Propolis is a natural bee product that has been used as a therapeutic agent for centuries. Propolis extracts are natural resources that attract the attention of scientists looking for new components due to the insufficiency of existing drugs. In the current study, the antiviral and antimicrobial activity of propolis water extracts prepared from three different raw propolis samples were investigated. The total flavonoid contents (TFC) and total phenolic content (TPC) of the extracts were measured. It was determined that the TPC and TFC ranged from 5.87±0.36 to 20.47±1.46 mg GAE g-1, and 0.48±0.04 to 2.10±0.22 mg QUE g-1, respectively. The antimicrobial activity of the extracts against 14 microorganisms and their effect against the Pseudomonas aeruginosa biofilm were investigated. Additionally, anti-quorum sensing and anti-swarming activities of the extracts were tested. The antiviral activity of the extracts was examined by MTT and qRT-PCR methods. The water extracts of propolis samples included in the study did not show antimicrobial, anti-swarming, anti-quorum sensing, and anti-viral activities. However, the extracts were found to have strong anti-biofilm activities. The results show that aquatic propolis extracts can be evaluated in the treatment of biofilms.