Anticaries Agents Research Articles

Tooth-Binding Graphene Quantum Dots Silver Nanocomposites for Prevention of Dental Caries.

The objectives of this study were to develop a tooth-binding graphene quantum dots silver nanocomposites (ALN-GQDs-Ag) and evaluate their antibacterial, mineralising, and discolouring properties for the prevention of dental caries. In this study, ALN-GQDs-Ag were developed by synthesising nano silver (Ag) with graphene quantum dots (GQDs) and functionalised GQDs with alendronate (ALN). ALN-GQDs-Ag were characterised by transmission electron microscopy (TEM), zeta potential analysis, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. The cytotoxicity of ALN-GQDs-Ag against human gingival fibroblasts (HGF-1) and stem cells from human exfoliated deciduous teeth (SHED) was examined using a colorimetric assay with reference to silver nitrate solution. The affinity of ALN-GQDs-Ag for hydroxyapatite particles was investigated using inductively coupled plasma spectroscopy (ICP). The antibacterial properties of ALN-GQDs-Ag against Streptococcus mutans were evaluated by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony-forming units counting (CFUs). The mineralisation properties of ALN-GQDs-Ag on human dentine were assessed using micro-computed tomography (micro-CT), scanning electron microscopy (SEM), and Fourier transform infrared in a biochemical cycling model. The discolouring properties of ALN-GQDs-Ag on artificial dentine caries were determined using spectrophotometry. TEM, Zeta potential, XPS, FTIR, and Raman spectroscopy confirmed the synthesis of stable spherical ALN-GQD-Ag nanocomposites with a 10.3 ± 5.5 nm diameter. The colorimetric assay demonstrated that ALN-GQDs-Ag were less cytotoxic than silver nitrate to HGF-1 and SHED (p<0.001). ICP showed that ALN-GQDs-Ag were bound to hydroxyapatite. SEM, CLSM, and CFUs showed that ALN-GQDs-Ag was bactericidal and inhibited biofilm growth of Streptococcus mutans. Micro-CT, SEM, and FTIR showed that ALN-GQDs-Ag repressed dentine demineralisation under a cariogenic challenge. Spectrophotometry revealed no significant discolouration of dentine caries in the ALN-GQDs-Ag. This study developed a biocompatible and tooth-binding ALN-GQDs-Ag with promising antibacterial, mineralising, and non-discolouring properties. ALN-GQDs-Ag could be a novel anti-caries agent for preventing dentine caries if translated for clinical use.

Surface Prereacted Glass Ionomer Varnish as a Multifaceted Anticaries Agent: Investigating its Inhibitory Effects on Demineralization and Biofilm Formation on Primary Tooth Enamel

Surface Prereacted Glass Ionomer Varnish as a Multifaceted Anticaries Agent: Investigating its Inhibitory Effects on Demineralization and Biofilm Formation on Primary Tooth Enamel

Association of household chemicals use with cognitive function among Chinese older adults

BackgroundThe paucity of empirical evidence supporting a correlation between the utilization of household chemicals and cognitive decline in Chinese older adults. MethodsThe data utilized for this study originated from the Chinese Longitudinal Healthy Longevity Survey (CLHLS 2018). Using regression models to investigate the relationship between exposure to household chemicals and cognitive decline, and evaluate the impact of different fields on cognitive function. ResultsThe use of household chemicals was associated with a decline in cognitive function (anti-caries agent, OR = 1.68, P = 0.040; air freshener, OR = 2.48, P = 0.002; disinfectant, OR = 1.40, P = 0.033). The more frequent the use of household chemicals, the worse the cognitive function (Model1: OR = 2.54, P = 0.024; Model2: OR = 3.23, P = 0.006; Model3: OR = 3.59, P = 0.003). ConclusionThe study has uncovered a correlation between the utilization of household chemicals and cognitive decline in individuals aged 65 years and over in China.

Developing a novel antibacterial copper tetraamine fluoride

ObjectiveTo develop a novel and biocompatible copper tetraamine fluoride (CTF) with antibacterial and nondiscolouring properties. MethodThis study used copper fluoride and ammonia solution to develop CTF solution. The CTF was characterized by X-ray photoelectron spectroscopy (XPS). Cytotoxicity was evaluated by stem cells from human exfoliated deciduous teeth (SHED) and human gingival fibroblasts (HGF-1). The fluoride concentration was determined using ion-selective electrode. The alkalinity was measured by a pH electrode. The human dentine blocks were treated with CTF and then incubated with Streptococcus mutans to evaluate the antimicrobial and discolouring effects. The silver diamine fluoride (SDF) was employed as the positive control, and water was the negative control. The colony-forming units (CFUs) and confocal laser scanning microscopy (CLSM) were used to examine the kinetics and viability of the biofilm. The discolouring property on dentine was assessed by spectrophotometry. One-way analysis of variance with the Bonferroni post hoc test was performed to assess and compare the data. ResultsXPS confirmed synthesis of CTF solution. The half-maximal inhibitory concentration of CTF on SHED and HGF-1 was 195±16 ppm and 137±11 ppm. The fluoride concentration was 121,000±5,000 ppm. The pH value was 9. Log10 CFU of the CTF, SDF and water group were 5.0 ± 0.2, 4.9 ± 0.1 and 7.4 ± 0.1 (p < 0.001, CTF, SDF<Water). CLSM showed that the dead-to-live ratio of the CTF, SDF and water group were 0.8 ± 0.2, 0.8 ± 0.2, and 0.3 ± 0.1 (p < 0.001, CTF, SDF>Water). Spectrophotometry showed that the ΔE of the CTF, SDF and water group were 5 ± 2, 6 ± 3 and 45±2 (p < 0.001, CTF, Water<SDF). ConclusionThis study developed an alkaline 58% CTF solution, which is biocompatible, antibacterial and non-discolouring. Clinical significanceIf CTF is successfully translated into clinical care, CTF can be a simple and affordable anti-caries agent for clinicians to prevent dental caries.

Mapping the Dental Applications of Nanosilver Fluoride: A Narrative Review.

The main objective of this paper is to discuss the current progress in the field of dentistry and highlight the aspects regarding silver nanoparticle incorporation, emphasizing the properties, applications, and advantages of nanosilver fluoride (NSF) that it brings to dentistry. An extensive electronic scientific search was conducted on published articles in various databases, such as Medline (PubMed), CENTRAL (Cochrane), Scopus, and Web of Science, using the search terms AgNPs, nano dentistry, caries prevention, and oral health. Further brief communications, randomized controlled trials (RCT), in vitro research, and animal studies written in English were also considered. Case reports, editorial reviews, and opinion letters were excluded from the first phase of our research. Pertaining to various kinds of literature reviews in journals, around 345 articles were retrieved. After screening, about 28 articles met all the selection criteria, focusing on NSF for the contemporary management of dental caries, emphasizing microinvasive therapeutic methods that can successfully halt the progression of caries at the initial level and minimize the loss of sound tooth structure. Due to its exceptional properties and wide range of clinical applications, AgNPs incorporated in fluoride may be employed as an effective, affordable, and improved anticaries agent that brings about superior enhancements in the fields of orthodontics, restorative dentistry, and pediatric and preventive dentistry. Thimmaiah C, Thomas NA, Baskaradoss JK, et al. Mapping the Dental Applications of Nanosilver Fluoride: A Narrative Review. Int J Clin Pediatr Dent 2024;17(7):833-837.

Solubility and hydrolysis of ammonium hexafluorosilicates as potential pharmaceuticals (a review)

This review analyzes and systemizes results from publications on the solubility and hydrolysis of ammonium hexafluorosilicates (AHFS). These inorganic compounds have a variety of technological applications and have been actively researched as potential anti-caries agents in recent years. The characteristics of AHFS solubility were examined in water, methanol, ethanol (96%), and dimethyl sulfoxide. Existing approaches were discussed to interpret the main trends in changes of salts solubility depending on cation structure. Interionic H-bonds were noted to have a non-trivial negative impact on the water solubility of AHFS. The hydrophobic effect of H-bonds also needed to be considered when searching for new pharmaceutical substances among AHFS. A high, often close to quantitative, degree of hydrolysis of AHFS was found in diluted aqueous solution, resulting in an effective release of fluoride ions with caries-preventive properties.

Knowledge, Attitudes, and Practices towards Silver Diamine Fluoride among Dentists in Vietnam.

Silver diamine fluoride (SDF) is a topical solution used for managing dental caries. The mixed-methods study consists of a quantitative study (questionnaire survey) and a qualitative study (in-depth interview) to explore the knowledge, attitudes, and practices towards SDF among dentists in Vietnam. A descriptive approach and a thematic approach were performed to analyze data, respectively. The questionnaire survey invited 436 licensed dentists registered for the national annual dental meeting and 226 dentists participated (response rate: 52%). Among them, 174 (77%, 174/226) dentists knew SDF, and 69 (40%, 69/174) dentists used SDF for caries management. Most of them considered SDF therapy as non-invasive (84%, 146/174) and simple (80%, 140/174). However, most of them expressed concern that SDF could discolor the tooth (74%, 128/174). Their most preferred teeth for SDF therapy were primary posterior teeth (92%, 160/174). The in-depth interview consulted 16 dentists to reach data saturation. They learned about SDF from outside curriculum resources as an effective anti-caries agent. They understood the advantages (simple, non-invasive, timesaving) and disadvantages (tooth discoloring, ammonia odor) of SDF. They used SDF to arrest caries in uncooperative children in the clinic and people living in rural areas in outreach services. Most dentists in Vietnam are supportive of SDF therapy, and they know its advantages and disadvantages for caries management. The results addressed the aim of the study to investigate Vietnamese dentists' perspectives towards SDF.

Formulation and evaluation of toothpaste for sensitivity

Toothpastes are complex mixtures of abrasives and surfactants; anticaries agents, such as fluoride; tartar control ingredients; pH buffers; humectants (to prevent dry-out and increase the pleasant mouth feel); and binders, to provide consistency an shape. Binders keep the solid phase properly suspended in the liquid phase to prevent to the separation of the liquid phase out of the toothpaste. The dental paste preparations of clove oil tooth paste are designed using different bases for treatment of gingivitis and properties and other dental plaque. During our physicochemical evaluation studies, all the formulations were found to have PH, good tube extrudability, good spread ability and viscosity characteristics. In the current context of oral dental care, the use of toothpaste containing natural ingredients are more widely accepted in public belief than chemical-based formulations for safety and efficacy in reducing tooth decay, and preventing other dental problems that this generation is prone to. It's usually a toothpaste-used product used by all people. Toothpaste is usually used to clean tooth enamel and mouth It is also used to solve many email problems. Many dentists recommend applying toothpaste to treat diseases such as sensitivity, chronic gingivitis, etc toothpastes are used in the treatment of gum disease, bad breath, dry mouth, periodontal damage, tartar, and dental caries. To develop a stable and functionally effective toothpaste, eliminating all synthetic additives that are usually included in such Formulation is an important task. Herbal toothpastes have received a lot of attention for reducing gingivitis.Studies show mixed results about the effectiveness of these toothpastes.

Postoperative instructions for silver diamine fluoride therapy: A scoping review of current evidence and practice

ObjectivesSilver diamine fluoride (SDF) has gained popularity in dentistry as an anti-hypersensitive and anti-caries agent. This study aims to review the postoperative instructions for SDF therapy and examine the evidence supporting these instructions in treating dentine hypersensitivity and caries control. Data and sourcesTwo independent reviewers searched three electronic databases (EMBASE, PubMed, and Web of Science) to identify clinical studies that used SDF to treat dentine hypersensitivity and dental caries. Additionally, they searched Google Chrome for information from manufacturers, the department of health, national dental organisations, authoritative dental organisations, and universities regarding SDF therapy for these conditions. Data on postoperative instructions were extracted, and their impact on the treatment of dentine hypersensitivity and caries control was investigated. Study selectionThis review included 74 clinical studies, 11 sets of instructions from SDF manufacturers, and six recommendations from national dental organisations, departments of health and universities. Amongst the included records, 50 clinical studies, two manufacturers, and one department of health mentioned postoperative instructions for SDF therapy. The recommendations varied, from suggesting no specific instructions, immediate rinsing, refraining from eating or drinking for 30 to 60 min, and even avoiding brushing until the next day after SDF therapy. Notably, no clinical studies reported the effects of these postoperative instructions on the SDF treatment of dentine hypersensitivity or dental caries. ConclusionsThis scoping review highlights the presence of inconsistent postoperative instructions for SDF therapy in the treatment of dentine hypersensitivity and caries control. Furthermore, there is a lack of evidence supporting the effectiveness of these instructions. Clinical significanceNo evidence supports the postoperative instructions for SDF therapy for managing dentine hypersensitivity and dental caries. Studies are necessary to provide guidance for clinicians using SDF in their clinical practice.

Antibacterial Activity of Euphorbia tirucalli against Lactobacillus acidophilus: An In Vitro Study

Dental caries is a common oral disease that occurs due to excessive interaction between tooth structure, bacterial biofilm, and glucose. Lactobacillus acidophilus is the main cause of dentin dental caries, which can be prevented using anticaries agents. However, current anticaries agents may cause some side effects. Therefore, there is a need for natural anticaries agents with minimal side effects. Euphorbia tirucalli contains flavonoids, tannins, and saponins that contribute to its antibacterial activity. This study aimed to determine the antibacterial activity of E. tirucalli against L. acidophilus bacterial growth. This was an in-vitro study with a post-test-only control group design, divided into three groups. The positive control group received 0.2% chlorhexidine digluconate, the negative control group received aquadest, and the intervention groups received extracts with concentrations of 10 mg/mL, 20 mg/mL, 30 mg/mL, 40 mg/mL, and 100 mg/mL. Minimum Inhibitory Concentration (MIC) was examined post-treatment using the agar dilution method. The differences in colony growth were analyzed using Cramer's V and Fisher's Exact Test, with p&lt;0.05 considered significant. E. tirucalli extracts showed antibacterial effects against L. acidophilus with a concentration of 40 mg/mL as the MIC. Extract groups with concentrations of 40 mg/mL and 100 mg/mL showed significant differences in L. acidophilus growth compared to the negative control group (p&lt;0.001). The study concluded that E. tirucalli extract has antibacterial activity against the growth of L. acidophilus, with MIC of 40 mg/mL.

Cellulose Nanocrystals Show Anti-Adherent and Anti-Biofilm Properties against Oral Microorganisms.

Cellulose nanocrystals (CNCs) are cellulose-derived nanomaterials that can be easily obtained, e.g., from vegetable waste produced by circular economies. They show promising antimicrobial activity and an absence of side effects and toxicity. This study investigated the ability of CNCs to reduce microbial adherence and biofilm formation using in vitro microbiological models reproducing the oral environment. Microbial adherence by microbial strains of oral interest, Streptococcus mutans and Candida albicans, was evaluated on the surfaces of salivary pellicle-coated enamel disks in the presence of different aqueous solutions of CNCs. The anti-biofilm activity of the same CNC solutions was tested against S. mutans and an oral microcosm model based on mixed plaque inoculum using a continuous-flow bioreactor. Results showed the excellent anti-adherent activity of the CNCs against the tested strains from the lowest concentration tested (0.032 wt. %, p < 0.001). Such activity was significantly higher against S. mutans than against C. albicans (p < 0.01), suggesting a selective anti-adherent activity against pathogenic strains. At the same time, there was a minimal, albeit significant, anti-biofilm activity (0.5 and 4 wt. % CNC solution for S. mutans and oral microcosm, respectively, p = 0.01). This makes CNCs particularly interesting as anticaries agents, encouraging their use in the oral field.

The Synergism of Silver and Fluoride: A New Arsenal in Caries Management

AbstractTopical anticaries agents are non‐invasive methods to control and prevent dental caries. SDF is a fluoride‐based agent added to the arsenal of caries prevention tools. It has got a lot of attention since its introduction in the 1970s because of its ability to prevent and arrest caries, as well as its excellent antibacterial and anti‐plaque action. SDF reduces acid production by microorganisms, decreases the Streptococcus mutans count, biofilm formation, increases enamel resistance, and promotes dentinal tubule obliteration when applied to the dental surface. SDF also assists in the remineralization of dentin. A biannual application of 38% SDF has been effective at preventing dentin demineralization. This “silver bullet” can be seen as an alternative for caries prevention in special health care needs children which is minimally invasive and safe. Along with the advantages, there are some disadvantages, and work has been carried out to explore the use of silver nanoparticles (SNP) as anticaries agents to shield the teeth from aesthetic damage caused by Silver diamine fluoride. SNPs are identified to have the potential to prevent and inhibit dental caries while not discoloring demineralized dental enamel. SNPs have a greater surface area for interaction with microorganisms than other particles, making them more effective antimicrobial agents. Fluoride combined with SNP solution has a synergistic effect that promotes enamel remineralization while also acting as a bactericidal agent against cariogenic microorganisms. This review aims to bring out the various advantages and disadvantages related to the use of SDF and SNPs to aid in future research.

The Role of Anti-Caries Agent Sodium Fluoride Application in Rampant Caries Patients

The Role of Anti-Caries Agent Sodium Fluoride Application in Rampant Caries Patients

The antimicrobial activity of theobromine against cariogenic microbes: an in vitro pilot study

ObjectiveThis pilot study aimed to compare the antimicrobial effect of theobromine, sodium fluoride, and a theobromine-sodium fluoride combination against the following caries-associated bacteria: Streptococcus mutans and Actinomyces naeslundii.MethodologyAntimicrobial susceptibility was tested via the broth microdilution method, with suspensions cultured on each microbe’s respective selective media. Shapiro–Wilk’s was completed and all the data showed normality (p > 0.05), and One-way ANOVA was applied to infer the significant differences in the viable counts between the groups.ResultsAll experimental conditions for both S. mutans and A. naeslundii groups resulted in a significantly lower bacterial abundance in comparison to the control medium, without any active antimicrobial agent (p < 0.001). There was no significant difference in viable count between the theobromine, fluoride, or combination groups against either microbe (p > 0.05).ConclusionTheobromine’s antimicrobial activity against S. mutans and A. naeslundii was found similar to that of fluoride, whether used independently or in combination. Further testing of theobromine is necessary to assess its role as an alternative anticaries agent.

Evaluation of Formulated Herbal Toothpaste Containing Aloe Vera Gel and Extracted Calcium Carbonate from The Shell of Buccinum Undatum (Whelk Snail)

Prevalence of tooth decay in individuals attributes to bacterial buildup in the mouth and inconsistent effectiveness of certain commercial toothpastes and this has prompted the formulation of herbal toothpaste. The formulation is crafted with diverse bases to address gingivitis, dental caries and plaque, hence the toothpaste consist of intricate blends of abrasives, surfactants, anticaries agents, tartar control elements, pH buffers, humectants and binders ensuring consistency, shape and product stability. The herbal toothpaste formulation was prepared, incorporating aloe vera and calcium carbonate extract from the shell of Buccinum undatum. The focus of the composition aims to investigate the antimicrobial potential of the actives (aloe vera and calcium carbonate) in the toothpaste using cultures of S. aureus, P. aeruginosa and K.pneumoniae (prevalent micro organisms in the oral cavity) and comparing with a reference standard ( Colgate toothpaste). Utilizing the hot liquid phase technology, the toothpaste was formulated, and then evaluated. The Physicochemical evaluation studies reveals the formulation to have such characteristics as pH-10, good foamability and abrasiveness, spreadability of 5.5 gcms-¹, viscosity-4600 cP and appreciable stability. Anti microbial studies indicated notable zone of inhibition for the selected organisms as: K.pneumoniae – 5.5 mm and P. aeruginosa – 5.0 mm with no effect on S.aureus. The formulated herbal toothpaste consisting of aloe vera and extracted calcium carbonate was observed to display appreciable antimicrobial and abrasive properties hence further study is required to enhance its acceptable application for potential oral effects, and consideration for long term public dental health use.

In vitro evaluation of 2-(chloroalkyloxy)naphthalene-1, 4-dione derivatives as anticaries agents

In vitro evaluation of 2-(chloroalkyloxy)naphthalene-1, 4-dione derivatives as anticaries agents

Effect of treatment with phosphate, casein phosphopeptide and fluoride on the remineralization: in vitro study.

This study aimed to evaluate in vitro the effect protocols and anticaries agents containing casein amorphous calcium fluoride phosphopeptide-phosphate (CPP-ACPF, MI Paste Plus), sodium trimetaphosphate (TMP) and fluoride (F), in remineralization of caries lesions. Bovine enamel blocks with initial caries lesions were divided into groups (n = 12): 1) Toothpaste without F-TMP-MI Plus (Placebo); 2) Toothpaste 1100 ppm F (1100F), 3) 1100F + MI Paste Plus (1100F-MI Paste Plus), 4) Toothpaste with 1100F + Neutral gel with 4,500 ppm F + 5%TMP (1100F + Gel TMP) and 5) Toothpaste with 1100F + Neutral gel with 9,000 ppm F (1100F + Gel F). For the 4 and 5 groups the gel was applied only once for 1 minute, initially to the study. For the 3 group, after treatment with 1100F, MI Paste Plus was applied 2x/day for 3 minute. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); profile and depth of the subsuperficial lesion (PLM); concentrations of F, calcium (Ca) and phosphorus (P) in enamel was determined. The data were analyzed by ANOVA (1-criterion) and Student-Newman-Keuls test (p < 0.001). Treatment with 1100F alone led to ~ 28% higher remineralization when compared to treatment with 1100F associated with MI Paste Plus (p < 0.001). The 1100F and 1100F + Gel F groups showed similar values for %SHR (p = 0.150). 1100F + Gel TMP treatment also remineralized the enamel surface by ~ 30% and 20% when compared to the 1100F + Gel F and 1100F groups (p < 0.001). The lower lesion depth (ΔKHN) was observed for the 1100F + Gel TMP group (p < 0.001), where it was 54% and 44% lower in comparison to the 1100F and 1100F + Gel F groups (p < 0.001). Polarized light microscopy photomicrographs showed subsurface lesions in all groups, but these lesions were present to a lower extent in the 1100F + Gel TMP group (p < 0.001). Treatment with 1100F + Gel TMP promoted an increase in the concentration of Ca in the enamel by ~ 57% and ~ 26% when compared to the 1100F and 1100F + MI Paste Plus groups (p < 0.001), respectively. There were no significant differences between the 1100F, 1100F + MI Paste Plus and 1100F + Gel F groups (p > 0.001). Similar values of P in the enamel were observed in the 1100F, 1100F + MI Paste Plus and 1100F + Gel F groups (p > 0.001), except for the 1100F + Gel TMP group, which presented a high concentration (p < 0.001). We conclude that the 1100F+TMP gel treatment/protocol led to a significant increased remineralization when compared to the other treatments/protocols and may be a promising strategy for patients with early caries lesions.

Assessing the phenolic content and antimicrobial properties of Cinnamomum porrectum, Dioscorea bulbifera, and Tristaniopsis whiteana extracts for anticaries agent

In this study, we have assessed the phenolic content and antimicrobial properties of three plants (Cinnamomum porrectum, Dioscorea bulbifera, and Tristaniopsis whiteana) against S. mutans and S. sobrinus, bacteria known to contribute to dental caries. The study focused on investigating the antimicrobial properties using the nutrient broth dilution method. Phytochemical screeing was performed using quantitative analysis, which revealed the total polyphenol and total tannin content in the studied plants. The results of the antimicrobial activity test prove that extracts from T. whiteana exhibibited strong inhibition activity against S. sobrinus and S. mutans. The inhibition rates were measured at 76.3% and 72.18%, repectively. These findings suggest that extracts from T. whiteana have the potential to be effective antimicrobial agent against these bacteria. Based on these results, the study concluded that the tested plant extracts have proven antimicrobial efficacy, supporting their potential use as natural antimicrobial. The study highlighted T. whiteana as the promising candidate among tree plants in this study, by demonstrating strong potential as an anticaries agent against microorganisms responsible for dental caries. Furthermore, research and exploration of these plant extracts may supply valuable insight into their mechanisms of action and their potential application in the field of medicine.

Reinforced dentin remineralization via a novel dual-affinity peptide

ObjectivesIn light of the constantly flowing saliva, anti-caries remineralization agents are inclined to be taken away. Owing to their limited residence time, the remineralization effect is not as desirable as expected. Hence, our study aimed to synthesize a novel peptide (DGP) with high affinity to both collagen fibrils and hydroxyapatite, and investigated its dentin remineralization efficacy in vitro and anti-caries capability in vivo. MethodsDGP was synthesized through Fmoc solid-phase reaction. The binding ability and interaction mechanism of DGP to demineralized dentin were investigated. Dentin specimens were demineralized, then treated with DGP and deionized water respectively. The specimens were incubated in artificial saliva and in-vitro remineralization effectiveness was analyzed after 14 days. The rat caries model was established to further scrutinize the in-vivo efficacy of caries prevention. ResultsDGP possesses an enhanced adhesion force of 12.29 ± 1.12 nN to demineralized dentin. The favorable adsorption capacity is ascribed to the stable hydrogen bonds between S2P-101 and ASP-100 of DGP and GLY33 and PRO-16 of collagen fibers. Abundant mineral deposits and remarkable tubule occlusion were observed in the DGP group. DGP-treated dentin obtained notable microhardness recovery and higher mineral content after a 14-day remineralization regimen. DGP also demonstrated potent caries prevention in vivo, with substantially fewer carious lesions and significantly lower Keyes scoring. SignificanceDGP proves to possess a high affinity to demineralized dentin regardless of saliva flowing, thus enhancing remineralization potency significantly in vitro and in vivo, potential for dental caries prevention and combatting initial dentin caries clinically.

Modern Trends in Oral Biology Research

Modern Trends in Oral Biology Research