Culicoides, among the tiniest and most abundant hematophagous insects globally, serve as vectors for a variety of pathogens such as viruses, bacteria, parasites, protozoa, and nematodes. This study aimed to identify Culicoides species and assess their spatial distribution and seasonal occurrence in selected districts of the Central, South, and West Gondar zones, Northwest Ethiopia. A cross-sectional study was conducted between January to July 2023. A total of 44 UV light- onderstepoort traps were deployed in the study districts near specific areas. The traps were operational from dusk (6:00 PM) until dawn (6:00 AM) and were suspended at a height of 1.5 to 2 m above the ground. Poisson regression was used to assess associations, the Shannon diversity index to measure diversity, and QGIS 3.22.6 to create maps. In this study, 8,857 Culicoides were captured across the 44 trapping sites. Of the total flies captured flies, 8,838 were identified as belonging to 12 distinct species, while the classification of the remaining 19 flies remained unclear. Notably, C. kingi (54.01%) was the most prevalent species, followed by C. imicola (44.55%). The abundance of Culicoides observed from January to late April (3505) was significantly lower compared to the wet season (5355), with a marked increase in the capture of C. kingi (2499) from May to late July. A statistically significant association (p < 0.05) was observed between the occurrence of Culicoides and factors such as district, sampling point, and season. Spatial analysis revealed that C. kingi had a broader range of suitability than other Culicoides species, with high suitability observed in East Dembia. The diversity index analysis indicated that Culicoides species diversity was higher in samples from animal pens (H = 0.73) and during the wet season (H = 0.75). Additionally, this study documented the presence of eight Culicoides species namely C. corsicus, C. kibunensis, C. reioxi, C. kiouxi, C. saharienines, C. desertorum, C. reithi, and C. festivipennis, which have not been previously documented in Ethiopia. In conclusion, the study highlighted that the occurrence of Culicoides species was higher in East Dembia, with moderate presence in Wegera and West Armacho. Further research is needed to assess the impact of various Culicoides species on animal and human health, as well as their economic implications, and to develop corresponding control strategies based on these findings.
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