Antibacterial Activity Of Actinomycetes Research Articles

Isolation, characterization and antibacterial activity of actinomycetes from soils of the Langun-Gobingob Cave Complex in Calbiga, Samar

The discovery of rare Actinomycetes from unexplored areas has proven to be a promising source of new antimicrobials. The Langun-Gobingob cave complex in Calbiga, Samar, Philippines is the second largest cave complex in Asia, yet this is the first reported search for actinomycetes conducted in the area. The main objective of this study was to assess the antibacterial activities of culturable actinomycetes from soil samples from the Langun-Gobingob cave complex. Five actinomycete colonies were isolated in pure culture from soil samples using Starch-Casein Agar medium. The isolates were identified by their morphological characteristics and they were characterized by 16S rRNA sequencing. The sequences were matched to sequences uploaded to the NCBI Basic Local Alignment Search Tool (BLAST). Four of the isolates belonged to the Genus Streptomyces and one belonged to the Genus Nocardia. The antibacterial potential of the isolates was tested against gram-positive and gram-negative test bacteria. One Streptomyces isolate exhibited antibacterial activity against the two gram-positive test bacteria. The limited cave area sampled in this study leaves the cave complex mostly unexplored in the search for antibacterial secondary metabolites.

Antibacterial Activity of Actinomycetes Collected From an Extreme Environment and Coal Mines

Antibacterial Activity of Actinomycetes Collected From an Extreme Environment and Coal Mines

Green Isolation and Characterization of Marine Actinomycetes in Tharangambadi Coastal Area in Nagapattinam District, Tamil Nadu

As a result of the continuous evolution of microbial pathogens towards antibiotic-resistance, therefore development of new and effective antimicrobial compounds. Source of novel antimicrobial metabolites. Further, the antimicrobial metabolites were extracted from the isolate using ethyl acetate solvents and the antimicrobial efficacies were screened by chemical analysis and tested against bacterial pathogens. The metabolites of isolate showed maximum zone of inhibition against all the pathogens viz., Pseudomonas aeruginosa, Escherichia coli, Klebsilla pneumoniae, Bacillus cereus and Staphylococcus aureus as 21 ± 0.12, 20 ± 0.42, 21 ± 1.2, 16 ± 2.01, 15 ± 0.47 respectively. The marine samples which displayed antibacterial activity of actinomycetes may be useful in the clinical conditions where higher secondary metabolites against antibiotic resistant bacteria.

Assessment of the antibacterial activity of actinomycetes isolated from terrestrial soil of King Saud University campus

Assessment of the antibacterial activity of actinomycetes isolated from terrestrial soil of King Saud University campus

Antibacterial activity of actinomycetes against bacterial pathogens of diabetic foot ulcers

Diabetes mellitus is a serious public health problem worldwide. Diabetic foot ulcers (DFU), a major complication in Type 2 diabetes are one of the major causes of morbidity and mortality around the world. To screen various bacterial pathogens present in diabetic foot ulcers and to determine their antibiotic sensitivity to actinomycetes isolated from various fields of Chandragiri, Tirupati, twenty four actinomycetes isolates were isolated and screened by primary and secondary screening methods to determine potent antibiotic producers by using test organisms. Among 24 isolates, 4 were more potent and they showed varied range of antibacterial activity of pathogens, isolated form diabetic foot ulcers. Four isolates were compared with lenezoid antibiotic. Enterococcus was resistant to lenezoid antibiotic but four actinomycetes inhibited the growth of Enterococi.

Diversity and antibacterial activity of actinomycetes from wetland soil

Diversity of soil actinomycetes isolated from various regions of a wetland was analysed. The strains were assigned under 11 genera according to their morphological and biochemical characteristics. They are: Nocardia, Pseudonocardia, Streptomyces, Micromonospora, Rhodococcus, Actinosynnema, Nocardiodes, Kitasatospora, Gordona, Intrasporangium and Streptoalloteichus. Around 55% of the identified strains are Nocardioform actinomycetes. Screening for their antibacterial activities revealed that 96% of the isolated strains showed different degrees of inhibition potential against 12 test pathogenic bacteria (including various serotypes of Salmonella, Vibrio cholerae, Bacillus subtilis and Escherichia coli). Of these about 41% of strains showed antagonism towards Bacillus subtilis. The Nocardioform actinomycetes exhibited antibacterial activity against 9 out of 12 test organisms. The least antibacterial potential was exhibited by Gordona, Rhodococcus, Micromonospora and Kitasatospora. The isolated strains differed among themselves in their ability to decompose proteins and amino acids and also in enzyme production potential.