Animals In India Research Articles

Legal Complications on Patenting Crossbreed Animals: A Comparative Discussion on India and Abroad

The patentability of cross-breed animals is a complex area facing constant evolution. The intellectual property law varies significantly in this domain between various jurisdictions. The study focuses mainly on a comparative analysis between IPR rights of India and abroad, where. This paper aims to provide a comparative discussion on the legal frameworks that govern rights of patentability of cross-bred animals in India and abroad, focusing on the lacunas that are highlighted to understand where the Indian legislation, despite being quite robust in plant cloning and breeding, falls short in animal cross breeding. The paper takes a dive into the international standards and practices and then tallies it in the Indian context. All the major and key jurisdictions such as the United States, European Union, and Australia are taken into consideration for the purpose of discussion, to understand what remedies could be generated on the basis of the comparative analysis. The Indian legislation, precedents and policies are considered to draw conclusions. It also tries to pass remarks on the implications on innovation, animal welfare, and efforts for global harmonization in IPR. To draft this paper, the authors have taken help from various primary and secondary resources.

The Occurrence of Colistin Resistance in Potential Lactic Acid Bacteria of Food-Producing Animals in India.

The overuse of colistin, the last-resort antibiotic, has led to the emergence of colistin-resistant bacteria, which is a major concern. Lactic acid bacteria which are generally regarded as safe are known to be reservoirs of antibiotic resistance that possibly pose a threat to human and animal health. Therefore, this study assessed the prevalence of colistin antimicrobial resistance in livestock in India, that is lactic acid bacteria in healthy chickens, sheep, beef, and swine of Mysore. Diverse phenotypic and genotypic colistin resistance were examined among the lactic acid bacterial species (n = 84) isolated from chicken (n = 44), sheep (n = 16), beef (n = 14), and swine (n = 10). Hi-comb, double-disk diffusion tests, Minimum Inhibitory Concentration (MIC), and biofilm formation were assessed for phenotypic colistin resistance. Specific primers for colistin-resistant genes were used for the determination of genotypic colistin resistance. Around 20%, 18%, and 1% were colistin-resistant Lactobacillus, Enterococcus, and Pediococcus species, respectively. Among these, 66.67% exhibited MDR phenotypes, including colistin antibiotic. The identified resistant isolates are Levilactobacillus brevis LBA and LBB (2), Limosilactobacillus fermentum LBF (1), and Pediococcus acidilactici CHBI (1). The mcr-1 and mcr-3 genes were detected in Levilactobacillus brevis LBA, LBB, and Pediococcus acidilactici CHBI isolated from chicken and sheep intestines respectively. The study identified colistin resistance determinants in lactobacilli from food animals, emphasizing the need for enhanced surveillance and monitoring of resistance spread. These findings underscore colistin resistance as a significant medical concern and should be integrated into India's ongoing antimicrobial resistance monitoring programs.

Tuberculosis in wild animals in India.

India is renowned for its complex megadiverse ecosystems and abundant biodiversity. Bovine tuberculosis (bTB) often remains synonymous with Mycobacterium bovis infection in cattle. The domain of tuberculosis (TB) among wild animals, induced by members of the Mycobacterium tuberculosis complex organisms (MTBC), is often underexplored and underreported in India. Within this context, instances of wild animal tuberculosis (wTB) have manifested across both captive and free-roaming animals. The sources contributing to wTB in animals can be human, animal, or environmental factors, thus illuminating the complex transmission pathways. The diagnosis of wTB continues to pose a formidable challenge, a consequence of the expansive taxonomic diversity in both the host and the pathogen. Complications inherent in acquiring samples from wildlife, the absence of standardized diagnostic protocols, limited insights into infection prevalence, and resource constraints compound diagnosis. Amidst these, adopting the comprehensive One Health paradigm surfaces as an imperative, accentuating the interconnectedness bridging human, animal, and environmental health. Recognizing key stakeholders and fostering intersectoral collaboration to provide enhanced diagnostic techniques driven by skilled personnel and advanced infrastructure play pivotal roles in a comprehensive strategy. Additionally, leveraging vaccination efforts contributes to effective control. A national wTB surveillance program is a cornerstone, ensuring an integrated and holistic approach to disease management. Through this review, we delve into the current landscape of wTB in India, unveiling its multifaceted challenges, and further explore the multifarious strategies that the One Health approach proffers in this dynamic endeavor.

Trade of Endangered Animals in India and its Critical Analysis

Trade of Endangered Animals in India and its Critical Analysis

Overpopulation of Animals in India: Analyzing Legislative Shortcomings

The overpopulation of animals in India is a critical issue with far-reaching consequences, impacting animal welfare, public health, and community safety. This legal research paper examines the root causes of this problem and the inadequacies in India's current legislative framework governing animal welfare. It explores how vague language, lax enforcement, and gaps in coverage have contributed to the unchecked growth of stray animal populations, resulting in suffering, public health risks, and safety concerns. Comparative analysis with regions known for effective animal welfare legislation reveals best practices and strategies that can be adapted to the Indian context. The paper proposes a series of legal reforms tailored to the Indian context, including mandatory spaying and neutering, comprehensive registration and microchipping, stricter licensing and breeding regulations, public education and awareness campaigns, strengthened animal cruelty penalties, and the introduction of Trap-Neuter-Return (TNR) programs for feral animals. Addressing challenges, objections, and ethical considerations, these recommendations aim to strike a balance between personal freedoms and the need for responsible pet ownership. Through case studies and a contextualized approach, this research underscores the importance of addressing legislative shortcomings in India to safeguard animal welfare, protect public health, and ensure community safety. The proposed reforms seek to harmonize India's rich cultural diversity with modern animal welfare standards, ultimately creating a more compassionate and secure environment for both animals and humans in the Indian context.

Ghost fishing gear threatening aquatic biodiversity in India

For decades, wildlife has been observed getting entangled in and affected by plastic litter. Despite the increasing annual accumulation of plastic waste in the Indian environment and its potential threat to marine biodiversity, the specific impacts of this litter often go unmeasured in India. Based on a search of social media (Facebook, Twitter, YouTube and Google), we evaluated the negative impacts of abandoned, lost or otherwise discarded fishing gear (ALDFG) on Indian biodiversity. Among the cases posted detailing interaction with anthropogenic litter, 35 species were affected with 144 individuals reported. Of the species entangled in this study, 13 are listed as vulnerable, endangered or critically endangered according to the IUCN. Sea turtles, fish and marine mammals were the most reported groups entangled in ALDFG. We also identified birds, land mammals (the Asian elephant), reptiles and invertebrates (crustaceans and sponges). These findings reveal that the majority of entangling litter impacting animals in India is from fishing activity. Five types of materials (fishing nets, rope, monofilament lines, trap and fish hook) accounted for the most (98 %) of entanglements. This study shows that ALDFG are not only a problem for marine species, but also for freshwater species. The presence of ALDFG in aquatic and marine environments contributes to the extinction risk of wildlife in India, which are already threatened by other human activities. There is therefore an urgent necessity to continue building management programs and develop government policies which attempt to protect the aquatic environment from ALDFG.

"Balancing Innovation and Ethics: The Debate on Patenting Plants and Animals in India"

"Balancing Innovation and Ethics: The Debate on Patenting Plants and Animals in India"

Cancer in Domestic Animals in India: Understanding Prevalence, Causes, and Treatment

Cancer in Domestic Animals in India: Understanding Prevalence, Causes, and Treatment

Overpopulation of Animals in India: Addressing Legal Shortcomings

Overpopulation of Animals in India: Addressing Legal Shortcomings

Gross and histopathological features of tuberculosis in cattle, buffalo and spotted deer (Axis axis) caused by Mycobacterium orygis

Mycobacterium orygis has been isolated from several cases of tuberculosis in various species of animal in India but documentation of the histopathological lesions caused by this organism is scant. Lung and liver tissues with caseous nodules from cattle (n = 8), lung samples from spotted deer (Axis axis) (n = 5) and lung and mediastinal lymph node samples from buffalo (n = 9) were subjected to histopathology and isolation of Mycobacterium spp. Isolation was carried out using the BACTEC MGIT 960 Automated Mycobacterial Detection System and acid-fast positive cultures were identified to species level using polymerase chain reaction (PCR) employing published primer pairs. Three M. orygis isolates (two from cattle, one from deer) were obtained, whole genome sequenced and the sequences submitted to the National Center for Biotechnology Information Sequence Read Archive. Eight samples (four cattle, one deer and three buffalo) were confirmed as M. orygis positive by PCR. Histopathological examination of the M. orygis-PCR-positive cattle samples revealed acid-fast organisms in lung sections along with macrophages, epithelioid cells, lymphocytes and Langhans giant cells. Granuloma stages I to IV were seen in the cattle and buffalo samples and stage III in the spotted deer sample. This report is the first description of the gross and histopathological lesions of tuberculosis caused by M. orygis in buffalo and documents the gross and histopathological findings of M. orygis tuberculosis in cattle and deer.

Study on occurrence of agrochemical poisoning in Bovines at veterinary clinics of Udgir

In view of unavailability of data on agrochemical poisoning in livestock animals in India, the present work was conducted with objective to study the epidemiological aspects of agrochemical poisoning in bovines over a period of five years. A total of 98 cases of pesticide poisoning in bovines have been reported during 2016-2020 at Veterinary Clinical Complex, College of Veterinary and Animal Sciences, Udgir with hospital prevalence of 0.39%. Among various agrochemicals, insecticide poisoning was responsible for 45.91% cases, fungicide for 17.34%, herbicides for 16.32%, unknown pesticides for 10.20%, fertilizers for 7.14% and rodenticides for 3.06% cases of poisoning in bovines. Year-wise higher number of cases were reported during 2016 (30.61%) followed by 2017, 2019 (19.38% each) and 2018 (18.36%) while, month- wise highest number of poisoning cases were reported in months of November (14.28%), December (13.26%), July and September, October (11.22% each). Season-wise highest occurrence of poisoning was observed during monsoon and post-monsoon seasons while least occurrence was observed during summer season. Age-wise highest occurrence of agrochemical poisoning was reported in 4-6 year and 0-2 year age-group animals. Species-wise occurrence of poisoning revealed highest occurrence in cattle (65.31%) than buffaloes (34.69%). Among cattle 67.18% male animals while 32.82% female animals were affected. All buffaloes confirmed for poisoning were female. The findings of the present study will be helpful for formulating strategies to reduce exposure of livestock animals to agrochemicals.

Covid-19 and the Plight of Animals in India: Safety And Prevention Approaches

No abstract

Seasonal, physiological and bacteriological risk factors for subclinical mastitis in dairy cows maintained under different farming conditions.

Subclinical mastitis (SCM) is a major health problem of dairy animals in India and across the globe. An identification of potential risk factors of SCM can help for efficient udder health management in dairy animals. In this study, apparently healthy cows (HF crossbred: n = 45; Deoni: n = 43) were screened for SCM during different seasons through milk somatic cell count (SCC: reference test using 200 × 103 cells/ml as cut off value), California mastitis test (CMT) and differential electrical conductivity (DEC) test at an organized research farm. SCM positive milk samples (n = 34) were inoculated in selective media for Coliform sp., Streptococcus sp. and Staphylococcus sp. and DNA was isolated (n = 10) for species confirmation by 16s rRNA method. Both bivariate and multivariate models were used for risk assessment. We found the cumulative prevalence of 31 and 65% SCM in Deoni and crossbred cows, respectively. Screening of 328 crossbred cows under field conditions revealed point prevalence of 55% SCM. Multivariate analysis revealed stage of lactation (SOL), milk yield in previous lactation and test day milk yield in Deoni cows, as well as parity and mastitis treatment history in current lactation in HF crossbred cows as risk factors. SOL was a significant factor under field conditions. Receiver operated characteristic curve analysis revealed better accuracy of CMT than DEC. We found more mixed infections due to Staphylococcus sp. and Streptococcus sp. in culture, while 16s rRNA based molecular method revealed lesser-known pathogens associated with SCM. It is concluded that SCM prevalence rate is higher in crossbred than indigenous cows and these breeds have different risk factors for SCM. HF crossbred cows had similar SCM prevalence rate under different farming conditions, where CMT can be used for SCM diagnosis with excellent accuracy. The 16s rRNA method is useful for specific identification of lesser known and emerging mastitis pathogens.

Docking analysis of circulating CTX-M variants in multi-drug resistant, beta-lactamase and biofilm-producing E. coli isolated from pet animals and backyard livestock.

The generation of antimicrobial-resistant bacteria largely depends on the use of antimicrobials not only in humans but also in pet animals and livestock. The present study was conducted to detect the occurrence of beta-lactamase and biofilm-producing- E.coli in healthy pet and backyard livestock. The study also intended on molecular docking experiments to confirm the nature of the catalytic mechanism in β-lactamase enzymes, encoded by the various blaCTX-M genotypes and phylogenetic analysis to reveal clonal relationship of the animal origin E. coli isolates with human clinical strains. The rectal swabs were collected from healthy dogs (n=254), cats (n=108), sheep (n=119) and goats (n=143) in India. In total 247 (76.47%) E. coli strains were identified as ESBL producers. The possession of ESBL-producers was significantly more (p<0.05) in pets than in the backyard livestock. Most of the strains possessed blaCTX-M-15 like clones. E. coli strains possessing blaCTX-M-15.2, blaCTX-M-157, blaCTX-M-181 and blaCTX-M-218 like clones, isolated from pets were not reported earlier. The study detected 56.65% of E. coli strains as moderate or strong biofilm producers possessing biofilm-associated genes (csgA, rcsA, rpoS, sdiA). ESBL-producing E. coli showed phenotypical resistance to tetracycline (93.1%), azithromycin (89.8%), ampicillin (84.2%), cefotaxime (80.9%), doxycycline (82.5%), co-trimoxazole (80.9%), ampicillin/cloxacillin (76.9%). The CTX-M variants obtained in this study were modelled by the SWISS-MODEL and verified. Ligand having minimum binding energy, show the highest affinity of β-lactamases for cefotaxime and cefpodoxime. The Gibbs free energy release for all 14 different complex ranges between -6.9 (CTX-M-15.2+cefpodoxime) to -5.3 (CTX-M-218+cefpodoxime) Kcal/mol. Phylogenetic analysis of the animal origin ESBL-E. coli strains revealed a partial clonal relationship with the clinical isolates of local human patients. The present study described the significant presence of biofilm and β-lactamase producing, multi-drug resistant E. coli in pet animals having public health importance.

Preliminary survey on the Indian wild boar damage in agricultural lands using ITKs in adjoining Tiger reserve of Tamil Nadu, India

The Indian Wild Boar (Sus scrofa cristatus L.) is a widely distributed animal in India. The incidence of Indian Wild Boar (Sus scrofa) and its harm to agricultural crops were investigated in a preliminary survey. The survey included the major agricultural crops that were damaged by the wild boar and the preventive measures that the farmers have taken to protect the crop from the raiding of wild boar. The study was taken up from the months of December to February (2021-2022) in and around three Tiger Reserves (Mudumalai, Anamalai and Sathyamangalam) with the help of Tamil Nadu Forest Department. With this outline, the purpose of this study is to highlight and record in detail about the tribal communities traditional knowledge on the plants that are not preferred by the wild boar. The survey was conducted in tribal hamlets encompassing the tiger reserves. Through the semi-structured informal interviews, Questionnaire, Interactions during on farm fields, the study has recorded that the wild boar has an aversion to eight plant species and eight traditional methods (five physical method and three biological method) were identified.

Use of sexed semen for genetic improvement of indigenous dairy cattle and buffaloes productivity

The productivity of dairy animals in India needs to be improved to feed the growing population in the decadesahead. Sperm sexing offers a promising breeding strategy to meet the increased demand for food production andnutritional protection. Application of sexed sperm in the breeding of indigenous cattle and buffalo would helpto protect endangered species, and it also helps to improve the productivity of indigenous milch animals whichcan survive long starvation and are well adopted to Indian climatic conditions along with disease resistance. Theproductivity of crossbreed milch animals is also required to meet the increasing food demand associated withpopulation growth. This review discusses the sustainability of Indian dairy animals and provides sperm sexingstrategies to boost dairy animal production in India.

Management Of Heat Stress in Farm Animals in India

Management Of Heat Stress in Farm Animals in India

SOLAR OPERATED FARM PROTECTION DEVICE

Crops in farms are many times ravaged by local animals like buffaloes, cows, goats, birds, and fire etc. This leads to huge losses for the farmers. It is estimated that 30-40 percent of crops are destroyed annually due to attacks by wild animals in India. Elephants, pigs, boars and deer are the most common perpetrators of the destruction. This is a Automatic Battery charging system Using Solar cell. This system uses a light sensor(LDR) to activate alarm System during night to return the wild animals approaching near the field . In such a case the sensor signals (Signal from LDR) activates the alarm system and Lighting system made To scare the animals. The alarm also gives alert to the farmer to about animals if he is near to field. Therefore, the designed system is affordable and useful to the farmers. The designed system won't be harmful to animals and person , and it protects the farm areas. further implementation can be carried out according to the requirement of farm , Device can adjust and modified. COMPONNATS : Solar Panel, Battery, BC 547 Transistor, Resistor ,Diode (1N400), Light Dependent Resistor(LDR), Horn Speaker, PIR Sensors, Arduino UNO, LED, Buzzer.

Prevalence of Anaplasma marginale in Water Buffaloes (Bubalus bubalis) in the State of Uttar Pradesh, North India

Background: Bovine anaplasmosis caused by Anaplasma marginale is highly endemic in tropical and sub-tropical regions of the world where it accounts for huge economic losses to the livestock sector. Approximately 30% of water buffalo (Bubalus bubalis) herds in India are located in Uttar Pradesh (U.P). However in India, most of the research studies on Anaplasma are focused on cattle whereas studies on this pathogen in water buffaloes have been neglected. Therefore, present investigation was undertaken for determining the prevalence of A. marginale infection in water buffaloes in U.P, North India. Methods: In the current investigation during 2017-2019, the prevalence of A. marginale infection in 228 water buffaloes in three districts of North-West U.P was studied by microscopy and by nested-PCR amplification of major surface protein 5 (msp5) gene. Result: Microscopic examination of blood smears of 228 water buffaloes revealed 7.89% animals positive for A. marginale. Conventional PCR of full length major surface protein 5 (msp5) gene detected 29.39% and nested-PCR amplification of this gene detected 64.0% of these animals as positive for A. marginale infection. A high incidence of the infection detected in this study area suggests that water buffaloes may serve as reservoir hosts of A. marginale and play a vital role in the spread of infection to other susceptible animals. However further studies are required on larger buffalo populations for determining the epidemiological status of anaplasmosis in these animals in India.

Effect of bypass fatty acid and Tinospora cordifolia supplementation on production performance and milk fatty acid profiling in Murrah buffaloes (Bubalus bubalis).

Buffaloes contribute about 49% of total milk production and are considered as major milk-producing animals in India. During the early stage of lactation, mostly high producing animals suffer from negative energy balance (NEB) due to imbalance between the needs and availability of energy, and this NEB has a negative impact on production and health of buffaloes. Taking this into consideration, a study was conducted at National Dairy Research Institute, Karnal on 20 freshly calved healthy Murrah buffaloes (Bubalus bubalis). Buffaloes were grouped depending on their previous milk yield, body weight, and parity as T0 (control), T1 (fatty acids), T2 (Tinospora), and T3 (mix) groups having five animals each. Supplementation of 150g of bypass fatty acids in T1 group, 150g of Tinospora powder in T2 group, and combination of 150 of bypass fatty acid and 150g of Tinospora powder in T3 group over and above the standard feeding schedule was done for a duration of 90days whereas standard feeding schedule was followed in T0 group. A significant (P < 0.05) increase in average daily milk yield was observed in T1 (10.06 ± 0.56kg/d/h), T2 (10.30 ± 0.53kg/d/h), and T3 (10.11 ± 0.48kg/d/h) in comparison to control group (8.05 ± 0.38kg/d/h). Significantly (P < 0.05) higher milk fat percent and total solids were also recorded in treatment groups over the control group. Other milk constituents (SNF, protein, lactose, ash) and milk properties (pH, conductivity, freezing point, density) remained unaffected by the supplementations. The somatic cell count values were significantly (P < 0.05) decreased in T2 and T3 with the advancement in the lactation in comparison to T0 and T1. Supplementation also leads to significantly (P < 0.05) higher MUFA and PUFA contents in milk of treatment group buffaloes in comparison to control group. The study outcomes state that combination of bypass fatty acids and Tinospora cordifolia supplementation improves milk production and its composition in lactating Murrah buffaloes.