Animal-derived Products Research Articles

Feeding Strategies and Quality Assessments of Animal-Derived Products

Feeding strategies play an important role in animal production systems by directly influencing animal health, productivity, and the quality of animal-derived products (meat, eggs, and milk) [...]

Integrated analysis of muscle lncRNA and mRNA of Chinese indigenous breed Ningxiang pig in four developmental stages.

Meat and its derivatives serve as crucial sources of protein, vitamins, minerals, and other essential nutrients for humans. Pork stands as China's primary animal-derived food product consumed widely across diverse dietary structures; evaluating intramuscular fat content becomes pivotal in assessing its quality standards. Nonetheless, the intricate molecular mechanisms governing intramuscular fat deposition remain elusive. Our study utilized sequencing technology to scrutinize longitudinal development stages within Ningxiang pig's longest dorsal muscles aiming to unravel these underlying mechanisms. In three distinct comparisons (30d vs. 90d, 90d vs. 150d and 150d vs. 210d) there were 578, 1,000 and 3,238 differentially expressed mRNA, along with 16, 158 and 85 lncRNAs were identified. STEM analysis unveiled six enriched model profiles for lncRNAs while seven such profiles emerged for mRNAs; notably, multiple shared model profiles existed between both RNA types. Enriched analysis highlighted numerous genes from mRNA profile8 and lncRNA profile7 significantly associated with pathways linked to fat deposition. Weight Gene Co-Expression Network Analysis (WGCNA) revealed that differential expression modules (DMEs) & differential expression lncRNAs primarily clustered within cyan, dark slate blue and pale turquoise modules. Furthermore, target genes PKD2 (MSTRG21592.MTRSG8859 and MTRSG18175), COL5A1 (MTRSG9969 and MTRSG180) and SOX13 (MTRSG21592 and MTRSG9088) as core components all intricately tied into processes related to fat deposition. This study lays the groundwork for deeper exploration into the molecular mechanisms underlying LDM fat deposition traits, and it also presents candidate genes for future molecular marker-assisted breeding.

Assessment of residues from common pesticides and associated risks in Pakistan.

Pesticide residues have become an escalating concern in Pakistan, raising significant risks to both public health and the environment. This review overviews the current status of pesticide contamination in the country, with a specific focus on residue levels in relation to Maximum Residue Limits (MRLs) and a comparative analysis with regional and global counterparts. Several studies reveal that 30% to 70% of tested food products, including fruits, vegetables, and animal-derived products, especially in Punjab and Sindh, exceed established MRLs. This level of contamination is considerably higher than in neighboring countries such as India, where MRL exceedance ranges from 20 to 30%, and Bangladesh, which reports lower levels due to stricter regulatory efforts. In comparison, developed countries, including those in the European Union and the United States, report less than 5% of food samples exceeding MRLs, often falling below 1% due to stringent safety standards. The high prevalence of pesticide residues in Pakistan poses severe health risks, including neurological disorders, reproductive problems, and cancer, particularly in vulnerable groups like children and pregnant women. Key factors contributing to these elevated pesticide levels include insufficient knowledge of safe pesticide use, the unregulated application of banned or expired products, improper safety protocols, and malfunctioning equipment. A prevailing reliance on pesticides as the sole pest control method further hinders the adoption of Integrated Pest Management (IPM) strategies. In conclusion, pesticide residue levels in Pakistan are above permissible MRLs compared to regional and global standards, underscoring the need for urgent improvements in pesticide regulation, enforcement, and the promotion of sustainable pest control methods like IPM to better protect human health and the environment.

Moving on from clinical animal-derived surfactants to peptide-based synthetic pulmonary surfactant.

Research on lung surfactant has exerted a great impact on newborn respiratory care and significantly improved survival and outcome of preterm infants with respiratory distress syndrome (RDS) due to surfactant deficiency because of lung immaturity. Current clinical, animal-derived, surfactants are among the most widely tested compounds in neonatology However, limited availability, high production costs, and ethical concerns about using animal-derived products constitute important limitations in their universal application. Synthetic lung surfactant offers a promising alternative to animal-derived surfactant by providing improved consistency, quality and purity, availability and scalability, ease of production and lower costs, acceptance, and safety for the treatment of neonatal RDS and other lung conditions. Third-generation synthetic surfactants built around surfactant protein B (SP-B) and C (SP-C) peptide mimics stand at the forefront of innovation in neonatal pulmonary medicine, while nasal continuous positive airway pressure (nCPAP) has become the standard non-invasive respiratory support for preterm infants. nCPAP can prevent the risk of chronic lung disease (bronchopulmonary dysplasia) and reduce lung injury by avoiding intubation and mechanical ventilation, is a relatively simple technique and can be initiated safely and effectively in the delivery room. Combining nCPAP with noninvasive, preferably aerosol, delivery of synthetic lung surfactant promises to improve respiratory outcomes for preterm infants, especially in low-and-middle income countries.

Use of rice flour to produce plant-based yogurt alternatives.

Plant-based yogurt alternatives (YAs) are in demand due to the societal prevalence of milk sensitivities and allergies and some consumers abstaining from animal-derived products. Producing rice flour YAs has considerable potential because rice flour is hypoallergenic, more economical compared to plant milks, and there are no commercial rice-based YAs. A new higher protein variety of rice was developed, Frontière, which is sold as both brown and white rice. Therefore, the overall goals of this study were (1) to compare physicochemical properties of YAs from Frontière brown (Frontière brown rice flour [FBRF]) and white (Frontière white rice flour [FWRF]) high-protein rice flours to regular protein level rice flours and (2) to evaluate the sensory quality of Frontière YAs compared to commercial plant-based oat yogurt. Rice flours were fermented with Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, and Lacticaseibacillus rhamnosus (a probiotic) to produce FBRF- and FWRF-YAs. A consumer study was conducted to compare FBRF- and FWRF-YAs to a commercially available oat-based YA. Consumers rated the YAs using 9-point hedonic and just-about-right (JAR) scales. Protein, fat, and ash levels were greater, whereas starch levels and peak viscosities were lower for BRFs than for WRFs. The use of BRF resulted in longer fermentation times but higher bacteria counts for YAs. FWRF-YA was preferred in terms of overall flavor and liking, sweetness, and tartness. Purchase intent (PI) for FWRF-YA increased 2.6 times after providing a health claim. This research showed that rice flour can be used to produce YAs with probiotic counts above the minimum recommended, which provides added health benefits for consumers. PRACTICAL APPLICATION: This research provides a possible new use of Frontière high-protein rice flour to produce plant-based yogurts. This will help the rice industry by adding value, and those who are vegan, allergic to casein, or lactose-intolerant will have another option for a plant-based yogurt. Moreover, the greater levels of probiotic bacteria found in the brown rice flour YAs can potentially provide greater health benefits, making brown rice flour a better choice for making rice-based YAs.

Development of a nanobody-horseradish peroxidase fusion-based competitive ELISA to rapidly and sensitively detect Enrofloxacin residues in animal-derived foods

The ability to reliably detect enrofloxacin in animal-derived food products has important health implications. In the present study, a nanobody-horseradish peroxidase fusion specific for ENR was generated to enable a sensitive and rapid competitive ELISA suitable for detecting enrofloxacin in samples of milk and animal tissue. An enrofloxacin hapten generated via the glutaraldehyde method was initially used to immunize an adult Bactrian camel as a means of constructing a phage library. Enrofloxacin−specific nanobodies were then selected through three rounds of biopanning, and HRP-fused versions of these nanobodies were then expressed. Lastly, these nanobodies were used to develop a sensitive cELISA for enrofloxacin detection in milk and animal tissues, with the resultant assay exhibiting an IC50 of 37.41 ng/mL and a linear detection range (IC20-IC80) of 10.89 to 244.34 ng/mL. The limit of detection for this cELISA was 6.48 ng/mL, with 4.66 % cross-reactivity with ciprofloxacin, and recovery rates that ranged from84.99 % to 107.72 % together with an RSD below 10.70 %.

Research Status and Safety Considerations of Animal-Derived Mesh Products

With the development of the economy and technological progress, more and more animal-derived mesh products are being utilized in the medical field for tissue and organ repair and replacement. Owing to the complexity of their structure and production process, these animal-derived meshes still face several challenges in practical applications, such as insufficient mechanical strength, rapid degradation rates, and the detection of harmful leachable substances. Among these challenges, the production process is a key factor affecting product quality. This paper reviews the key aspects of the production process and quality control for animal-derived meshes in China, offering new insights for the quality control and regulatory oversight of such products.

A Lincomycin-Specific Antibody Was Developed Using Hapten Prediction, and an Immunoassay Was Established to Detect Lincomycin in Pork and Milk.

Prolonged consumption of animal-derived foods containing high levels of lincomycin (LIN) residues can adversely impact human health. Therefore, it is essential to develop specific antibodies and immunoassay methods for LIN. This study utilized computational chemistry to predict the efficacy of LIN haptens prior to chemical synthesis, with subsequent confirmation obtained through an immunization experiment. A hybridoma cell line named LIN/1B11 was established, which is specific to LIN. The optimized indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method exhibited high specificity for detecting LIN residues, with an IC50 value of 0.57 ± 0.03 µg/kg. The method effectively detected LIN residues in pork and milk samples, achieving a limit of detection (LOD) ranging from 0.81 to 1.20 µg/kg and a limit of quantification (LOQ) ranging from 2.09 to 2.29 µg/kg, with recovery rates between 81.9% and 108.8%. This study offers a valuable tool for identifying LIN residues in animal-derived food products. Furthermore, the efficient hapten prediction method presented herein improves antibody preparation efficiency and provides a simple method for researchers in screening haptens.

Eficiência do produto homeopático BioBoi® combinado ao manejo adequado da ordenha, no tratamento de mastite em vacas em lactação

The dairy production chain is one of the main economic activities in Brazil, with primary production present in almost all Brazilian municipalities, involving more than one million rural producers, and generating jobs in all segments of the chain. The growing consumption of dairy products, and the pursuit of quality, lead to increasing concern with food and nutritional safety. Among the factors that affect milk composition are management and the incidence of diseases, with mastitis being one of the most frequent infections affecting dairy cattle. This results in economic losses due to reduced production and milk quality, increased costs for labor, medication, and veterinary services, and the early disposal of animals. Treatment with commercial homeopathic products presents an alternative to avoid problems found in the continuous use of allopathic medicines, such as residues in animal-derived products, selection of multi-resistant strains, and reactions in hypersensitive individuals. The general objective of this study was to evaluate the efficiency and effectiveness of using a commercial homeopathic product in the treatment of bovine mastitis when combined with proper milking management. Our specific objectives were to evaluate the milker’s handling before, during, and after milking, to determine the incidence and prevalence of clinical and subclinical cases of mastitis in the herd before and after treatment, and to perform comparative analyses of the data obtained at the beginning and end of the experiment. The results showed that positive behaviors by the milker had greater influence on the cows' behavior, which remained calm and stress-free throughout the milking process, as well as a correlation with obtaining low somatic cell counts (SCC). Regarding the prevalence of mastitis in the herd, although it decreased, there was no statistically significant difference. However, there was evidence of the positive interference of the homeopathic medicine on its incidence. It is concluded that the homeopathic medicine is effective in inhibiting the emergence of new cases of mastitis, as well as in treating acute cases of mastitis and reducing the herd's SCC, being efficient in the control and prevention of mastitis; it is also concluded that the milker plays an important role in this process, making it essential for them to be aware and valued for this.

Purity and pollution: faith vegans’ categorizations of plant-based foods as pure and animal-derived foods as polluting

ABSTRACT Veganism has increased exponentially in the UK in recent years and whilst studies have attended to the redefinition of edibility and the adoption of new relationships with foodstuffs, one area that is noticeably absent from academic scholarship is the intersection with religion. Considering the influence that religion has on understandings of edibility, permissibility, and consumption, research gaps emerge pertaining to how religion and veganism come together to reshape the perceptions and categorizations of food types by vegans of faith. This article draws on a sociological study which recruited 12 Muslim vegans, 12 Jewish vegans, and 12 Christian vegans in the UK and conducted semi-structured qualitative interviews, diary methods, and virtual participant observation to gain a holistic understanding of the faith vegan experience. This article’s main finding is a dichotomous categorization of food types by faith vegans, with plant-based foods being seen as pure and animal-derived products being seen as polluting and inedible. Further, these understandings are intertwined with religious ethics, principles, and teachings in order to construct veganism as both God’s ideal diet and correct religious observance, whilst animal consumption is constructed as human greed, weakness, and a violation of what is perceived to be the ethical spirit of religion.

A novel HCR-CRISPR/Cas12a immunosensor for the sensitive detection of pesticide residues in animal-derived foods

Pesticides are widely used in the cultivation and breeding of agricultural products. However, pesticide residue monitoring has focused mainly on fruits and vegetables and has not focused on animal-derived foods. Therefore, a novel approach for determining pesticide residues in animal-derived food products via an immunosensor based on a chain hybridization reaction (HCR) with CRISPR/Cas12a is presented. Triazophos pesticide was used as a research object. This method establishes a competitive immunoreaction system in which OVA-hapten is encapsulated on the surface of the enzyme label plate, competing with pesticide molecules bound to antibodies on the surface of the nanogold composite probe. Integrating the immunoreaction with two signal amplification mechanisms, HCR and CRISPR/Cas12a, ensures heightened method sensitivity while maintaining enhanced convenience and speed. The method's detection range spans from 0.01 to 50 ng/mL, with a remarkably low detection limit of 0.013 ng/mL. Moreover, in practical sample applications, the assay exhibited a robust correlation with GC‒MS. This approach uniquely offers real-time, cost-effective, and onsite data, providing a more comprehensive and timely foundation for establishing standards on pesticide residues in animal-derived foods, bolstering food safety surveillance, and upholding public health. Moreover, the proposed HCR-CRISPR/Cas12a immunosensor effectively improved the sensitivity and accuracy of pesticides detection, offering a promising and versatile platform for food safety detection.

Eco-friendly electrochemical assay of oxytetracycline and flunixin in their veterinary injections and spiked milk samples

Two solid-contact electrochemical sensors were developed for detection of each of oxytetracycline HCl (OXY), and the co-formulated non-steroidal anti-inflammatory drug flunixin meglumine (FLU) in veterinary formulations and animal-derived food products. The designed sensors were based on a glassy carbon electrode as the substrate material and high molecular weight polyvinyl chloride (PVC) polymeric ion-sensing membranes doped with multiwalled carbon nanotubes (MWCNTs) to improve the potential stability and minimize signal drift. For determination of OXY, the sensing membrane was modified with potassium tetrakis (4-chlorophenyl) borate (K-TCPB), which was employed as a cation exchanger, and 2-hydroxypropyl-β-cyclodextrin (HP-ßCD), which was used as an ionophore. A linear response within a concentration range of 1 × 10− 6-1 × 10− 2 M with a slope of 59.47 mV/decade over a pH range of 1–5 was recorded. For the first time, two potentiometric electrodes were developed for determination of FLU, where the sensing membrane was modified with tetra dodecyl ammonium chloride (TDDAC) as an anion exchanger. A linear response within a concentration range of 1 × 10− 5-1 × 10− 2 M and a slope of -58.21 mV/decade over a pH range of 6–11 was observed. The suggested sensors were utilized for the selective determination of each drug in pure powder form, in veterinary formulations, and in spiked milk samples, with mean recoveries ranging from 98.50 to 102.10, and without any observed interference. The results acquired by the proposed sensors were statistically analyzed and compared with those acquired by the official methods, and the results showed no significant difference.Graphical

UTILIZATION OF NANOTECHNOLOGY IN VETERINARY MEDICINE

In veterinary medicine, particularly in diagnostics, drug administration, and reproductive biology, nanotechnology has made significant contributions. By enabling the precise delivery of unstable or insoluble substances, reducing systemic toxicity, and prolonging the action of the active ingredient at the desired site, nanoparticles enhance the effectiveness of treatments. This translates to fewer doses, reduced costs, and less waste from animal-derived products. Furthermore, nanotechnology aids in diagnostics by detecting pathogens and mapping molecular profiles related to diseases. The sensitivity of tests, increased by nanoparticles, allows for more effective detection of contaminants and microbial agents in animal-derived products, which is crucial for food safety. Nanoparticles and nanotubes are also used in reproductive biology to improve fertility, extend sperm longevity, and facilitate semen purification for artificial insemination. Additionally, implanted nanotubes can monitor hormone levels, aiding in the identification of estrus and the selection of breeders. Despite these advancements, it is crucial to monitor the risks and environmental impacts of nanotechnological technologies to ensure their safety and effectiveness in veterinary medicine. The objective of this work is to explore and evaluate current applications of nanotechnology in veterinary medicine, highlighting its benefits, challenges, and potential for future advancements.

VITAMIN B2 AND ITS STATUS IN VEGETARIANS AND VEGANS

The main goal of this review was to briefly highlight the key points of today’s knowledge about the role and metabolism of vitamin B2 as well as to compare its status in vegans, vegetarians, and omnivores according to present data. Biologically active forms of water-soluble vitamin B2 (riboflavin) are represented by flavin mononucleotide (FMN) and flavin adenine diphosphate (FAD), which are coenzymes in the reactions of the electron transport chain and the pyruvate dehydrogenase complex. Riboflavin is also involved in the metabolism of fatty acids, the synthesis of cholesterol and steroid hormones. Riboflavin also participates in the folate cycle. Vitamin B2 is crucial for the metabolism of other vitamins. FMN is necessary for the for-mation of the active form of vitamin B6, while FAD is necessary for the synthesis of niacin. Severe clinical insufficiency of vitamin B2 is not common. Besides insufficient riboflavin consumption, hypothyroidism can also be a cause of vitamin B2 deficiency as thyroxine regulates the conversion of ribo-flavin to its active forms  FMN and FAD. Lack of riboflavin can cause tissue damage; especially of the epithelial, reproductive, and nervous system. The variety of its manifestations is related to the fact that riboflavin deficiency also disrupts the metabolism of vitamins B6, or PP. Similarly, riboflavin deficiency can also disrupt the folate cycle, increasing the level of homocysteine which damages vascular intima and leads to endothelial dysfunction and atherosclerosis as a result. Rapid urinary excretion of riboflavin makes it safe even if taken in a high dose, changing the urine colour into bright-yellow. This safety of riboflavin makes it unharmful to be used as an oral tracer for monitoring compliance in clinical research. Ultra-high doses of ribo-flavin (more than 400 mg/day) can cause diarrhoea or polyuria. The content of vitamin B2 in animal-derived products is higher than in plant-based ones. This also determines its lower intake in vegans and vegetarians compared to omnivores. While there is a shred of evidence that plant-based diets in-crease the production of bioavailable riboflavin by the gut microbiota, this does not appear to be sufficient to completely compensate for the vegetari-ans’/vegans’ reduced dietary intake of vitamin B2, as most of the researches point the fact that vegans and vegetarians are more prone to lack vita-min B2 than omnivores. At the same time, it should be noted that there exists no universally adopted optimal method for assessing the status of vit-amin B2 in the body. Further research and standardization of methods may ease to assess the prevalence and risks of riboflavin deficiency in various dietary groups

Electrochemical Aptasensor with Antifouling Properties for Label-Free Detection of Oxytetracycline.

Oxytetracycline (OTC) is a widely employed antibiotic in veterinary treatment and in the prevention of infections, potentially leaving residues in animal-derived food products, such as milk, that are consumed by humans. Given the detrimental effects of prolonged human exposure to antibiotics, it has become imperative to develop precise and sensitive methods for monitoring the presence of OTC in food. Herein, we describe the development and results of a preliminary label-free electrochemical aptasensor with antifouling properties designed to detect OTC in milk samples. The sensor was realized by modifying a gold screen-printed electrode with α-lipoic acid-NHS and an amine-terminated aptamer. Different electrochemical techniques were used to study the steps of the fabrication process and to quantify OTC in the presence of the Fe(CN)64-/Fe(CN)63- redox couple The detectable range of concentrations satisfy the maximum residue limits set by the European Union, with an limit of detection (LOD) of 14 ng/mL in phosphate buffer (BP) and 10 ng/mL in the milk matrix, and a dynamic range of up to 500 ng/mL This study is a steppingstone towards the implementation of a sensitive monitoring method for OTC in dairy products.

A sensitive tobramycin electrochemical aptasensor based on multiple signal amplification cascades

The residue of tobramycin, a broad spectrum antibiotic commonly used in animal husbandry, has evitable impact on human health, which may cause kidney damage, respiratory paralysis, neuromuscular blockade and cross-allergy in humans. Sensitive monitoring of tobramycin in animal-derived food products is therefore of great importance. Herein, a new aptamer electrochemical biosensor for sensing tobramycin with high sensitivity is demonstrated via exonuclease III (Exo III) and metal ion-dependent DNAzyme recycling and hybridization chain reaction (HCR) signal amplification cascades. Tobramycin analyte binds aptamer-containing hairpin probe to switch its conformation to expose the toehold sequence, which triggers Exo III-based catalytic digestion of the secondary hairpin to release many DNAzyme strands. The substrate hairpins immobilized on the Au electrode (AuE) are then cyclically cleaved by the DNAzymes to form ssDNAs, which further initiate HCR formation of lots of long methylene blue (MB)-tagged dsDNA polymers on the AuE. Subsequently electro-oxidation of these MB labels thus exhibit highly enhanced currents for sensing tobramycin within the 5–1000 nM concentration range with an impressive detection limit of 3.51 nM. Furthermore, this strategy has high selectivity for detecting tobramycin in milk and shows promising potential for detect other antibiotics for food safety monitoring.

Simultaneous determination of phenothiazine drugs and their metabolites residues in animal derived foods by high performance liquid chromatography tandem mass spectrometry

Due to their potential entry into the human body via the food chain, phenothiazine drugs and their metabolites pose specific health hazards, making their detection in edible tissues of paramount importance. In this research endeavor, a highly sensitive multi-residue analytical method has been devised for the detection of phenothiazine drugs and their metabolites in animal-derived food products, utilizing high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The preparation of samples involved the use of 1% formic acid in acetonitrile as the extraction solvent following sample weighing, with multiple extraction steps diligently performed to ensure optimal outcomes. The sample, post-extraction, was directly introduced into the purification column, yielding significant time savings and rendering the process more environmentally sustainable. This procedural step effectively eliminated interfering substances from the sample matrix, including proteins, salts, and phospholipids. Analysis of the extracted samples was conducted through HPLC-MS/MS, employing an electrospray ionization (ESI) ion source. A comprehensive panel of 20 phenothiazine drugs and their metabolites was successfully resolved within a mere 10-min chromatographic run. A robust linear correlation (R2 ≥ 0.99) was attained within a judiciously chosen range of concentrations. Method validation was meticulously conducted across multiple animal species, including trout, white shrimp, pork, beef and chicken. The developed analytical method has been effectively deployed for the screening and quantification of phenothiazine drugs in real-world samples sourced from commercial livestock and aquatic products.

Vitamin B12 (cobalamin) deficiency in gastroenterological diseases - review

Introduction: Vitamin B12 is a water-soluble vitamin found primarily in animal-derived products such as red meat, dairy, and eggs. An essential substance for its absorption is the intrinsic factor, produced by the parietal cells of the stomach. After absorption in the distal ileum, cobalamin acts as a coenzyme in DNA synthesis and the metabolism of fatty acids and homocysteine. In addition to its well-known influence on the nervous system, it has been associated with gastrointestinal diseases according to numerous authors. Objective: The objective of this study is to gather and analyze literature regarding the associations between vitamin B12 deficiency and gastrointestinal diseases, including gastroesophageal reflux disease (GERD), gastritis, inflammatory bowel disease (IBD), small intestinal bacterial overgrowth (SIBO), and hepatitis C (HCV). Materials and Methods: A literature review was conducted using the PubMed database with the following search terms: vitamin B12, cobalamin, deficiency, gastrointestinal diseases, GERD, IBD, SIBO, HCV. Current Knowledge: Vitamin B12 deficiency can be caused by autoimmune factors, dietary insufficiencies, toxin exposure, and malabsorption disorders related to gastrointestinal diseases. Gastrointestinal conditions such as GERD and IBD can both lead to and be exacerbated by cobalamin deficiency. Furthermore, vitamin B12 deficiency may be worsened by prolonged use of proton pump inhibitors (PPIs) and Helicobacter pylori infection. Conclusions: An adequate amount of vitamin B12 is crucial for gastrointestinal health. Deficiency can significantly impact the course and symptoms of many gastrointestinal diseases. Therefore, monitoring and supplementation of vitamin B12 should be an integral part of managing patients with gastrointestinal conditions.

Responsible innovation: Mitigating the food safety aspects of cultured meat production.

There is much interest in cultured (cultivated) meat as a potential solution to concerns over the ecological and environmental footprint of food production, especially from animal-derived food products. The aim of this critical review is to undertake a structured analysis of existing literature to (i) identify the range of materials that could be used within the cultured meat process; (ii) explore the potential biological and chemical food safety issues that arise; (iii) identify the known and also novel aspects of the food safety hazard portfolio that will inform hazard analysis and risk assessment approaches, and (iv) position a responsible innovation framework that can be utilized to mitigate food safety concerns with specific emphasis on cultured meat. Although a number of potential food safety hazards are identified that need to be considered within a food safety plan, further research is required to validate and verify that these food safety hazards have been suitably controlled and, where possible, eliminated. The responsible innovation framework developed herein, which extends beyond hazard analysis and traditional risk assessment approaches, can be applied in multiple contexts, including this use case of cultured meat production.

Development and Characterization of Hybrid Meat Analogs from Whey Protein-Mushroom Composite Hydrogels.

There is a need to reduce the proportion of animal-derived food products in the human diet for sustainability and environmental reasons. However, it is also important that a transition away from animal-derived foods does not lead to any adverse nutritional effects. In this study, the potential of blending whey protein isolate (WPI) with either shiitake mushroom (SM) or oyster mushroom (OM) to create hybrid foods with enhanced nutritional and physicochemical properties was investigated. The impact of OM or SM addition on the formation, microstructure, and physicochemical attributes of heat-set whey protein gels was therefore examined. The mushroom powders were used because they have relatively high levels of vitamins, minerals, phytochemicals, and dietary fibers, which may provide nutritional benefits, whereas the WPI was used to provide protein and good thermal gelation properties. A variety of analytical methods were used to characterize the structural and physicochemical properties of the WPI-mushroom hybrids, including confocal microscopy, particle electrophoresis, light scattering, proximate analysis, differential scanning calorimetry, thermogravimetric analysis, dynamic shear rheology, textural profile analysis, and colorimetry. The charge on whey proteins and mushroom particles went from positive to negative when the pH was raised from 3 to 9, but whey protein had a higher isoelectric point and charge magnitude. OM slightly increased the thermal stability of WPI, but SM had little effect. Both mushroom types decreased the lightness and increased the brownness of the whey protein gels. The addition of the mushroom powders also decreased the hardness and Young's modulus of the whey protein gels, which may be because the mushroom particles acted as soft fillers. This study provides valuable insights into the formation of hybrid whey protein-mushroom products that have desirable physiochemical and nutritional attributes.