Ant Nest Density Research Articles

Reaction of Wood Ants to a Large-Scale European Spruce Bark Beetle Outbreak in Temperate Forests

In the Białowieża Forest, Norway spruce is the preferred host tree species for wood ants, both in coniferous and mixed stands; thus, spruce mortality as a consequence of a continuous spruce bark beetle outbreak in the Białowieża Forest since 2012 could have severe consequences for wood ant colonies, as well as their vitality and distribution. The main aim of this study was to assess whether the bark beetle outbreak had any effects on wood ant nest density and abundance and whether we could find any factors affecting the dead spruce distribution around ant nests. A re-inventory of active and abandoned wood ant nests in our study area was conducted from April to July 2022, using the same procedure as the previous inventory performed in 2016. The wood ant nest density was 0.11 per ha and remained practically the same relative to that determined in 2016. Our results indicate that, despite the importance of spruce for wood ants, in situations when only part of the spruce trees die, natural forest disturbances such as bark beetle outbreaks can actually have positive effects because more light can reach the forest floor, thereby promoting the establishment of new nests.

Impact of Large-Scale Fire and Habitat Type on Ant Nest Density and Species Abundance in Biebrza National Park, Poland

Fire can have negative effects on the ant community by reducing species abundance through direct mortality, changes in resource availability, or foraging activity. Fire can also have positive effects, especially for opportunistic species preferring open or disturbed habitats. We assessed the direct effects of a large-scale fire on ant communities in open habitats (grassland and Carex) and moist forested peatland (birch and alder) sites in Biebrza National Park, testing three hypotheses: (i) the large-scale fire had more significant effects on ant nest density in forests than in open habitats, (ii) the post-fire ant diversity changes within sites are stronger in forests than open habitats, and (iii) ant species preferring disturbed habitats are favoured by the fire event. The fire had negative effects on ant nest density only in the Carex and grassland sites but not in the birch and alder sites, suggesting that fire had a stronger impact in open habitats than in forests. Temporal post-fire ant diversity changes within sites were stronger in forests than in open habitats. We observed higher beta diversity changes between the first and second year of the study in the burned forest sites due to colonisation, indicating a greater fire impact on species community composition followed by a higher recolonisation rate. Ant species preferring disturbed habitats were favoured by the fire. The seed-eating ant species Tetramorium caespitum, a thermophilous and opportunistic species, dominated the burned grassland site. This contrasts with other species, e.g., Lasius alienus, for which nest density decreased after fire, underlining the importance of food resource availability as a major driver of community changes after fire. Our study also underlines the importance of periodic biodiversity monitoring in conservation areas for assessing the recovery of the original status after disturbances and revealing possible habitat changes endangering the survival of local biotic communities.

Increased cover of native and exotic plants on the rims of harvester ant (Hymenoptera: Formicidae) nests under grazing and drought.

Harvester ants create habitats along nest rims, which some plants use as refugia. These refugia can enhance ecosystem stability to disturbances like drought and grazing, but their potential role in invasion ecology is not yet tested. Here we examine the effects of drought and grazing on nest-rim refugia of 2 harvester ant species: Pogonomyrmex occidentals and P. rugosus. We selected 4 rangeland sites with high harvester ant nest densities in northern Arizona, USA, with pre-existing grazing exclosures adjacent to heavily grazed habitat. Our objective was to determine whether nest refugia were used by native or exotic plant species for each site and scenario of drought and grazing. We measured vegetation cover on nest surfaces, on nest rims, and at 3 distances (3, 5, and 10 m) from nests. At each site, we sampled 2 treatments (grazed/excluded) during 2 seasons (drought/monsoon). We found that nest rims increased vegetation cover compared with background levels at all sites and in almost all scenarios of treatment and season, indicating that nest rims provide important refugia for plants from drought and cattle grazing. In some cases, plants enhanced on nest rims were native grasses such as blue gramma (Bouteloua gracilis) or forbs such as sunflowers (Helianthus petiolaris). However, nest rims at all sites enhanced exotic species, particularly Russian thistle (Salsola tragus), purslane (Portulaca oleracea), and bull thistle (Cirsium vulgare). These results suggest that harvester ants play important roles in invasion ecology and restoration. We discuss potential mechanisms for why certain plant species use nest-rim refugia and how harvester ant nests contribute to plant community dynamics.

Impact of invasive little fire ants Wasmannia auropunctata on rainforest soil fauna: implications for conservation of the endangered flightless kagu of New Caledonia

The little fire ant, Wasmannia auropunctata, is an invasive species known to negatively affect many native species in its introduced range. Here, we studied the effect of little fire ant presence on the biomass of soil fauna in rainforest of New Caledonia to assess if little fire ants have an impact on an endangered endemic flightless bird, the kagu, Rhynochetos jubatus. The average biomass of soil fauna (except for millipedes) was lower in 53 sample plots with little fire ants than in 597 sample plots without little fire ants and the biomass decreased with increasing density of little fire ant nests. Millipedes on the contrary seemed to be positively impacted as their average biomass was higher in areas with fire ants than in areas without, but even millipede biomass decreased with increasing nest density of fire ants. Kagu density decreased with increasing area of rainforest invaded by little fire ants, suggesting, given their observed negative impact on soil fauna biomass, that they may limit food resources for kagu. Little fire ants might therefore transform rainforest into a less suitable habitat for the kagu by reducing the biomass of their prey and thus have a negative impact for kagu conservation.

Disentangling the effects of host resources, local, and landscape variables on the occurrence pattern of the dusky large blue butterfly (Phengaris nausithous) in upland grasslands

Determining the effects of local and landscape drivers on endangered species and predicting potential suitable habitats for their persistence is crucial for effective conservation management. Here, we applied a multi-scale approach to disentangle the effects of host resources, local, and landscape variables on the occurrence pattern of Phengaris (= Maculinea) nausithous in semi-natural upland grasslands. Our approach comprised the assessment of host parameters (plant cover, density, height, flower heads density, ant nest density, ant colony size), local grassland management (pasture, meadow), site conditions (area, shape, terrain attributes), and landscape variables (landscape composition, connectivity). We used ensemble of small models based on bivariate generalized linear models for explaining and predicting the butterfly occurrence pattern. Bivariate models revealed that host ant nest density, plant cover and height, local grassland management type (pasture), slope and eastness, landscape forest cover and grassland connectivity had a positive effect on the occurrence of P. nausithous (average explained deviance 20.5%). Host ant density, host plant cover, and local grassland management were the most influential factors on the ensemble predictions. The presence of P. nausithous in upland grasslands is not only determined by host resources, but also by local and landscape factors. Such factors proved to be relevant for identifying and predicting suitable grassland sites for this endangered species. Consequently, we recommend that conservation actions should include a landscape perspective to promote connectivity by facilitating coherent grazing networks enabling dispersal between semi-natural upland grasslands and thus species persistence.

Predation of top predators: cane toad consumption of bullet ants in a Panamanian lowland wet forest

Abstract:Despite a clear consensus about the major roles that predators play in shaping ecological communities, descriptive studies of interactions between ecologically important top predator species are underreported. Native cane toad consumption of predatory bullet ant nests was verified through multiple, independent observations taken on Pipeline Road, Panama. Cane toad predation led to the extirpation of 42% of the nests within a 1.05 km2area that is characterized as a late-successional wet forest. This predation pressure could be significant given the high rate of predation events and low bullet ant nest density observed here (0.12 nests ha−1). Implications of this interaction for the local bullet ant population, possible top-down effects and trophic cascades resulting from this top predator interaction are discussed.

Harvester ant nest distribution depends on soil disturbance regime

Nest densities of harvester ants (Messor barbarus) are high in rain-fed cereal fields in north-eastern Spain where the ants remove large quantities of seeds, contributing to reductions in weed populations. The distribution of harvester ant nests within a field can influence the effectiveness of ants as weed seed predators because areas with low ant nest density have lower weed seed removal rates. Tillage can disturb or even kill ant colonies and may be an important factor explaining the distribution of nests within fields. During the summers of 2011–2013, the number of nests in a 50 × 50 m area in 4 tilled and 3 no-till fields were counted. Tilled fields were disturbed twice a year, in November before cereal seeding and in July, after cereal harvest, whereas no-till fields had no soil disturbance. Ant nests were evenly spaced in no-till fields whereas nests were randomly distributed in tilled fields. Our results provide evidence that no-till in cereal fields promotes a more even distribution of M. barbarus nests, which should result in higher and more regular levels of weed seed predation across the field.

Phengaris (Maculinea) teleius butterflies select host plants close to Myrmica ants for oviposition, but P. nausithous do not

AbstractMany lycaenid butterfly species have interactions with ants, with 12% obligatorily depending on two sequential sources of larval food, namely host plants and host ants. When host plants are abundant but the density of host ant nests is relatively low, most host plants have no host ant nest in their vicinity and are thus unsuitable for larval survival. Obligatorily myrmecophilous female butterflies, whose caterpillars feed on ant larvae, would have a comparative advantage if they deposit eggs on host plants in the proximity of a host ant nest. However, this ant‐mediated oviposition has been hotly debated. In an open‐air insectory experiment, we tested whether oviposition is ant‐mediated or not for two obligatory myrmecophilous butterfly species, Phengaris (Maculinea) nausithous Bergsträsser and Phengaris teleius Bergsträsser (Lepidoptera: Lycaenidae). Female butterflies could select host plants close to either no ant nest or a nest of one of the two Myrmica species (Hymenoptera: Formicidae) that are thought to be their host ant. Our results support ant‐mediated oviposition in P. teleius, but also indicate that there is no preference for either of the Myrmica species. More eggs were deposited and more caterpillars were found on long flowerheads close to Myrmica ant nests than on those without nests. Our findings suggest that ant presence is more important than flowerhead phenology for females of P. teleius. In contrast, P. nausithous females were not attracted by ants but preferred long flowerheads with a low percentage of green coloration. With these findings, this study contributes to better understanding of the habitat requirements of two highly specialized butterfly species.

Efectos del sitio y la condición de agostadero en la densidad de hormigueros de escamoles (Liometopum apiculatum Mayr)

El efecto de la condición de agostadero en la densidad de hormigueros de la hormiga de escamoles (Liometopum apiculatum Mayr) se evaluó en tres sitios diferentes (favorable, regular y desfavorable), en un espacio dividido en dos patrones contrastantes de aprovechamiento y régimen de tenencia comunal (ejido) y privada (rancho) en Charcas, San Luis Potosí, México. La vegetación se midió utilizando el método de cuadrantes centrados en un punto, espaciados sobre transectos; en cada cuadrante se midió el individuo más próximo al punto correspondiente a cada estrato de la vegetación y se registró la distancia al hormiguero más cercano (si existía). Las especies se agruparon de acuerdo con su valor forrajero (deseables, menos deseables e indeseables) y, mediante estimaciones de densidad y biomasa volumétrica, los grupos se contrastaron con un análisis tabular y estadístico descriptivo. La superficie del suelo se evaluó con el método de líneas de intercepción en la proporción de cobertura basal viva y muerta (mantillo), suelo desnudo, rocas, caminos de hormigas y heces. La densidad más alta de los hormigueros (14·ha-1) se registró en el sitio favorable del rancho (mejor condición de agostadero). Con base en la información registrada se encontraron diferencias en la densidad de hormigueros entre sitios de agostaderos contiguos, lo cual a su vez es alterado severamente por la condición de los mismos.

Transition of Vegetation States Positively Affects Harvester Ants in the Great Basin, United States

Invasions by non-native plants can alter ecosystems such that new ecological states are reached, but less is known about how these transitions influence animal populations. Sagebrush (Artemisia tridentata) ecosystems are experiencing state changes because of fire and invasion by exotic annual grasses. Our goal was to study the effects of these state changes on the Owyhee and western harvester ants (Pogonomyrmex salinus Olsen and P. occidentalis Cresson, respectively). We sampled 358 1-ha plots across the northern Great Basin, which captured unburned and burned conditions across 1−≥31 years postfire. Our results indicated an immediate and consistent change in vegetation states from shrubland to grassland between 1 and 31 years postfire. Harvester ant occupancy was unrelated to time since fire, whereas we observed a positive effect of fire on nest density. Similarly, we discovered that fire and invasion by exotic annuals were weak predictors of harvester ant occupancy but strong predictors of nest density. Occupancy of harvester ants was more likely in areas with finer-textured soils, low precipitation, abundant native forbs, and low shrub cover. Nest density was higher in arid locations that recently burned and exhibited abundant exotic annual and perennial (exotic and native) grasses. Finally, we discovered that burned areas that received postfire restoration had minimal influence on harvester ant occupancy or nest density compared with burned and untreated areas. These results suggest that fire-induced state changes from native shrublands to grasslands dominated by non-native grasses have a positive effect on density of harvester ants (but not occupancy), and that postfire restoration does not appear to positively or negatively affect harvester ants. Although wildfire and invasion by exotic annual grasses may negatively affect other species, harvester ants may indeed be one of the few winners among a myriad of losers linked to vegetation state changes within sagebrush ecosystems.

Analysing spatial correlation of weeds and harvester ants in cereal fields using point processes

The interaction between the spatial distribution of weed richness and weed cover and the spatial location of harvester ant nets was investigated in cereal fields. The understanding of such interdependencies can be relevant to understand weed population dynamics in dryland cereal fields and may enhance management strategies for weed control. We used spatial statistical tools derived from point process theory. In particular, we compared the two spatial configurations by assuming two different point patterns. We did so by replacing the random weed fields by a related point pattern and comparing it with the point pattern of harvester ants. Our results suggest that areas with a high density of ant nests are, in this case study, in areas with low weed richness and that large nests have a greater impact than small nests. Considering that only one field was analysed, preserving and enhancing regular ant nest distributions, especially of large nests, might have an impact on depleting weeds and consequently enhancing weed control.

Distribution characteristics of ant mounds and correlating factors across different succession stages of tropical forests in Xishuangbanna

In order to understand the distribution characteristics of ant mounds and influencing factors in tropical forests, we surveyed ant species, density, coverage and spatial distribution patterns of ant mounds in three successional stages of tropical forests in Xishuangbanna and analyzed correlations between these variables with soil physico-chemical properties. We found that (1) ant species richness, density and coverage of ant mounds in the three successional stages of tropical forests were ranked as: Musa acuminata community > Mallotus paniculatus community > Mellettia leptobotrya community; (2) successional forest type significantly impacted ant species richness and density of ant mounds but had no significant effects on ant mound coverage; (3) ant species richness and nest density was significantly and positively correlated with total soil organic carbon and soil hydrolysis nitrogen, but negatively correlated with soil bulk density and soil moisture content, besides, there were no significant correlations between soil physico-chemical properties and mound coverage. The spatial patterns of ant mounds indicated a random distribution. We conclude that the successional vegetation types and soil properties may exert a crucial effect on ant species richness and settlement density in secondary tropical forests of Xishuangbanna.

Effect of patch size on seed removal by harvester ants

SummaryThe harvester ant Messor barbarus can be responsible for high weed seed losses in dry land cereals in Spain. Because weeds occur in patches, harvester ants have to be able to find and exploit patches. However, seed patches can differ in size and may, therefore, differ in the probability of being discovered and exploited. Here, 90 patches varying in size from 0.25 to 9 m2 were created in three 50 × 50 m subareas in a cereal field. Oat seeds were sown as weed seed surrogates in the patches at 2000 seeds m−2. After 24 h, those remaining were collected and the exploitation rate (the percentage of seeds removed per patch discovered by ants) was estimated. Harvester ant nests and the location of the seed patches were georeferenced and used to estimate distances between them. The patch encounter rate (the proportion of patches discovered by the ants) decreased slightly, but significantly, with decreasing patch size, though not the exploitation rate, which was lowest in the smallest patches (78–94%) and highest in the largest (99–100%). Seed patches that were not found or partially exploited were mostly located in subareas with a lower ant nest density or a longer distance away from the nearest nest than seed patches that were fully exploited. The results of this study indicate that the interaction between the spatial distribution of ant nests and the patchy distribution of seeds can create opportunities for seeds to be subjected to lower levels of predation.

Correlation of the Nest Density and the Number of Workers in Bait Traps for Fire Ants (Solenopsis invicta) in Southern China

The relationship between Solenopsis invicta nest density and the number of fire ant workers in bait traps and percentages of traps capturing ants were investigated in the waste land of Wuchuan, Guangdong, South China. The results showed that fire ant nest density is positively correlated with the number of workers captured in traps, and could be described by N=60.53LnD +348.0D+421.1. The workers exceeded 200 and 300 in bait traps while the density of fire ant nests was over 0.023 and 0.084 ind./m2, respectively. The percentages of traps capturing ants were also positively correlated with fire ant nest density and fit by Pe=1/(1+e0.9694-309.85D). When the nest density was over 0.018 ind./m2, over 99% of traps captured fire ant workers. N=8.8796e0.0346Pe was the fitting line for worker amount and trap percentage. The workers per trap were about 50, 100, and 200 when the trap percentages were 50%, 70% and 90%, respectively.

Altered nitrogen and precipitation along urban gradients affect harvester ants and seed sources

We investigated the effects of nitrogen deposition and precipitation on Messor pergandei (Mayr) harvester ants and plants to identify alterations in the desert food web in California. We measured ant colony attributes and shrub fruit densities, as well as nitrogen and carbon concentrations and stable isotopes, at 18 sites along a nitrogen deposition gradient. Ant nest density increased from low to high deposition sites; however, ant nest mound size and the density of abandoned nests decreased as deposition increased. Nest mound size was positively correlated with the size and age of the colony; therefore, these results suggest that colonization has been more frequent with increased inter-colony competition in areas of high deposition. Nitrogen and carbon isotope values of perennial plant leaves and seeds, annual plant seeds, and ants were significantly enriched in the heavy isotopes from low to high nitrogen deposition regions, indicating the possibility of plants assimilating different sources of both elements, including anthropogenically-produced compounds. Plant carbon isotope discrimination also differed with the decrease in precipitation across the gradient. Considering that deserts are limited by both nitrogen and precipitation, our results suggest that altered nitrogen inputs in conjunction with precipitation may result in cascading effects through trophic levels and drive arid ecosystem change.

An update of the world survey of myrmecochorous dispersal distances

We update the global assessment of seed dispersal by ants and test the hypothesis that the body size of seed‐dispersing ant species varies with latitude in the same way as dispersal distance. We compiled all published data about seed dispersal distance by myrmecochory through March, 2011. We then broke the data down by vegetation type, geography and taxonomy. We also compiled data on body size (body length) of the seed‐dispersing ant species from the studies consulted. Based on 7889 observations, the mean dispersal distance was 1.99 m, although the curve has a long tail extending to 180 m. Considering the mean dispersal distance by ant species and study as independent data, the mean dispersal distance was 2.24 ± 7.19 m (n = 183). Shorter distances are associated with smaller ant species, while the tail of the dispersal curve is due to larger ant species. The mean dispersal distance of myrmecochorous seeds dispersed by ants decreased with increasing latitude, but there was no significant relationship between the body size of dispersing ant species and latitude (i.e. myrmecochorous seed‐dispersing ant species do not follow Bergmann's rule). In 1998 we made three predictions: 1) the dispersal distances of the Southern Hemisphere will decrease with as more data from mesophyllous vegetation are obtained; 2) assuming that ant nest density is higher at lower latitudes, the differences in distances between hemispheres would probably decrease with more data; and 3) numerical differences between dispersal distances in mesophyllous and sclerophyllous vegetation zones would increase with more data. The results obtained since 1998 support the only the third prediction. The dispersal distances in mesophyllous vegetation zones are shorter than in the sclerophyllous vegetation zones, and the difference between 1998 have increased. The differences in dispersal distances between hemispheres are consistent with the avoidance of parent–offspring competition (escape hypothesis).

Estimating density of ant nests using distance sampling

The quantification of ant nest densities is a use- ful but challenging task given the group's high abundance and diversity of nesting sites. We present a new application of a distance-sampling method which follows standard distance analytical procedures, but introduces a sampling innovation that is particularly useful for ants; instead of having an observer look for ants we let ants find a bait station and measure the distances covered between nest and station. We test this method by estimating the density of epigaeic ant nests in an Amazon tropical forest site near Manaus, Brazil. We distributed 220 baits of canned sardine mixed with cassava flour among 10, 210-m long transects in old-growth upland forest. Forty-five minutes after baiting, we followed the ants' trails and measured the linear distance between the bait and each nest's entrance. We then used the freely available program DISTANCE to estimate the num- ber of nests per unit area while accounting for the effect of distance on the probability that a colony will find a bait. There were found 38 species nesting in 287 different colo- nies, with an estimated 2.66 nests/m 2 . This estimate fell within the 95 % confidence bounds of nest density predicted for a similar number of species based on a literature survey of ant species richness and nest density. Our sampling solution, however, takes less than 30 % of the time used by conventional sampling approaches for a similar area, with the advantage that it produces not only a point estimate but also a quantification of uncertainty about density.

The habitat vegetational structure and the success of the blue alcon, Maculinea alcon (Denis & Schiffermüller)

In the present work, we report the first reference for blue alcon, Maculinea alcon (Denis & Schiffermüller) (Lepidoptera, Lycaenidae), in Portugal. This butterfly species maintains a complex relationship with its host plant marsh gentian (Gentiana pneumonanthe L., Gentianaceae) and with Myrmica ants in its habitat in Northern Portugal. This study involved a multivariate analysis of the relationship between M. alcon and selected habitat variables aimed at proposing a suitable methodology for the conservation of this species. Three habitats were distinguished in terms of their floristic combinations: density of ferns (more humid and deep soils, Habitat A), size of grasses (left wetlands, Habitat B) and shrubs (productive wetland, Habitat C). In these habitats, several G. pneumonanthe and M. alcon parameters were recorded as well as the number of host ant nests. Taller plants and higher number of Maculinea eggs were observed in the habitats with higher values ofshrubs and grasses cover (Habitats B and C), whereas the higher number of plants was stimulated by higher values of shrub cover (Habitat C). In contrast, more humid soils and low cattle pressures reduce the density of plants and ant nests and, consequently, the presence of Maculinea. However, the existence of ant nests is decreased under herbaceous layers. These results suggest that the right balance between the number of gentians and Myrmica nests will ensure the survival of theM.alcon butterfly. Thus, the management of the ecosystems where these species occur, i.e. the Erica tetralix hygroscopic communities, requires the maintenance of the balance between the grass and the shrub layers in order to ensure the existence of Myrmica nests and, consequently, the survival of M. alcon.

Harvester ant ( Messor barbarus (L.)) density as related to soil properties, topography and management in semi-arid cereals

Harvester ants are important weed seed predators in semi-arid cereal fields. Densities of harvester ants vary considerably across fields and so do concomitant weed seed predation rates, however, causes for variability are largely unknown. Understanding the factors that affect ant density may help to identify areas where high natural weed seed control is likely and management factors that could help to enhance ant densities where they are low. Messor barbarus ant density was approximated by estimates of nest density and nest size, and these were correlated to different edaphic, topographic and crop management factors in 34 no-till and 6 minimally tilled cereal fields. The number of years since conversion to no-till explained 12.6% of variability in ant nest density. No other variable could explain nest densities. M. barbarus ant and nest densities increased with increasing number of years of no-till, reached a peak at 11–12 years, after which densities decreased again. Overcompensation by a large cohort of newly established queens could provide an explanation for this observation. Other factors not included in this study, in particular food (seeds) availability, may play an additional role in determining ant density.

Density and spatial pattern of nests in sub-Mediterranean ground-dwelling ant communities (Hymenoptera: Formicidae)

The density and spatial pattern of ant nests were examined in two sub-Mediterranean habitats of the Balaton Uplands in Hungary. The aim of this study was to examine whether regular spatial arrangement, the most common dispersion pattern among ants that mostly indicates competition, is a characteristic feature of these communities. In both habitats, the nests of Aphaenogaster subterranea and Lasius emarginatus had the highest density, followed by the nests of Prenolepis nitens and Temnothorax crassispinus, which were also frequent. The intraspecific spacing pattern of nests was random or aggregated in most cases, and when the species identity of the nearest neighbour was disregarded, the nests of all the individual species showed significant aggregation. The nearest interspecific inter-nest distances proved to be significantly shorter than the nearest intraspecific inter-nest distances both in the case of the entire community and the majority of individual species, except for the polydomous L. emarginatus. On the basis of the results it seems that neither intra-, nor interspecific competition may be an important process in the formation of nest spacing patterns in these habitats.