Anti-inflammatory Activity Of Essential Oils Research Articles

A novel deep eutectic solvent/switchable-hydrophilicity solvent/H2O system with enhanced CO2 switchability for integrated extraction of phenolics, flavonoids and essential oil from Flos Chrysanthemi Indici flower

Despite the considerable attention received by switchable solvents in natural product extraction and biorefinery, their CO2 switching kinetic properties and extraction ability still present significant challenges. To tackle this issue, the present study has the following innovative endeavors: (1) Proposing a novel approach to enhance the performance of switchable-hydrophilicity solvent (SHS) by incorporating deep eutectic solvent (DES), leading to the successful design of a DES/SHS/H2O system. (2) Developing an innovative high-speed homogenization assisted extraction method using the DES/SHS/H2O system and applying it to the integrated extraction of phenolics, flavonoids, and essential oil from Flos Chrysanthemi Indici. The switching process was characterized using 1H-NMR, the extraction process was optimized employing response surface methodology, the components of extracted essential oils were analyzed by GC-MS, and the cytotoxicity and anti-inflammatory activity of essential oils were determined through MTT and ELISA. Our findings demonstrate that the incorporation of DES can significantly augment the CO2 switching kinetics of SHS, resulting in a 32.7% reduction in switching time and a remarkable decrease of 73.7% residual content within the aqueous phase. The innovative DES/SHS/H2O based extraction method exhibited substantial enhancements in phenolics, flavonoids, and essential oil extraction efficiency compared to conventional approaches, yielding extracts with superior chemical profiles and enhanced anti-inflammatory activity. Therefore, the proposed strategy of enhancing the CO2 switching kinetic properties and extraction ability of SHS by incorporating DES is feasible, providing valuable guidance for the rational design of innovative smart solvent systems. The developed DES/SHS/H2O system, exhibiting improved performance, holds significant application and research prospects.

Potential anti-inflammatory biomarkers from Myrtaceae essential oils revealed by untargeted metabolomics

Many species from Myrtaceae have traditionally been used in traditional medicine as anti-inflammatory, antimicrobial, antidiarrheal, antioxidant and antirheumatic, besides in blood cholesterol reduction. In the present work, the anti-inflammatory activity of essential oils from eighteen Myrtaceae spp. were evaluated according to their ex-vivo anti-inflammatory activity in human blood, and the corresponding biomarkers were determined using untargeted metabolomics data and multivariate data analysis. From these studied species, six displayed anti-inflammatory activity with percentage rates of inhibition of PGE2 release above 70%. Caryophyllene oxide (1), humulene epoxide II (2), β-selinene (3), α-amorphene (4), α-selinene (5), germacrene A (6), β-bisabolene (7), α-muurolene (8), α-humulene (9), β-gurjunene (10), myrcene (11), β-elemene (12), α-cadinol (13), α-copaene (14), E-nerolidol (15) and ledol (16) were annotated as potential anti-inflammatory biomarkers. The results obtained in this study point to essential oils from species of the Myrtaceae family as a rich source of anti-inflammatory agents.

Essential oils from Acronychia pedunculata (L.) Miq. in Vietnam: chemical composition, antimicrobial, cytotoxicity and nitric oxide inhibition activities

The chemical composition, antimicrobial, cytotoxic and anti-inflammatory activities of essential oils from different parts of Acronychia pedunculata, a medicinal plant native to Central Highlands of Vietnam, were investigated. Their chemical constituents were determined using GC-MS. The antimicrobial properties were assessed using a microdilution broth method, while the anti-inflammatory properties were measured based on their capacity to inhibit NO production in macrophage cells. Their cytotoxicity was tested using the sulforhodamine B assay on three human cancer cell lines. The GC-MS analysis revealed the presence mainly of caryophyllene oxide (35.3%) and spathulenol (31.9%) in the stem essential oil, caryophyllene oxide (51.3%), and linalool (21.0%) in the leaf essential oil, and α-pinene (21.4%), (E)-β-ocimene (16.5%), linalool (12.5%), and β-caryophyllene (13.0%) in the fruit essential oil. All three essential oils inhibited the growth of various bacteria and fungi, reduced NO production in LPS-induced RAW264.7 cells, and exhibited cytotoxicity against all three tested cancer cell lines. Notably, the fruit essential oil showed the most potent cytotoxicity among the essential oils, with IC50 values of 5.47 ± 0.21, 7.95 ± 0.35, and 9.22 ± 0.52 g/mL against SK-LU-1, MCF-7, and HepG2 cells, respectively. It also had a strong anti-inflammatory effect that was comparable to that of dexamethasone, with an IC50 value of NO production inhibition of 14.62 ± 0.62 mg/mL. These results suggest the potential of the essential oils from this plant to provide leads for the development of anti-inflammatory and anti-cancer agents.

GC-MS Characterization, in Vitro Antioxidant and Anti-Inflammatory Activities of Essential oil from the Leaves of Litsea balansae Lecomte

Objective: This study aimed to analyze the essential oil obtained from Litsea balansae Lecomte leaves and investigate its potential as an anti-inflammatory and antioxidant agent. Methods: Fresh L balansae leaves were collected in Quangtri, Vietnam, and subjected to hydro-distillation using a Clevenger-type apparatus to extract the essential oil. The essential oil composition was analyzed using gas chromatography–mass spectrometry (GC-MS). Antioxidant capacity was evaluated through the 2,2-Diphenyl-1-picrylhydrazyl assay, while the inhibition of nitric oxide production was assessed in lipopolysaccharide-activated RAW264.7 cells. Results: GC-MS analysis identified 71 compounds, constituting 99.3% of the essential oil. Key constituents included carvacrol (20.8%), caryophyllene oxide (11.0%), cis-β-guaiene (6.4%), khusimone (3.9%), bicycloelemene (3.7%), and spathulenol (3.7%). The essential oil exhibited a moderate antioxidant effect (half maximal inhibitory concentration [IC50] = 208.37 ± 10.67 µg/mL) and significant anti-inflammatory activity (IC50 = 28.09 ± 1.53 µg/mL). Conclusion: The essential oil derived from L balansae leaves showcases potential as an anti-inflammatory and antioxidant agent, as indicated by its chemical composition and biological activities. These findings suggest promising avenues for further research in the development of therapeutic agents from natural sources.

Therapeutic Applications of Essential Oils from Native and Cultivated Ecuadorian Plants: Cutaneous Candidiasis and Dermal Anti-Inflammatory Activity.

Essential oils are a complex mixture of aromatic substances whose pharmacological actions, including antimicrobial, antioxidant, anticancer, and anti-inflammatory activities, have been widely reported. This study aimed to evaluate the anti-Candida and dermal anti-inflammatory activity of essential oils from native and cultivated Ecuadorian plants. Essential oils from Bursera graveolens, Dacryodes peruviana, Mespilodaphne quixos, and Melaleuca armillaris were isolated by hydrodistillation and were characterized physically and chemically. Its tolerance was analyzed by in vitro and in vivo studies. The antifungal activity was studied against Candida albicans, Candida glabrata, and Candida parapsilosis, whereas the anti-inflammatory effect was evaluated by a mouse ear edema model. The main compounds were limonene, α-phellandrene, (E)-methyl cinnamate, and 1,8-cineole, respectively. All essential oils showed high tolerability for skin application, antifungal activity against the three Candida strains, and anti-inflammatory efficacy by decreasing edema and overexpression of pro-inflammatory cytokines. Dacryodes peruviana essential oil showed the highest antifungal activity. On the other hand, Dacryodes peruviana and Melaleuca armillaris showed the greatest anti-inflammatory potential, decreasing edema by 53.3% and 65.25%, respectively, and inhibiting the overexpression of TNF-α, IL-8, IL-17A, and IL-23. The results suggest that these essential oils could be used as alternative therapies in the treatment of both cutaneous candidiasis and dermal inflammation.

Chemical Composition, Analgesic and Anti-Inflammatory Activity of Pelargonium peltatum Essential Oils from Eastern Cape, South Africa.

Pelargonium species are native to South Africa, and they have a long history in medicinal use. This study aimed to extract essential oils from different parts of P. peltatum, determine the chemical composition of the essential oils, and assess the essential oils' biological potential as analgesic and anti-inflammatory agents. The essential oils were obtained by hydro-distilling different parts of P. peltatum, and the essential profile was determined by GC-FID and GC-MS. The analgesic activity of the essential oil was determined by using a tail immersion in hot water method in rats, whereas the anti-inflammatory activity of the essential oils was assessed according to right hind paw oedema induced by egg albumin; the three doses selected for each experiment were 100, 200, and 400 mg/kg. According to the GC-FID and GC-MS analysis, camphene (3.6-33.4%), α-terpineol (4.8-19.1%), α-thujone (1.5-15.6%), piperitone (0.9-12.2%), linalool (1.6-11.7%), myrcene (5.2-10.7%), germacrene D (3.7-10.4%), β-caryophyllene (1.2-9.5%), β-cadinene (3.4-6.7%), and β-bourbonene (4.2-6.2%) were some of the major compounds identified in the oil. P. peltatum essential oils demonstrated analgesic activity by increasing pain latency in hot water; furthermore, in an inflammation test, the essential oil reduced the egg-albumin-induced paw oedema in both the first and second phases. Therefore, the current findings suggest that P. peltatum essential oils have analgesic and anti-inflammatory properties.

Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão) A.C. Smith. from the semi-arid region of Northeastern Brazil

Ethnopharmacological relevanceAmburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro". In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain. However, none of the ethnopharmacological properties has been scientifically evaluated using volatile compounds obtained from its leaves (essential oil). Aim of the studyThis study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis. Material and methodsThe acute toxicity of the essential oil was investigated in mice. The antinociceptive effect was evaluated using the formalin test and, abdominal writhing induced by acetic acid, being investigated the possible mechanisms of action involved in antinociception. The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation. ResultsNo acute toxicity was observed at doses up to 2000 mg/kg; p.o. The antinociceptive effect was statistically equal to morphine. In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP). In peritonitis, a reduction in TNF-α and IL-1β levels and leukocyte migration were observed. The antipyretic effect was statistically superior to dipyrone. The reduction in paw edema was statistically superior to the standard in both models. ConclusionThe results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.

In Vitro Anti-inflammatory Activity of Three Inula Species Essential Oils in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

In the face of the undesirable effects induced by anti-inflammatory drugs, there has been a return, nowadays, to the search for active ingredients based on plants. Herein, for the first time we study the anti-inflammatory activity of essential oils of three species of the genus Inula: Inula viscosa, Inula graveolens and Inula crithmoides in lipopolysaccharide (LPS)-activated macrophages. Essential oils have shown excellent preventive anti-inflammatory potential by causing inhibition of nitric oxide (NO) production in LPSactivated RAW264.7 macrophages with IC50s ranging between 15 and 35 µg mL− 1. On the other hand, the major acidic compounds, more precisely α- and β-costic acids, have been isolated from Inula viscosa and Inula graveolens essential oils and evaluated for their anti-inflammatory effect. These compounds appear to have a moderate preventive inhibitory effect on NO production relative to the significant effect generated by the neutral minority components present in the oils such as borneol, bornyl acetate, (E)-nerolidol, caryophyllene oxide, T-cadinol and eugenol. Therefore, we can deduce that the studied essential oils could be used as anti-inflammatory agents for the treatment of various inflammatory pathologies.

Anti-inflammatory activity of essential oils from Tunisian aromatic and medicinal plants and their major constituents in THP-1 macrophages

In this study, the capacity of eight essential oils (EOs), sage (Salvia officinalis), coriander (Coriandrum sativum), rosemary (Rosmarinus officinalis), black cumin (Nigella sativa), prickly juniper (Juniperus oxycedrus), geranium (Pelargonium graveolens), oregano (Origanum vulgare) and wormwood (Artemisia herba-alba), on the inhibition of NF-κB activation was screened at concentrations up to 0.25 µL/mL using THP-1 human macrophages bearing a NF-κB reporter. This screening selected coriander, geranium, and wormwood EOs as the most active, which later evidenced the ability to decrease over 50 % IL-6, IL-1β, TNF-α and COX-2 mRNA expression in LPS-stimulated THP-1 macrophages. The chemical composition of selected EOs was performed by gas chromatography–mass spectrometry (GC–MS). The two major constituents (>50 % of each EO) were tested at the same concentrations presented in each EO. It was demonstrated that the major compound or the binary mixtures of the two major compounds could explain the anti-inflammatory effects reported for the crude EOs. Additionally, the selected EOs also inhibit>50 % caspase-1 activity. However, this effect could not be attributed to the major components (except for β-citronellol/geranium oil, 40 %/65 % caspase-1 inhibition), suggesting, in addition to potential synergistic effects, the presence of minor compounds with caspase-1 inhibitory activity.These results demonstrated the potential use of the EOs obtained from Tunisian flora as valuable sources of anti-inflammatory agents providing beneficial health effects by reducing the levels of inflammatory mediators involved in the genesis of several diseases.

Screening of essential oil containing plant resources in the Central Highlands region in Vietnam and antimicrobial, anti-inflammatory activities of essential oils of Magnolia citrata Noot. & Chalermglin and Hedyosmum orientale Merr. & Chun

Screening of essential oil containing plant resources in the Central Highlands region in Vietnam and antimicrobial, anti-inflammatory activities of essential oils of <i>Magnolia citrata</i> Noot. &amp; Chalermglin and <i>Hedyosmum orientale</i> Merr. &amp; Chun

Chemical composition and anti-inflammatory activity of essential oils of Melaleuca alternifolia from Senegal

Chemical composition and anti-inflammatory activity of essential oils of Melaleuca alternifolia from Senegal

Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown &lt;i&gt;Daucus&lt;/i&gt; L. Species in Turkey

Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown &lt;i&gt;Daucus&lt;/i&gt; L. Species in Turkey

Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo

Ethnopharmacological relevanceHedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO). Its flower is a traditional Chinese medicine for treating inflammation-related diseases like indigestion, diarrhea, and stomach pain. In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China. Aim of the studyThis research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo. Materials and methodsEO's chemical composition was determined by GC-FID/MS analysis. For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis. Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined. ResultsThe main components of EO were β-pinene (20.2%), α-pinene (9.3%), α-phellandrene (8.3%), 1,8-cineole (7.1%), E-nerolidol (5.4%), limonene (4.4%), borneol (4.1%), and β-caryophyllene (3.7%). For the anti-inflammatory activities in vitro, EO dramatically reduced the LPS-stimulated NO and PGE2 release by suppressing the mRNA and protein expression of iNOS and COX-2. Meanwhile, it remarkably decreased IL-6, TNF-α, and IL-1β production by inhibiting their mRNA levels. Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells. Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue. ConclusionsH. flavum EO exerted significant anti-inflammatory activity in vivo and in vitro, and its mechanism of action is related to the inhibition of MAPK and NF-κB activation. Thus, H. flavum EO could be considered a novel and promising anti-inflammatory agent and possess high potential for utilization in the pharmaceutical field.

GC-MS Characterization, in vitro Antioxidant and Anti-inflammatory Activities of Essential Oil from the Leaves of Stachytarpheta jamaicensis

Abstract. Oxidative stress arising from free radicals have been documented to be a sponsor of inflammation, driving the search for plant-based compounds with antioxidant and anti-inflammatory activities. The aim of this study was to analyze the chemical composition and evaluate the in vitro antioxidant and anti-inflammatory potentials of essential oil obtained from leaves of Stachytarpheta jamaicensis. Essential oil (EO) was extracted using hydro distillation techniques and its components analyzed using gas chromatograph mass spectrometry (GC-MS) techniques. The antioxidant potentials of the EO were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and ferric ion reducing antioxidant power (FRAP) assays. Anti-inflammatory ability was assessed using inhibition of heat and hypotonicity induced hemolysis of the RBC. GC-MS analysis of essential oil of Stachytarpheta jamaicensis showed the presence of limonene (13.85 %), eucalyptol (10.73 %), linalool (5.36 %), cis verbenol (19.54 %), bornyl acetate (12.65 %) and β-phellandrene (5.59 %) as major EO components, representing a total of 67.72 % of the total volatile fraction. The EO showed strong anti-inflammatory properties and a minimal scavenging potential of DPPH and FRAP activity compared to standard agents used. Result obtained from this study indicates that EO of Stachytarpheta jamaicensis is a promising source of antioxidant and anti-inflammatory agents, which can be exploited in pharmaceuticals. Resumen. Está documentado que el estrés oxidativo originado por radicales libres produce respuestas inflamatorias, lo que ha impulsado la búsqueda de compuestos de origen vegetal con actividades antioxidantes y antiinflamatorias. El objetivo de este estudio fue analizar la composición química y evaluar el potencial antioxidante y antiinflamatorio in vitro del aceite esencial obtenido de las hojas de Stachytarpheta jamaicensis. El aceite esencial (EO, por sus siglas en inglés) se extrajo mediante técnicas de hidrodestilación y sus componentes se analizaron mediante técnicas de cromatografía de gases y espectrometría de masas (GC-MS). El potencial antioxidante del aceite esencial se evaluó utilizando ensayos con 2,2-difenil-1-picrilhidrazilo (DPPH) y evaluando el poder reductor de iones férricos (FRAP). La capacidad antiinflamatoria se evaluó mediante la inhibición de la hemólisis de los glóbulos rojos inducida por calor e hipotonia. El análisis GC-MS del aceite esencial de Stachytarpheta jamaicensis mostró la presencia de limoneno (13,85 %), eucaliptol (10,73 %), linalol (5,36 %), cis-verbenol (19,54 %), acetato de bornilo (12,65 %) y β-felandreno (5,59 %) como componentes mayoritarios del extracto, representando un total de 67,72 % de la fracción volátil total. El extracto mostró propiedades antiinflamatorias y un potencial depurador mínimo de la actividad de DPPH y FRAP en comparación con los agentes utilizados como estándares. El resultado obtenido de este estudio indica que el aceite esencial de Stachytarpheta jamaicensis es una fuente prometedora de compuestos antioxidantes y antiinflamatorios, que pueden explotarse en productos farmacéuticos.

Acute toxicity and anti-inflammatory activity of essential oils of Cotula cinerea from southwest Algeria

This work deals with the biological activity of the essential oils of an Algerian plant (Cotula cinerea) which belongs to the Asteraceae family. The study was carried out in the south-west of Algeria. We chose essential oils which are natural substances thanks to their numerous anti-infectious, antioxidant and antibacterial properties, etc. The objective of this work is to study the acute toxicity as well as the evaluation of the anti-inflammatory properties of the essential oils of both organs (leaves and flowers) of Cotula cinerea. As methodological approaches, we used the hydrodistillation technique to obtain the essential oils from leaves and flowers. Thus, the acute toxicity was determined by Lorke's method and the anti-inflammatory activity was evaluated by measuring the plantar oedema of mice induced by carrageenan. The results show that the essential oil extracts of the leaves and flowers are low toxic substances with LD50s of 1131.37 mg/kg and 1264.91 mg/kg respectively. They also show a considerable anti-inflammatory effect at doses not exceeding 300 mg/kg with maximum percentages of 86.16% for leaves and 80.87% for flowers. These results show the efficacy of the essential oils of this plant and their therapeutic richness which can be used in traditional medicine for anti-inflammatory treatment. This study has provided additional knowledge on the biological activity of Cotula cinerea essential oils and has opened perspectives for the research of the active principles contained in this species as well as the application of biotechnology to know the molecular mechanism and valorise this natural wealth.

Anti-Inflammatory Activity of Essential Oil from Zingiber ottensii Valeton in Animal Models.

Zingiber ottensii (ZO) Valeton, a local plant in Northern Thailand, has been widely used in traditional medicine. Many studies using in vitro models reveal its pharmacological activities, including the anti-inflammatory activity of ZO essential oil, extracted from ZO rhizomes. However, the scientific report to confirm its anti-inflammatory activity using animal models is still lacking. The present study aimed to evaluate the anti-inflammatory activity and explore the possible mechanisms of action of ZO essential oil in rats. The results revealed that ZO essential oil significantly reduced the ear edema formation induced by ethyl phenylpropiolate. Pre-treatment with ZO essential oil significantly reduced the carrageenan-induced hind paw edema and the severity of inflammation in paw tissue. In addition, pre-treatment with ZO essential oil exhibited decreased COX-2 and pro-inflammatory cytokine TNF-α expression in paw tissue, as well as PGE2 levels in serum. On this basis, our study suggests that ZO essential oil possesses anti-inflammatory activity in animal models. Its possible mechanisms of action may involve the inhibition of TNF-α expression as well as the inhibition of COX-2 and PGE2 production. These findings provide more crucial data of ZO essential oil that may lead to new natural anti-inflammatory product development in the future.

In vivo Acute Anti-Inflammatory Activity of Essential Oils: A Review.

In recent years, there has been a significant increase in the search for new therapeutic strategies for the treatment of inflammatory diseases. In this sense, natural products emerge as a potential source for the discovery of new drugs, with the research of the pharmacological properties of these products being very important. In addition to its function in plants (insect attraction and repellency), essential oils present pharmacological effects, such as antibacterial, antifungal, antimutagenic, antiviral, antiprotozoal, antioxidant, antidiabetic and anti-inflammatory properties. In this review, we describe the mostly used in vivo acute inflammatory experimental models and the studies showing the in vivo anti-inflammatory activity of essential oils. Essential oil from species from the Apiaceae, Asteraceae, Burseraceae, Boraginaceae, Cupressaceae, Euphorbiaceae, Fabaceae, Lamiaceae, Lauraceae, Myrtaceae, Piperaceae, Poaceae, Rutaceae, Verbenaceae and Zingiberaceae families were described as being anti-inflammatory in vivo. Five models of acute inflammation are commonly used to investigate the anti-inflammatory activity in vivo: ear and paw edema, pleurisy, peritonitis and the subcutaneous air pouch model. In addition to in vivo analysis, ex vivo and in vitro experiments are carried out to study the anti-inflammatory action of essential oils. The most commonly used model was paw edema, especially due to this model being easy to perform. In order to suggest or elucidate the mechanisms involved in the anti-inflammatory effect, many studies measured some inflammatory mediators, such as cytokines, COX-2 expression and the levels of PGE2, and NO, or evaluated the effect of essential oils or their major compounds on inflammation response directly induced by inflammatory mediators.

Chemical composition and anti-inflammatory activity of essential oils from resin of Commiphora species

ABSTRACT. Essential oils (EOs) were prepared by the hydro-distillation technique from the resins of four Commiphora species and analyzed by GC-MS. Major constituents of EOs were a-copaene (22.71%), β-caryophyllene (28.03%) and β-caryophyllene oxide (13.89%) for C. sphaerocarpa; a-pinene (29.1%) for C. africana; hexadecane (14.1%) for C. habessinica and δ-cadinene (31.5%) for C. schimperi. We investigated the anti-inflammatory effects of EOs in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages by measuring nitric oxide (NO). The effect in mRNA or protein level after EO treatment were evaluated by RT-PCR and Western blot analysis, respectively. Among four Commiphora species, C. sphaerocarpa EO demonstrated a significant inhibition of LPS by 27.2±3.6% at 10 μg/mL and 62.3±5.2% at 20 μg/mL. C. sphaerocarpa EO inhibited LPS mediated iNOS over expression in both protein and mRNA level with dose dependent manner. It inhibited phosphorylation of ERK1/2, p38, ATF2. The enhanced anti-inflammatory activity of the EO of the plant was due to HO-1 expression by ROS dependent Nrf2 activation in RAW264.7 cells. These findings indicate C. sphaerocarpa EO inhibits the pro-inflammatory responses by inhibiting MAPK/ATF2, and triggering ROS/Nrf2/HO-1 signaling. Therefore, C. sphaerocarpa EO could have potential for useful therapeutic candidate preventing and treating inflammatory diseases.&#x0D; &#x0D; KEY WORDS: GC-MS, Anti-inflammatory, C. africana, C. habessinica, C. sphaerocarpa, C. schimperi&#x0D; &#x0D; Bull. Chem. Soc. Ethiop. 2022, 36(2), 399-415. &#x0D; DOI: https://dx.doi.org/10.4314/bcse.v36i2.13

Chemical Composition of the Essential Oil and the Solvent Extracts of Curcuma Pseudomontana Rhizomes and in Vitro Evaluation of their Antioxidant and Anti-Inflammatory Activities

The phytoconstituents of the essential oil isolated by hydrodistillation and solvent extracts (PE, BZ, DCM, EtOAc and EtOH) of mature rhizomes of turmeric (Curcuma pseudomontana) were analyzed by GC–MS. The major constituents identified in the oil and the extracts were Benzofuran (6.93%), γ-Curcumene (11.18%), β-Elemenone (22.14%), Germacrone (15.15%), Phenol, 2-Methyl-5-(1,2,2-trimethylcyclopentyl)- (29.58%), Germacrene (8.78%), Italicene (15.30%), Xanthorrhizol (56.52%), Pseudocumenol (24.56%) and Epicurzerenolne (4.95%). Analyzing the anti-inflammatory activity of essential oils (EO), and solvent extracts using HRBC stabilization and antioxidant activity. The results of GC MS analysis showed that while Pseudocumenol the major componenets in the essential oil. Xanthorrhizol is the major component in BZ, DCM and EtOH extracts of the matured rhizomes of C. psedomonata. The results of EO and the solvent extracts from Curcuma possessed good anti-inflammatory and antioxidant activities and hence can be used in natural preservative ingredients in food and/ or pharmaceutical industries.

In Vitro Anti-Inflammatory Activity of Essential Oil and β-Bisabolol Derived from Cotton Gin Trash.

Natural α-bisabolol has been widely used in cosmetics and is sourced mainly from the stems of Candeia trees that have become endangered due to over exploitation. The in vitro anti-inflammatory activity of cotton gin trash (CGT) essential oil and the major terpenoid (β-bisabolol) purified from the oil were investigated against lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages as well as the 3t3 and HS27 fibroblast cell lines. Nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), and interleukin 8 (IL-8) were measured using Greiss reagent, enzyme-linked immunosorbent assay (ELISA), and cytokine bead array (CBA)-flow cytometry. Non-toxic concentrations of CGT oil and β-bisabolol (1.6–50.0 µg/mL) significantly inhibited the production of the inflammatory mediators in a dose-dependent manner. Maximal inhibition by β-bisabolol was 55.5% for NO, 62.3% for PGE2, and 45.3% for TNF-α production in RAW cells. β-Bisabolol induced a level of inhibition similar to an equal concentration of α-bisabolol (50.0 µg/mL), a known anti-inflammatory agent. These results suggest β-bisabolol exerts similar in vitro effects to known topical anti-inflammatory agents and could therefore be exploited for cosmetic and therapeutic uses. This is the first study to report the in vitro anti-inflammatory activity of β-bisabolol in CGT essential oil.