Animal Well-being Research Articles

Influence of neonatal feeding on zootechnical and economic aspects of the initial production of broiler chickens

The aim of the present study was to evaluate the influence of neonatal feeding on zootechnical and economic aspects of the initial production of broiler chickens. A total of 320 Cobb-500 male chicks were used. After vaccination, the neonatal hydrating feed was added to the transport box. Four control groups were formed (A to D) with chicks 8, 24, 48 and 72 hours after hatching without neonatal feeding and four experimental groups were formed (E to H) with chicks lodged 8, 24, 48 and 72 hours after hatching with neonatal feeding. The hydrating supplement demonstrated dehydration of the chicks. Group E had an initial body weight gain of 1.88% associated with a shorter post-hatch lodging interval. At seven days of age, the chicks in Group F, which received neonatal feeding, were an average of 15.31 g above the expected weight. Based on yield rates at seven and 14 days, the average live chicken gross margin and average carcass gross margin were positive in birds that received neonatal nutrition. Neonatal supplementation offers a financial benefit by enhancing animal wellbeing and contributing to an increase production profit.

Primeiro caso de Entamoeba em equino no Distrito Federal

This study reports the first confirmed case of infection by Entamoeba sp. in an equine in the Federal District. An English Thoroughbred equine showed non-specific clinical signs, such as weight loss and behavioral changes. The parasitological examination of the feces revealed the presence of Entamoeba sp., in addition to other helminths. After diagnosis, the animal was treated with moxidectin and praziquantel, resulting in the elimination of Entamoeba sp. This case demonstrates the importance of parasitological diagnosis in horses, even in those with nonspecific clinical signs. Entamoeba sp. in horses it is a rare finding and highlights the need for additional studies to better understand the epidemiology and impact of this parasitic disease on equine health. Furthermore, the case highlights the importance of an accurate diagnosis and adequate treatment to guarantee the health and well-being of animals.

Brighton v RSPCA NSW: Appeals and Lessons Four Years On.

Animal law has the potential to initiate improvements for animal wellbeing. However, this largely depends on how effectively the law bridges the legal chasm between animal welfare and animal suffering, a chasm the authors refer to as the welfare gap. When the law does not adequately address this gap, where regulation subordinates animal interests to human interests, it results in weak animal protection that does little more than regulate to a standard that avoids a life not worth living. The authors analyse a series of cases involving the RSPCA and Brighton, in which Brighton was charged with serious animal cruelty pursuant to s 530 of the Crimes Act 1900 (NSW). He stabbed a dog with a pitchfork; after failing to kill the dog, he suspended it from a tree branch by a leash attached to its collar and struck the dog several times on the head with a mallet, finally killing him. Brighton was found guilty in the NSW Local Court and appealed to the NSW Supreme Court, where Rothman J allowed the appeal, holding that Brighton had exterminated a pest animal. This led to protracted litigation, including to the NSW Court of Appeal, a second hearing in the Local Court and a further appeal to the Supreme Court. In August 2020, Sophie Riley published a case note and commentary on the litigation up to the Rothman J appeal. This paper evaluates the litigation that followed, identifying how regulatory failures have entrenched the welfare gap. Regulatory failures include inadequate and aged legislative protections for a confined subset of animals. In NSW, animal sentience is not enshrined in legislation; the law limits the types of animals protected by anti-cruelty law; fundamental statutory language remains undefined, for example terms such as "pest animal" and "exterminate"; and challenges abound for adducing sufficient evidence to prove subjective criminal intent. These deficiencies pose significant challenges for practitioners and judicial officers, particularly when complex statutory interpretation is required in the busy and fast-paced summary jurisdiction. This paper concludes that legislators should consider modernising the law, removing ambiguity, and settling minimum standards for a good life for animals, taking into account the welfare aspects described in Mellor's Five Domains model.

Simplifying the Animal Welfare Assessment Grid for enhanced accessibility.

Ensuring animal welfare is essential for both the well-being of zoo animals and the effective management of zoological facilities. This study introduces the Simplified Animal Welfare Assessment Grid (S-AWAG), a streamlined adaptation of the original AWAG framework that integrates the Five Domains Model with an observation-based approach. Designed for non-expert users, S-AWAG focuses on easily observable welfare indicators-such as health and environmental conditions-making it particularly suitable for small, private zoos, including petting zoos, roadside zoos, indoor zoos, and animal cafés. We conducted welfare assessments on 304 animals from 11 species across nine zoos in South Korea. The results revealed significant differences in welfare standards between accredited and non-accredited zoos, with accredited facilities consistently demonstrating better welfare conditions (p < 0.05). The tool exhibited high inter-rater reliability (IRR = 0.839), confirming its consistency across assessors with varying levels of expertise and ensuring reliable and accurate results. Pearson correlation analysis identified strong positive associations between health and environmental factors, reinforcing the comprehensive nature of the tool's evaluation approach. With its user-friendly, efficient, and adaptable design, S-AWAG has the potential to improve animal welfare standards not only in South Korea but also globally, particularly in smaller, resource-constrained facilities.

Attenuated Getah virus confers protection against multiple arthritogenic alphaviruses.

Alphaviruses are important arthropod-transmitted pathogens of humans and livestock. Getah virus (GETV) is an arthritogenic alphavirus that causes disease in horses and piglets; it also poses a potential threat to humans. A live attenuated vaccine candidate named GETV-3ΔS2-CM1, harbouring a deletion in nonstructural protein 3 and substitutions in the capsid protein, is genetically stable and exhibits robust immunogenicity. It was shown to confer passive protection to piglets born to immunized sows. In mice, a single dose of GETV-3ΔS2-CM1 protected against infection with different strains of GETV, Semliki Forest virus, Ross River virus, o'nyong'nyong virus, chikungunya virus, and Barmah Forest virus. Chimaeras based on the GETV-3ΔS2-CM1 backbone maintained both the attenuated phenotype and high immunogenicity. The safety, efficacy, and ability to induce protection against multiple alphaviruses highlights the potential of GETV-3ΔS2-CM1 and chimaeras using this backbone as promising vaccine candidates. By contributing simultaneously to the wellbeing of animals and humans, our universal next generation vaccine strategy helps to achieve "One Health" goals.

Dog owners and preventative healthcare: knowledge level, interest, and impact on perceptions of veterinarians' trustworthiness and empathy.

Gain a better understanding of dog owners' knowledge and interest level for 13 preventative healthcare (PHC) topics. Examine the potential predictive value of owners' satisfaction with how their veterinarian communicates about PHC topics and perceived veterinarian-related trust and empathy and frequency of PHC veterinary visits. An electronic survey was created in Qualtrics and distributed to dog owners within the US via a link offered in Prolific, an open online marketplace whereby respondents receive small monetary compensation for completing surveys. A total of 738 participants completed the survey, all of whom were dog owners living in the US. Owners reported knowing the most about the importance of mental engagement, how to assess their dog's everyday quality of life, vaccines, and parasites. Owners were most interested in learning more about how to assess their dog's everyday quality of life, importance of mental enrichment, diseases related to their dog's breed, and senior care. Veterinarians were the primary source of information for 13 of 14 topics. Owners' satisfaction with how PHC information is discussed was a significant predictor for perceived veterinarian empathy and trustworthiness, as well as frequency of PHC visits. Dog owners understand the value of PHC and look to their veterinarian for this information. Satisfaction with PHC dissemination predicts owners' views of their veterinarian's trustworthiness and empathy. Dissemination of PHC information can improve animal health and well-being not only directly but also indirectly through its impact on owners' perceptions of their veterinarian.

Climate Change and Veterinary Medicine: A Call to Action for a Healthier Planet

Abstract Climate change is rapidly transforming ecosystems and reshaping the landscapes of animal health, with profound consequences for public health, food security, and biodiversity. Rising temperatures, shifting weather patterns, and increased frequency of natural disasters are driving the emergence and spread of infectious diseases, particularly zoonotic and vector-borne diseases. These environmental shifts endanger the health and welfare of animals and the delicate balance between human populations, livestock, and wildlife. As the stewards of animal health, veterinarians are uniquely positioned to lead the change in addressing these complex challenges at the nexus of human, animal, and environmental health and well-being. This article calls for urgent actions to integrate climate adaptation and mitigation strategies into veterinary practice and education. It underscores the critical need for veterinarians to embrace the One Health approach to tackle climate-driven disease outbreaks and the growing threat of antimicrobial resistance to safeguard human and animal populations while protecting natural ecosystems. The article further explores the role of veterinarians in fostering sustainable agricultural practices, reducing the environmental impact of livestock production, conserving biodiversity and advocating for policy reforms that protect both animal and planetary health. As we face an era of unprecedented climate disruption, this call to action aims to inspire the global veterinary community to actively get involved in combating climate change and its worst impacts. By building climate-resilient practices, enhancing disease surveillance, and championing environmental stewardship, veterinarians can contribute significantly to a healthier, more sustainable future for all species on Earth.

The efficacy of acupuncture in animals – Part 1 Veterinary medicine use, historical background, theoretical foundations, techniques and tools, points and meridians and physiological mechanisms of acupuncture in animals

Holistic approach in veterinary has gained popularity in recent years. However, more research is needed to fully understand the effects of acupuncture (AP) in animals and to determine its safety and efficacy. This review work aimed to describe AP in animals, regarding the use in Veterinary Medicine, historical background, theoretical foundations, techniques and tools for animals, AP points and meridians and physiological mechanisms of AP in animals. Although AP use in animals has been practiced for thousands of years, scientifically efficacy based studies are still lacking in the research community. This ancient traditional Chinese method involves inserting thin needles in specific acupoints to induce vital energy balance across skin meridians to produce biological responses from the nervous system. Understand the impact and potential healing powers that exist within these vital points is crucial, because AP can positively influence in health and well-being of animals. Musculoskeletal pain, respiratory issues, reproductive and metabolic disorders are the most frequent described AP treatments in veterinary practice. With proper application, AP can be a valuable tool in treating various health conditions and promoting well-being. It is important to consider potential risks, as well other treatment protocols and consult with a qualified veterinary before undergoing AP treatment.

Antibiotic contaminants and their impact in Gingee River, Puducherry: insights from SPE-UPLC-MS/MS and zebrafish study.

The accumulation of antibiotic residues in ecosystems is intricately tied to the proliferation of bacterial resistance to antibiotics, with far-reaching consequences for the health and welfare of both humans and animal well-being. The analytical approach integrates solid phase extraction (SPE) with ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS) for quantification of multiclass antibiotic residues. Upon applying the aforementioned method to analyse water samples collected from the Gingee River, revealed the existence of five distinct antibiotics. This is the first study reporting antibiotic concentration in the Gingee River, Puducherry. The concentrations of nalidixic acid, sulfamethoxazole, and tetracycline were determined to be 8.5, 6.9, and 4.8μg/L, respectively. Metronidazole and trimethoprim were detected at concentrations below the quantifiable limit. The microbial study of water samples also indicated that Shigella sp. and Acinetobacter sp. were the most predominant bacterial species present. Our preliminary observation underscores the importance of comprehending the intricate relationship between the presence of antibiotics in water and the concurrent proliferation of antibiotic-resistant bacteria within bacterial populations in the Gingee River. Further, we evaluated the developmental toxicity of environmentally relevant concentrations of antibiotics in zebrafish. The zebrafish model confirms that these antibiotics are sublethally hazardous to human health at environmentally relevant concentrations. This integrated approach allows unique views on the environmental impact of antibiotic residues, their role in the evolution of antibiotic resistance, and their impact on human health.

Chronic exposure to low concentration of diflubenzuron and pyriproxyfen induces brain oxidative stress and inflammation and alters locomotion in zebrafish (Danio rerio)

Diflubenzuron (DFB1) and pyriproxyfen (PPF) are pesticides widely used in agriculture and urban environments to control insect actions. The aim of this study was to evaluate the effects of chronic 30-day exposure to DFB (0.025 and 0.125 mg/L) and PPF (0.379 and 0.758 mg/L) on the behavior of juvenile zebrafish (Danio rerio). Fish were exposed to insecticides from early stage (4 h post fertilization) to 30 days post fertilization (dpf). At 45 dpf, fish were evaluated in the novel tank test, social behavior test, and mirror aggressive test. Brain gene expression related to oxidative stress and inflammation was also evaluated. DFB reduced locomotor parameters in the novel tank and aggression tests, while it induced to hyperactivity in the social behavior test. PPF reduced anxiety-like behavior, measured by the time spent in risky areas of the novel tank, and reduced aggression against the mirror image. There was a significant reduction in mRNA levels of the nfe2l2 gene (∼0.54 fold downregulated) and increase in levels of cat (PPF ∼1.8 fold change), gsr (PPF ∼1.5 fold change), gpx1a (PPF ∼1.6 and DFB 1.1 fold change), tnf-α (PPF 1.9 and DFB 2.2 fold change), and il-6 (PPF ∼1.2 and DFB 2.3 fold change). These endpoints are indicative of the threatening effects of insecticides to aquatic organisms and the need for alternative methods to control pests by using less harmful and safer substances for animal and human well-being, as well as for the environment.

Working on new topics: Estimation of the burden of zoonotic diseases

Abstract Zoonoses, diseases naturally transmitted between vertebrates and humans, represent significant global health threats. These diseases not only affect human health but also impose a considerable burden on animal populations, emphasizing the need for a One Health approach that integrates strategies across human, animal, and environmental health sectors. The socioeconomic impacts of zoonoses are profound in low- and middle-income countries, disrupting production systems, impacting livelihoods, and compromising food security. Populations in these regions are excessively affected, highlighting the urgent need for a comprehensive approach. The disability-adjusted life year (DALY) quantifies disease burden in terms of morbidity and mortality. The zoonotic DALY (zDALY) expands this metric by incorporating the burden on animals through the ‘animal loss equivalents’, accounting for both fatal and non-fatal outcomes in livestock. However, zDALYs do not encompass broader economic impacts associated with prevention and treatment. Cost of illness (COI) studies further quantify the financial costs due to health issues; ad hoc approaches can be used to quantify other costs related to animal disease control and eradication, trade and movement Implications, or loss of consumer confidence. While few zoonoses, such as cystic echinococcosis and brucellosis, have comprehensive data across these metrics, most do not. While these metrics provide complementary insights, no holistic metric currently encompasses all necessary aspects to fully assess the burden of zoonoses within a One Health framework. This presentation aims to offer state-of-the-art estimates of the disease burdens of zoonoses, employing DALY, zDALY, and COI studies. It will explore how to integrate these metrics from a One Health perspective. Indeed, only by integrating health and economic metrics that consider the well-being of humans and animals, can the One Health approach effectively be developed for zoonotic diseases.

Rethinking Animal Consciousness Research to Prioritize Well-Being.

The authors critique the NY Declaration on Animal Consciousness, which does not denounce continued captivity and invasive research in the pursuit of animal consciousness markers. They argue that such research often increases animal suffering by accepting harmful practices. Instead, they propose a nonanthropocentric, ethical framework aligned with the Belmont Report's principle of beneficence, advocating for noninvasive methods in natural habitats. This approach prioritizes animal well-being, recognizing and safeguarding the intrinsic value of all conscious beings.

Efeito do uso da Promixalicina no controle de verminose e parâmetros sanguíneos de ovelhas

Sheep producers are increasingly looking for strategies to tackle the problem of worms in animals, as well as to improve the functioning of the animals' immune system. Therefore, two experiments were carried out, in two different properties (with high and low challenges in relation to verminosis) with the objective of, in both, evaluating the effect of using Ovine Promixalicin on OPG (Eggs per gram of Feces) and blood parameters of pregnant and non-pregnant sheep, on two different properties. The indication for use of the product is to, among others, contribute to the control of verminosis. The animals evaluated on the properties came from a pasture production system, with the use of concentrated and/or roughage supplementation if necessary. The animals used were of the Dorper, Texel, Ile de France and Sulfolk breed. The parameters evaluated were OPG, Famacha©, Body Condition Score and blood parameters (lymphocytes, leukocytes and erythrocytes). In relation to experiment one, with a low challenge in relation to worms, the use of Promyxalicin significantly helped to reduce worms and animal well-being, thus helping the herd to increase productive parameters such as weight gain and fertility. In experiment 2, a system with a high challenge in terms of verminosis, the animals remained uniform and controlled by the use of Promyxalicin, including beneficial results in relation to blood parameters, which did not increase during its use. Therefore, the use of Ovine Promixalicin does not alter the consumption of food by animals, as well as being effective in controlling verminosis, including Haemonchus contortus, Trichostrongylus colobriformis, Oesophagostomum columbianum e Strongyloides papillosus, promoting well-being and maintenance of the immune system, especially in pregnant animals, bringing benefits to the sheep production system.

Outcomes of a Community Cat Program Based on Sterilization of Owned, Semi-Owned and Unowned Cats in a Small Rural Town.

Free-roaming cats in urban and peri-urban areas and in rural towns pose significant challenges due to their nuisance behaviors, threats to wildlife, and potential disease transmission. Traditional management methods often involve trapping and euthanizing many of these cats, which is not only costly but also distressing for the shelter staff involved, and have failed to reduce numbers of impounded cats or cat-related complaints. Our study aimed to evaluate the effectiveness of a Community Cat Program that provided free sterilization, microchipping, and preventative veterinary care for all owned, semi-owned, and unowned cats in a small rural town with high cat impoundments in Ipswich, Queensland. The program was instigated by the Australian Pet Welfare Foundation in collaboration with the Royal Society for the Prevention of Cruelty to Animals, Queensland and the Animal Welfare League, Queensland, and it was funded by various organizations including Fondation Brigitte Bardot. Data from 2017 to 2023 were analyzed to assess the impact of the program on cat intake and outcomes in local shelters. A total of 308 cats were sterilized, representing 94 cats per 1000 residents over 3.4 years, and in the third year, this was associated with a 60% decrease in cat intake, an 85% reduction in numbers euthanized and 39% fewer cat-related calls to the local council. These findings suggest that high-intensity, targeted sterilization programs significantly decrease shelter intake and euthanasia, and thus alleviate the psychological burden on staff. These programs are a humane and effective alternative to traditional cat management, and benefit animal welfare as well as community wellbeing. This is aligned with a One Welfare approach, which enhances the well-being of animals, humans, and their environments. Legislative changes are required to support these programs to effectively decrease the numbers of free-roaming cats in cities and towns and the issues they cause, while protecting human wellbeing.

Cooling Effectiveness of the Sustainable Cooling Solution for Cattle: Case Study in Poland

Recently, the dairy sector has been ever more affected by global warming. This study aimed to test a novel conductive cooling system for cattle that was successfully implemented and evaluated under summer thermally challenging weather conditions in Poland. The system consists mainly of the chiller, tank, and chilled water-driven mattress, designed to prioritize animal well-being. The experimental evaluation was carried out on three Friesian dry cows, housed on different types of bedding—commercial water mattress, straw, and cooling water mattress—and supplied with water at 10 °C (day) and 16 °C (night). The cooling water mattress’ surface temperature was twice as low as that of the commercial water mattress. The animal’s thermal comfort was assessed with physiological and behavioral reactions. The cooling effect on animals’ bodies was demonstrated with a lower reticulorumen temperature of the cooled cow (p &lt; 0.05) than the reference ones. The local effect of cooling was proved with an 8 °C-lower skin temperature after the cow’s resting period. The presented study opens a new research direction toward dairy cattle’s welfare, sustainability, and the food–energy–water nexus, based on potential energy and water savings.

Epithelial barrier dysfunction and associated diseases in companion animals: Differences and similarities between humans and animals and research needs.

Since the 1960s, more than 350,000 new chemicals have been introduced into the lives of humans and domestic animals. Many of them have become part of modern life and some are affecting nature as pollutants. Yet, our comprehension of their potential health risks for both humans and animals remains partial. The "epithelial barrier theory" suggests that genetic predisposition and exposure to diverse factors damaging the epithelial barriers contribute to the emergence of allergic and autoimmune conditions. Impaired epithelial barriers, microbial dysbiosis, and tissue inflammation have been observed in a high number of mucosal inflammatory, autoimmune and neuropsychiatric diseases, many of which showed increased prevalence in the last decades. Pets, especially cats and dogs, share living spaces with humans and are exposed to household cleaners, personal care products, air pollutants, and microplastics. The utilisation of cosmetic products and food additives for pets is on the rise, unfortunately, accompanied by less rigorous safety regulations than those governing human products. In this review, we explore the implications of disruptions in epithelial barriers on the well-being of companion animals, drawing comparisons with humans, and endeavour to elucidate the spectrum of diseases that afflict them. In addition, future research areas with the interconnectedness of human, animal, and environmental well-being are highlighted in line with the "One Health" concept.

Microbial Degradation of Pesticides in Agricultural Environments: A Comprehensive review of Mechanisms, Factors and Biodiversity

Pesticides are used frequently in modern times to prevent and manage crop pests and diseases, but their residual effects have seriously harmed both the environment and the health of humans. Within the fields of all over the world applied restoration of the environment science and technology, the study of microbial breakdown of pesticides in soil ecosystems is a significant research area. Agricultural methods depend on the use of chemicals, including pesticides and herbicides, to control pests and weeds. However, these chemicals pose risks to the environment, human, and animal well-being. Microbes have shown promise in degrading these agricultural environmental hazards, mitigating their negative impact. The organisms that exist in the natural world, the research on bacteria that break down pesticides and herbicides, and the approach for the application of these bacteria has been summarized in the paper. This literature review aims to identify the microbes responsible for degrading these chemicals and assess their effectiveness in doing so. The goal of this review is to determine the bacteria responsible for the deterioration of agricultural environmental hazard chemicals, evaluate their efficacy in degrading these compounds, explore the factors influencing microbial degradation efficiency, and identify research gaps in the field. Inclusion criteria encompass studies published in English between 2010 and 2023 that focus on the degradation of agricultural environmental hazard chemicals by microbes, specifically microbial consortia, under controlled conditions. A systematic literature review will be conducted using databases like ScienceDirect, Web of Science, and PubMed. Data extracted from selected studies will include information on pesticide types, microorganisms involved in degradation, mechanisms of microbial degradation, factors affecting microbial degradation, current trends in microbial degradation of pesticides, biodiversity of pesticide-degrading microbes, plasmid-borne pesticide resistance in bacterial communities, and strategies for pesticide degradation by microbial consortia. This research tries to present a comprehensive knowledge of microbial degradation mechanisms, highlight the potential of microbial consortia in pesticide degradation, and contribute to sustainable and eco-friendly approaches for addressing pesticide residues in agricultural environments. This study focused on the variables that affect the microbial breakdown of pesticides and the technique by which microorganisms decompose under natural conditions. In addition, the current trends of research on the microbial degradation of pesticides as well as a few visible challenges that continue to need attention were described.

Decision trees for determining the fate of laboratory animals.

Facilities involved in laboratory animal research often face ethical challenges such as: what should I do with the animals that are no longer suitable for experimental purposes? One of the common answers to this question is to kill them. And while numerous scientifically justifiable reasons exist for killing laboratory animals, we must not overlook our ethical responsibility towards these sentient beings. Animal facility managers and scientists frequently find themselves in a moral dilemma, torn between furthering their research and addressing the well-being of experimental animals required for their studies. We elaborated a concept consisting of six decision trees and recommendations for making informed decisions about the need to kill laboratory animals in research facilities, considering legal and ethical considerations. The concept is based on the German regulatory perspective. However, the measures and decisions for animal welfare can be implemented in all laboratory animal facilities. These recommendations suggest several courses of action, including implementing consistent breeding plans, exploring alternative uses, reassigning surplus animals and their organs, and establishing appropriate housing capacity limits that ensure species-appropriate care. We encourage scientists and animal facility managers to develop and implement decision-making frameworks and procedures tailored to their specific facilities, in the hope that this work will promote a thoughtful and responsible approach to the complex challenges associated with the killing of laboratory animals, advancing scientific progress and the humane treatment of these animals.

7503 Light Composition Alters Feline Stress in a Shelter Environment

Abstract Disclosure: A.M. Yaw: None. M.E. Gardella: None. J. Jacobs: None. H.M. Hoffmann: None. Millions of cats enter animal shelters each year. Unfortunately, due to high numbers of cats entering shelters and limited physical housing space, over 50% of healthy and adoptable cats with extended shelter stays are euthanized yearly. While animal shelters are essential for keeping cats safe from unfit living conditions, they are a stressful environment. High stress in cats increases health risks and undesirable behaviors and is associated with reduced adoptions. Given the role of light in regulating hormonal stress responses and behavior, we partnered with a Midwestern animal control and shelter to evaluate how light intensity and light composition impact feline behavior and urinary cortisol levels in 123 domestic cats (Felis catus; 65 male and 58 female). The impact of three lighting conditions, white, dim, and orange (blue-depleted) light, were evaluated for the first 5-6 days following admittance into the shelter. Individually housed adults (8 months-10 years) were outfitted with PetPace Smart Collars to evaluate locomotor activity, and cat stress was evaluated daily through urine samples and a behavioral approach test. All data were analyzed by experimenters who were blind to the groups and tested via one or two-way ANOVA or repeated measures mixed-effects model with Geisser-Greenhouse correction. Urinary cortisol levels were dependent on both light quality and day in shelter, where cats under orange light had reduced cortisol on days 4-5 in the shelter compared to cats in standard lighting. Light quality also altered cat hiding behavior, such that the percentage of cats hiding was increased on dim light compared to both standard and orange light. A novel behavioral approach task found reduced cat stress on days 4-5 in standard and orange light; however, dim light increased cat stress on day 5 in shelter. All cats exhibited circadian rhythms in locomotor activity with periods ranging from 21.33-35.67h independent of light condition and day in shelter, and locomotor activity increased on days 3-4 compared to day 2 in the shelter, with no effect of light condition. There were no sex differences in in hiding, PetPace activity, or cat stress scores under any light condition; however, females displayed increased urinary cortisol levels compared to males in standard, but not dim or orange, light. This shows for the first time that room light manipulation can help shelters enact easy and inexpensive changes that make the adjustment period shorter and less stressful for incoming cats which will improve animal wellbeing. Presentation: 6/1/2024

Situational Analysis of Cat Ownership and Cat Caring Behaviors in a Community with High Shelter Admissions of Cats.

Managing stray cats in urban areas is an ongoing challenge, and in Australia, many are euthanized. Most stray cats are from disadvantaged areas and are under 1 year of age. The Australian Pet Welfare Foundation intended to assess the impact of a free cat sterilization program in an area with high shelter intake of cats in the city of Ipswich, Queensland. The aims of this pre-intervention study were to undertake a situational analysis of cat ownership, semi-ownership and cat caring behaviours, and compare those in the same demographic with dog ownership and caring behaviours relating to sterilization rates, to provide a basis against which to assess the program's effectiveness. In a sample of 343 participants from that area, 35% owned cats and 3% fed stray cats. Cats were predominantly obtained from family or friends (31%) and shelters (20%). More respondents owned dogs (53%), which were most often sourced from breeders (36%) and family acquaintances (24%). More owned cats than owned dogs were sterilized (91% versus 78%). However, only 74% of cats aged 4 to <12 months were sterilized. Cat containment practices varied, with 51% of owners containing their cat(s) at all times, and a further 18% doing so at night. These results suggest the need for community-based programs that focus on sterilizing owned and semi-owned cats, and assisting semi-owners in becoming full owners to reduce stray cat populations and associated negative impacts. This includes assistance with cat containment where vulnerable native species are present. Public understanding of the causes and effective solutions for free-roaming cats, alongside legislative changes, are required to facilitate these efforts. Assistive programs aligned with One Welfare principles are expected to benefit the wellbeing of animals, humans and their environments.