Antibiogram Of Bacteria Research Articles

Bacterial Culture Results and Antibiograms in Chronic Rhinosinusitis Patients in Mekong Delta, Vietnam

Background The inappropriate use of antibiotics for chronic rhinosinusitis, exceeding established guidelines, is a growing concern in Vietnam. This practice is contributing to the emergence and spread of antibiotic-resistant bacteria. Objective This study aimed to evaluate the results of culture and antibiogram of bacteria on patients in the Mekong Delta, Vietnam, who were treated for chronic rhinosinusitis in 2022-2023. Methods It is a cross-sectional study, including 113 patients who came for treatment with a diagnosis of chronic rhinosinusitis at Can Tho University of Medicine and Pharmacy Hospital, Mekong Delta, Vietnam, from 2022-2023. The bacterial cultures were identified, and their antibiograms were performed in the Microbiology laboratory of Can Tho University of Medicine and Pharmacy. Statistical analysis was performed with SPSS version 20.0. Results The rate of bacterial growth was 97.35%. The cultured bacterial composition had 8 genera, 22 species, and 116 samples, of which 2 bacterial species were cultured, accounting for 5.45%. Antibiogram showed that Streptococcus pneumonia was resistant to cefaclor (83.33%), cefuroxime (100%), trimethoprim+ sulfamethoxazole (100%), and susceptible to chloramphenicol (100%) and vancomycin (100%). Streptococcus haemolyticus was resistant to amoxicillin+clavulanic acid (85.71%) and ampicillin (100%) and susceptible to linezolid (100%) and rifampin (100%). Streptococcus epidermidis was resistant to amoxicillin+clavulanic acid (82.35%), ampicillin (100%), penicillin (100%) and susceptible to linezolid (91.18%) and rifampin (88.24%). Streptococcus aureus was resistant to amoxicillin+clavulanic acid (95%) and ampicillin+sulbactam (90%), and susceptible to linezolid (90%) and synercid (95%). Pseudomonas aeruginosa was resistant to amikacin (100%), meropenem (100%) and tobramycin (90%). Conclusion This study offers some useful information to doctors in the Mekong Delta and across Vietnam. It can be used as a good orientation in choosing the most appropriate antibiotics for treating patients with chronic rhinosinusitis. It is important to be conscious of the current trend of the bacteriological profiles and to regulate the antibiotic treatment regime to improve the effectiveness and reduce antibiotic resistance rate.

ENUMERATION AND DETERMINATION OF ANTIBIOGRAM OF BACTERIA ISOLATES FROM HAND-DUG WELL WATER IN BOSSO METROPOLIS, NIGERIA

Regular water supply surveillance is crucial in low-income nations like Nigeria, where households often rely on alternative sources, predisposing preventable water-borne diseases. Therefore, this study determined the occurrence and antibiogram of bacteria in hand-dug well water in Bosso metropolis, Nigeria. Exactly 10 well water samples were collected and processed for bacteria isolation and identification using standard microbiological techniques. The antibiotic sensitivity was determined via Kirby-Bauer disc diffusion techniques. The total viable bacteria count ranged from 0.45×103 cfu/mL to 2.15×104 cfu/mL, while the total coliform bacteria count ranged from 0.27×103 cfu/mL to 8.91×104 cfu/mL. Out of the 18 different bacteria isolated, Pseudomonas species had the highest occurrence (26.9%), and the least (3.9%) was observed for each of Shigella sp., Klebsiella sp., and Staphylococcus aureus. Also, 7 out 26 bacterial isolates isolated were multidrug-resistant. Escherichia coli, Shigella sp., Salmonella sp., Pseudomonas sp., and Klebsiella sp. were susceptible (100%) to ciprofloxacin and ofloxacin, though resistant (100%) to ampicillin and amoxycillin/clavulanate. All the Gram-negative bacteria isolates except Salmonella sp. were susceptible to gentamycin. Similarly, Pseudomonas sp., Proteus sp., and Salmonella sp. exhibited varied resistance to nitrofurantoin (71–100%), cefuroxime (50–100%), and ceftazidime (42.9–100%). Bacillus sp., Enterococcus sp., Staphylococcus sp., and Streptococcus sp. were resistant to cloxicillin and amoxycillin/clavulanate. In addition, Bacillus sp. (100%) and Enterococcus sp. (100%) were resistant to ciprofloxacin while remaining susceptible to ofloxacin and amoxycillin/clavulanate. Our findings highlight the urgent need for enhanced water quality and public health measures in the study areas...

Microbial Analysis and Antibiogram of Bacteria and Fungi Isolated from Meat Sellers Tables in Dutsin-Ma Metropolis, Katsina State

The goal of the research project was to carry out microbiological analysis and antibiogram of bacteria and fungi associated with meat vendor tables in the city of Dutsin-Ma. This study made use of twenty samples in total. Five distinct locations at Wednesday Market and Darawa were chosen, and two samples from each table were taken using swab sticks. For analysis, the samples were then brought to the Federal University of Dutsin-ma microbiology laboratory department. The agars that were initially employed to isolate the bacteria and fungus were MacConkey, nutrient, mannitol salt, bile esculin, salmonella-shigella, eosin methylene blue, and potato dextrose agars. Bacterial species were identified using gram staining and biochemical tests while lactophenol cotton blue staining was used for the identification of fungal species. Bacterial isolates identified include Escherichia coli, Salmonella species, Klebsiella pneumonia, Bacillus subtilis, Streptococcus species and Staphylococcus aureus while the fungal isolates are Aspergillus flavus, A. niger, A. fumigatus, and Oidiodendron griseum. The antimicrobial susceptibility test findings showed that while some of the bacterial isolates were responsive to certain drugs, others were resistant. The results of this study's investigations therefore demonstrated how heavily polluted meat vendors' tables are with a wide variety of bacteria. This can be explained by the unsanitary procedures used in the meat sale industry as well as other environmental issues. Therefore, it is imperative that the government authorities tasked with these duties make sure that the vendors of these meat products adhere to standard and hygienic procedures. Raising public knowledge of the risks of foodborne illnesses associated with eating raw meats is another important goal of public health awareness campaigns.

Prevalence and antibiogram of bacteria causing urinary tract infection among patients with chronic kidney disease.

Identifying and appropriately managing urinary tract infections (UTIs) among chronic kidney disease (CKD) patients are essential to reduce further disease complications and economic burden. Hence, this study aims to determine the prevalence of UTIs among CKD patients and study the antibiogram of the bacterial isolates. Four hundred eighty-two clean catch midstream urine samples were collected from CKD patients during the study period. The samples were cultured, and bacteria were isolated using standard microbiological techniques. Antibiotic susceptibility testing was performed by the Kirby-Bauer disc diffusion method following the Clinical and Laboratory Standards Institute guidelines. Of the 482 CKD patients, 15.8% were culture positive, and the majority was elderly aged group population. Most bacterial isolates were Escherichia coli 50%, followed by Pseudomonas aeruginosa 15.80%, Enterococcus species 15.80%, and Klebsiella pneumoniae 11.84%. The majority of bacteria were found to be resistant to beta-lactam antibiotics, ampicillin (94.67%), ceftriaxone (89.04%), cefotaxime (87.5%), and ceftazidime (84.0%), while polymyxin, colistin, vancomycin, meropenem, and imipenem were the most sensitive antibiotics. In our study, higher levels of antibiotic resistance were observed among urinary isolates. Therefore, our findings suggest clinicians to choose better antibiotic options to treat UTIs among CKD patients.

Isolation and Antibiogram of bacteria and fungi associated with tattoo equipment used in port Harcourt metropolis

Isolation and Antibiogram of bacteria and fungi associated with tattoo equipment used in port Harcourt metropolis

Bacteriological Profile and Antibiogram of Bacteria in Sputum Culture of Iraqi Patients: A retrospective study

Background: viruses are responsible for a large proportion of lower respiratory tract infections (LRTIs). Other causes of LRTIs are bacteria: Streptococcus pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, and Staphylococcus aureus being the most common. Sputum samples are commonly used in the microbiological laboratory for diagnosing lower respiratory infections. Objective: The aim of this study to evaluate the causative bacteria and antibiotics sensitivity in culture of sputum samples. Patients Methods: A retrospective study performed in the microbiology department of Al Immamin Al Kahdimin Medical laboratory in Baghdad. The results of sputum cultures collected from the files between 2016 and 2019. A total number of 131 included in the study of adults and both sexes. Organisms were identified and tested for the antibiotic susceptibility did for selected cases which ordered by the doctor needed. Results: The number of 131 were enrolled. The age of patients was between 17-85 years with mean age 46.69. The higher incidence of patients between ages 51-60 years (21.4 %). The female were 40.5%, the male 59.5%. 65 (49.62%) patients from the medical ward, 50 (38.17) from respiratory care unit (RCU). Acinetobacter spp was the most common bacteria isolated, in forty four (33.59%) cases, which was resistant to most antibiotics. followed by Streptococcus pneumonia (22.90%), Pseudomonas aeruginosa (16.03%),Escherichia coli in eleven (8.40) cases, with variable antibiotics sensitivity and resistance. Conclusion: sputum culture and sensitivity may help in identifying the organism and choosing the antibiotic, which may be resistant to many drugs as in Acinetobacter spp.

INVESTIGATION OF THE ANTIBIOTIC RESISTANCE: THE CASE OF BUU DIEN GENERAL HOSPITAL IN HO CHI MINH CITY

Objective: In Vietnam, antibiotic resistance has been gained the attention of medical professionals in antibiotic use management. This study aimed to investigate the antibiotic resistance among hospital-acquired infections at Buu Dien General Hospital in Ho Chi Minh City in the period of 01-12/2017.
 Methods: This cross-sectional descriptive study was conducted on the retrospective data of all antibiograms of bacteria isolated from hospital-acquired infections at Buu Dien General Hospital in Ho Chi Minh City in the period of 01-12/2017 to investigate the antibiotic resistance. Characteristics of antibiotic resistance were described by frequency and percentage of types of bacteria isolated and antibiotics being resistant.
 Results: A total of 179 isolates were collected during the period 01-12/2017, of which E. coli was the most commonly isolated pathogen (41.3%). The highest prevalent infections were in the skin and mucosa; respiratory tract; and urinary tract (34.6%; 32.4%; and 27.9%). The antibiotic susceptibility testing used 21 types of antibiotics. Among them, S. aureus was 82% resistant to clindamycin and 75% resistant to cefuroxime; the Proteus resistance percentages to amoxicillin/clavulanic, second-generation cephalosporins, ciprofloxacin and fosfomycin varied from 50 to 93%; Pseudomonas was 92% resistant to fosfomycin and 62% resistant to ceftazidime; A. baumannii was resistant to most classes of agents used (50-75%). Both E. coli and Klebsiella were highly resistant to gentamicin, amoxicillin, ciprofloxacin, 2nd and 3rd generation cephalosporin’s. Polymyxin B-resistant Proteus cultures were detected at 67%.
 Conclusion: The study described the antibiotic resistance situation of hospital-acquired bacteria at the Buu Dien General Hospital from 01-12/2017. This information will aid physicians to select proper antibiotics for their patients in the next period.

Antibiogram of bacteria isolated from dogs suffering from otitis externa

This study was conducted to isolate the bacterial organisms associated with otitis externa in dogs and to determine their antibiogram. Paired ear swab samples were collected from 56 dogs suffering from clinical signs of itching, head shaking, off smelling odour from ear, unilateral or bilateral otorrhoea, etc., which are indicative of otitis externa. On cultural examination, 47 swabs were found positive for bacterial infection with 61.70% samples showing mono-infection, whereas mixed infection was observed in 38.29% samples. A total of 65 bacterial isolates were recovered with maximum frequency of Staphylococcus spp. (53.84%) followed by Streptococcus spp. (18.46%), Pseudomonas spp. (12.30%), Proteus spp. (7.69%), Escherichia coli (6.15%). Least isolated organism was Corynebacterium spp. (1.53%). Irrespective of the isolated organisms, the overall antibiogram pattern revealed sensitivity towards various antibiotics in the range of 31.25–74.35%. Amongst 19 tested antibiotics, Cefoperazone was found to be most sensitive whereas Septran to be least sensitive antibiotic.

Spectrum and Antibiogram of Bacteria in Chronic Suppurative Otitis Media and Biofilm Formation

Context nbsp Chronic suppurative otitis media CSOM is a common infection having a high prevalence rate of in India and is one of the leading causes of conductive deafness particularly in the adolescent age group CSOM is frequently associated with multidrug resistant organisms with the ability to form biofilm which is an important virulence factor and results in treatment failure Aims nbsp The main objectives of the study are to identify the spectrum of bacteria associated with CSOM their antibiotic sensitivity pattern and to detect the biofilm formation Methods nbsp This was a prospective cross sectional study at a tertiary care hospital Patients upto the age of having otorrhea for more than weeks and attending the ENT outpatient department were included Samples of pus were collected from the deeper aspect of external auditory meatus and processed using standard techniques for culture and sensitivity Biofilm detection was done Results were compiled and statistically analysed Results nbsp Sixty samples were processed and yielded positive cultures Staphylococcus aureus was the predominant organism followed by Pseudomonas aeruginosa Biofilm formation was seen in per cent of the organisms Biofilms were formed predominantly by Staphylococcus aureus per cent Conclusion nbsp Multidrug resistance has become increasingly common amongst the causative organisms of CSOM this trend being particularly evident among biofilm producers Therefore screening for biofilm formation along with the usual antibiogram needs to be performed as a routine procedure in CSOM to effectively manage the situation by choosing an appropriate modality of treatment nbsp

Virulence Factors and Antibiogram of Bacteria from Fresh Aquatic Produce from Open Air Market Centre in Okepedi, Itu, Akwa Ibom State

Virulence Factors and Antibiogram of Bacteria from Fresh Aquatic Produce from Open Air Market Centre in Okepedi, Itu, Akwa Ibom State