anti-Helicobacter Pylori IgG Antibody Research Articles

Association of Anti Helicobacter Pylori IgG Antibodies in Typhoid Patients in Atbara Teaching Hospital, Sudan

Background: H. pylori infection is still the most common bacterial infection that increases a person's risk of contracting typhoid fever and other gastrointestinal illnesses. Objective: The study's objectives included determining the risk factors for infection as well as the prevalence of H. pylori among typhoid patients at Atbara Teaching Hospital. Materials and Methods: From May to July 2018, a cross-sectional study was carried out in the city of Atbara. Anti-Helicobacter pylori IgG detection using the ELISA technique. Results: Serum samples from 72 cases were tested for anti-H. pylori IgG, and 59 (81.9%) of those samples tested positive. Typhoid fever and the presence of H. pylori were significantly correlated, with P. value (0.000, 0.006,0.000,001,0.02) respectively, corresponding to age, place of residence, tribe, smokers, and coffee drinkers as risk factors. Conclusions: In the research population, a strong correlation between H. pylori and typhoid fever was found, and this correlation grew stronger with age.

Global transcriptomic analysis reveals candidate genes associated with different phosphorus acquisition strategies among soybean varieties.

Soybean adapts to phosphorus-deficient soils through three important phosphorus acquisition strategies, namely altered root conformation, exudation of carboxylic acids, and symbiosis with clumping mycorrhizal fungi. However, the trade-offs and regulatory mechanisms of these three phosphorus acquisition strategies in soybean have not been researched. In this study, we investigated the responses of ten different soybean varieties to low soil phosphorus availability by determining biomass, phosphorus accumulation, root morphology, exudation, and mycorrhizal colonization rate. Furthermore, the molecular regulatory mechanisms underlying root phosphorus acquisition strategies were examined among varieties with different low-phosphorus tolerance using transcriptome sequencing and weighted gene co-expression network analysis. The results showed that two types of phosphorus acquisition strategies-"outsourcing" and "do-it-yourself"-were employed by soybean varieties under low phosphorus availability. The "do-it-yourself" varieties, represented by QD11, Zh30, and Sd, obtained sufficient phosphorus by increasing their root surface area and secreting carboxylic acids. In contrast, the "outsourcing" varieties, represented by Zh301, Zh13, and Hc6, used increased symbiosis with mycorrhizae to obtain phosphorus owing to their large root diameters. Transcriptome analysis showed that the direction of acetyl-CoA metabolism could be the dividing line between the two strategies of soybean selection. ERF1 and WRKY1 may be involved in the regulation of phosphorus acquisition strategies for soybeans grown under low P environments. These findings will enhance our understanding of phosphorus acquisition strategies in soybeans. In addition, they will facilitate the development of breeding strategies that are more flexible to accommodate a variety of production scenarios in agriculture under low phosphorus environments.

Association of serum pepsinogens and gastrin-17 with Helicobacter pylori infection assessed by urea breath test.

BackgroundAssociation of gastric atrophy or cancer with levels of serum pepsinogens, gastrin-17 and anti-Helicobacter pylori IgG antibody have been extensively studied. However, the association of serum pepsinogen and gastrin-17 with H. pylori infection has not been studied in a large population.AimTo investigate the impact of H. pylori infection on serum levels of pepsinogens and gastrin-17.MethodsA total of 354, 972 subjects who underwent health check-ups were included. Serum levels of pepsinogens and gastrin-17 were measured using the enzyme-linked immunosorbent assay. H. pylori infection was detected using 14C-urea breath test (UBT). Multivariable logistic regression analysis was used to investigate the association of serum pepsinogen and gastrin-17 with H. pylori infection.ResultsH. pylori prevalence was 33.18% in this study. The mean levels of pepsinogens and gastrin-17 were higher, while the mean pepsinogen-I/II ratio were lower among H. pylori-positive than -negative subjects. In H. pylori-positive subjects, pepsinogen and gastrin-17 levels correlated positively, whereas the pepsinogen-I/II ratio correlated negatively with UBT values (e.g., the mean serum level of pepsinogen-I in subjects with UBT values in the range of 100-499dpm, 500-1499dpm, and ≥1500dpm was 94.77 ± 38.99, 102.77 ± 43.59, and 111.53 ± 47.47 ng/mL, respectively). Compared with H. pylori-negative subjects, the adjusted odds ratio (aOR) of having pepsinogen-I ≤ 70 ng/mL in the three H. pylori-positive but with different UBT value groups was 0.31 (p<0.001), 0.16 (p<0.001), and 0.08 (p<0.001), respectively; while the aOR of having G-17>5.70 pmol/L was 4.56 (p<0.001), 7.43 (p<0.001), and 7.12 (p<0.001). This suggested that H. pylori-positive subjects with higher UBT values were less likely to have pepsinogen-I ≤70 ng/mL (a serum marker for gastric atrophy), but more likely to have gastrin-17 >5.70 pmol/L (a marker for peptic ulcer).ConclusionsH. pylori-positive subjects with higher UBT values are unlikely to have gastric atrophy, but may have greater risk of severe gastritis or peptic ulcers. Our study suggests that H. pylori-positive patients with high UBT values may benefit the most from H. pylori eradication.

Role of BTLA/HVEM network in development of gastric cancer

The immunopathological mechanism underlying intestinal metaplasia and gastric cancer remain incompletely understood. Regarding the role of B- and T-lymphocyte attenuator (BTLA) / herpesvirus entry mediator (HVEM) in tumorigenesis, this research was conducted to determine the BTLA/HVEM expression in development of gastric cancer. Gastric biopsy and peripheral blood was drawn from 32 non-ulcer dyspepsia (NUD) as control group, 19 intestinal metaplasia (IM), and 63 gastric cancer (GC). BTLA/HVEM expression were analyzed by immunohistochemistry and quantitative real-time polymerase chain reaction. Soluble HVEM (sHVEM) and anti-Helicobacter pylori IgG antibody were assessed by ELISA. Our result showed that BTLA mRNA and protein were significantly increased in advanced stages of gastric cancer. HVEM was higher only at the protein level in the GC group. The sHVEM concentration was also higher in the GC group than in the NUD groups. In addition, we observed H. pylori-positive samples had a lower H-score of HVEM than H. pylori-negative ones. These results suggest that BTLA/HVEM/sHVEM inhibitory pathway is involved in immune regulation and progression of gastric cancer. Therefore, this inhibitory pathway might be a therapeutic target to further immunotherapy of gastric cancer.

Gastric Cancer Screening by Combined Assay for Serum Anti-Helicobacter pylori IgG Antibody and Serum Pepsinogen Levels in the Northwestern Tokyo Area

Gastric Cancer Screening by Combined Assay for Serum Anti-Helicobacter pylori IgG Antibody and Serum Pepsinogen Levels in the Northwestern Tokyo Area

Detection of Anti-Helicopacter pylori IgG Antibody, Anti-GAD antibody, Anti-Beta islets cell in a sample of serum with type-2 Diabetes mellitus patients

We have investigated twenty five patients with type-2 diabetes mellitus aged (35-60) years and fifteen healthy persons as control group to detect Anti-Helicobacter pylori IgG antibody. All studied groups were carried out to measure fasting blood sugar, anti- Glutamic acid decarboxylase (GAD), anti-? islets cells antibody by IFAT, Anti-H. pylori IgG antibody by ELISA technique. There was significant elevation in the concentration of fasting blood sugar than in control group (P &lt; 0.05), the patients had negative results for anti-GAD antibody and anti- ? islets cells antibody, there were significant differences (P &lt; 0.05) of anti-H. pylori IgG antibody in 28 % of patients had type-2 diabetes than control group. This lead to suggestion that type -2 diabetes mellitus patients are more susceptible to H. pylori .

A case of refractory bleeding marginal ulcer of Billroth II gastric resection

A 63-year-old man, undergoing a distal gastric resection with Billroth II anastomosis for duodenal ulcer 20 years ago, was admitted to our hospital with the complaint of tarry stool. Initial treatments included intravenous fluid resuscitation, proton pump inhibitor, and nothing by mouth. Hemoglobin was 6.8g/dl, so patient received a transfusion of packed red blood cells. Emergent upper gastrointestinal endoscopy showed two marginal ulcers at the saddle area of the jejuna site, one was bleeding with exposed vessel, which was treated successfully by endoscopic hemostasis with hemoclips. The next day, the second endoscopy showed the same ulcer rebleeding, so hemoclips were applied again. On the third day after the hospital admission, the third endoscopy showed the other marginal ulcer bleeding with exposed vessel. So the third endoscopic hemostasis procedure with hemoclips was performed on the visible vessels at the base of the other ulcer to stop the bleeding. On the 10th day after the hospital admission he started feeding himself, discharged on the 17th day and received regular follow-up at the outpatient clinical department. Each value of blood gastorin, anti-Helicobacter pylori IgG antibody was in normal-range. CT angiography showed there was no large blood vessel near the marginal ulcer applied hemoclips. In this paper, we reported this difficult to be treated case and review the literature.

Prevalence of cagA in relation to clinical presentation of Helicobacter pylori infection in Egypt

Helicobacter pylori infection, peptic ulcer and gastric cancer are common problems in Egypt. We investigated the prevalence of cagA-positive Helicobacter pylori infections among Egyptian adults in relation to presentation (e.g. dyspepsia vs asymptomatic controls) in Minofyia, Egypt. Patients included men or women seeking care for at least 3 months of upper gastrointestinal symptoms. Helicobacter pylori status was determined by rapid urease test and gastric histopathology in patients and by anti-Helicobacter pylori IgG antibodies in controls. CagA status was determined using an anti-cag A ELISA. 99 Helicobacter pylori infected patients were entered including 90 dyspeptic patients (30 each with gastric cancer, peptic ulcer, and non-ulcer dyspepsia) and 9 non-dyspeptic healthy controls. Age ranged from 27 to 78 y (mean 49.5 y); 50% were men. Anti-cagA antibodies were present in 62.2% of dyspeptic patients compared with 11% of asymptomatic controls (p = 0.004). Anti-cagA antibodies were more prevalent among dyspeptic patients with gastric cancer or peptic ulcer (73.3%) compared to those with non-ulcer dyspepsia (40%) (p = 0.004). The prevalence of cagA in Egypt was related to the clinical presentation of Helicobacter pylori infection being lowest in asymptomatic controls (11.1%) and increasingly prevalent in non-ulcer dyspepsia (40%), peptic ulcer (66.7%), and gastric cancer (89%).

Correlation of Serum Leptin with Circulating Anti-Helico Bacter Pylori IgG Antibodies in End-Stage Renal Failure Patients on Regular Hemodialysis

Leptin is synthesized mainly by the adipose tissue. Leptin also is detected in gastric chief cells. To consider the association of serum leptin with helicobacter pylori- IgG antibodies as parameters of nutritional status, on a group of hemodialysis patients, serum Leptin and serum helicobacter pylori specific IgG (H. Pylori-IgG) antibody titer were measured. Total patients were 36. In all patients there were a significant positive correlation of serum leptin with BMI and a near significant positive correlation of serum leptin with age. In the diabetic HD group a significant inverse correlation of helicobacter pylori IgG antibody titer and serum leptin was seen. This is the first report of the association of serum leptin with pylori-IgG antibodies in hemodialysis patients which may explain nutritional problems that is frequently seen in diabetic hemodialysis patients .More research is needed regarding the clinical consequences of this association.

Seroprevalence of anti chlamydia pnemoniae and anti helicobacter pylori IgG antibodies in coronary artery disease patients

Seroprevalence of anti chlamydia pnemoniae and anti helicobacter pylori IgG antibodies in coronary artery disease patients