Antibacterial Gauze Research Articles

Mussel-inspired modified regenerated cellulose as tissue adhesive and antibacterial gauze: A promising approach for rapid hemostasis in non-compressible hemorrhage

Mussel-inspired modified regenerated cellulose as tissue adhesive and antibacterial gauze: A promising approach for rapid hemostasis in non-compressible hemorrhage

Nanoclay Mediated Self‐Promoted Reactive Oxygen Species Releasing Enables Efficient Antimicrobial

AbstractReactive oxygen species (ROS) releasing materials are increasingly used in nanomedicines due to their effectiveness against a broad spectrum of microbes. However, most ROS‐releasing materials rely on external stimuli such as photoirradiation, either through photodynamic or photocatalytic processes, which limits their practical applications. Herein, this work presents a novel nanoclay mediated self‐promoted ROS releasing material called oxygen vacancies‐rich ZnO/kaolinite (Ov‐rich ZnO/Kaol). Both experiments and density functional theory (DFT) calculations reveal that the introduction of kaolinite increases the content of Ov in ZnO, and the change in the electronic structure promotes the process of oxygen adsorption and activation, resulting in the generation of ·O2−through oxygen reduction without photoirradiation. Molecular dynamics simulations confirm that Ov enhances the interaction between the material and bacterial membrane, promoting the bacterial‐killing effect of ·O2−. More importantly, Ov‐rich ZnO/Kaol is successfully prepared on a pilot scale and used to manufacture antibacterial gauze, which showed at least 99% antibacterial activity against both Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in the absence of light. The as‐prepared material also exhibited satisfactory biocompatibility and biosafety. This method can offer a more benign approach to address the limitations of traditional photoirradiation‐dependent photosensitizers for antimicrobial gauze.

Rapid and Eco‐Friendly Preparation of Antibacterial Gauze by Alkali‐Acid Redeposition of Quercetin on Gauze Fiber and Subsequent Ultraviolet‐Assisted Reduction of Silver In Situ

AbstractRapid and eco‐friendly preparation of biomedical materials is significant for practical applications. This study proposes a simple two‐step method for preparing silver nanoparticles (AgNPs)‐functionalized gauze dressings (Ag‐Q‐G) with antibacterial activity in a green and time‐efficient manner that requires <10 min. A smooth and uniform quercetin coating is formed on the gauze fibers using alkali–acid redeposition for 10 s. Subsequently, AgNPs are rapidly formed (in 5 min) and firmly deposited on the quercetin‐coated gauze by ultraviolet irradiation in an AgNO3 solution. The in vitro results indicate that Ag–Q–G exhibits excellent antibacterial activity, with a rate of 99.57% ± 0.52% against Staphylococcus aureus and 95.59% ± 1.50% against Escherichia coli, while maintaining acceptable cytocompatibility. An animal study reveals that Ag‐Q‐G reduces infection and promotes wound healing in a rat dorsal total cortical wound model. This approach provides a practical option for the commercial production of antibacterial gauze dressings.

A facile strategy to fabricate silver-functionalized superhydrophobic cotton fabrics with long-term antibacterial properties

Silver-functionalized textiles, as the most common protective medical materials, have attracted considerable attention. However, poor antibacterial durability and relatively tedious preparation process limit their applications. In this study, a kind of multifunctional cotton fabric was prepared through simultaneous dip-coating of silver-silica hybrid nanoparticles (Ag-MSNs) and fluorinated MSNs (F-MSNs), providing excellent antifouling ability due to superhydrophobicity and long-term antibacterial properties from the sustained release of Ag ions (Ag+). Detailed studies were performed to evaluate their structure and protective performance, especially the long-term antibacterial properties of the obtained fabrics were tested by the inhibition zone experiment for 25 d. The as-prepared fabrics showed good non-wetting properties and sustained antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Further washing and abrasion experimentations demonstrated that the as-prepared fabrics had stable hydrophobicity and antibacterial properties, much more reliable than commercial antibacterial gauze. Considering the inexpensive and simple preparation process as well as long-term and efficient effect for sterilization, the strategy paves a new and facile way for the fabrication of multifunctional fabrics with robust hydrophobic and antibacterial activity.

Antibacterial gauze based on the synergistic antibacterial mechanism of antimicrobial peptides and silver nanoparticles

Antibacterial gauze is commonly used to prevent wound inflammation and infection. In this study, a novel antibacterial gauze was prepared using antimicrobial peptides and silver nanoparticles, achieving a wide antimicrobial spectrum and increased antimicrobial properties. The properties of the antibacterial gauze were analyzed by scanning electron microscopy, energy-dispersive spectrometry, weight gain rate test, Fourier transform infrared spectroscopy, antibacterial test, and cell activity test. The results of energy-dispersive spectrometry and Fourier transform infrared spectroscopy suggested that the antimicrobial peptides and silver nanoparticles were loaded on the surface of the gauze. The prepared gauze exhibited excellent antibacterial properties against Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, and Bacillus cereus due to the synergistic antibacterial effect of the antimicrobial peptides and silver nanoparticles. Compared with antibacterial gauze loaded with high concentrations of silver nanoparticles, the gauze prepared here has better antimicrobial properties and no obvious cytotoxicity to cells. These results indicate that this antibacterial gauze, with good compatibility, is an attractive candidate for application in wound care and wound healing.

Research on nursing of common open trauma under nano antibacterial technology

Objective: The thesis studies the antibacterial properties of cyanoacrylate antibacterial adhesive modified by 6% nano-silica. Method: The paper used the filter paper method to test the bacteriostatic performance of 6% nano-silica/antibacterial glue against Staphylococcus aureus (ATCC29213). The unmodified antibacterial glue was used as a control to measure the diameter of the inhibition zone. The rat trauma animal model was made, and the wound was treated with 6% nano-silica antibacterial glue and gauze. The general condition of the wound was observed, and the arterial blood routine and the pathological section of the wound tissue were performed to detect the white blood cell count. Results: The diameter of the inhibition zone on the 5th day of the 6% nano-silica/antibacterial glue group was (28.61 ± 0.91) mm, and that of the antibacterial glue group was (28.24 ± 2.69) mm. There was no significant difference between the two groups (P > 0.05). In animal experiments, in blood routine and wound tissue pathological sections, the infection of the wound treated with 6% nano-silica/antibacterial glue was lower than that of the gauze dressing group (P < 0.05). Conclusion: The antibacterial glue modified by 6% nano-silica has better antibacterial properties (anti-Staphylococcus aureus).

Using carboxymethyl gum from Tamarindus indica and Cassia fistula seeds with Chromolaena odorata leaf extract to develop antibacterial gauze dressing with hemostatic activity.

Background and purpose:Gauze dressing is a barrier against microbial infection in wounds. The seed gums of Tamarindus indica and Cassia fistula are abundant in tropical countries; we used them as a coating material of cotton gauzes to improve the liquid absorption ability. Moreover, Chromolaena odorata leaf extract was loaded on the gums for antibacterial gauze dressing with hemostatic activity.Experimental approach:Crude gums were extracted from T. indica and C. fistula seeds and carboxymethyl gums were then derived and chracterized. C. Odorata ethanolic extract was also prepared by maceration and its antimicrobial and blood clotting activities were determine coated gauze dressing containing different concentrations of carboxymethyl gum was prepared in the presence of various concentrations of C. odorata extract. Then, the physical properties, antibacterial activity, and skin-irritating effects of the coated gauze were analyzed.Findings/Results:The results indicated that the amount of carboxymethyl gum affected the physical properties and absorption capacity of the coated gauze. C. odorata extract exhibited better bactericidal activity against Staphylococcus aureus than against Escherichia coli. The blood clotting effects of C. odorata extract indicated that it had dramatic hemostatic efficacy. The coated gauze exhibited bactericidal activity against S. aureus. In the human skin irritation test, the coated gauze caused no adverse effects on human skin.Conclusion and implication:Coated gauze has the potential to serve as a prototype for primary hemostasis in first aid for opened wounds such as abrasions and lacerations.

Lotus leaf inspired antiadhesive and antibacterial gauze for enhanced infected dermal wound regeneration

Gauze is the leading dressing for wound management. However, infection and tissue adhesion pose serious challenges to the application of gauzes. Herein, inspired by the self-cleaning property of lotus leaf, we develop a novel antiadhesive and antibacterial gauze. The gauze, named Lotus@Gauze, is fabricated by decorating an ordinary medical gauze sequentially with polydopamine, perfluorocarbon and silver nanoparticle to obtain a lotus leaf-mimic surface structure. Lotus@Gauze exhibits excellent antiadhesive and antibacterial effects, and its antibacterial ability is enhanced under NIR irradiation due to the photothermal effect of polydopamine. Furthermore, in vivo results reveal that, in comparison with clinically available antiadhesive or antibacterial gauzes, Lotus@Gauze shows better efficacy in promoting infectious wound regeneration due to its low tissue adhesion and effective bactericidal activity. Overall, this study explores a new attempt to overcome the challenge of tissue adhesion and infection by integrating antiadhesive and antibacterial properties into one piece of simple gauze.

Antimicrobial effects of positively charged, conductive electrospun polymer fibers.

In recent years, electrospun polymer fibers have gained attention for various antibacterial applications. In this work, the effect of positively charged polymer fiber mats as antibacterial gauze is studied using electrospun poly(caprolactone) and polyaniline nanofibers. Chloroxylenol, an established anti-microbial agent is used for the first time as a secondary dopant to polyaniline during the electrospinning process to make the surface of the polyaniline fiber positively charged. Both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli are used to investigate the antibacterial activity of the positively charged and uncharged polymer surfaces. The results surprisingly show that the polyaniline surface can inhibit the growth of both bacteria even when chloroxylenol is used below its minimum inhibitory concentration. This study provides new insights allowing the better understanding of dopant-based, intrinsically conducting polymer surfaces for use as antibacterial fiber mats.

Antibacterial gauzes are effective in preventing infections after percutaneous endoscopic gastrostomy placement: a retrospective analysis.

The most common complication after percutaneous endoscopic gastrostomy (PEG) placement is peristomal wound infection (up to 40% without antibiotic prophylaxis). Single-dose parenteral prophylactic antibiotics as advised by current guidelines decrease the infection rate to 9-15%. We assume a prolonged effect of local antibiotic treatment with antibacterial gauzes. This study is the first to describe the effect of antibacterial gauzes in preventing infections in PEG without the use of antibiotics. A retrospective data analysis was carried out of all patients with PEG insertion between January 2009 and October 2014 in the Catharina Hospital Eindhoven. Data include placement and the period of the first 2 weeks after PEG placement, and long-term follow-up. All patients received a locally applied antibacterial gauze polyhexamethylene biguanide immediately following PEG insertion for 3 days. No other antibiotics were administered. The main outcomes were wound infection, peritonitis, and necrotizing fasciitis; secondary outcomes included other complications. A total of 331 patients with only antibacterial gauzes were analyzed. The total number of infections 2 weeks after PEG insertion was 9.4%, including 8.2% minor and 1.2% major infections (peritonitis). No wound infection-related mortality or bacterial resistance was found. Costs are five times lower than antibiotics, and gauzes are more practical and patient friendly for use. Retrospectively, antibacterial gauzes are at least comparable with literature data on parenteral antibiotics in preventing peristomal wound infection after PEG placement, with an infection rate of 9.4%. Rates of other complications found in this study were comparable with current literature data.

Lysozyme-immobilized electrospun PAMA/PVA and PSSA-MA/PVA ion-exchange nanofiber for wound healing

This research was aimed to develop the lysozyme immobilized ion-exchange nanofiber mats for wound healing. To promote the healing process, the PSSA-MA/PVA and PAMA ion-exchange nanofiber mats were fabricated to mimic the extracellular matrix structure using electrospinning process followed by thermally crosslinked. Lysozyme was immobilized on the ion-exchane nanofibers by an adsorption method. The ion-exchange nanofibers were investigated using SEM, FTIR and XRPD. Moreover, the lysozyme-immobilized ion-exchange nanofibers were further investigated for lysozyme content and activity, lysozyme release and wound healing activity. The fiber diameters of the mats were in the nanometer range. Lysozyme was gradually absorbed into the PSSA-MA/PVA nanofiber with higher extend than that is absorbed on the PAMA/PVA nanofiber and exhibited higher activity than lysozyme-immobilized PAMA/PVA nanofiber. The total contents of lysozyme on the PSSA-MA/PVA and PAMA/PVA nanofiber were 648 and 166 µg/g, respectively. FTIR and lysozyme activity results confirmed the presence of lysozyme on the nanofiber mats. The lysozyme was released from the PSSA-MA/PVA and PAMA/PVA nanofiber in the same manner. The lysozyme-immobilized PSSA-MA/PVA nanofiber mats and lysozyme-immobilized PAMA/PVA nanofiber mats exhibited significantly faster healing rate than gauze and similar to the commercial antibacterial gauze dressing. These results suggest that these nanofiber mats could provide the promising candidate for wound healing application.

Antibacterial and anti-mildew behavior of chitosan/nano-TiO2 composite emulsion

A novel chitosan/nano-TiO2 composite emulsion (CTCE) was prepared by inverse suspension technol- ogy, and its multi-function properties were studied. As can be seen from the results, the gauze treated with chitosan/ nano-TiO2 composite emulsion showed excellent antibacterial activities against Escherichia coli, Aspergillus niger and Candida albicans that the bactericidal ratios could reach 99.96%, 100% and 78.3% after 24 h, respectively. At the same time, the chitosan/nano-TiO2 composite emulsion could really kill the bacteria, not just inhibit its growth, and the bac- tericidal ratio for E. coli could reach 70.3% after 1 h. The bactericidal ratio for E. coli reached 98.8% at 27 o C com- pared with 58.7% at 18 o C after 8 h due to the activity of bacteria being weakened at low temperature. Furthermore, the antibacterial ability of antibacterial gauze was hardly influenced with the increase of storage time, which could be reused for up to 8 times without loss of antibacterial ability.