Results Of Antibacterial Activity Test Research Articles

Antibacterial and antioxidant activities in various parts of Artocarpuslacucha Buch. Ham. ethanolic extract.

Artocarpus lacucha is an endemic plant to North Sumatera, Indonesia. This plant has pharmacological activities, including acting as an antioxidant and antibacterial. The aim of the present study was to analyze the antibacterial and antioxidant activities, and determine the flavonoid compounds from four parts of A. lachuca, namely leaves, barks, twigs and fruits. Antioxidant activity was investigated using the 2,2-diphenyl 1-picrylhydrazyl (DPPH) and cupric reducing antioxidant capacity (CUPRAC) methods. Antibacterial activity was analyzed using disk diffusion and microdilution methods. Several flavonoids, such as luteolin-7-O-glucoside, rutin, quercetin, kaempferol and apigenin, were determined using high performance liquid chromatography. Based on the antioxidant activity test results using the DPPH method, the bark ethanolic extract provided the highest antioxidant capacity, while the CUPRAC method indicated that the twig ethanolic extract had the highest antioxidant capacity. The antibacterial activity test results demonstrated that at a low concentration of 750 µg/disk the bark ethanolic extract obtained the highest inhibition zone and minimum inhibitory concentration level against six of nine pathogenic bacteria. Therefore, A. lachuca bark ethanolic extract could be potentially developed as antioxidant and antibacterial agents.

Herbal active ingredient-loaded poly(ω-pentadecalactone-co-δ-valerolactone)/gelatin nanofibrous membranes

Recently, there has been an accelerating interest in novel biocompatible wound dressings made of nano-sized materials, especially nanofibers. Electrospun nanofibers provide high surface area and mimic the extracellular matrix which enhances biocompatibility. Besides, nanofibrous structures have high active ingredient loading capacity as a result of their high surface-to-volume ratio and porosity. In the present study, curcumin-loaded poly(ω-pentadecalactone-co-δ-valerolactone)/gelatin (PDL-VL/Gel) nanofibrous membranes were fabricated to be used for healing skin wounds. Poly(ω-pentadecalactone-co-δ-valerolactone) copolymer has been enzymatically synthesized in previous studies, thus it improves the originality of the membrane. It was aimed to obtain a synergetic effect and increase the novelty of the work by blending synthetic and natural polymers. Moreover, it was preferred to provide antibacterial activity by the incorporation of a herbal ingredient (curcumin) as a natural alternative to commercial antibiotics. Varied amounts of curcumin (5–25 %, w:v) were electrospun together with PDL-VL/Gel (equal volume ratio) polymer blend (fiber diameters ranged between 554 and 1074 nm) and several characterizations (morphological and molecular structure, wettability characteristics, and thermal behavior) were applied to examine the curcumin incorporation. Afterwards, in vitro curcumin release studies were carried out and mathematical modeling was applied to release data to clarify the transport mechanism. Curcumin release profiles comprised of an initial burst release in the first hour followed by a sustained release through 24 h. Based on the antibacterial activity test results, 15 % curcumin loading ratio was found to be sufficient for the treatment of skin wounds infected by Gram-negative (E. coli) and Gram-positive (S. aureus and B. subtilis) bacteria. Additionally, nanofibrous membranes did not lead to cytotoxicity, and curcumin content further enhanced the viability of fibroblasts. Thus, the presented antibacterial nanofibrous membrane is suggested to be applied for the treatment of wound infections and accelerating the healing process.

Formulation and Antibacterial Activity Test of Salmonella thypimurium and Lactobacillus acidophilus in Microencapsulation of Mangosteen Peel Extract

Xanthone compounds in the ethanol extract of mangosteen peel have antibacterial activity against both pathogenic and beneficial bacteria. The purpose of this study was to examine the effect of various formulations of ethanol extract of mangosteen peel and maltodextrin from palm starch on Salmonella thypimurium and Lactobacillus acidophilus. This research was conducted by culturing the bacterial cultures of S. thypimurium and L. acidophilus on nutrient agar (NA) media in petri dishes according to the Kirby-Bauer method. The NA media that had been made in the petri dish was then made wells to test each treatment as much as 10 µL/disk at various concentrations (100, 75 and 50%). The formulations tested in this study consisted of: T1 (30% ethanol extract of mangosteen peel + 70% maltodextrin), T2 (40% ethanol extract of mangosteen peel + 60% maltodextrin), T3 (50% ethanol extract of mangosteen peel + 50% % maltodextrin), T4 (60% ethanol extract of mangosteen peel + 40% maltodextrin), T5 (70% ethanol extract of mangosteen peel + 30% maltodextrin), T6 (ethanol extract of mangosteen peel only), T7 (maltodextrin only), T8 (positive control containing 100 ppm bacitracin) and T9 (negative control/no treatment). The results of each sample were observed by measuring the sample inhibition zone on the growth medium that had been prepared. Data from the antibacterial activity test results were then analyzed using one-way ANOVA followed by Duncan's test. The results showed that the ethanol extract of mangosteen peel and maltodextrin had a significant effect (P<0.05) on the antibacterial activity of Salmonella thypimurium and Lactobacillus acidophilus. The results of this study indicated that the formulation of ethanol extract of mangosteen peel and maltodextrin had the ability as an antibacterial against Salmonella thypimurium and Lactobacillus acidophilus at a medium level.

Antibacterial Activity of Bitter Drugs from Lingga Malay Ethnic in Riau Archipelago Against Bacterial Causing Skin and Diarrhea Diseases

Infection is the major threat to humanity caused by infectious agents. The most common infectious agent of human is the cause of skin and diarrhea diseases. Melayu Lingga people uses alternative medicine called obat pahit potion to treat skin and diarrhea diseases and mixes by Praktisi Obat Tradisional (POT). The purpose of this research was to determine antibacterial activity of the methanol extract from obat pahit potion against bacteria that cause skin diseases (Staphylococcus aureus ATCC 12600, Staphylococcus epidermidis ATCC 12228, Pseudomonas aeruginosa ATCC 10145) and diarrhea (Escherichia coli ATCC 11775, Salmonella typhi ATCC 14028, Shigella flexneri ATCC 12022) by using disc diffusion method with variation of extract concentration (0.75 gr/ml, 0.5 gr/ml, and 0.25 gr/ml). Antibacterial activity was determined by measuring the inhibitory zone formed around the disc. Based on the result of antibacterial activity test, the largest inhibitory zone was found in S.aureus ATCC 12600, S.epidermidis ATCC 12228 and P.aeruginosa ATCC 10145 shown in POT Musai at 0.25 mg/ml concentrations with value 6,61 mm; 6,10 mm; and 10,11 mm. Meanwhile, antibacterial activity against E.coli ATCC 11775 shown in TMP Musai at 0.5 gr/ml concentration with value 4,47 mm. Antibacterial activity against S.typhi ATCC 14028 and S.flexneri ATCC 12022 shown in TMP Musai at 0.25 gr/ml concentration with value 8,20 mm and 11,73 mm.

Formulasi Tablet Effervescent Ekstrak Sirih Cina (Peperomia pellucida L.) Dan Uji Aktivitas Terhadap Antibakteri Staphylococcus aureus

Chinese Betel herb (Peperomia pellucida L.) has flavonoid and saponin content compounds that can inhibit bacterial growth. The innovation of effervescent tablets used as disinfectants is not widely known in the Indonesian market. This preparation has the advantage of being easy to manufacture as a disinfectant. Chinese betel herb extract is formulated into an effervescent tablet preparation. This study aims to determine the influence on variations in the levels of binders and fillers in the physical properties of effervescent tablet preparations and determine the activity in the effervescent tablet formula of Chinese betel extract (Peperomia pellucida L.) in inhibiting the growth of Staphylococcus aureus bacteria. Chinese betel herb extract is obtained through a maceration extraction process using methanol solvent for 5 days. The preparation of effervescent tablets is made 3 formulas, namely the concentration of binding substances 10%, 13%, 15%. Physical tests of tablet preparations include organoleptic tests, tablet hardness tests, tablet soluble time tests, size uniformity tests, weight uniformity tests,, fragility tests and antibacterial activity tests against Staphylococcus aureus with disc paper diffusion methods.The best formula tablets are formula I (PEG 10%) with uniformity of weight 525±17,014, tablet hardness 3,452±1,172, tablet fragility 0.264%, soluble time 1 minute 6 seconds, uniformity size 3.33±0.06. The results of antibacterial activity tests showed weak inhibitory power with an average formula value of 4.51±0.02.

Antibacterial Activity of Ethanol Extract of Portulaca oleracea L. Herb from Various Extraction Methods Against Salmonella typhimurium

Salmonella typhimurium bacteria could cause gastroenteritis and its growth could be controlled by the active compounds from natural products, which is Portulaca oleracea L. herb. Portulaca oleracea contained tannin, saponins and flavonoids compounds which had different characteristics towards temperature extraction. This study aims to determine the antibacterial activity of ethanol extract of the Portulaca oleracea herb from various extraction methods against S. typhimurium bacteria. Extraction of Portulaca oleracea herb was carried out with four variations methods which were the cold method (maceration and percolation) and heat method (soxhlet and refluxs) using 96% ethanol solvent. The four types of extract were tested for their antibacterial activity by disk diffusion at concentrations of 30%, 35%, 40%, 45% and 50% (b/v). The positive control was chloramphenicol 30 µg/disk, while the negative control was DMSO solvent. The results of antibacterial activity test in the form of zone of inhibition were statistically analyzed by Two Way Anova. The results showed that the ethanol extract of the Portulaca oleracea herb from various extraction methods had antibacterial activity against S. typhimurium. There was a significantly difference in the antibacterial activity of ethanol extract of the Portulaca oleracea herb obtained from the reflux method with other methods (maceration, percolation and soxhlet) against S. typhimurium.
 Keywords: ethanol extract of Portulaca oleracea L. herb, antibacterial, Salmonella typhimurium, various extraction methods

UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn) on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

Effectiveness of Methanol Extract of Moringa oleifera Lam. Leaf as Antibacterial Drug to Bacterial Triggers of Urinary Tract Infections In vitro

Background: Urinary tract infections (UTIs) are a severe public health problem and are caused by a range of pathogens, but most commonly by Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterococcus faecalis and Staphylococcus saprophyticus. High recurrence rates and increasing antimicrobial resistance among uropathogens threaten to greatly increase the economic burden of these infections. This study aimed to measure the lowest concentration of Moringa oleifera Lam. Leaf that did not show any growth of the tested microorganisms.
 Methods: The antibacterial activity test was adapted from the CLSI broth microdilution assay, with minor modifications, were used for the evaluation of the antibacterial activity of M. oleifera extracts against tested bacteria. Three concentrations higher than the MIC standard were cultured on the MHA. The lowest concentration on agar which was not found any growth of the bacterial colonies was determined as the MBC.
 Results: The antibacterial activity test results showed that Moringa leaf extract could inhibit bacterial growth. Inhibition of bacterial growth based on extract concentration. For E. coli bacteria, the results showed the highest absorbance value at 100mg/ml concentration (2.86±0.02); even when diluted to 3,13 mg/ml (1.08±0.01), the moringa leaf extracts could inhibit the E. coli growth, although the value did not differ greatly from that of the negative control (0.13±0.06). The same as E. coli, the antibacterial activity test results showed that moringa leaf extract could inhibit others bacteria growth of UTIs.
 Conclusions: Methanol extract of Moringa oleifera leaf can inhibit and bactericide the growth of urinary tract infections bacteria.

Identification of Bacterial Symbiont Sponge with Antibacterial Activity against Multi Drug Resistant Organism (MDRO) Bacteria from Sea Waters in Sibolga, North Sumatra Indonesia

This research employed the experimental method with descriptive data analysis. It aimed to isolate and identify sponge symbiont bacteria with antibacterial potential against Multi-Drug Resistance Organisms (MDRO) with the 16S rRNA gene marker. Based on the study results, two sponges were obtained, namely Clathrina sp and Agelas sp, each having 11 and 7 isolates of symbiont bacteria, respectively. Based on the antibacterial activity test results, out of 18 isolates of symbiont bacteria, only 4 had antibacterial potential against MDRO (Klebsiella pneumoniae ESBL, Pseudomonas aeruginosa ESBL, or Staphylococcus lugdunensis MRSA) with inhibition zones of 9.3 mm, 8.3 mm, and 8.1 mm, respectively. The results of bacterial identification using the 16S rRNA gene sequence isolate S1I3, S1I9, and A1I3 belong to the Bacillus cereus and Bacillus paramycoides types. This research is expected to provide important information about sponge symbiont bacteria with antibacterial potential against MDRO.

Degradation performance and mechanism of penicillin G in aqueous solution by ionizing radiation

Penicillin G (PG), a frequently detected β-lactam antibiotic in aquatic environment, has attracted extensive concerns because of its extensive application and potential to develop antimicrobial resistance. In this study, gamma radiation was used to degrade PG in water. The main influencing factors, such as initial PG concentration, pH value, absorbed dose, as well as water matrix (inorganic anions and organic matters) were investigated. Results showed that lower initial concentration, acidic condition (pH = 5.57), and higher absorbed dose was conducive to PG degradation. Besides, NO3−, HCO32−, SO42− and HPO42− had negative influence on PG degradation, while Cl− had positive influence. Organic matters had negative influence. Twelve transformation intermediate products (TPs) were identified during PG degradation. Together with the density functional theory (DFT) calculation, four possible pathways of PG degradation were proposed. The results of antibacterial activity test demonstrated that the transformation of PG to TPs eliminated the antibacterial activity. Notably, except some para-modified products of PG, the toxicity of other TPs was reduced PG degradation. Moreover, no potential risk of bacterial mutagenicity during PG degradation was observed. In summary, ionizing radiation has good performance in PG degradation in the real wastewater.

Electrophoretic deposition of graphene oxide reinforced hydroxyapatite on the tantalum substrate for bone implant applications: In vitro corrosion and bio-tribological behavior

Tantalum (Ta) is used in dental implantology due to its high biocompatibility and potential clinical applications. Ta has low bone regeneration and low abrasion resistance , which can lead to inflammatory reactions in biological environments. In the present study, the graphene oxide/hydroxyapatite (GO/HA) composite on the Ta substrate was coated using the electrophoretic deposition technique. The deposition process was conducted at a constant voltage of 30 V at two deposition times of 5 and 10 min. Confocal Raman spectroscopy , Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) confirmed GO in the GO/HA composite coating layer. The roughness of samples increased from 35 ± 3 nm for the GO/HA-coated Ta (at 10 min) to 120 ± 4 nm for the uncoated Ta. After coating Ta with the GO/HA at deposition time of 10 min, nanoindentation curve shifted to the left and maximum depth decreased due to an 18-fold increase in the hardness of coated sample in comparison to the uncoated Ta. The GO/HA composite coating improved the bio-tribological behavior, the resistance to plastic deformation , and fracture toughness of Ta substrate. The stiffness of GO/HA-coated Ta (at 10 min) was 8-fold higher than that of the uncoated Ta. Additionally, the friction coefficient of GO/HA-coated Ta (0.3) was lower than the uncoated Ta (0.43). The GO/HA-coated Ta (at 10 min) showed significantly higher corrosion current density (18.6 ± 1.2 vs 4.97 ± 0.23 μA/cm 2 ) compared to the uncoated Ta. The results of antibacterial activity test indicated that the presence of GO in the GO/HA coating decreased the viability of Escherichia coli and Staphylococcus aureus cells on the surface of Ta. Due to higher surface roughness and wettability of the GO/HA-coated Ta compared to the uncoated Ta, the proliferation and viability of MG-63 cells on the surface of GO/HA-coated Ta significantly increased. Our findings demonstrated that the GO/HA composite coating can enhance the lifetime of Ta based dental and orthopedic implants. • The GO/ HA composite coating was successfully prepared and deposited on the Ta surface during EPD process. • The GO/ HA-coated Ta showed the high corrosion protection and antibacterial activity compare to the uncoated Ta. • The GO/ HA coating improved the bio-tribological behavior, resistance to plastic deformation, and fracture toughness of Ta. • The GO/HA caused an increase in the hydrophilicity and roughness, which enhanced the proliferation of cells on Ta surface. • The GO/HA composite coating on the Ta surface could be a promising candidate for dental and orthopedic implants.

Antibacterial Activity of the Ethanolic Extract of Sembung Rambat (Mikania micrantha Kunth) Leaves Against Bacillus subtilis

Sembung rambat (Mikania micrantha Kunth.) is a weed whose presence is detrimental to agriculture because it can prevent agricultural plants from absorbing nutrients, water and light for photosynthesis. But behind that it turns out that sembung rambat has various benefits, one of which can be used as an antibacterial. The antibacterial ability of sembung rambat leaves has the potential to be used as a basis for making alternative antibiotics. This study was conducted to determine the value of Minimum Inhibitory Concentration (MIC) and antibacterial acitivity of leaves of sembung rambat (M. micrantha Kunth.) Which is most effective in inhibiting the growth of B. subtilis. The sampling method was done by purposive sampling, the extract was made by maceration with ethanol 96% ratio of 1:10. Test for antibacterial activity using the diffusion disc method. The antibacterial activity test results of 96% ethanol extract of sembung rambat leaves, inhibited the test of the value that was the most effective at inhibiting the growth of B. subtilis at 6 % with a diameter of 4.63 mm. From the results obtained, it can be seen that the leaves extract of sembung rambat has the potential to be used as an alternative plant-based antibiotic

Uji Efektivitas Antibakteri Handsanitizer Minyak Atsiri Kalamansi (Citrofortunella Microcarpa) Terhadap pertumbuhan Escherichia coli dan Staphyllococcus Aureus Secara In Vitro

This study aims to determine the antibacterial activity, effective concentration, and the effect of increasing the concentration of Kalamansi orange essential oil in Handsanitizer gel on the inhibition of growth of Eschericia coli and Staphyllococus Aureus bacteria. Testing for antibacterial activity using the agar cakram method. The parameter that is measured is the diameter of the resistivity formed around the disc paper. Antibacterial activity test results were analyzed by descriptive method. The results showed that the concentration of essential oil in the Handsanitizer gel was 1%; 2%; and 4% have provided inhibitory activity for the growth of the tested bacteria. Effective concentration that can inhibit Eschericia coli and Staphyllococus Aureus bacteria at a concentration of 4%. The increase in the concentration of kalamansi orange essential oil showed the wider the diameter of the inhibition zone for bacterial growth. The inhibition that occurs in the Eschericia coli and Staphyllococus Aureus bacteria, proves that the Gel Handsanitizer Preparation Kalamansi orange essential oil contains limonene active compounds which are antibacterial. From the research that has been done, the following conclusions can be drawn: The availability of Kalamansi orange essential oil gel Handsanitizer with a concentration of 1%, 2% and 4% can inhibit the activity of the bacteria Eschericia coli and Staphyllococus Aureus in vitro. With the higher the concentration of kalamansi orange essential oil, the stronger the antibacterial inhibition is produced so that the handsanitizer with a concentration of 4% has the strongest antibacterial power against Eschericia coli and Staphyllococus Aureus bacteria.

FORMULASI DAN UJI AKTIVITAS ANTI BAKTERI GEL PEMBERSIH TANGAN EKSTRAK ETANOL MIDING (STENOCHLAENA PALUSTRIS)

Background Kalimantan is the largest island in Indonesia which has a tropical climate with abundant biological resources. Biodiversity has the potential to be used as medicinal plants. Plant extracts are preferred as antibacterial, one of which is miding plant (Stenochlaena palustris).Aim This study aims to find out the potential of miding plant extracts as an antibacterial against Staphylococcus aureus.Method Miding was extracted using maceration using 70% ethanol solvent. Ethanol miding extraction showed a yield of 3.12% (w / w).Result The extract obtained was carried out by phytochemical test, phytochemical test of miding ethanol extract showed the composition of alkaloid, flavonoid, stereoid, phenolic, and saponin groups.Conclusion Antibacterial activity test results using the well diffusion method. Formula 30%, 20 and 10%. Obtained inhibition zone diameter of 12.50 mm and 20% of 12.00 mm showed greater antibacterial activity compared to a comparison of only 10.02%.

Lactobacilli From Buffalo Milk and Yogurt With Antibacterial Activity Against Gram-Negative Uropathogens

Background: This descriptive cross-sectional study was conducted to determine the antibacterial activity of Lactobacillus strains isolated from buffalo milk and yogurt in Aliabad-e Katul city, Golestan province, north-east of Iran. Methods: Raw milk and yogurt samples were collected and cultured on MRS medium by incubating anaerobically at 37°C for 48 hours. The suspected colonies were identified on the basis of Gram staining, biochemical tests, and carbohydrates fermentation. The antibacterial activity of the cell-free supernatant (CFS) extracted from Lactobacillus strains was determined using the agar well diffusion method against standard strains of Escherichia coli ATCC 11303, Klebsiella pneumoniae ATCC 13883, and Pseudomonas aeruginosa ATCC 15442 as well as gram-negative uropathogens previously isolated from patients with urinary tract infections (UTIs). Three isolates of E. coli (E1, E2, and E3), two isolates of P. aeruginosa (P1 and P2), and two isolates of K. pneumoniae (K1 and K2) were used in this study. Results: A total of 19 Lactobacillus strains were identified as L. plantarum, L. casei, L. acidophilus, and L. helveticus. Based on the results of antibacterial activity test, the isolates had the highest and lowest inhibitory effects on the E. coli and K. pneumoniae isolates, respectively. Among the isolates, only L. casei isolates showed inhibitory activity against K. pneumoniae isolates. Conclusions: In this study, Lactobacilli from buffalo milk and yogurt demonstrated a good inhibitory activity against E. coli as a common cause of urinary tract infection. Therefore, further studies are recommended to elucidate their potential for being used as an alternative to antibiotic therapy.

Aktivitas Antibakteri Nanopartikel Perak Hasil Green Synthesis Menggunakan Ektrak Kulit Buah Kemuning (Murraya Paniculata (L) Jack)

Antibacterial activity using silver nanoparticles synthesized using Murraya Paniculata (L) rinds extract has been carried out. This study aims to determine the antibacterial activity of green synthesized silver nanoparticles using Murraya Paniculata (L) rinds extract. Silver nanoparticles were synthesized by reacting AgNO3 with water extract of the Murraya Paniculata (L) rinds. Green synthesized silver nanoparticles were tested for antibacterial activity using Escherichia coli ATCC 8739. Silver nanoparticles synthesized using Murraya Paniculata (L) rinds extract were characterized using a UV-Vis spectrophotometer to confirm the formation of silver nanoparticles. The UV-Vis spectrophotometer results showed the formation of silver nanoparticles which was indicated by an absorption around 400-450 nm. The antibacterial activity test results of silver nanoparticles showed a strong inhibitory activity.
 Keywords: Green Synthesis, Silver Nanoparticles, Antibacterial, Echerichia Coli

NOVEL COMPOSITE OF SILVER-BACTERIAL CELLULOSE (Ag-BC) FROM SIWALAN SAP (Borassus flabellifer) AND ITS ANTIBACTERIAL ACTIVITY

Recently, a wound healing from natural composite with excellent properties is in a high demand. In this study, a novel composite of bacterial cellulose made from Siwalan sap (Borassus flabellifer) was achieved. Siwalan is a common plant in Java Island of Indonesia and the application is very limited for beverage only. This study aims to determine the effect of the AgNO3/NaBH4 concentration ratio in the development of Ag-BC composites and its antibacterial properties from Siwalan sap. Ag-BC composites were prepared by impregnating the silver solution into the BC matrix through the reduction process with NaBH4. Characterization of Ag-BC composites conducted using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX). Antibacterial assay was performed using disc diffusion method against Salmonella typhimurium (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria. The characterization of Ag-BC composite shows the nanostructure of BC with a length of fiber around 35-60 nm in width. The SEM-EDX micrograph showed that silver particles were impregnated into the BC matrix. Antibacterial activity test results showed that the Ag-BC composite had the ability to inhibit the bacteria S. typhimurium and S. aureus with good inhibition. This result showed the potential application of Ag-BC composite from Siwalan plant as a natural material for medical and pharmaceutical purpose, especially as an antibacterial agent.

PERBANDINGAN AKTIVITAS ANTIBAKTERI INFUSA DAUN JERUK NIPIS (CITRUS AURANTIFOLIA) DAN DAUN JERUK PURUT (CITRUS HYSTRIX) TERHADAP BAKTERI ESCHERICHIA COLI

Plant Lime (Citrus aurantifolia) and Plant Kaffir Lime (Citrus hystrix) including Rutacea family. Part of the plant lemon and lime are used as a drug other than fruit and leaves that can be used as medicine. This study aims to determine the antibacterial activity infuse lime leaves and lime leaves. Examination of the characteristics simplicia macroscopic examination. Phytochemical screening of compounds includes examining alkaloids, flavonoids, tannins and infuse saponin. Ekstrak conducted using distilled water solvent. Test of antibacterial activity against Escherichia coli bacteria carried by the agar diffusion method using a paper disc. Results obtained from macroscopic examination for lime leaves are fresh leaves leaf-shaped single smooth surface and the lower surface of the leaves are light green, the dark green upper surface. If torn, lime leaves produce coarse fibers. The leaves are small with a width of 3-5 cm. For lime leaves are fresh leaves, leaf litter pinnate compound leaves one. Child leaf blade oval to oblong, base rounded or blunt, the blunt end up pointed, 8-15 cm long, 2-6 cm wide, the upper surface colour some what shiny dark green, light green below the surface. Results of phytochemical screening simplicia powder lime leaves and lime leaves that contain a class of alkaloids, flavonoids and tannins. Antibacterial activity test results showed that there were differences between the antibacterial infusion lime leaves and lime leaves kaffir lime leaves which are more effective against the bacteria Escherichia coli compared kaffir lime leaves.

Selenium content, antibacterial, antioxidant and anti-sickling activities of Zanthoxylum gilletii (De Wild) P.G. Waterman (Rutaceae)

Aim: The aim of this study was to identify bioactive compounds, to determine the mineral content and to evaluate the antibacterial, antioxidant and anti-sickling activities of different parts of Zanthoxylum gilletii.Methods: Phytochemical composition was evaluated by general tests as well as chromatographic technics (TLC and HPLC), the mineral micronutrient content was quantified by spectroscopy ICP-OES. The antioxidant activities of the infusions extracts from leaves, stem bark and root bark of Z. gilletii were evaluated using ABTS an DPPH assays, the antibacterial activity against four bacteria strains using the micro-dilution method; and the anti-sickling activity was assessed by the Emmel test.Results: Phytochemical screening revealed the presence of polyphenols such as anthocyanins and flavonoids (stem bark) while stem and root barks contained tannins. Alkaloids were found in the leaves, saponins in leaves, stem and root barks. Leaves and root bark also contained triterpenoids and steroids, while only stem bark contained quinonic derivatives. For phenolic acids and flavonoids, stem and root barks could contain luteolin, chlorogenic acid, caffeic acid and only stem bark could contain rutin. Mineral analysis revealed the presence of macronutrients and micronutrients including calcium, iron, zinc and selenium. All aqueous extracts displayed high ABTS and DPPH radical-scavenging activities at the concentration range of 1–25 ug/mL. The in vitro Emmel test showed that the aqueous extracts of the different parts had anti-sickling properties at the concentration of 10.42 µg/mL, 20.83 µg/mL, 83.30 µg/mL for the stem bark, the leaves and the root bark respectively. The stem bark was the most active extract. The results of antibacterial activity test indicated that the all extracts exhibited the highest activity against Staphyloccocus aureus, Escherichia coli, Enterococcus spp and Pseudomonas aeruginosa. Stem barks showed moderate activity against P. aeruginosa and root barks against S. aureus and Enterococcus spp respectively.Conclusions: The bioactivities of the different parts could be attributed to alkaloids, phenolic compounds and terpenes. Stem bark showed the best antioxidant, antibacterial and anti sickling activities. Z. gilletii contains the phytochemicals that validate its use in Traditional Medicine for the management of sickle cell disease.

UJI AKTIVITAS ANTI BAKTERI EKSTRAK ETANOLIK UMBI AKAR BATU (Coccinia grandis L.Voight) TERHADAP BAKTERI SALMONELLA SP

AbstractSalmonella sp are bacteria that often infect human and animal. This type of bacteria can cause Salmonellosis in human. Previous research has been carried out on secondary metabolites of plants as medicinal ingredients. One of the plants that has potential as a medicine is stone Akar Batu (Coccinia grandis L Voight). This study aims at examining the antibacterial activities of ethanolic extract of stone Akar Batu on Salmonella sp. The extraction method was carried out by maceration and the solvent used was ethanol 96%. Antibacterial activity was conducted using diffusion method. Identification of compound groups of extracts was done by reacting extracts with various chemical reagents. The results showed that stone of Akar Batu powder had a moisture content of 5.99% and a yield of 1.76%. The identification results of ethanolic extract of stone Akar Batu chemical group indicated that the extract contained flavonoids, alkaloids, saponins and tannins. The results of antibacterial activity test have revealed that ethanolic extract of stone Akar Batu can inhibit Salmonella sp.