ObjectiveThe aim of this study was to investigate the association between serum 25(OH)D level and quantitative measures of skin color along with a self‐reported sun exposure score.MethodsThis cross‐sectional study assessed the serum 25(OH)D level among 75 multi‐ethnic adult males (n=39) and females (n=36), aged 18–36 years living in South Florida. The skin color was quantified by a SMARTPROBE400 scanner and 25(OH)D was measured by ELISA method. A sun exposure questionnaire was used to determine the average annual sun exposure score. A linear regression model adjusted for age, gender, race, body mass index, alcohol consumption, tobacco use and years living in US was run.ResultsTotal sun exposure score and forearm skin color were predictors of serum 25(OH)D level (P=.004 and P=.004 respectively). This association held after controlling for covariates (B=.292, P=.027 for forearm and B=.385, P=.005 for total sun exposure score). The model explained 24.6% of the variation in 25(OH)D.ConclusionThe outcome of this study suggested skin color measures and sun exposure scores can be used as an indirect non‐invasive tool to estimate serum 25(OH)D level.