This study aims to address the problem of improving animal image classification accuracy using different versions of MobileNet. Accurate animal classification plays a vital role in biodiversity protection, environmental monitoring, and agriculture. The research is significant because existing studies focus on specific models and datasets, leaving a gap in the comparative performance analysis of MobileNet versions. To address this issue, MobileNet V1, V2, and V3 models were utilized, both with and without ImageNet pre-trained weights. The models were trained on a dataset composed of 30,179 images from two sources, covering 13 animal categories. The experiment involved training the models over 10 epochs using a standard configuration of the TensorFlow framework, with accuracy serving as the primary evaluation metric.The results showed that MobileNet V3Large, with pre-trained weights, achieved the highest accuracy (97.43%), outperforming V1 and V2. Using pre-trained weights consistently enhanced performance, as models without pre-training exhibited lower accuracy and slower convergence. This study contributes by providing a comprehensive comparison of MobileNet versions in animal classification tasks, demonstrating the importance of pre-training and model architecture optimization for achieving high accuracy in image classification.