Annual Forage Legumes Research Articles

Pod-shattering characteristic differences between shattering-resistant and shattering-susceptible common vetch accessions are associated with lignin biosynthesis

The common vetch (Vicia sativa L.) is a self-pollinated annual forage legume that is widely distributed worldwide. It has wide adaptability and high nutritional value and is commonly used as an important protein source for livestock feed. However, pod shattering seriously limits the yield of common vetch. To clarify the mechanism of pod shattering in common vetch, the pod walls of three shattering-resistant (SR) accessions (B65, B135, and B392) and three shattering-susceptible (SS) accessions (L33, L170, and L461) were selected for transcriptome sequencing. A total of 17190 differentially expressed genes (DEGs) were identified in the pod wall of B135 and L461 common vetch at 5, 10, 15, 20, and 25 days after anthesis. KEGG analysis showed that “phenylpropanoid biosynthesis” was the most significantly enriched pathway, and 40 structural genes associated with lignin biosynthesis were identified and differentially expressed in B135 and L461 common vetch. We analysed the DEGs in the pod wall of three SR and three SS accessions at 15 days after anthesis, and most of the DEGs were consistent with the significant enrichment pathways identified in B135 and L461 common vetch. The total lignin content of SR accessions was significantly lower than the SS accessions. The present study lays a foundation for understanding the molecular regulatory mechanism of pod shattering related to lignin biosynthesis in common vetch and provides reference functional genes for breeders to further cultivate shattering-resistant common vetch varieties.

Replenishment of the VIR perennial forage crops collection with samples from a collecting mission in Tambov and Ryazan provinces in 2018

The purpose of the collecting mission was to replenish the collection of perennial forage crops with crop wild relatives (CWR) of interest for breeding purposes, as well as for the subsequent preservation of forage CWR diversity in natural populations. The mission was tasked to conduct a survey of the territory along the middle and lower Tsna river and collect seeds of wild perennial and annual forages. Coordinates of collecting sites were recorded and sites mapped together with the collecting mission route. Two districts of the Tambov and two districts of Ryazan provinces were explored. As a result, 93 seed samples were collected. Out of these, 47 samples were perennial forage grasses, 39 perennial and annual forage legumes, and 7 belonged to other families. The main part of legumes was represented by Medicago species (11 samples of Medicago falcata L., M. varia Mart., M. lupulina L.) and clover (11 samples of Trifolium hybridum L., T. incarnatum L., T. medium L., T. pratensis L., T. repens L.); among the grasses there were Bromopsis inermis (Leyss.) Holub, Poa angustifolia L., Dactylis glomerata L., and Phleum pratense L.

Variability in proximate composition, phytochemical traits and antioxidant properties of Iranian agro-ecotypic populations of fenugreek (Trigonella foenum-graecum L.)

Fenugreek (Trigonella foenum-graecum L.) is a multi-use annual forage legume crop that is widely used in food products such as syrup, bitter run, curries, stew, and flavoring. In the present study, morphological traits, proximate composition (moisture, crude fibre, protein, fat, carbohydrate, and energy value), total phenol and total flavonoid contents, and antioxidant properties of 31 Iranian agro-ecotypic populations of the plant was investigated. Among the leaf and seed samples studied, the seeds exhibited the high ash (3.94 ± 0.12%), fat (7.94 ± 0.78%), crude fibre (10.3 ± 0.25%), protein (35.41 ± 1.86%), and carbohydrate (50.5 ± 1.90%) content. In general, more energy value (kcal/100 g) was also obtained from the seed (318.88 ± 1.78–350.44 ± 1.27) than leaf samples (45.50 ± 1.32–89.28 ± 0.85). Antioxidant activity and power of leaf samples were ranged from 67.95 ± 0.05‒157.52 ± 0.20 μg/ml and from 45.17 ± 0.01‒361.92 ± 0.78 μmol Fe+2 per g dry weigh, respectively. Positive linear correlations between antioxidant activity and total phenolic compounds were observed. A significant correlation between proximate composition (dependent variable) and some morphological features (independent variable) was observed. Considerable variability in the studied traits among the plant samples can be interestingly used in further food and production systems.

Species richness and functional diversity enhance winter annual forage productivity and nutritive value

AbstractWinter annual forages can extend grazing into the cool‐season in the US southeast, however uncertainty regarding potential tradeoffs between winter annual mixtures versus monocultures exists. We examined increasingly species‐rich and functionally diverse winter annual forage mixtures at two Florida locations to evaluate impacts of including more plant species with distinct traits (e.g., different maturities and nitrogen [N] scavenging vs. N2‐fixing capabilities) on forage accumulation (FA), nutritive value, and evenness of forage distribution across the cool‐season compared with a rye (Secale cereale L.) monoculture. Furthermore, we quantified the N2‐fixing potential of winter annual legumes to measure their contribution to soil fertility and ecosystem N cycling. We found that greater species richness enhanced total cool‐season FA at both sites, ranging from 0.92 to 4.38 Mg DM ha−1 in Gainesville and 6.48 to 9.11 Mg DM ha−1 in Marianna. In Marianna, where legumes accounted for approximately 25% or more of the biomass accumulated by winter annual forage mixtures, legume inclusion enhanced forage shoot N concentration. Furthermore, >50% of the N in legume tissues derived from the atmosphere, supplying 0.18–2 kg N ha−1 in Gainesville and 30–52 kg N ha−1 in Marianna. Altogether, our data suggest that species richness enhanced winter annual forage production and legume inclusion improved forage nutritive value although these responses were site‐specific. Thus, our multi‐site study provides information that can guide decision‐making regarding appropriate winter annual forage selections to increase cool‐season forage production in the southeastern United States.

An improved reference genome for Trifolium subterraneum L. provides insight into molecular diversity and intra-specific phylogeny.

Subterranean clover (Trifolium subterraneum L., Ts) is a geocarpic, self-fertile annual forage legume with a compact diploid genome (n = x = 8, 544 Mb/1C). Its resilience and climate adaptivity have made it an economically important species in Mediterranean and temperate zones. Using the cultivar Daliak, we generated higher resolution sequence data, created a new genome assembly TSUd_3.0, and conducted molecular diversity analysis for copy number variant (CNV) and single-nucleotide polymorphism (SNP) among 36 cultivars. TSUd_3.0 substantively improves prior genome assemblies with new Hi-C and long-read sequence data, covering 531 Mb, containing 41,979 annotated genes and generating a 94.4% BUSCO score. Comparative genomic analysis among select members of the tribe Trifolieae indicated TSUd 3.0 corrects six assembly-error inversion/duplications and confirmed phylogenetic relationships. Its synteny with T. pratense, T. repens, Medicago truncatula and Lotus japonicus genomes were assessed, with the more distantly related T. repens and M. truncatula showing higher levels of co-linearity with Ts than between Ts and its close relative T. pratense. Resequencing of 36 cultivars discovered 7,789,537 SNPs subsequently used for genomic diversity assessment and sequence-based clustering. Heterozygosity estimates ranged from 1% to 21% within the 36 cultivars and may be influenced by admixture. Phylogenetic analysis supported subspecific genetic structure, although it indicates four or five groups, rather than the three recognized subspecies. Furthermore, there were incidences where cultivars characterized as belonging to a particular subspecies clustered with another subspecies when using genomic data. These outcomes suggest that further investigation of Ts sub-specific classification using molecular and morpho-physiological data is needed to clarify these relationships. This upgraded reference genome, complemented with comprehensive sequence diversity analysis of 36 cultivars, provides a platform for future gene functional analysis of key traits, and genome-based breeding strategies for climate adaptation and agronomic performance. Pangenome analysis, more in-depth intra-specific phylogenomic analysis using the Ts core collection, and functional genetic and genomic studies are needed to further augment knowledge of Trifolium genomes.

Integration of annual forage legume with maize for better feed availability of livestock in maize dominated mixed farming system of Southern Region, Ethiopia

This study was conducted at Dore Befano and Meskan district of Sidma regional state and Gurage zone of south nation, nationalities, and peoples of southern Ethiopia’s (SNNPR), where maize dominated. The trial was conducted to evaluate the outcome of under sowing lablab on the grain yield, Stover, and appropriate time and seeding rate of forage crop under sowing. The trial was implemented using a 3 by 3 factorial combination with two sole treatments in an RCBD with three replications. The treatments were sole Maize (T1). Sole forage (T2) and Maize & Forage Simultaneous with maize sowing date full Simultaneous with maize sowing date 75%, Simultaneous with maize sowing date at 50%, Maize & Forage After 15 days of maize sowing full, after 15 days of maize sowing 75%, After 15 days of maize sowing 50%, Maize & Forage After 30 days of maize sowing full, Maize & Forage After 30 days of maize sowing 75% and Maize & Forage After 30 days of maize sowing 50% T3 to T11 respectively. Under sowing of lablab with maize did not affect the grain yield of maize. Among experiments, T5 was a more appropriate seeding rate and sowing time for under sowing of lablab with maize.

Evaluating warm‐season annual forages to fill summer forage gaps in short‐season climates

AbstractAnnual warm‐season forages have been used to fill productivity and nutritional gaps during summer months throughout the southeastern United States. However, their performance and nutritive value in cooler, short‐season temperate climates above 40 °N has been less well studied. This study evaluated the forage mass and nutritive value of four warm‐season annual forages {brown midrib [BMR] sudangrass [Sorghum bicolor ssp. Drummondii (L.) Moench], buckwheat (Fagopyrum esculentum Moench), Japanese millet [Echniochloa esculenta (A. Braun) H. Scholz], and teff [Eragrostis tef (Zuccagni) Trotter]} compared with a cool‐season small grain forage, oat (Avena sativa L.), and a cool‐season annual forage legume, chickling vetch (Lathyrus sativus L.). Monocultures of the six annual forage species, along with a mixture of all six species, were evaluated over 2 years in southeastern New Hampshire. Forage mass and nutritive value were assessed at weekly intervals over 3 weeks in July–August, approximately 4‐to‐5 weeks post‐germination. Across all three harvests, buckwheat, Japanese millet, and the summer mix produced greater forage mass than oat, while chickling vetch and teff produced the least. Conversely, chickling vetch had greater crude protein (CP), acid detergent fiber, and neutral detergent fiber compared with oat, while buckwheat had the lowest CP, in vitro true dry matter digestibility, and in vitro neutral detergent fiber digestibility of all the species assessed. Japanese millet produced greater forage mass while providing similar nutritive value as oat, making it a good alternative warm‐season annual forage for use under cool, short growing seasons.

Light interception, agronomic performance,and nutritive quality of annual forage legumes as affected by shade

Annual legumes can adapt to reduced light, a condition common in intercropping, by increasing the efficiency with which they convert energy into dry matter (DM). The performance of five forage legumes under reduced light–simulated by growing them under a shade net was evaluated in terms of agronomic adaptation, light interception, and the quality of forage. A third of the plot length was covered with Aluminet shade cloth 20–25 days after planting to reduce sunlight reaching the crop canopy to 30% of that without shade. Leaf area index (LAI) and light interception (LI) were significantly different depending on the species and shading up to 50–60 days after planting but not thereafter. Shading reduced DM significantly (p = 0.05), by 31–39% in 2009 and by 26–35% in 2010. Lima bean was affected the most (DM reduced by 43%), followed by pigeonpea (39%), whereas lablab (17%) and cowpea (29%) were affected the least. It affected dry matter in leaves and pods more than that in the stem. Shading had only a marginal influence on the concentration of crude protein,but increased the content of acid detergent fiber and neutral detergent fiber. Overall, lablab and cowpea were best adapted to reduced sunlight and therefore will be suitable for intercropping • Shade has a significant effect on canopy development and dry matter of all the legume species. • Shade has a significant effect on leaf chlorophyll, light interception, and leaf area index. • Impact of shade varies with legume species and affects relative feed quality, including digestibility.

High diversity of responses among Medicago truncatula lines to Phoma medicaginis infection.

Medicago truncatula is an omni-Mediterranean species grown as an annual forage legume. In addition to its small genome size and simple genetics, M. truncatula harbors several attributes, which make it an attractive model forage plant. In this study, we investigated the variation of responses in ten parental lines of M. truncatula to Phoma medicaginis infection. Plants were cultivated in the growth chamber under controlled conditions and were inoculated after two months with P. medicaginis. At harvest, 13 quantitative traits of growth and pathogenicity were measured. Results from ANOVA showed that the variation of analyzed parameters was explained by the effect of line. All measured parameters, except the root fresh weight, showed significant difference among the 10 studied lines. Most tolerant lines are those with the lowest ratios of the number of infected and dead leaves. Studied lines were clustered into three groups according to their responses to P. medicaginis infection. The most resistant TN6.18 line and most sensitive F83005.5 to P. medicaginis are useful for the exploration of physiological mechanisms and genetic determinants for M. truncatula tolerance to this constraint.

Significant cell differences in pod ventral suture in shatter‐resistant and shatter‐susceptible common vetch accessions

AbstractCommon vetch (Vicia sativa subsp. sativa L.) is a self‐pollinating annual forage legume with high nutritional value. However, pod shattering can cause severe yield loss at maturity and therefore is a significant trait for crop improvement. Our previous studies indicated that the ventral suture is critical to pod shattering in common vetch. To better understand the anatomy of the ventral suture, we evaluated eight shatter‐susceptible and 18 shatter‐resistant accessions for pod dehiscing characteristics over three consecutive years by comprehensively comparing the vascular bundle anatomical structure of the ventral suture. Four traits, specifically vascular bundle cell volume, bundle cap slope, single vascular bundle width, and external valve margin cells, were significantly different between the shatter‐susceptible and ‐resistant accessions. We found that the shatter‐resistant common vetches had a larger vascular bundle cell volume and bundle cap slope, an increased single vascular bundle width, and external valve margin cells that were significantly thicker than those of shatter‐susceptible accessions. All of the shatter‐susceptible pods had clear abscission layers. On the basis of these data, we propose a seesaw model of pod shattering in common vetch. Our morphological traits provide a novel insight into the pod shattering mechanism of common vetch, which will be valuable for breeding shatter‐resistant legumes.

“Summer sowing”: A successful innovation to increase the adoption of key species of annual forage legumes for agriculture in Mediterranean and temperate environments

AbstractThis paper reports on the evaluation of “summer sowing,” an innovative approach to increase the adoption of recently domesticated species of hard seeded annual legumes in Mediterranean and temperate Agriculture. The research revealed that several species of annual legumes whose seed can be readily harvested on‐farm and which possess natural hard seed dormancy, may be sown into dry soil in late summer without additional processing. These studies proved that the hard seed dormancy was broken down sufficiently in the soil over 4–6 weeks to produce robust legume pastures with more than 150 seedlings per m2 following the first winter rains, in replicated field sites established across wide agro‐ecological zones in Western Australia (WA) and New South Wales (NSW). Ornithopus sativus Brot., O. compressus L. and Trifolium spumosum L. were suitable for summer sowing based on both hard seed breakdown patterns and subsequent seedling survival in WA. While in NSW, in addition to these legumes, Biserrula pelecinus L., T. vesiculosum Savi. and T. glanduliferum Boiss. were also suitable for summer sowing. A 1.5‐ to 10‐fold increase in herbage production was achieved relative to conventionally sown T. subterraneum L. This development represents a step change in forage legume development for renovated pastures in these environments. Importantly, the experiments revealed differences in G x E effects on seedling establishment, total herbage production and seed yield in different climatic zones. The summer sowing approach is presented as a revolutionary method for pasture renovation that overcomes significant barriers to adoption.

Hard seed breakdown patterns of unprocessed forage legume seed sown into dry soil in summer in southern Australia

AbstractThe patterns and extent of hard seed breakdown of dormant seeds and pods following burial in the soil in February (summer) varied for up to 19 accessions of 12 species of annual forage legumes. The experiments, at six sites across southern Australia, were designed to identify legumes whose patterns of hard seed breakdown would suit them to agronomic evaluation in a summer sowing research program. For a subset of accessions, sufficient hard seed breakdown was demonstrated between February and April to allow a high proportion of seed to germinate in late autumn—a desirable pattern for summer sowing. Although there was a large G x E interaction, some cultivars of Ornithopus sativus Brot., O. compressus L. and Trifolium spumosum L. had a pattern that was reproducible at all sites. The experiments also identified accessions of Biserrula pelecinus L. and T. glanduliferum Boiss., which had the desirable pattern in regions of south‐eastern Australia, but not Western Australia. A pragmatic approach for selection of forage legume accessions in situ for evaluation within a summer sowing program was demonstrated.

Canadian sainfoin and fenugreek as forage and functional foods

AbstractSainfoin (Onobrychis viciifolia Scop.) and fenugreek (Trigonella foenum‐graecum L.) are two legumes that are being developed as forage crops in Canada with potential benefits for animal and human health. Sainfoin, a perennial crop containing condensed tannins (CTs), is gaining popularity in western Canada because of its benefits for cattle. Its CTs make the crop bloat‐free for grazing cattle while improving protein digestibility and reducing greenhouse gas emissions. The CT‐containing fenugreek is also considered as a bloat‐free annual forage legume that was developed to serve in short‐term crop rotations in western Canada. These crops are known to provide health and nutritional benefits to cattle with their high protein content and other beneficial nutraceuticals such as crude fiber, 4‐hydroxyisoleucine, steroid sapogenins, and galactomannans. Some of these nutraceuticals have the potential to benefit human health; however, such attributes have not been studied enough to harness the full potential of these legume crops in Canada. Recent research suggests that legumes are a healthy substitute for meat. However, metabolite analysis of sainfoin is mostly limited to proteins and CTs. The CTs reported in sainfoin are involved in reduction of blood pressure and detoxification and providing anticancer properties in humans. Recent studies on fenugreek have highlighted the beneficial nutraceuticals associated with human health but most of those claims are not backed by relevant clinical studies. In this article, we reviewed the nutritional quality attributes of sainfoin and fenugreek and assessed their potential as functional foods and nutraceuticals for animal and human health based on scientific evidence.

Mixture cropping of berseem clover with cereals to improve forage yield and quality under irrigated conditions of the Mediterranean basin

Mixture cropping of annual forage legumes and grasses is a common strategy to support sustainable forage supply in low input agricultural systems, especially in the Mediterranean basin. In a two-year field study, conducted in Northern Egypt, productivity and nutritive value of four cuts of berseem clover, triticale, and oat, cultivated as monocultures as well as legume-grass binary mixtures, with variable mixing rates, were investigated using a split plot design in three replicates. The tested mixing rates were: 1. 0% Grass (G) + 100% Berseem clover (BC), 2. 25% G + 75% BC, 3. 50% G + 50% BC, 4. 75% G + 25% BC, and 5. 100% G + 0% BC. Berseem clover mixtures with triticale produced the highest significant 3rd cut fresh yield, while BC mixtures with oat were superior at the 1st and 2nd cut's fresh yield. Crude protein (CP) content was highest in BC monocultures and clover-triticale mixture (75%:25%). Grasses, in general, improved the dry matter accumulation and carbohydrate components in the forage mixtures, with triticale being superior to oat. The significantly highest digestible organic matter (DOM) was a character of the pure BC stands, and mixtures with 75% BC. Noticeably, the tested grasses did not regrow after being cut for the third time; thus, the fourth cut was composed only of BC. Variations in DOM were most dependent on variations in CP content. Results revealed that mixtures of BC with triticale at 75%:25% mixing rate produced high forage yield with improved quality than the other mixtures.

First Report of Powdery Mildew Caused by Erysiphe viciae-unijugae on Vicia sativa subsp. nigra in Korea.

Vicia sativa subsp. nigra, also known as narrow-leaved vetch, belongs to the family Fabaceae and is distributed in different countries.Vetch is one of the most important annual forage legumes in the world; it is used as hay, grain, silage, green manure, and livestock fodder. In Korea, this plant is often used as cattle feed. In early May of 2019 and 2020, a powdery mildew was observed on leaves of V. sativa subsp. nigra plants in a garden of Chonnam National University, Gwangju, Korea. The first symptom appeared on the upper surface of the leaves as a small white spot. Within two weeks, the white spots of powdery mildew spread and covered the entire leaf. Voucher specimens were deposited at the Environmental Microbiology Laboratory Herbarium, Chonnam National University, Gwangju, Korea, under the accession numbers CNUFC PWV1 and CNUFC PWV2. The hyphal appressoria of the fungus were nipple-shaped, moderately lobed or multilobed, single or in opposite pairs. Measurements were made of 35 conidiophores and 55 conidia. Conidiophores arising from the upper part of mother cells, were cylindrical, 60.5 to 117.5 × 7.5 to 9.5 μm. The foot cells of the conidiophores were cylindrical, straight to slightly curved at the base, 23.5 to 38.7 × 7.3 to 9.2 μm, followed by 2-3 shorter cells. Conidia were formed singly, subcylindrical to oval, 32.5 to 48.7 × 13.5 to 19.7 μm. Conidia produced short to long germ tubes, terminating in lobed or unlobed appressoria. No chasmothecia were observed. The morphological characteristics of conidiophores and conidia were consistent with the description of Erysiphe viciae-unijugae in Shin (2000). However, it was not possible to identify the fungus only based on the asexual morph. In addition, the conidiophore and conidium shapes of E. baeumleri on the known host V. sativa were similar to those of E. viciae-unijugae (Braun and Cook 2012). Therefore, genomic DNA was extracted directly from the mycelia and conidia using a DNA prep kit (Solgent, Daejeon, Korea). The internal transcribed spacer (ITS) regions ofribosomal DNA (rDNA) and 28S rDNA from the two specimens were amplified using the primers ITS5 and PM6 (Takamatsu and Kano 2001), and LROR and LR3 (Vilgalys and Hester 1990), respectively. A BLASTn search with the ITS sequences indicated that CNUFC PWV1 (GenBank accession no. MT509395) and CNUFC PWV2 (MT509396) were 100% (503/503 bp) and 99.8% (502/503 bp) identical to those of E. viciae-unijugae (LC010086 and LC009962), respectively. The 28S sequences of CNUFC PWV1 (MT705629) and CNUFC PWV2 (MT705630) showed 99.8% (507/508 bp) and 99.4% (505/508 bp) identity values with E. viciae-unijugae (LC009976 and LC010029), respectively. Phylogenetic analyses based on the combined ITS and 28S rDNA sequences indicated that the two specimens, CNUFC PWV1 and CNUFC PWV2, formed a monophyletic clade together with sequences retrieved from E. viciae-unijugae. Therefore, the causal pathogen was identified as E. viciae-unijugae based on the results of morphological and molecular analyses. Pathogenicity tests were performed by gently pressing the infected leaves onto 10 young leaves of three healthy plants. A non-inoculated plant was used as a control. The plants were maintained in a greenhouse at 22 to 25°C, with relative humidity around 75%, and natural light. Powdery mildew symptoms were observed on the inoculated leaves after 10-14 days, whereas no symptoms were observed on the leaves of the control. The fungi on the inoculated leaves were morphologically identical to the fungi on the original infected leaves. Erysiphe viciae-unijugae has been reported to occur on Lathyrus spp. and Vicia spp. (Farr and Rossman 2020). To our knowledge, this is the first report of powdery mildew caused by E. viciae-unijugae on V. sativa subsp. nigra in Korea. The weather conditions, especially during May, are favorable for disease outbreaks. This disease may be a potential threat to feed production in Korea.

Forage legumes in agricultural production systems

In order to increase and intensify of livestock production, the solution should be found in high-yielding plant species of good quality, which fit into the system of continuous production of animal feed. Together with efforts to reduce energy consumption, environmental pollution, intensify sustainable agriculture systems and maintain biodiversity, the possibility of increasing areas under forage legumes should be considered. As nitrogen fixers, these plants are minimally fertilized with nitrogen fertilizers whose residues in soil are lost by leaching, causing pollution of groundwater as well as surface watercourses. The diversity of their use, high and stable yield and quality of products, make these plants important in solving problem of deficit of protein-rich animal feed. From the group of perennial forage legumes, the representatives with the greatest economic and agrotechnical importance are: blue alfalfa, sickle medick, hybrid alfalfa, black medic, red clover, white clover, birdsfoot trefoil, greater bird's-foottrefoil, narrow-leaved bird's-foot-trefoil, common sainfoin, white sweet clover and yellow sweet clover. In the group of annual forage legumes that are grown or may be of interest in modern forage production systems belong to: vetchs, forage peas, broad bean, lupins, soybeans and cowpea. Besides until recently, far more cultivated species such as lentils, grass pea and bitter vetch, positive results of preliminary experiments with species such as pigeon pea, white lupin, narrowleaf lupin, narbon bean and cowpea, indicate the possibility of their introduction into livestock production systems and / or green manure. Forage legumes are grown in Republika Srpska on about 50,000 ha as pure crops, often as combined crops with perennial or annual grasses, other legumes or plants from other groups. Growing combined leguminous crops and grasses or cereals can provide many benefits, such as: increased biomass yield and utilization of soil resources, increased yield stability, improved nutrient, water and light utilization, as well as weed, disease and pest control. In the temperate climate zone, this way of cultivation is far more successful when used in forage production compared to grain production. Today, as in the previous period, there is a need for species and cultivars of forage legumes, which in addition to with high yield potential, will have improved grain / forage quality, as well as increased resistance to stress, especially stress caused by lack of soil water, high temperatures and increased solar radiation. The economy of production of legumes at the farm level is often misjudged as negative, because they generally do not take into account the positive, economically currently invisible, advantages in growing legumes. Prices of protein feed underlying the intensive production systems in the future will continue to rise, so that in the near future, cultivation of legumes and be cost effective than most other agricultural plants. The benefits of cultivating legumes and their impact on the advancement of agroecosystems have been for a long time. The introduction of legumes into production systems would limit the increasing degradation of land. In order to develop sustainable agriculture, market policy should recognize the value of products obtained from leguminous plants by certain agrarian policy measures.

YIELD, QUALITY AND COMPETITION PROPERTIES OF GRASS PEA AND WHEAT GROWN AS PURE AND BINARY MIXTURE IN DIFFERENT PLANT DENSITIES

Mixtures of annual forage legumes with winter cereals for forage are practiced traditionally in the Mediterranean conditions. The objectives of this study were to compare grass pea and wheat pure stands as well as their mixtures in different plant densities under two seeding ratios for forage yield and quality and to estimate the effect of competition between the two species used in the intercropping systems. The experimental design was split plot in a randomized complete block with three replications. The main plot treatments were plant densities (350, 500 and 650 plant m-2) and sub-plots treatments were four sowing norm (pure grass pea (GP), 60% GP+ 40% W, 70% GP+ 30% W and pure wheat (W)). The results of the present study showed that plant densities do not have a significant effect on forage yield and quality, while the effect of mixture and pure stands on forage yield and quality were significant. According to the two-year average results, 60% GP + 40% W mixture gave the best results in terms of yield and quality. Calculated LER values showed that wheat+grass pea mixtures were more advantageous than pure stand of both species. As result of this research, 350 plant m-2 plant density and 60% GP + 40% W mixture can be recommended.

Coordinated mechanisms of leaves and roots in response to drought stress underlying full-length transcriptome profiling in Vicia sativa L

BackgroundCommon vetch (Vicia sativa L.) is an important self-pollinating annual forage legume and is of interest for drought prone regions as a protein source to feed livestock and human consumption. However, the development and production of common vetch are negatively affected by drought stress. Plants have evolved common or distinct metabolic pathways between the aboveground and underground in response to drought stress. Little is known regarding the coordinated response of aboveground and underground tissues of common vetch to drought stress.ResultsOur results showed that a total of 30,427 full-length transcripts were identified in 12 samples, with an average length of 2278.89 bp. Global transcriptional profiles of the above 12 samples were then analysed via Illumina-Seq. A total of 3464 and 3062 differentially expressed genes (DEGs) were identified in the leaves and roots, respectively. Gene Ontology (GO) enrichment analyses identified that the dehydrin genes and Δ1-pyrroline-5-carboxylate synthase were induced for the biosynthesis of proline and water conservation. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis results indicated that the DEGs were significantly enriched in hormone signal transduction, starch and sucrose metabolism, and arginine and proline metabolism, and various drought response candidate genes were also identified. Abscisic acid (ABA; the AREB/ABF-SnRK2 pathway) regulates the activity of AMY3 and BAM1 to induce starch degradation in leaves and increase carbon export to roots, which may be associated with the drought stress responses in common vetch. Among the co-induced transcription factors (TFs), AREB/ABF, bHLH, MYB, WRKY, and AP2/ERF had divergent expression patterns and may be key in the crosstalk between leaves and roots during adaption to drought stress. In transgenic yeast, the overexpression of four TFs increased yeast tolerance to osmotic stresses.ConclusionThe multipronged approach identified in the leaves and roots broadens our understanding of the coordinated mechanisms of drought response in common vetch, and further provides targets to improve drought resistance through genetic engineering.

Evaluation of accessions of vetch species for agronomic performance under nitosol and vertisol conditions in the central highlands of Ethiopia

Vetch is an annual forage legume and accessions of its species performed differently under different soil conditions. Twenty accessions of five vetch species were evaluated for their agronomical differences at Holetta and Ginchi in the central highlands of Ethiopia. The rate of DM accumulation was similar during the initial growth stage. However, after the second sampling (74 DAS), aerial DM accumulation varied widely across the testing sites due to the difference in temperature, rainfall, and soil fertility conditions. The average stretched plant height was higher at Ginchi compared to Holetta, which could be attributed to higher and extended rainfall and favorable growing conditions during the experimental period. In addition to genetic variability, soil fertility and environmental conditions could also contribute to the difference in height. On average, about 10 and 20 more days were required to harvest forage and seed yield, respectively, at Ginchi compared to Holetta. Days to forage harvest ranged from 80.7 to 113.0 and 94.3 to 126.0 days after the emergence of the seedlings at Holetta and Ginchi, respectively. The difference in maturity is an important agronomic trait to select companion crops for maximum production. The higher biomass production rate was recorded at Ginchi than in Holetta. Accessions of early maturing vetch species had a comparatively higher biomass production rate, while late-maturing accessions had lower biomass production rates at both locations. Intermediate-to-late-maturing accessions of vetch species gave relatively better forage DM yield than early maturing accessions at both locations. The highest grain-filling period was recorded for accession 2424 (Vicia villosa) at Holetta and accession 64266 (Vicia sativa) at Ginchi. Vetch accessions showed higher seed yield and thousand seed weight at Ginchi than Holetta. The 20 accessions of vetch species were grouped into three clusters based on major measured parameters.

Understanding Why Effective Fungicides Against Individual Soilborne Pathogens Are Ineffective with Soilborne Pathogen Complexes.

Annual forage legumes across southern Australia continue to be devastated by soilborne diseases. Nine fungicide seed treatments (thiram, metalaxyl, iprodione, phosphonic acid, propamocarb, fluquinconazole, difenoconazole + metalaxyl, ipconazole + metalaxyl, sedaxane + difenoconazole + metalaxyl) and four foliar fungicide treatments (phosphonic acid, metalaxyl, propamocarb, iprodione) were tested on four subterranean clover cultivars against individual oomycete soilborne pathogens Pythium irregulare, Aphanomyces trifolii, and Phytophthora clandestina and the fungal pathogen Rhizoctonia solani. Best treatments were then further tested across southern Australia in 2 years of field experiments. Under controlled conditions, seed treatment with thiram was best against damping-off caused by P. irregulare across the four cultivars (Woogenellup, Riverina, Seaton Park, Meteora), while metalaxyl was the most effective for maximizing root and shoot weights. Against A. trifolii, metalaxyl, iprodione, difenoconazole + metalaxyl, ipconazole + metalaxyl, and sedaxane + difenoconazole + metalaxyl, all reduced damping-off; sedaxane + difenoconazole + metalaxyl, fluquinconazole, and ipconazole + metalaxyl all reduced lateral root disease across two or more cultivars; while iprodione, thiram, and sedaxane + difenoconazole + metalaxyl increased shoot dry weight. Against P. clandestina, metalaxyl was the most effective in reducing tap and lateral root rot followed by ipconazole + metalaxyl or phosphonic acid for tap and lateral rot, respectively. Against R. solani, there were no effects of fungicides. For P. irregulare and P. clandestina, there were strong seed fungicide × cultivar interactions (P < 0.001). Under controlled conditions for foliar fungicide spray treatments, phosphonic acid was best at preventing productivity losses from A. trifolii, but was ineffective against P. clandestina, P. irregulare, or R. solani. Overall, controlled environment studies highlighted strong potential for utilizing seed treatments against individual pathogens to ensure seedling emergence and early survival, with seed and foliar sprays enhancing productivity by reducing seedling damping-off and root disease from individual pathogens. However, in field experiments over 2 years across southern Australia against naturally occurring soilborne pathogen complexes involving these same pathogens, only rarely did fungicide seed treatments or foliar sprays tested show any benefit. It is evident that currently available fungicide seed and/or foliar spray treatment options do not offer effective field mitigation of damping-off and root disease on annual forage legumes that underpin livestock production across southern Australia. The main reason for this failure relates to the unpredictable and ever-changing soilborne pathogen complexes involved, highlighting a need to now refocus away from fungicide options, particularly toward developing and deploying new host tolerances, but also in deploying appropriate cultural control options.