to date. There is no difference in disinfection effectiveness along the dentin tubules between NaOCl and hClO2 until at least the measured 950 μm. However, both were only able to eradicate the intratubular bacteria partially.Clinical relevanceHyper-pure ClO2 could be used as an alternative or final adjuvant irrigant in endodontic treatment.

Antimicrobial Efficacy of Sodium Hypochlorite and Er, Cr: YSGG Laser against E Faecalis Biofilm - A Systematic Review

Background: The complexity of the root canal system makes it difficult to completely disinfect is the leading cause for endodontic retreatment. Sodium hypochlorite (NaOCl) is the traditionally used irrigation solution and has improved its efficacy with various adjuncts. The use of lasers in disinfecting the canal system has come into prominence in recent years. Aim: To assess the efficacy of sodium hypochlorite and Er, Cr: YSGG laser against E faecalis biofilm. Methodology: A systematic search was conducted using a search strategy in PubMed and Google scholar for studies published between 1st January 2011 and 21st March 2022. Hand searching of relevant articles in Institutional library was also performed. Comparative in vitro studies in English language or articles in other languages which had a possibility of translation into English comparing sodium hypochlorite and Er, Cr: YSGG laser for irrigation against E faecalis biofilm were included in the review whereas, studies comparing efficacy against biofilm other than E faecalis, comparison with other laser activated intracanal irrigation, data reported through letter to editors, short communications and conference proceedings were excluded from the review. Results: A preliminary search yielded a total of 67 studies of which seven articles giving 11 estimates were included for qualitative synthesis. Data on colony forming units for both the groups (sodium hypochlorite and Er, Cr: YSGG laser) was extracted. Overall, four studies presented significant difference in the effectiveness of sodium hypochlorite and Er, Cr: YSGG laser whereas three studies presented with no difference among the two groups. Conclusion: Irrigation of root canal by sodium hypochlorite and Er, Cr: YSGG laser activation both prove to be effective in reducing the E faecalis colony forming units. However, it is difficult to provide a conclusive statement on the superiority of Er, Cr: YSGG laser activated irrigation over sodium hypochlorite solution as half of the studies gave contrasting results. Future studies with larger sample size on complex anatomical root structure are recommended.

Analysis of antimicrobial efficacy of sodium hypochlorite and ozonated water against biofilm in oval canals.

This in vitro study compared the antimicrobial efficacy of 2.5% sodium hypochlorite (NaOCl) and 8 µg/mL ozonated water agitated by passive ultrasonic irrigation (PUI) or PUI combined with EndoActivator (EA) against mature multispecies biofilm. One hundred and five oval-shaped mandibular premolars were instrumented, sterilized, and inoculated with Enterococcus faecalis, Candida albicans, and Staphylococcus aureus, divided into: control group - saline; O3 group - ozonated water; O3 PUI group - ozonated water with PUI agitation; O3 PUI+EA group - ozonated water with PUI+EA agitation; NaOCl group - NaOCl; NaOCl PUI group - NaOCl with PUI agitation; and NaOCl PUI+EA group - NaOCl with PUI+EA agitation. Microbiological samples were collected before (S1) and after (S2) the disinfection procedures and the data were statistically analyzed using the Kruskal-Wallis test. In the culture method, there was significant disinfection in the O3 PUI+EA, NaOCl, NaOCl PUI, and NaOCl PUI+EA groups (p˂0.05). The combination of NaOCl with PUI+EA reduced microbial counts to zero (p˂0.05). In the qPCR method, there was a significant reduction in the total count of viable microorganisms in the O3 PUI, O3 PUI+EA, NaOCl, NaOCl PUI, and NaOCl PUI+EA groups (p˂0.05). It can be concluded that 2.5% NaOCl with and without agitation, as well as 8 µg/mL ozonated water with its action enhanced by the agitation techniques, were effective in root canal disinfection, and their antimicrobial efficacy is related to the microorganisms present in the biofilm.

Comparative Evaluation of Antimicrobial Efficacy of Sodium Hypochlorite, Triphala, Eucalyptus, and Carvacrol against Enterococcus faecalis: An In Vitro study.

This study aimed to evaluate the antimicrobial effect of sodium hypochlorite (NaOCl), Triphala, Eucalyptus, and carvacrol on Enterococcus faecalis as the most common microorganism isolated from infected root canals. Seventy-five mandibular premolar teeth were randomly distributed into five study groups: 5.25% NaOCl, 10% Triphala, 1.25% Eucalyptus, 0.6% carvacrol, and negative control (saline) group. Samples were taken using paper points from the canal spaces and using Gates-Glidden (GG) drills from dentinal tubules; after sample culturing, the colony forming unit (CFU) were counted, which was analyzed by the Wilcoxon signed-rank test. All irrigants have shown a reduction of microorganisms in the root canal space. After the use of NaOCl and Eucalyptus, the bacterial count was significantly reduced in the canal, as well as dentin sampling, as compared to Triphala and carvacrol. The antimicrobial efficiency of all irrigants against E. faecalis revealed a significant difference (p < 0.05). All irrigants exerted significant antimicrobial activity against E. faecalis. Around 1.25% of Eucalyptus was the most effective irrigant than 5.25% of NaOCl, Triphala, and carvacrol. Panchal VV, Dahake PT, Kale YJ, et al. Comparative Evaluation of Antimicrobial Efficacy of Sodium Hypochlorite, Triphala, Eucalyptus, and Carvacrol against Enterococcus faecalis: An In Vitro study. Int J Clin Pediatr Dent 2022;15(5):514-519.

Antimicrobial efficacy of sodium hypochlorite, saline, and Er, Cr:YSGG laser-assisted disinfection in root canal treatment of primary molars: An in vivo study.

The aim of the study was to compare the antimicrobial efficacy of sodium hypochlorite (NaOCl), Saline, and Er, Cr:YSGG laser-assisted disinfection through microbiological analysis in root canal treatment of primary molars. Forty-five primary teeth were selected for this study as per the inclusion and exclusion criteria in the age group of 4-8 years and then randomly allocated to Three Groups A, B, and C. In Group A, during root canal treatment, irrigation was done using NaOCl, in Group B, laser-assisted disinfection was done using Er, Cr:YSGG laser, and in Group C, saline was used as an irrigating agent. This was followed by obtaining pre- and postirrigation samples from the canals for microbiological investigation in all the groups. Data analysis was performed using Statistical Package for the Social Science-21 (SPSS-21). NaOCl was more effective against aerobic bacteria while Er, Cr:YSGG laser was more effective against anaerobic bacteria when used for disinfection in root canal treatment. There was a significant difference among the three groups (P > 0.05). The study showed that NaOCl and Er, Cr:YSGG laser have antimicrobial effects when used for disinfection in root canal treatment of primary teeth. Moreover, the study also shows that Er, Cr:YSGG laser may be used as a valuable tool for laser-assisted disinfection in root canal treatment of primary teeth.

COMPARITVE STUDY OF SMEAR LAYER REMOVAL AND ANTIBACTERIAL ACTIVITY OF SILVER, CHITOSAN NANOPARTICLES AND SODIUM HYPOCHLORITE AGAINST ENTEROFECALIS WHEN USED AS ROOT CANAL IRRIGANTS- IN VITRO STUDY

Background: As a very resistant microorganism in infected root canals, Enterococcus faecalis (E. faecalis) can stubbornly survive lethal challenges and invade dentinal tubules, making it the most persistent pathogen in root canal treatment. Enterococcus faecalis (E. faecalis) is the most commonly encountered microorganism detected in persistent root canal infections. These bacteria possess certain virulence factors, invade dentinal tubules and resist nutritional deprivation. Proper irrigation is an essential step for success in root canal therapies which is achieved by using excellent endodontic irrigants. Aim: To evaluate the antimicrobial efficacy of Sodium Hypochlorite (NaOCl), Silver Diamine Fluoride (SDF), Chitosan Nanoparticles (CNPs) root canal irrigants against the bacterial strain of Enterococcus Faecalis (E. faecalis) using agar well diffusion method. Materials and Methods: In this in-vitro study, the test materials were manipulated in accordance with the manufacturer’s instructions. The antimicrobial properties of root canal irrigants were evaluated by using agar diffusion method using bacterial strain of Enterococcus faecalis (ATCC 29212). 0.25 mL of each irrigant was placed on to 6.5 mm diameter blotting papers which were placed in 7 mm diameter wells on the Mueller Hinton agar plates. Later, E. faecalis strains were inoculated with sterile cotton swab on to the agar plates. Results: The p-value Sodium Hypochlorite showed the greatest zone of inhibition followed by SDF and Chitosan Nanoparticles respectively (p Sodium Hypochlorite showed the greatest zone of inhibition followed by SDF, Bioactive Glass Nanoparticles and Chitosan Nanoparticles respectively. Conclusion: Sodium Hypochlorite was the most effective root canal irrigant followed by SDF ,whereas Chitosan Nanoparticles was the least efficacious compared to the rest against Enterococcus Faecalis.

Antimicrobial efficacy of sodium hypochlorite, neem, Tulsi, and Aloe vera as a root canal irrigants against E. faecali

Root canal irrigation helps in eliminating the microorganisms present in the canal system. The present in vitro study was done to evaluate the efficacy root canal irrigants; sodium hypochlorite, neem, Tulsi, and Aloe vera against E. faecali. In present study 5 groups of root canal irrigants (sodium hypochlorite, neem, Tulsi, Aloe vera, and distilled water as controlled group) were tested for efficacy against E. faecali. There was highest inhibition zone observed with sodium hypochlorite group followed by Neem (Azadirachta indica), neem, Tulsi, Aloe vera and least was observed in control group with distilled water. The tested root canal irrigants sodium hypochlorite group followed by Neem (Azadirachta indica), Tulsi, and Aloe vera are effective against E. faecali.

Antimicrobial Efficacy of Calcium and Sodium Hypochlorite at Different Concentrations on a Biofilm of Enterococcus faecalis and Candida albicans: An In Vitro Comparative Study

To compare the antimicrobial efficacy of sodium hypochlorite (NaClO at 2.5% and 5.25%) and calcium hypochlorite [Ca(ClO)2 at 2.5%] on a biofilm of Enterococcus faecalis ATCC 29212™ and Candida albicans ATCC 10231™. We performed an experimental in vitro study. Strains of C. albicans and E. faecalis, which had previously been reactivated were used. Then the colonies to be used were standardized in a turbidity standard to guarantee a quantity of 108 (CFU/mL) using the McFarland scale (0.5). Subsequently, the biofilm formed in brain-heart infusion agar was seeded into 42 sterile disks previously embedded with the experimental substances. Both 2.5% NaClO and Ca(ClO)2 solutions were placed in each Petri dish. They were then incubated at 37°C for 24 hours and the inhibition halos were measured using the Kirby-Bauer technique. The means between the halos corresponding to NaClO and Ca(ClO)2 at 2.5% were 13.38 ± 0.64 mm and 13.42 ± 0.62 mm, respectively. According to the Tukey test, no statistically significant differences were found between the hypochlorite groups evaluated (p = 0.989). Both Ca(ClO)2 and NaClO have a similar antimicrobial efficacy with biofilm based on E. faecalis and C. albicans, with no statistically significant differences between the two. This study demonstrates the effectiveness of Ca(ClO)2 and NaClO as endodontic irrigators to combat the most frequent microorganisms of the root canal.

Comparative Evaluation of Antimicrobial Efficacy of Sodium Hypochlorite, Silver Diamine Fluoride, Chitosan and Bioactive Glass Nanoparticles as Root Canal Irrigants against the Bacterial Strain of Enterococcus faecalis- An In vitro Study

Comparative Evaluation of Antimicrobial Efficacy of Sodium Hypochlorite, Silver Diamine Fluoride, Chitosan and Bioactive Glass Nanoparticles as Root Canal Irrigants against the Bacterial Strain of Enterococcus faecalis- An In vitro Study

Effect of conventional irrigation and photoactivated disinfection on Enterococcus faecalis in root canals: An in vitro study.

Aims:A study was done to evaluate the antimicrobial efficacy of sodium hypochlorite (NaOCl) and photoactivated disinfection (PAD) on Enterococcus faecalis.Settings and Design:Random sampling, in-vitro study.Subjects and Methods:Access opening and biomechanical preparation were performed on fifty freshly extracted mandibular second premolars. The specimens were sterilized; 15 μm of E. faecalis was inoculated into each canal and incubated at 36°C for 24 h. Later, specimens were randomly divided into two groups of fifty each and following procedures was carried out: (i) conventional irrigation with 2.25% NaOCl (ii) PAD using diode laser, and toluidine blue photosensitizer. Samples were collected from each canal using sterile paper points which were deposited in brain heart infusion broth, and microbiological evaluation was carried out.Statistical Analysis Used:Student's t-test was used to find the significant difference in the reduction of colony forming unit (CFU) between the groups.Results:The mean CFUs of the two groups showed statistically significant difference (P = 0.001). Improved antibacterial efficacy was seen with PAD group compared to conventional NAOCL irrigation.Conclusions:NaOCl alone was not effective in eliminating E. faecalis completely from the root canals. PAD compared to conventional irrigation showed the best results in removing E. faecalis from root canals.

Antimicrobial Efficacy and Cytocompatibility of Calcium Hypochlorite Solution as a Root Canal Irrigant: An in Vitro Investigation.

Introduction:The purpose of this study was to compare the antimicrobial efficacy of sodium hypochlorite (SH) and calcium hypochlorite (CH) against Enterococcus faecalis (E. faecalis) using quantitative real-time polymerase chain reaction (qPCR) analysis and also to compare their cytocompatibility on L929 murine fibroblasts using Mossman’s tetrazolium toxicity (MTT) assay. Methods and Materials:A broth micro-dilution susceptibility test was used to determine the minimum inhibitory concentration (MIC) of each irrigant against E. faecalis. Then, the root canals of 50 mature extracted human mandibular premolars were contaminated with E. faecalis and were randomly divided into three groups according to the irrigant used (n=20). Canals were irrigated with SH in group I (n=20) and CH in group II (n=20) at their obtained MIC. In group III (n=10), sterile saline was used. Microbial sampling was performed before and after biomechanical preparation. Quantitative PCR was used to quantify E. faecalis in the root canal samples. For cytocompatibility assessment, L929 murine fibroblasts were exposed to various concentrations of the irrigants. Results:Irrigation with test materials resulted in significant reduction in colony forming units (CFU) in post-instrumentation samples (with the MIC values of SH and CH against E. faecalis being 0.5% and 5%, respectively). However, the reduction in the normal saline group was not significant (P=0.203). In addition, 5% CH was more effective than 0.5% SH (P=0.006) in eliminating E. faecalis. Among the different concentrations of tested irrigants, 0.5% CH and 5% SH showed the least and the most cytotoxicity, respectively (P<0.001). The cytotoxicity of 5% CH and 0.5% SH was similar (P=0.99), and lower than 2.5% SH (P<0.001). Conclusion: CH at an MIC of 5% was effective in eliminating E. faecalis in planktonic state and also its biofilm and exhibited comparable cytocompatibility to that of 0.5% SH.

Evaluation of antimicrobial efficacy of sodium hypochlorite, propolis, octenidine dihydrochloride and chlorhexidine on microorganisms

Evaluation of antimicrobial efficacy of sodium hypochlorite, propolis, octenidine dihydrochloride and chlorhexidine on microorganisms

Antimicrobial efficacy of 4.2% sodium hypochlorite adjusted to pH 12, 7.5, and 6.5 in infected human root canals

The purpose of this study was to determine the antimicrobial efficacy of sodium hypochlorite adjusted to pH 12, 7.5, and 6.5 in human root canals infected by Enterococcus faecalis. One hundred sixty-five human single-rooted teeth were prepared and inoculated with E. faecalis for 48 h. Teeth were divided into 3 experimental groups according to the irrigation pattern used: group 1, 4.2% NaOCl pH 12; group 2, 4.2% NaOCl pH 7.5; and group 3, 4.2% NaOCl pH 6.5. Samples from the root canals were collected, and bacterial growth was analyzed by turbidity of the culture medium. None of the irrigating solutions used in this study demonstrated 100% effectiveness against E. faecalis. The antibacterial effectiveness of 4.2% NaOCl at pH 6.5 was significantly increased (P = .03) compared with 4.2% NaOCl at pH 12 (chi-squared test: P < .05). Bactericidal activity of NaOCl solution is enhanced by weak acidification of 4.2% NaOCl solution at pH 6.5.

Antimicrorobial evaluation of sodium hypochlorite and ozonated water on E. faecalis biofilm

This study aim to evaluate antimicrobial efficacy of sodium hypochlorite (NaOCl) and ozonated water against Enterococci faecalis biofilm. The bacterial biofilm was exposed to 2.62%, 1.31% NaOCl and 0.1 ppm ozonated water over a range of time periods. The presence of viable cells was determined by enumeration of colony forming units (CFU). All experiments were repeated four times (n=4). The effectiveness of the agents was compared using nonparametric Kruskal- Wallis test. The result revealed that 2.62% of NaOCl can completely kill E. faecalis biofilm in 15 minutes whereas 1.31 % NaOCl required a longer time to produce such effect. 0.1 ppm ozonated, however, did not exhibit any antimicrobial effect within the period of time tested. From this study, it can be concluded that 0.1 ppm ozonated water was not comparable with 2.62% and 1.31% NaOCl in antimicrobial efficacy against E. faecalis biofilm.

Comparative Evaluation of Antimicrobial Efficacy of Sodium Hypochlorite, MTAD, and Tetraclean Against Enterococcus faecalis Biofilm

The aim of this study was to compare the antimicrobial efficacy of 5.25% NaOCl, BioPure MTAD (Dentsply Tulsa Dental, Johnson City, TN), and Tetraclean (Ogna Laboratori Farmaceutici, Milano, Italy) against Enterococcus faecalis biofilm generated on cellulose nitrate membrane filters. After incubation, the membrane filters were transferred into tubes containing 5 mL of the selected antimicrobial solution test agent or NaCl 0.9% (positive control) and incubated for 5, 30, and 60 minutes at 20 degrees C. After each period of time, the test agents were vortexed for 60 seconds to resuspend the microorganisms. Ten-fold serial dilutions were generated in reduced transport fluid. Each dilution was plated onto a brain heart infusion plates. The plates were then incubated for 48 hours in an aerobic atmosphere at 37 degrees C and colony-forming units per membrane was calculated. Statistical analysis showed that only 5.25% NaOCl can disgregate and remove the biofilm at every time; however, treatment with Tetraclean caused a high degree of biofilm disgregation in every considered time intervals as compared with MTAD (T5 p < 0.05, T30 p < 0.01, and T60 p < 0.001).