Different Groups Of Animals Research Articles

Snakes as sentinel of zoonotic yeasts and bio-indicators of environmental quality.

Reptiles in the wild or as pets may act as spreaders of bacteria, viruses, fungi and parasites. However, studies on the mycobiota of these animals are scanty. This study investigates the occurrence of yeasts from the cloacal swabs of snakes of different origins and the antifungal profile of the isolated strains. A total of 180 cloacal samples of snakes were collected from Morocco (Group I: n = 68) and Italy (Group II: n = 112). Yeast species were biochemically and molecularly identified. A total of 72 yeast strains belonging to 13 genera, 8 from snakes in Group I and five from snakes in Group II were identified. The most frequently isolated species were Trichosporon asahii (22.2%) and Candida tropicalis (15.3%) from snakes in Group I and Debaryomyces spp. (16.7%) and Metahyphopichia silvanorum (11.1%) from snakes in Group II. Multiple azole and amphotericin B (AmB) resistance phenomena were detected among isolated yeasts. Azole multi drug resistance phenomena were detected among yeasts from Group I and Rhodotorula mucilaginosa from Group II, whereas AmB resistance phenomena among those from Group II. Data suggest that snakes may harbor pathogenetic yeasts, being potential reservoirs and spreaders of these organisms in the environment. Since the yeast species community from different groups of animals as well as their antifungal profile reflects the epidemiology of human yeast infections in the same geographical areas, the results indicate that snakes may be considered as sentinels for human/animal pathogenic microorganisms and bio-indicators of environmental quality.

Effect of genetically engineered drugs in aseptic necrosis of the femoral head in rats

Bone tissue is a dynamic structure with a metabolic function. The maintenance of bone homeostasis is carried out due to the continuous process of its renewal, remodeling. At the same time, a number of pathological processes, such as ischemic catastrophe, can lead to a violation of the balance of maintaining the constancy of the bone structure. One of these diseases is aseptic necrosis of the femoral head. The presented study analyzes the dynamics of expression of genes involved in maintaining bone tissue homeostasis, changes in the histological picture during the development of aseptic necrosis of the femoral head in laboratory rats that did not receive genetically engineered drugs and against the background of the use of inhibitors of biological action IL-6, TNF-α. After induction of aseptic necrosis in the proximal epiphysis of the femur, the histological picture in animals of different groups was not the same. More preserved bone architectonics and a larger volume of bone plates were recorded in rats receiving genetically engineered drugs compared to animals without the introduction of biological agents. The latter also had the most vivid picture of osteodestruction with increased expression of proinflammatory cytokine genes. In animals, against the background of the use of drugs of inhibitors of the biological action of IL-6, TNF-α, from the second week after induction of aseptic necrosis of the mRNA, the profile of the spongiose bone of the proximal epiphysis of the femur tended to increase the expression of osteoreparation genes. At the same time, the greatest inhibition of osteoclastogenesis gene expression was obtained in rats after injection of a monoclonal antibody to the IL-6 receptor.

Anthropozoonotic parasitoses of dogs and cats in the urban ecosystem of Vladivostok, Russia

To investigate the role of domestic dogs and cats in the spread of parasitoses in the Vladivostok urban ecosystem. Biological materials from 782 dogs and 189 cats were used in the research, which were examined by methods of complete parasitological autopsy, microscopy and flotation of faecal samples using saturated solutions of zinc sulfate and sodium nitrate.Fourteen parasite taxa have been identified: nematodes (Nematoda) Ancylostoma caninum, Dirofilaria immitis, Toxascaris leonina, Toxocara canis, T. mystax, Uncinaria stenocephala, Taenia sp., T. hydatigena; tapeworms (Cestoda) Diphyllobothrium latum, Dipylidium caninum, Hydatigera taeniaeformis; flukes (Trematoda) Metagonimus sp.; and parasitic alveolates (Conoidasida) Cystoisospora sp., C. felis. The paper presents the values of the prevalence of infection and statistical reliability of their differences for different groups of animals, depending on gender and age. Nematodes were the core taxa of the parasitofauna identified. Explanations for the results obtained are offered and possible sources of human infection are described. Despite the fact that parasitic‐faunal complexes in the ecosystem of a modern city are much less branched and represented by fewer species than in the wild, there remains a high risk of human infection with zoonotic parasites in urban biocenoses due to the high level of intensive population interactions. Therefore, there is a requirement of high alertness of supervisory authorities in relation to anthropozoonotic parasitoses in an urban environment.

LInear measurements of carcases of the Myrhorod breed pigs

Objective. To evaluate the dependence of carcass characteristics of pigs of the Myrhorod breed on the starting capabilities of animals in the neonatal period of development. Methods. Three groups of pigs were formed, differing among themselves in the number of piglets in the litter at birth. For the purpose of comparative assessment, indexes for three groups as a whole were also determined. Young boars were selectedfor fattening and further slaughter. All piglets were received in one period, had free access to feed in accordance with the needs of sex and age groups. This stage of research was conducted at the experimental base of Institute of Pig Breeding and AIP NAAS. The main measurements of pig carcasses were taken at the meat processing plant of "Prodfoodservice" LLC in Poltava. Animals with a live weight of 100 kg were selected for the assessment. General rules were followed for the assessment of pig carcasses. The topography of fat deposition on the carcass was determined by the following points of taking measurements of the thickness of lard with skin: at the withers; at the level of 6 - 7 thoracic vertebrae; on the waist; on the crosses in three points; at chest level. The formula proposed by N. V. Pivniak was used to determine the average thickness of lard along the spine (watering of the carcass). To compare animals of different groups of pigs of the Myrhorod breed, the full meatiness index (FMI) was calculated. The research results were processed using the traditional method of variational statistics. Results. The differences between the different groups were insignificant in relation to the girth of the sternum. According to the latitudinal parameters of the bacon half of the carcass, there were also no significant differences between different groups of pigs, with a slight shift of preference towards the third group. Regarding the ratio of the front to the back parts of the bacon halves, the smallest values were found in the animals of the second group, and the largest in the first group. Regarding the determination of fat thickness at different points of taking measurements, a clear picture regarding the superiority of a certain group was not determined, although according to most indexes, the carcasses of animals of the first group were marked by larger actual measurements. The animals of all three groups did not meet the requirements for the first extra and the second category of pigs for slaughter according to the fat thickness index at the level of 6 - 7 thoracic vertebrae. All evaluated carcasses were assigned to the third category, which corresponds to the type of productivity of Myrhorod breed of pigs. According to the comprehensive assessment of carcass watering, the highest values were obtainedfor the first group, the average values for the second group, and the smallest values for the third group, respectively. The opposite picture was observed for the full meatiness index, although differences in this index were also unlikely. By comparing the main and most important indexes of the linear diameters of the carcasses of three different groups with the same indexes for all evaluated animals, the animals of the third group were the closest to the average values in relation to the front to the back parts of the bacon halves, the thickness of the lard at the level of the 6 - 7th thoracic vertebrae. At the same time, the animals of the second group were the closest to the average values according to the full meatiness index. Conclusions. As a result of the conducted research, it was found that there is no dependence of meatiness indexes, quality characteristics ofpig carcasses, and in particular, their salinity, etc. from the starting capabilities of animals in the neonatal period of development. Non-compliance with the requirements for the first extra and second categories of pigs for slaughter was found, which indicates that the productivity of the new generation of animals of the Myrhorod breed still belongs to animals of universal and fatty genotypes, although the carcasses according to the lard thickness index are at the level of 6 - 7 thoracic vertebrae were at the lower limit of the third category of pigs for slaughter. Keywords: pigs, Myrhorod breed, slaughter qualities, pig carcasses, lard, meatiness, watering of carcasses.

LInear measurements of carcases of the Myrhorod breed pigs

Objective. To evaluate the dependence of carcass characteristics of pigs of the Myrhorod breed on the starting capabilities of animals in the neonatal period of development. Methods. Three groups of pigs were formed, differing among themselves in the number of piglets in the litter at birth. For the purpose of comparative assessment, indexes for three groups as a whole were also determined. Young boars were selectedfor fattening and further slaughter. All piglets were received in one period, had free access to feed in accordance with the needs of sex and age groups. This stage of research was conducted at the experimental base of Institute of Pig Breeding and AIP NAAS. The main measurements of pig carcasses were taken at the meat processing plant of "Prodfoodservice" LLC in Poltava. Animals with a live weight of 100 kg were selected for the assessment. General rules were followed for the assessment of pig carcasses. The topography of fat deposition on the carcass was determined by the following points of taking measurements of the thickness of lard with skin: at the withers; at the level of 6 - 7 thoracic vertebrae; on the waist; on the crosses in three points; at chest level. The formula proposed by N. V. Pivniak was used to determine the average thickness of lard along the spine (watering of the carcass). To compare animals of different groups of pigs of the Myrhorod breed, the full meatiness index (FMI) was calculated. The research results were processed using the traditional method of variational statistics. Results. The differences between the different groups were insignificant in relation to the girth of the sternum. According to the latitudinal parameters of the bacon half of the carcass, there were also no significant differences between different groups of pigs, with a slight shift of preference towards the third group. Regarding the ratio of the front to the back parts of the bacon halves, the smallest values were found in the animals of the second group, and the largest in the first group. Regarding the determination of fat thickness at different points of taking measurements, a clear picture regarding the superiority of a certain group was not determined, although according to most indexes, the carcasses of animals of the first group were marked by larger actual measurements. The animals of all three groups did not meet the requirements for the first extra and the second category of pigs for slaughter according to the fat thickness index at the level of 6 - 7 thoracic vertebrae. All evaluated carcasses were assigned to the third category, which corresponds to the type of productivity of Myrhorod breed of pigs. According to the comprehensive assessment of carcass watering, the highest values were obtainedfor the first group, the average values for the second group, and the smallest values for the third group, respectively. The opposite picture was observed for the full meatiness index, although differences in this index were also unlikely. By comparing the main and most important indexes of the linear diameters of the carcasses of three different groups with the same indexes for all evaluated animals, the animals of the third group were the closest to the average values in relation to the front to the back parts of the bacon halves, the thickness of the lard at the level of the 6 - 7th thoracic vertebrae. At the same time, the animals of the second group were the closest to the average values according to the full meatiness index. Conclusions. As a result of the conducted research, it was found that there is no dependence of meatiness indexes, quality characteristics ofpig carcasses, and in particular, their salinity, etc. from the starting capabilities of animals in the neonatal period of development. Non-compliance with the requirements for the first extra and second categories of pigs for slaughter was found, which indicates that the productivity of the new generation of animals of the Myrhorod breed still belongs to animals of universal and fatty genotypes, although the carcasses according to the lard thickness index are at the level of 6 - 7 thoracic vertebrae were at the lower limit of the third category of pigs for slaughter. Keywords: pigs, Myrhorod breed, slaughter qualities, pig carcasses, lard, meatiness, watering of carcasses.

Anti-manic effect of deep brain stimulation of the ventral tegmental area in an animal model of mania induced by methamphetamine.

Treatment of refractory bipolar disorder (BD) is extremely challenging. Deep brain stimulation (DBS) holds promise as an effective treatment intervention. However, we still understand very little about the mechanisms of DBS and its application on BD. The present study aimed to investigate the behavioural and neurochemical effects of ventral tegmental area (VTA) DBS in an animal model of mania induced by methamphetamine (m-amph). Wistar rats were given 14 days of m-amph injections, and on the last day, animals were submitted to 20 min of VTA DBS in two different patterns: intermittent low-frequency stimulation (LFS) or continuous high-frequency stimulation (HFS). Immediately after DBS, manic-like behaviour and nucleus accumbens (NAc) phasic dopamine (DA) release were evaluated in different groups of animals through open-field tests and fast-scan cyclic voltammetry. Levels of NAc dopaminergic markers were evaluated by immunohistochemistry. M-amph induced hyperlocomotion in the animals and both DBS parameters reversed this alteration. M-amph increased DA reuptake time post-sham compared to baseline levels, and both LFS and HFS were able to block this alteration. LFS was also able to reduce phasic DA release when compared to baseline. LFS was able to increase dopamine transporter (DAT) expression in the NAc. These results demonstrate that both VTA LFS and HFS DBS exert anti-manic effects and modulation of DA dynamics in the NAc. More specifically the increase in DA reuptake driven by increased DAT expression may serve as a potential mechanism by which VTA DBS exerts its anti-manic effects.

Bright daylight produces negative effects on affective and cognitive outcomes in nocturnal rats

The daily light/dark cycle affects animals' learning, memory, and cognition. Exposure to insufficient daylight illumination negatively impacts emotion and cognition, leading to seasonal affective disorder characterized by depression, anxiety, low motivation, and cognitive impairment in diurnal animals. However, how this affects memory, learning, and cognition in nocturnal rodents is largely unknown. Here, we studied the effect of daytime light illuminance on memory, learning, cognition, and expression of mRNA levels in the hippocampus, thalamus, and cortex, the higher-order learning centers. Two experiments were performed. In experiment one, rats were exposed to 12 L:12D (12 h light and 12 h dark) with a 10, 100, or 1000 lx daytime light illuminance. After 30 days, various behavioral tests (novel object recognition test, hole board test, elevated plus maze test, radial arm maze, and passive avoidance test) were performed. In experiment 2, rats since birth were raised either under constant bright light (250 lx; LL) or a daily light-dark cycle (12 L:12D). After four months, behavioral tests (novel object recognition test, hole board test, elevated plus maze test, radial arm maze, passive avoidance test, Morris water maze, and Y-maze tests) were performed. At the end of experiments, rats were sampled, and mRNA expression of Brain-Derived Neurotrophic Factor (Bdnf), Tyrosine kinase (Trk), microRNA132 (miR132), Neurogranin (Ng), Growth Associated Protein 43 (Gap-43), cAMP Response Element-Binding Protein (Crebp), Glycogen synthase kinase-3β (Gsk3β), and Tumour necrosis factor-α (Tnf-α) were measured in the hippocampus, cortex, and thalamus of individual rats. Our results show that exposure to bright daylight (100 and 1000 lx; experiment 1) or constant light (experiment 2) compromises memory, learning, and cognition. Suppressed expression levels of these mRNA were also observed in the hypothalamus, cortex, and thalamus. These results suggest that light affects differently to different groups of animals.

Antidiabetic and Antioxidant Effect of Ethanolic Extract of Propolis from Meiganga (Cameroon) on Type 2 Diabetes in Rats

Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia. In Cameroon, the traditional treatment of this pathology is based on the use of Propolis. The present study aims to evaluate the antidiabetic and antioxidant properties of the ethanoic extract of Meiganga propolis (Adamawa Region, Cameroon). To confirm the different properties of this extract, the glycaemia, lipid profile and oxidative stress parameters of different groups of animals were assessed in a type 2 diabetes model induced by the Hypercaloric Sucrose Diet combined with dexamethasone. Simultaneous administration of the ethanolic extract of Meiganga propolis (EEMP 300 mg/kg) and the hypercaloric sucrose diet to rats for 30 days prevented a significant increase in fasting blood glucose levels compared with animals in the diabetic control group, whose fasting blood glucose levels were 78.25 ± 2.29 and 156.5 ± 2.75 mg/dL respectively on day 30. In terms of lipid profile, the administration of propolis extract (300 mg/kg) prevented a significant increase in LDL-cholesterol and triglyceride levels compared with animals in the diabetic control groups. The values were 27±2.71 mg/dL and 97.8±2.92 mg/dL for LDL-cholesterol. In terms of oxidative stress, simultaneous administration of propolis extract (300 mg/kg) and a high calorie diet to rats for 30 days prevented a significant increase in malondialdehyde (MDA) and increased superoxide dismutase (SOD) levels compared with animals in the diabetic control group. Values for this superoxide dismutase in the liver were 81.72 U/g of organ for EEMP 300 mg/kg and 58.6 U/g of organ in the liver of diabetic rats. These results justify the use of ethanoic extract of Meiganga propolis in the prevention of type 2 diabetes in Cameroon.

Discussion of Josh Milburn’s Just Fodder: The Ethics of Feeding Animals

In Just Fodder: The Ethics of Feeding Animals, Josh Milburn thinks through the implications of feeding animals by focusing on the relationships between humans and three different groups of animals: (1) animal companions; (2) animal neighbours; and (3) wild animals. In my comments, I concentrate on how the actions and agency interests of these animals problematise some of Milburn’s assumptions and normative prescriptions. My overall aim is to show how giving animal agency more prominence in our thinking about what we owe to them has significant implications. It is my view that current theorising about other animals focuses too heavily on their experiential welfare and not enough on what animals do and the normative significance of what they do. Though I agree with Milburn that all animals have a right against being killed and made to suffer, a full ethical analysis of our relations with other animals must also consider their (equally weighty) interest in self-determination.

Balkhash-Alakol Basin animal world database creation process for the purpose of cadastre maintenance

This paper describes the database (DB) development process of the cadastre of the fauna of the Balkhash-Alakol Basin that will be used for analyzing the spatial and temporal dynamics of population status and the graphical display of spatial data. The data was organized using the methods of relational database organization (SQL) with sets of individual criteria adapted for different groups of animals (parasitofauna, malacofauna, arachnofauna, entomofauna, ichthyofauna, herpetofauna, ornithofauna, and theriofauna). This database considers several additional technical aspects of its further integration with other databases, for example, geographical, climatic, etc., which allows for a deep analysis of the relationship between various environmental factors and population dynamics. For the functioning of the user interface, server architecture, and program interface, an application on the Django framework was developed, which simplified the processes of software development and allowed specialists to flexibly adapt the database to the own needs and project goals while simultaneously increasing the autonomy, security, and stability of the database.

Сравнительная оценка мясной продуктивности молодняка овец при различных способах откорма

Purpose. Comparative analysis of meat productivity of rams of the Edilbaev breed during their grazing and fattening. Materials and Мethods. Scientific and production experience was carried out in the peasant farm Abdulkhalikov M.A. (Svetloyarsky district, Volgograd region) on two groups of rams of Edilbaev breed. The total number of animals participating in the experiment is 40. The formation of experimental groups was carried out according to the principle of analogues according to the methodology of A.I. Ovsyannikov (1976). The duration of the main experimental period was 120 days. The dynamics of live weight of rams was determined by measuring this indicator in each animal in the morning, before feeding, at the age of 4, 6 and 8 months. The rams were slaughtered (3 heads from each group) when the animals reached the age of 8 months. When studying meat productivity, we took into account: the weight of animals after fasting, the weight of the chilled carcass, the yield of the carcass, the weight and yield of internal fat, the fat tail weight and the yield of fat tail, slaughter weight, slaughter yield. All digital material obtained during the research was processed using statistical methods and Microsoft Excel software and determination Student-Fisher's reliability criterion of the difference. Results. Data on the dynamics of live weight at the age of 8 months indicate that the average live weight of fattening rams was 55.20 kg versus 50.80 kg for their peers in grazing. The difference in favor of fattening rams was 4.40 kg or 8.66% and was significant. Their superiority in absolute and average daily growth was 3.70 kg and 30.8 g, respectively. Over a period of 4 to 8 months, i.e. over 120 days of research, the average daily gain of fattening rams was 185.0 g versus 154.2 g for grazing rams of the same age. Fattening animals were superior to their peers in other slaughter indicators: in carcass weight - by 1.96 kg or 3.6% (P≤0.01), in internal fat weight - by 0.20 kg or 17.24 % (P≤0.01), in fat tail weight – by 0.60 kg or 18.30% (P≤0.05), by slaughter weight – by 3.93 kg or 14.41% (P≤0.01). At the same time, the slaughter yield for fattening rams was 58.10%, which is 1.90% higher than for grazing animals. According to morphological indicators, fattening rams are superior to their peers in fattening in terms of pulp weight by 2.08 kg or 12.17%. There was no significant difference in the mass of bone and connective tissue between animals of different groups. According to the meatiness index, grazing rams were inferior to their peers by 0.43 absolute values. Conclusion. Intensive fattening of young sheep of Edilbayev breed is a more effective method of fattening compared to grazing. The level of profitability of mutton production for fattening rams was 36.16% versus 13.47% for grazing rams of the same age. The difference in favor of fattening rams was 22.69%.

Estrogen receptor β exerts neuroprotective effects by fine-tuning mitochondrial homeostasis through NRF1/PGC-1α

BackgroundEstrogen deficiency causes mitochondrial defects that precede pathological changes related to Alzheimer's disease (AD) in the mouse model of postmenopause. The aim of this study was to investigate in such a mouse model whether and how estrogen receptor β (ERβ) was involved in prevention of mitochondrial damage and protection of neurons in the hippocampus. MethodsA mouse model of postmenopausal AD was created by ovariectomizing female 3xTg-AD mice, some of which were subcutaneously injected for six weeks with the non-steroidal ERβ agonist diarylpropionitrile. ERβ expression in female C57BL/6J mice was knocked down using shRNA interference. The different groups of animals were compared in terms of cognitive function using the Y-maze test, new object recognition test, and Morris water maze test, expression of numerous proteins related to mitochondrial biogenesis, mitophagy, apoptosis, and mitochondrial membrane potential, as well as deposition of amyloid β and neurofibrillary tangles. To complement these in vivo studies, we probed the effects of diarylpropionitrile on ERβ expression, apoptosis, and mitochondrial homeostasis in primary rat hippocampal neurons treated with amyloid β. ResultsERβ knockdown in C57BL/6J mice produced cognitive impairment, reduced mitochondrial biogenesis by downregulating PGC-1α, NRF1, mtTFA, and TOM20, and decreased mitophagy by downregulating Pink1, Parkin, and LC3B while upregulating PARIS and p62. ERβ knockdown promoted neuronal apoptosis by upregulating Cleaved-Caspase 9, Cleaved-Caspase 3, and Bax, while downregulating Bcl2 in hippocampus. Diarylpropionitrile mitigated cognitive decline in ovariectomized 3xTg-AD mice, which was associated with downregulation of BACE1, reduction of Aβ deposition, neurofibrillary tangles, and tau hyperphosphorylation, and upregulation of ERβ, increases in mitochondrial biogenesis and mitophagy, and decreases in apoptosis. The effects of diarylpropionitrile in mice were recapitulated in Aβ-injured primary rat hippocampal neurons. ConclusionsERβ activation can support learning and memory and alleviate AD symptoms in the postmenopausal AD model, which may involve regulation of neuronal mitochondrial biogenesis and mitophagy via NRF1/PGC-1α. This study supports further research on ERβ as a therapeutic target for postmenopausal women with AD.

Whole genome sequence-based analysis of Staphylococcus aureus isolated from bovine mastitis in Thuringia, Germany.

Bovine mastitis is a common disease of dairy cattle causing major economic losses due to reduced yield and poor quality of milk worldwide. The current investigation aimed to gain insight into the genetic diversity, antimicrobial resistance profiles and virulence associated factors of Staphylococcus (S.) aureus isolated from clinical bovine mastitis in dairy farms in Thuringia, Germany. Forty Staphylococcus aureus isolates collected from clinical bovine mastitis cases from 17 Thuringian dairy farms were phenotyped and genetically characterized using whole genome sequencing. Out of 40 S. aureus, 30 (75%) were confirmed as methicillin resistant isolates. The isolates showed elevated antimicrobial resistance against penicillin, tetracycline and oxacillin, i.e., 77.5, 77.5, and 75%, respectively. Lower resistance rates were found against moxifloxacin, ciprofloxacin, gentamicin and trimethoprim/sulfamethoxazole, i.e., 35, 35, 30, and 22.5%, respectively. While resistance against clindamycin and erythromycin was rarely found (5 and 2.5%, respectively). All isolates were susceptible to linezolid, teicoplanin, vancomycin, tigecycline, fosfomycin, fusidic acid and rifampicin. These isolates were further allocated into five different sequence types: ST398 (n = 31), ST1074 (n = 4), ST504 (n = 3), ST582 (CC15) (n = 1) and ST479 (n = 1). These isolates were also assigned to seven clusters with up to 100 SNP which has facilitated geographical mapping and epidemiological distribution in Thuringia. Strains belonging to ST398 were classified into clusters 1, 2, 3, 4 and 7. The isolates of ST504 were of cluster 5, those of ST1074 were belonging to cluster 6. Resistance genes blaZ, blaI and blaR associated with penicillin resistance were found in 32 (80%) strains, all except one were belonging to ST398. Methicillin resistance associated mecA was identified in 30 (96.8%) isolates of ST398. All tetracycline and erythromycin resistant isolates were of ST398, and all harbored both tetM and ermA. About 90.3% of tetracycline resistant isolates assigned to ST398 were also carrying tetK gene. The point mutations parC_S80F, gyrA_S84L and parC_S80Y in gyrA and parC associated with quinolone resistance were found in all phenotypically resistant isolates to ciprofloxacin and moxifloxacin (n = 14). Sixty-eight virulence genes were identified among isolates. Both lukD/E and lukM/F-PV-P83 were identified in 22.5% of isolates, all were non-ST398. In this study, ST398 had the highest potential to cause disease and had a massive prevalence in bovine mastitis cases. Five different sequence types and seven clusters were identified in the federal state of Thuringia. The circulation of some clusters in the same region over several years shows the persistence of cluster-associated infection despite the intensive veterinary care. On the other hand, some regions had different clusters at the same year or in different consecutive years. Different sequence types and associated different clusters of S. aureus were geographically widely distributed among dairy farms in Thuringia. The findings of this study show that various clusters have the potential to spread over a large geographical scale. The detection of LA-MRSA on dairy farms, which is known for cabapility to widely spread among different groups of animals, humans and their environment urges for the implementation of national wide strategic programs. The identification of CA-MRSA among the isolates such as ST398 poses a significant risk for the transmission of such strains between animals and humans on dairy farms.

Ruscogenin timing administration mitigates cerebral ischemia-reperfusion injury through regulating circadian genes and activating Nrf2 pathway

BackgroundRuscogenin (Rus), a steroidal sapogenin extracted from Ophiopogon japonicus (L. f.) Ker-Gawl., has the effect of alleviating cerebral ischemia-reperfusion injury (IRI), acute lung injury. At present, the chronopharmacological effects of Rus are still unknown. PurposeThis study explored the alleviating effect and mechanism of Rus timing administration on mice cerebral IRI. MethodsThe animals in different groups were administrated Rus (10 mg/kg) by gavage at four time points (23:00–01:00, 05:00–07:00, 11:00–13:00, 17:00–19:00) respectively for 3 days. On the 4th day, middle cerebral artery occlusion (MCAO) surgery was operated during 5:00–7:00. Behavioral tests were executed and the brain was collected for infarct volume, qPCR and immunoblot detection. The levels of tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), interleukin-1beta (IL-1β) and inducible nitric oxide synthase (iNOS) were detected by qPCR. Glutathione (GSH), superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in serum and cerebral cortex were detected. The clock genes were tested by western blot. Based on these results, 17:00–19:00 was selected to administrate Rus for further mechanism study and Nrf2 blocker group was administrated all-trans-retinoic acid (ATRA) at 14:00 for 3 days. ResultsAdministration of Rus reduced cerebral infarcted volume, ameliorated the behavior score and upregulated the mRNA and protein expression of Per1, Bmal1, Clock, Rev-erbα, transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), quinone oxidoreductase 1 (NQO1). Administration of Rus during 17:00–19:00 had better preventive effect than other three time points. Combined administration of ATRA blunted the preventive effect of Rus. ConclusionThe preventive effect of Rus is affected by the time of administration, which was regulated by Nrf2 pathway. Taken together, we provide solid evidence to suggest that different administration time point affect the effectiveness of Rus in alleviating IRI.

The nature of changes in endocrine and immune factors at the initial stage of the formation of chronic wounds

Aim. The work aimed to study the nature of changes in endocrine signals (insulin and cortisol) and bioactive molecules that control inflammation and activation of process synthesis (interleukin-2 (IL-2), interleukin-8 (IL-8), vascular endothelial growth factor (VEGF), amino-terminal propeptide of type III procollagen (PIIINP)), at the initial stage of the formation of chronic wounds in the experiment.
 Materials and methods. The study was conducted in 2 groups of Wistar rats. The first group is represented by intact animals. Animals of the second group were modeled chronic wounds. On the 3rd day of the experiment, all animals were euthanized. The concentration of studied bioactive molecules was measured in blood serum by enzyme immunoassay. Selected areas of the wound were subjected to histological examination.
 Results. The levels of cortisol, insulin, VEGF, IL-2, and PIIINP in animals with chronic wounds at the stage of inflammation were significantly higher compared to intact animals. A tendency towards a decrease in the expression of IL-8 was shown. Relationships between the levels of bioactive molecules in animals of different groups were studied. According to the results of studies a correlation between the concentrations of IL-8 and cortisol in rats of the intact group was established. In the group of rats on the 3rd day after wound induction, a positive correlation was noted between the levels of IL-8 and IL-2. Wound defects healed under the scab. Microscopic analysis showed that the histoarchitectonics of tissues in the area of damage on the 3rd day corresponded to the classic manifestations of the process of healing wounds at this stage. The main part of the wound was filled with fibrin strands with a predominantly inflammatory cellular component. In some areas, signs of growth and development of young granulation tissue were noted. In the marginal sections, signs of re-epithelialization of the wound defect were observed.
 Conclusions. Even though in our study, the histoarchitectonics of tissues in the area of damage corresponded to the classical manifestations of the wound healing process at the stage of inflammation, the obtained changes and the relationship between endocrine and immune factors may be harbingers of the formation of the chronic wound in the future.

Agmatine improves liver function, balance performance, and neuronal damage in a hepatic encephalopathy induced by bile duct ligation.

In the current study, we investigate whether oral administration of agmatine (AGM) could effectively reduce motor and cognitive deficits induced by bile duct ligation (BDL) in an animal model of hepatic encephalopathy (HE) through neuroprotective mechanisms. The Wistar rats were divided into four groups: sham, BDL, BDL+ 40mg/kg AGM, and BDL+ 80mg/kg AGM. The BDL rats were treated with AGM from 2 weeks after the surgery for 4 consecutive weeks. The open field, rotarod, and wire grip tests were used to assess motor function and muscle strength. The novel object recognition test (NOR) was performed to evaluate learning and memory. Finally, blood samples were collected for the analysis of the liver markers, the animals were sacrificed, and brain tissues were removed; the CA1 regions of the hippocampus and cerebellum were processed to identify apoptosis and neuronal damage rate using caspase-3 immunocytochemistry and Nissl staining. The serological assay results showed that BDL severely impaired the function of the liver. Based on histochemical findings, BDL increased the neuronal damage in CA1 and Purkinje cells, whereas apoptosis was significantly observed only in the cerebellum. AGM treatment prevented the increase of serum liver enzymes, balance deficits, and neuronal damage in the brain areas. Apoptosis partially decreased by AGM, and there were no differences in the performance of animals in different groups in the NOR. The study suggests AGM as a potential treatment candidate for HE because of its neuroprotective properties and/or its direct effects on liver function.

Limited diversity of epibiont bdelloid rotifers and no pattern of codiversification with the highly diverse endemic amphipods of a coastal zone of Lake Baikal

An extreme radiation of hundreds of species of different groups of animals occurred in Lake Baikal, Siberia, Russia; among them, amphipods represent one of the most remarkable groups of invertebrates with about 350 endemic species. Amphipods host associated epibiont rotifers, and the aim of the study is to explore the possibility that bdelloid rotifers living as epibionts on amphipods in Lake Baikal coevolved with their hosts and diversified with species-specific host–epibiont associations. We sampled 148 individual amphipods belonging to 16 species and isolated all epibiont bdelloids from them, discovering that only one bdelloid species, Embata parasitica, lives associated with at least six amphipod species belonging to three different families. Similar to what is known in most other bdelloid species, the morphospecies Embata parasitica from Lake Baikal is likely to be a complex of cryptic species, as suggested by the high genetic diversity we found in one mitochondrial marker sequenced from several animals. Yet none of the divergent genetic lineages seemed to be associated to only one or a few amphipod species. In addition, nine bdelloid species were found living in the lake, increasing the known diversity of the area to 12 bdelloid species.

The Significance and Ethics of Digital Livestock Farming

The emergence of precision and digital livestock farming presents an opportunity for sustainable animal farming practices that enhance animal welfare and health. However, this transformation of modern animal farming through digital technology has several implications for the technological, social, economic, and environmental aspects of farming. It is crucial to analyze the ethical considerations associated with the digitalization of modern animal farming, particularly in the context of human–animal relationships and potential objectification. This analysis can help develop frameworks for improving animal welfare and promoting sustainability in animal farming. One of the primary ethical concerns of digital livestock farming is the potential for a digital divide between farmers who have access to advanced technologies and those who do not. This could lead to a disparity in animal welfare and health outcomes for different groups of animals. Additionally, the use of artificial intelligence in digital livestock farming may lead to a loss of personal connection between farmers and animals, which could impact the animal’s well-being. Another ethical concern of digital livestock farming is the potential for the objectification of animals as mere data points. The use of sensors and other monitoring technologies can provide valuable data on animal health and behavior, but it is important to remember that animals are sentient beings with complex emotional and social needs. The use of digital technologies should not lead to neglect of animal welfare or a lack of human responsibility toward animals. Furthermore, social context becomes essential while integrating technologies in livestock farming to overcome ethics. By considering the cultural and societal norms of different communities, we can ensure that the use of digital technologies does not undermine these values. To address these ethical challenges, the development of standards and codes of conduct for the adoption and implementation of digital livestock farming tools and platforms can help ensure that animal welfare and sustainability are prioritized. This can help alleviate the privacy concerns of stakeholders and improve sustainability in animal farming practices. Additionally, the use of virtual and augmented reality technologies can provide a way to enhance human–animal interactions and provide more personalized care to animals, further promoting animal welfare.

Thermally Dependent Behaviour of Cold-Blooded Animals: Overcoming Two Temperature Barriers on the Way to Warm-Blooded

Background: The temperature preferences of cold-blooded animals are different for different groups of animals and are closely related to their evolutionary status and level of adaptive capabilities. The range of preferred temperatures for reptiles (28°C - 32°C) coincides with the zone of temperature-dependent increase in the rate of the M-cholinergic reaction in the brain, which was previously found in warm-blooded neurons. The growth of the M-cholinergic process contributes to the transition of adaptive behavior to a higher level. Of the cold-blooded, only reptiles actively use thermoregulatory behavior as a tool to achieve a temperature optimum. This paper is devoted to the study of the behavior of red-eared turtles aimed at reaching the range of preferred temperatures. Temperature conditions necessary for the survival of different groups of cold-blooded animals are compared and the reason for temperature preferences is discussed. Methods: The behavior of turtles was studied under conditions of variable solar radiation and in a 3-section terrarium with gradual temperature ranges: 23°C - 24°C; 28°C - 31°C and 45°C - 50°C. Results: It was found that prolonged (up to 1.5 hours) heating at a temperature of 45°C - 50°C was the best way to achieve the preferred temperatures. This method of heating results in increasing the temperature of the shell to an average of 37°C, followed by a very slow decrease during 4 - 6 hours within the limits that closely coincided with the preferred level. Conclusion: The results obtained demonstrate that the main function of the turtle shell is to accumulate heat and keep it for a long time in a variable climate. The preferred temperature achieved in this case is necessary for the temperature-dependent transition of the rate of the M-cholinergic reaction in the brain to a higher level, which creates more significant adaptive capabilities. The temperature range of the M-cholinergic process has two inflection points at 27°C - 29°C and 34°C - 36°C. In accordance with these values, the temperature preferences of cold-blooded animals are divided into three groups: 0°C - 28°C (fish and amphibians); 28°C - 32°C (reptiles) and 34°C - 36°C (dinosaurs). Different ranges of preferred temperatures correspond to three stages of adaptive development.

THE EFFECTIVENESS OF COMBINED USE OF IMMUNO- AND CHEMOTHERAPY WITH DIFFERENT METASTATIC POTENTIAL TUMORS

Summary. Aim: to assess the effectiveness of the combined use of immune and chemotherapy with different metastatic tumors. Object and methods: two experimental models were used: Ehrlich adenocarcinoma (ACE) and Lewis lung carcinoma (CLL). As a means of chemotherapy used cisplatin, biotherapy – B. subtilis IMB B-7724 lectin, which in low concentrations has immunostimulating activity. Animals of different groups were introduced in mono-mode or theіr combination. The standard tumor growth and metastasis rates were evaluated. Upon completion of treatment, the activity of nonspecific immunity effectors was evaluated. The statistical analysis of the results was performed according to the generally accepted methods of variation statistics. Results: on both models of tumor growth, antitumor and antimetastatic efficacy is demonstrated as a means of immunotherapy of B. subtilis IMB B-7724 lectin. The severity of the effect depended on the mode of its introduction (mono-mode, combination with chemotherapy) and model tumors: for mice with ACE more effective was the introduction of lectin in mono-mode (the tumor growth index, TGI=53,2%); with LLC – combined injection of lectin and cisplatin (TGI =46,5%, metastasization inhibition index, МІІ=78,6%). The effect in both cases is associated with the ability of the lectin to remodel and maintain for a long time on the background of tumor growth polarization of macrophages in the direction of the proinflammatory state of M1 (increase in production of NO against the background of reduction of arginase activity (ARG)). This is evidenced by a strong positive correlation between NO/ARG and TGI level (r = 0.90, p <0.05 (ACE); also between NO/ARG and IIM level (r = 0.98, p <0.05 (CLL)). Conclusions: antitumor and antimetastatic efficiency of various regimens of use of chemo-/immunotherapy is due to the activation of nonspecific immune response and depends on both the immunogenic characteristics of tumors and on the initial physiological features of the immune system.