Antibacterial Activity Of Silver Nanoparticles Research Articles

Physical, optical properties and antibacterial activity of silver nanoparticles: Nanoclusters to nanoparticles formation on glass substrate by in-situ annealing

Physical, optical properties and antibacterial activity of silver nanoparticles: Nanoclusters to nanoparticles formation on glass substrate by in-situ annealing

Anti-Bacterial Action of Silver Nanoparticles Against MDR Bacteria Isolated from Hospital

Hospital-associated infections (HAIs) are considered to be a major source of infections in patients, especially in patients with permanently impaired immunity. There is alarming increase of multi drug resistant (MDR) bacteria and Antibacterial medication resistance has been deemed a serious hazard to public health by the World Health Organisation (WHO). The study aimed to isolate and identify main bacteria caused nosocomial infection, and trying to treatments by using nanoparticles. By measuring the antibacterial activity of the synthesised AgNPs using the agar disc diffusion technique, AgNPs demonstrated antibacterial properties against the Pseudomonas aeruginosa and Staphylococcus aureus MDR.

Characterization and Antibacterial Activity of Silver Nanoparticles Synthesized from Oxya chinensis sinuosa (Grasshopper) Extract.

In this study, silver nanoparticles (AgNPs) were synthesized using a green method from an extract of the edible insect Oxya chinensis sinuosa (O_extract). The formation of AgNPs (O_AgNPs) was confirmed via UV-vis spectroscopy, and their stability was assessed using Turbiscan analysis. The size and morphology of the synthesized particles were characterized using transmission electron microscopy and field-emission scanning electron microscopy. Dynamic light scattering and zeta potential analyses further confirmed the size distribution and dispersion stability of the particles. The average particle size was 111.8 ± 1.5 nm, indicating relatively high stability. The synthesized O_AgNPs were further characterized using X-ray photoelectron spectroscopy (XPS), high-resolution X-ray diffraction (HR-XRD), and Fourier transform infrared (FTIR) spectroscopy. XPS analysis confirmed the chemical composition of the O_AgNP surface, whereas HR-XRD confirmed its crystallinity. FTIR analysis suggested that the O_extract plays a crucial role in the synthesis process. The antibacterial activity of the O_AgNPs was demonstrated using a disk diffusion assay, which revealed effective activity against common foodborne pathogens, including Salmonella Typhimurium, Escherichia coli, Staphylococcus aureus, and Bacillus cereus. O_AgNPs exhibited clear antibacterial activity, with inhibition zones of 15.08 ± 0.45 mm for S. Typhimurium, 15.03 ± 0.15 mm for E. coli, 15.24 ± 0.66 mm for S. aureus, and 13.30 ± 0.16 mm for B. cereus. These findings suggest that the O_AgNPs synthesized from the O_extract have potential for use as antibacterial agents against foodborne bacteria.

ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES OF BRASSICA JUNCEA EXTRACTS AND SILVER NANOPARTICLES: A SYNERGISTIC APPROACH AGAINST ESCHERICHIA COLI

Brassica juncea, a plant known for its anticancer and antioxidant characteristics, is rich in glucosinolates, flavonoids, carotenoids, and phenolic acids. AEBJ exhibited a high concentration of total flavonoids, measuring 201.09 ± 0.232 mg/g in terms of quercetin equivalents. However, it had a relatively lower total phenolic content, measuring 49.43 ± 1.114 mg/g in terms of tannic acid equivalents. The structural characterisation of silver nanoparticles was performed using UV-Visible spectroscopy, followed by the study of these nanoparticles utilising surface plasmon resonance (SPR) measurements. The interaction between silver ions and extracts of Brassica juncea was seen, and the presence of AgNPs was verified through the detection of a visible peak at 425 nm. The Silver Nanoparticle (BJ 1) effectively suppressed the generation of DPPH radicals within the concentration range of 10-100 µg/ml. The linear regression coefficients for this inhibition were calculated to be 0.9869 and 0.9488, respectively. The IC50 values for Silver Nanoparticle (BJ 1) and BHT were 45.413 and 50.173, respectively. Silver Nanoparticle (BJ 1) exhibited a decrease in the conversion of ferricyanide to ferrocyanide as the concentration increased from 50-250 µg/ml. This was accompanied by an increase in the absorption of green light at a wavelength of 700 nm. The investigation into the antibacterial activity of silver nanoparticles (BJ 1) and Brassica juncea extracts against Escherichia coli has demonstrated substantial potential as antimicrobial agents. The findings indicate that both drugs demonstrate inhibitory effects on the growth of E. coli, with the concentration levels playing a substantial influence. This implies a mutually beneficial interaction between the two drugs, which could potentially amplify their efficacy in fighting bacterial infections. Greater doses of Brassica juncea extract exhibited bigger areas of inhibition, so validating its bioactive activities against pathogenic microorganisms. Furthermore, the Silver nanoparticle (BJ 1) had a potent antibacterial impact as a result of their capability to disturb bacterial cell membranes and impede metabolic activities. The combination of these two medicines has the potential to enhance therapy efficacy in clinical settings. Subsequent investigations should delve into the mechanisms underlying their antibacterial properties and evaluate their safety profiles for future medicinal uses. KEYWORDS: Brassica juncea; Silver Nanoparticles; Antioxidant Activity; DPPH; Reducing Power Assay; Antimicrobial Activity; Escherichia coli

Detection of anti-bacterial activity of silver nanoparticles from actinomycetes and testing its efficacy

Detection of anti-bacterial activity of silver nanoparticles from actinomycetes and testing its efficacy

Green Synthesis of Antimicrobial Silver Nanoparticles using Green Tea Extract: The Role of Concentration and pH

ABSTRACTGreen synthesis for silver nanoparticles (AgNPs) is the process of reducing Ag+ ions to Ag0 by using plant bioactive compounds. Green synthesis is safer, environmentally friendly and cost-effective. Plant bioactive compounds from Camellia sinensis L. (green tea) extract containing polyphenols can be used as a reducer in the formation of silver nanoparticles. This study aims to determine the effect of pH (8, 9, and 10), at 1,5mM AgNO3 concentration and 0,75% green tea extract concentration on the physical characteristics and antibacterial activity of silver nanoparticles prepared by green synthesis method. The synthesized results were evaluated by UV-Vis Spectrophotometer, Dynamic Light Scattering (DLS), SEM, and FTIR. The optimal AgNP synthesis process resulted from the AgNO3 concentration of 1.5 mM with 0.75% green tea extract at pH 10 which showed an absorbance value of 4,420 at λ 435,3 nm, with a particle size of 47,1 nm and a PDI of 0,243. The formation of AgNPs was observed by a change in color to brownish yellow. Antibacterial activity of AgNPs using liquid diffusion method. The results obtained AgNPs for Staphylococcus aureus bacteria has had inhibition at a concentration of 0,187mM and Escherichia coli at a concentration of 0,375mM.Keywords: Silver nanoparticles; green synthesis; green tea extract; pH; antibacterial activity

Recent Advances in the Delivery, Mechanism of Action and Antibacterial Activity of Silver Nanoparticles

Study’s Novelty/Excerpt This study comprehensively review the significant advancements in the antimicrobial application of silver nanoparticles (AgNPs), focusing on innovative delivery mechanisms such as nanogels, liposomes, and polymer-based nanoparticles. It highlights the unique physicochemical properties of AgNPs that contribute to their antibacterial efficacy, including their ability to disrupt bacterial cell membranes and inhibit biofilm formation. The review also addresses the critical challenges of cytotoxicity and delivery method refinement, emphasizing the potential of AgNPs in combating antibiotic-resistant bacteria. Full Abstract Nanoparticles,especially silver nanoparticles (AgNPs), have revolutionized various fields like microbiology, biotechnology, pharmacy, and medicine owing to their distinct properties. This research delves into the significant potential of AgNPs in antimicrobial therapy, focusing on recent advancements in their delivery mechanisms, mechanisms of action, and antibacterial efficacy. The effective targeted delivery of AgNPs to specific body sites remains a challenge, leading to innovative approaches in nanotechnology. Nanogels, liposomes, and polymer-based nanoparticles have emerged as promising delivery systems, enhancing the stability, bioavailability, and controlled release of AgNPs. The antimicrobial activity of AgNPs is rooted in their unique physicochemical properties, such as high surface area and reactivity. They disrupt bacterial cell membranes, increasing permeability, causing cell death, and interfering with intracellular components. Additionally, AgNPs have shown potential in inhibiting biofilm formation, a common defense mechanism of bacteria against antibiotics. Despite their promise, addressing issues related to cytotoxicity and refining delivery methods remains imperative. This review comprehensively addresses the challenges associated with the delivery of AgNPs, their cytotoxic effects, and their efficacy against antibiotic-resistant bacteria, highlighting their mechanism of action in bacterial eradication and biofilm inhibition.

Biogenic Synthesis, Characterization, Along with Investigation of Cytotoxicity and Antibacterial Activities of Silver Nanoparticles Using Twigs of Polyalthia sclerophylla

Biogenic Synthesis, Characterization, Along with Investigation of Cytotoxicity and Antibacterial Activities of Silver Nanoparticles Using Twigs of Polyalthia sclerophylla

Green synthesis, characterisation, and antibacterial activity of silver nanoparticles obtained from Salvia officinalis extract

Green synthesis, characterisation, and antibacterial activity of silver nanoparticles obtained from Salvia officinalis extract

Lavandula Stoechas extract; Synthesis of Silver Nanoparticles (Nature-Friendly Green Synthesis Method), Characterization, Antimicrobial Activity and In Silico Molecular Docking Study

Nanoparticles are structures that are applicable to a wide range of fields. The most significant characteristic of these structures is their structural and dimensional diversity, which varies the synthesis process based on the area that will be incorporated into several sections. Although there are other synthetic techniques, the green synthetic technique is the most often used. By employing only natural processes instead of chemicals, this method synthesizes products leaving no by-products. The technique is also highly preferred as it is affordable. In this respect, the green synthesis approach was utilized in this study to investigate the synthesis characteristics, phytochemical content, and evaluate the antibacterial activity of silver nanoparticles from black pepper (Lavandula stoechas), which is commonly used as a sweetener in food and beverages. Furthermore, theoretical investigation was conducted into the glucosamine-6-phosphate synthase inhibitory characteristics of the resveratrol molecule, which was identified as the main constituent based on the HPLC outcome. Characterization tools like FT-IR, UV-Vis, and fluorescence spectroscopy were employed. The phytochemical content was examined by HPLC.

From nature to nanoparticles: Synthesizing silver nanoparticles from Moortalkh plant leaves with potent antibacterial properties

The unique characteristics of nanoparticles, compared to non-nanomaterials, have led to their widespread and increasing use in various fields, especially medicine. Producing nanoparticles using inexpensive, biocompatible, and non-toxic methods is of great importance. Plant-based synthesis of nanoparticles is one desirable method that has gained attention in recent years. In this study, silver nanoparticles were synthesized using aqueous extract of Moortalkh plant leaves at room temperature and pressure. The obtained nanoparticles were evaluated using various tests including UV–Vis spectroscopy, Fourier-transform infrared spectroscopy [FTIR], X-ray diffraction [XRD], field emission scanning electron microscopy [FESEM], and transmission electron microscopy [TEM]. The presence of an absorption peak at the 458 nm region in the UV–vis spectrum of silver nanoparticles indicated the surface plasmon resonance of the nanoparticles, confirming their formation. The XRD analysis results revealed the crystalline nature of the silver nanoparticles, exhibiting face-centered cubic (FCC) structure and an average size of 6.18 nm. FTIR analysis indicated the presence of active molecules from the leaf extract of the Moortalkh plant and their role in the formation and stability of silver nanoparticles. FESEM and TEM analyses showed spherical morphology of nanoparticles with average sizes of 5 to 15 nm and 6.2 nm, respectively. The antibacterial activity of silver nanoparticles against Staphylococcus aureus and Escherichia coli strains was investigated, and the results indicated excellent antibacterial properties of the nanoparticles.

Antibacterial Activity of Silver Nanoparticles Synthesized from Endophytic Streptomyces sp. CR 13 Isolated from the Roots of Ocimum tenuiflorum L.

In this study, Streptomyces sp. CR 13 from the roots of Ocimum tenuiflorum L. was isolated and characterized using biochemical, morphological and genomic approaches. Silver nanoparticles (AgNPs) were synthesized extracellularly using cell-free extract and characterized with UV-vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), EDX, DLS and zeta potential techniques. The maximum peak of UV-visible spectroscopy was observed at 425 nm and the TEM images revealed that the nanoparticles were spherical in shape and ranged in size from 18 to 21 nm. DLS analysis revealed particles with an average diameter of 147.9 nm. The zeta potential analysis of biosynthesized AgNPs showed a peak at -35.2 mV, indicating their high stability. Moreover, AgNPs synthesized by endophytic Streptomyces showed significant antimicrobial activity against Escherichia coli, Klebsiella pneumonia and Stphylococcus aureus.

Synthesis, Characterization, and Antibacterial Activity of Silver Nanoparticles Supported on Bovine Bone Powder Using Heterotheca inuloides: An In Vitro Study

This paper reports on the biosynthesis, characterization, as well as the bactericide and cytotoxic properties of silver nanoparticles supported on bovine bone powder (Ag-NPs/BBP). The silver nanoparticles were obtained through the bioreduction of AgNO3, using an infusion of Heterotheca inuloides leaves and flowers as a reducing agent and bovine bone powder as a support. The ratio of Ag-NPs/bovine bone powder was set as 1:10. The characterization was performed with SEM–EDS, XRD, UV–Vis, and TEM, which showed the formation of nanoparticles with an average size of 22.6 ± 10.8 nm and a quasi-spherical Ag-NPs morphology supported on the BBP surface. The nanocomposite exhibited a band gap of 2.19 eV. The minimal inhibitory concentration and the minimal bactericidal concentration against S. aureus, E. coli, and S. epidermidis were determined for each strain. In addition, the cytotoxic evaluation of the Ag-NPs/BBP on J774.2 mouse macrophage cells was performed. The Ag-NPs/BBP exhibited a bactericide effect on the strains studied, and the cytotoxicity had a dose-dependent behavior on the cells studied. Therefore, it was found that the ecofriendly synthesized Ag-NPs supported on bovine bone powder resulted in an effective bactericidal system against the strains studied, without significant cytotoxicity.

Cytoplasmic Leakage and Protein Leakage Analysis of Ocimum Gratissimum Stem Extract-Mediated Silver Nanoparticles Against Wound Pathogens

ABSTRACT Aim: The current work intends to examine the antibacterial activity of silver nanoparticles (AgNPs) mediated by Ocimum gratissimum stem extract against wound infections. Materials and Methods: To evaluate the membrane damage brought on by AgNPs, analyses of cytoplasmic leakage and protein leakage assays were performed. Results: The outcomes demonstrated that all of the tested bacterial strains were significantly resistant to the AgNPs’ antibacterial activity. AgNPs damaged membranes and caused cellular contents to leak in the target pathogens, according to an examination of protein and cytoplasmic leakage. Conclusion: According to the current investigation, AgNPs mediated by Ocimum gratissimum stem extract may be effective antibacterial agents against microorganisms that cause wounds.

Increased antibacterial activity of silver nanoparticles synthesized from leaf extract of Isodon coetsa

Application of nanotechnology for the synthesis of silver nanoparticles using plant extract can be a promising alternative to traditional chemical methods. Products of biological origin have less side effects, thus are safe for human health besides being cost-effective and pollution-free. Keeping in view this, the current work was designed for the environment- friendly production of silver nanoparticles (AgNPs) utilising Isodon coetsa acetone leaf extract. The effect of some important variables including incubation time, pH, AgNO3 concentration, temperature and volume of plant extract were examined for the optimum production of AgNPs. Synthesized nanoparticles were further characterized using atomic force microscopy (AFM), X-ray diffraction spectroscopy (XRD), and UV-visible absorption spectroscopy. In addition, the antibacterial potential of nanoparticles was also determined by agar-well diffusion method. The optimal conditions for synthesis of AgNPs achieved after 24 h of incubation at pH 9.0, 2mM AgNO3, 60°C temperature, and using 10% I. coetsa leaf extract. X-ray diffraction spectroscopy revealed that the size of the strongest peak for AgNPs was 9.5 nm, while atomic force microscopy confirmed their spherical shape with smooth surface without any pinholes or cracks. AgNPs showed excellent antibacterial action against Gram-positive and Gram-negative bacteria. The current study has contributed to the development of a simple and fast bioprocess for the synthesis of AgNPs of the appropriate size and form which also have increased antibacterial activity in comparison to crude extract. Therefore, the process of biogenically synthesised AgNPs from plants is a straightforward, commercially feasible, and environmentally benign approach that can be used in disease control while utilizing their important role as therapeutic agent for treating various ailments caused by pathogenic bacteria.

Structures, physical properties and antibacterial activity of silver nanoparticles of Lactiplantibacillus plantarum exopolysaccharide

Lactic acid bacteria (LAB) exopolysaccharide (EPS) has good water absorption, high viscosity, good stability, so it was widely used in probiotics fields. In this study, EPS-producing LAB strain Lactiplantibacillus plantarum HDL-03 was isolated and identified. Moreover, the HDL-03 EPS was used as a stabilizer and mixed with AgNO3 to synthesize a novel nanoparticle AgNPs whose structure and properties were explored. The monosaccharide composition and molecular weight indicated that HDL-03 EPS was a heteropolysaccharide composed of mannose and glucose. Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) spectroscopy analysis and methylation results jointly proved it was a heteropolysaccharide containing 1,3-Manp and 1,6-Glcp. The X-Ray diffraction (XRD) results showed that this EPS has an amorphous structure, while the synthesized AgNPs have crystalline properties. The scanning electron microscopy (SEM) and transmission electron microscopy (TEM) results indicated EPS had a smooth and dense sheet structure, while the surface of AgNPs became rougher and large holes appeared after synthesis. Zeta particle size analysis suggested that the particle size of AgNPs increased by 36.63 nm compared to HDL-03 EPS. FT-IR analysis exhibited that the position of the characteristic peaks of AgNPs changed. The OH moving from a wavelength of 3388.49 cm−1 to a wavelength of 3316.79 cm−1 and telescopic vibration peak changed from 1356.07 cm−1 to 1344.22 cm−1. A plate inhibition test revealed the effect of different concentrations of EPS and AgNO3 synthesized AgNPs on the diameter of inhibition circle produced by the indicator bacteria Escherichia coli and Staphylococcus aureus. Furthermore, AgNPs were applied to the indicator bacteria, which the minimum inhibitory concentration (MIC), time-inhibitory curve, and changes in extracellular conductivity, nucleic acids, proteins, ATP, and lactate dehydrogenase (LDH) levels were determined. The AgNPs inhibited the growth of E. coli and S. aureus and exhibited outstanding antimicrobial properties. With the increase of treatment time, the degree of cell membrane damage increased, the permeability enhanced, and the intracellular substances leaked. These results indicate that HDL-03 EPS has good potential for applications in the production of food packaging, antimicrobials, catheters, textiles and coatings.

Optimum Green Synthesis, Characterization, and Antibacterial Activity of Silver Nanoparticles Prepared from an Extract of Aloe fleurentinorum

The synthesis of metal nanoparticles through the use of plant extract is a process that is not only simple but also inexpensive, quick, and favorable to the environment. As a result, it is utilized in a wide variety of fields. When synthesizing silver nanoparticles (AgNPs), several different kinds of plant extracts were utilized. The manufacture of silver nanoparticles was carried out in this study using an environmentally friendly technique. The aqueous extract of the Aloe fleurentinorum plant was utilized as a stabilizing and reducing agent. To determine the optimal conditions for the synthesis of silver nanoparticles, it was necessary to investigate the impact of several parameters on the process. These parameters included the reactant volume ratio, pH values, temperature, and reaction time. To get crystallite and stable silver nanoparticles, an aqueous solution of AgNO3 (0.01M) was added to an aqueous extract of Aloe fleurentinorum plant at a temperature of 60 degrees Celsius and a pH of 8. The mixture was then stirred with a magnetic stirrer for ninety minutes (90 minutes). Using a variety of methods (UV-vis spectrophotometer, FTIR, XRD, SEM, EDX, and XPS), several approaches were utilized to investigate and describe the green-produced AgNPs. Through the use of the SEM method, it was demonstrated that the morphology of AgNPs is tetrahedral. It was determined using X-ray diffraction that the size of crystalline AgNPs was 26.7 nm. AgNPs that have been optimally synthesized have antibacterial properties that are both significant and effective against various bacterial species that have been tested at varying doses.

Study on the Influence of UV Light on Selective Antibacterial Activity of Silver Nanoparticle Synthesized Utilizing Protein/Polypeptide-Rich Aqueous Extract from The Common Walkingstick, Diapheromera femorata.

Common walkingstick (Diapheromera femorata) aqueous extract (CWSAE) can induce the synthesis of useful bionanomaterials. CWSAE is rich in water-soluble organic compounds such as proteins and polypeptides that function as reducing/stabilizing agents for nanoparticle formation from Ag+ ion precursors. The synthesized AgNPs exhibited a moderately uniform size, with the majority falling within the range of 20-80 nm. These AgNPs were UV-treated and tested as antibacterial agents to inhibit the growth of four pathogenic bacteria (Burkholderia cenocepacia K-56, Klebsiella pneumoniae ST258, Pseudomonas aeruginosa PAO1, and Staphylococcus aureus USA300), as well as one common bacterium (Escherichia coli BW25113). The disk diffusion test demonstrated that the UV-treated AgNPs significantly and selectively inhibited the growth of Staphylococcus aureus USA300 and P. aeruginosa, while showing a small effect on the other two species. This suggests the potential application of green-chemically synthesized AgNPs as selective antibacterial agents. Furthermore, we studied the effects of short-term (1-2 min) and long-term (5-30 min) UV treatment on the selective cytotoxicity of the AgNPs and found that the cytotoxicity of the AgNPs could depend on the duration of UV exposure against certain bacteria.

The role of pH-induced tautomerism of polyvinylpyrrolidone on the size, stability, and antioxidant and antibacterial activities of silver nanoparticles synthesized using microwave radiation.

Tautomerism alters the structure and properties of materials, which can be exploited to control their chemical and biological activities. The role of pH-induced tautomerism of polyvinylpyrrolidone (PVP) was determined by measuring the size, stability, and antioxidant and antibacterial properties of microwave synthesized-silver nanoparticles (AgNPs). TEM and XRD analyses confirmed the formation of face-centered cubic silver nanoparticles. PVP stabilized the AgNPs by interaction with the carbonyl or hydroxyl groups depending on the tautomerization under different pH conditions. At pH 4, PVP was stable in the keto tautomer, stabilizing Ag through electron donation of oxygen atoms in the carbonyl group, producing smaller AgNPs with a higher zeta potential. At pH 7 and 9, the enol tautomer PVP stabilized the AgNPs via oxygen atoms in the hydroxyl group, forming large nanoparticles. The keto form of PVP improved the stability and antioxidant and antibacterial properties of AgNPs compared with the enol form. This study also excluded the antioxidant contribution of PVP via hydrogen donation to free radicals. A facile method for controlling the size of AgNPs by adapting the pH-induced tautomerism of PVP that affects their stability and antioxidant and antibacterial activities is thus reported.

Evaluation of antioxidant and antibacterial activities of silver nanoparticles derived from Limonia acidissima L. fruit extract

In the rapidly expanding field of nanoscience and research, AgNPs and their diverse by-products have been acknowledged as having a green approach. Fruit extract of Limonia acidissima L. is capable of bio-reducing AgNO3 and stabilizing AgNPs. AgNPs distinctive absorption peak at 417 nm at pH 8 was visible on the UV-Vis spectrophotometer. The fruit extract contained bioactive chemicals, which may be the cause of the AgNPs bio-capping and stabilizing properties, according to Fourier transform infrared (FT-IR) spectroscopy. Using an X-ray diffractometer (XRD) examination, the AgNPs solid crystalline nature and size of 29.05 nm were investigated. Lastly, AFM and HR-TEM studies verified the AgNPs surface morphology, particularly their size and form. The DPPH assay method was used to measure the AgNPs capacity for radical scavenging. As compared to normal ascorbic acid and fruit extract (control), the AgNPs produced from the fruit extract demonstrated good antioxidant effectiveness. To evaluate the antibacterial activity of the AgNPs sample, it was additionally evaluated against E. coli, S. typhi, V. cholera, and S. aureus. With the greatest zone of inhibition and thus the strongest antibacterial activity, S. aureus (12.03 ± 0.043 mm) was the organism that displayed it. On the other hand, the least active strain was V. cholera (9.09 ± 0.013 mm). The data indicates that bio-fabricated silver nanoparticles (AgNPs) exhibited dose-dependent inhibitory efficacy together with a notable zone of inhibition. Additionally, S. aureus was shown to be more effective than V. cholera. Future health and pharmaceutical fields may benefit from the biocompatible method of separating AgNPs from L. acidissima fruit extract.