Animal Blood Groups Research Articles

Генотипический состав стада черно-пестрой породы и его фенотипическая характеристика в связи с голштинизацией

Abstract. The article presents the results of using the genetic potential of the Holstein breed to improve black-and-white cattle. The purpose of the research was to analyze the influence of Holstein on the genetic structure of the herd and its milk productivity for the subsequent use of the results of the analysis in breeding and breeding work with animals. The objects of research were pure-bred animals of black-motley breed and crossbreeds of different genotypes from their crosses with bulls-producers of Holstein breed. Methods. The work used data from zootechnical and pedigree farm accounting, appraisal of livestock. Reliability of the origin of animals and the genetic structure of the herd was established by immunogenetic examination of the blood group systems (antigenic composition and alleles of the B-system). Results. It was established that under the same conditions of feeding and keeping, the milk productivity of the crossbred cows for the first lactation, depending on the blood content in the Holstein breed, was 97–550 kg more milk than pure-bred peers of black-motley breed. The most desirable for further breeding work are the genotypes of 5/8 and 3/4 blood animals. The use of Holstein bulls in this herd led to a significant change in the concentration of individual antigens and alleles of the B-system of blood groups in animals in comparison with the population of black-motley cattle. Scientific novelty. For the first time in the conditions of the Saratov region, studies were conducted to assess the population of black-motley cows and their Holstein crossbreeds by a set of economically useful traits and the efficiency of their breeding.

The effect of silicon compounds on hematological indicators and content of lipids in blood of rabbits

The aim of the study was to find out the effect of casting of citrate nanosіlicon absorption and metasilicate sodium on the hematological parameters and lipid content and their fractional composition in blood plasma preparatory period 10 days prior to insemination and 20 days of lactation. The research was carried out on 60 rabbits of the Hyla hybrid, divided into three groups (control and two experimental), with 20 animals in each. The control animals were fed without limitation full-grain granulated feed with free access to water. Animals of the first experimental group (E-I) fed the diet of the control group and during the course of the day poured out the silicon citrate obtained using the nanotechnology method, 50 mkg Si/kg body weight with water. Samples of the second experimental group (E-II) feed the diet of the control group and set the sodium metasilicate (Na2SiO3H2O) 2.5 mg Si/kg body weight with water. The trial lasted 95 days, including the preparatory period of 10 days, the trial – 85 days. In the preparatory period of 10 days from the beginning of the study and in a trial of 20 days of lactation (65 days of supplements) in the rabbits, samples of blood from the marginal ear vein were taken. Hematologic studies were performed using the automatic hematologic analyzer “Orphee Mythic 18”, in the blood plasma, the content of total lipids and their fractional composition were determined. It was established that the number of erythrocytes and hemoglobin concentration in the blood of the rabbits of experimental group, which were given silicon citrate, were respectively higher by 15.2% (P < 0.05) and 12.0% (P < 0.01) by 20 days lactation versus control. This may indicate a more pronounced effect of the organic compound of silicon on the hematopoietic function of the body of the rabbits. According to white blood, the differences from control were found during the presentation of supplements with higher (P < 0.05) changes in 20 days of lactation in animal blood and experimental group. It was noted that the content of triacylglycerols in plasma of blood of groups I and II was lower by 31.2 and 32.8%, respectively (P < 0.05) compared to control. In blood plasma of animals in experimental groups an increase in the fractions of phosphatidylcholine, sphingomyelin and lysolecetin was observed compared to control. Such a redistribution was due to the reduction of the fractions of phosphatidic acid, phosphatidyl ethanolamine and phosphatidylinositol, and apparently, is associated with changes in the activity of the enzymes concerned. Hematological studies and determination of the fractional composition of lipids and phospholipids of blood plasma of the rabbits show positive changes that contribute to the metabolic accumulation of energy and plastic components of their organism.

Important genealogical bloodlines of Pinzgau cattle in Slovakia

Received: 2016-05-26 | Accepted: 2016-06-13 | Available online: 2017-03-31 http://dx.doi.org/10.15414/afz.2017.20.01.13-15 The aim of this paper was to analyze sire bloodlines within the Slovak Pinzgau cattle population. There were 13 genealogical bloodlines found together. The most widespread bloodline was Apollo. The average estimated breeding values according to sire bloodlines were predominately negative. The best milk production EBV were found in Benito line (-39.35 kg). The best growth ability according to EBV for weaning weight (210 days) was found in Karst bloodline (+3.39 kg). Significant influence of genealogical bloodline was found on EBV for weight in 365 days, protein production and weaning weight (210 days). References ANTALIK, P. and STRAPAK, P. (2010). The evaluation of milkability of Slovak Pinzgau cattle by lactocorder. Slovak J. Anim. Sci., vol. 43, no. 4, pp. 173–178. ERHARD L. and. SCHMID, D. O. (1965). Blood Group Studies on Pinzgau-Cattle. Matousek, J. (ed). Blood Groups of Animals. Proceedings of the 9th European Animal Blood Group Conference (First Conference Arranged by E.S.A.B.R.) held in Prague, August 18–22, 1964. doi:http://dx.doi.org/10.1007/978-94-017-6289-2_5 KADLECIK, O. et al. (2004) Development of dual-purpose Pinzgau cattle. Nitra: Slovak University of Agriculture in Nitra. 128 p. KASARDA, R. and KADLECIK, O. (2007) An economic impact of inbreeding in the purebred population of Pinzgau cattle in Slovakia on milk production traits. Czech Journal of Animal Science, vol. 15, no. 1, pp. 8–13. KASARDA, R. et al. (2014) Influence of mating systems and selection intensity on the extent of inbreeding and genetic gain in the Slovak Pinzgau cattle. Czech Journal of Animal Science, vol. 59, no. 5, pp. 219–226. PAVLIK, I. et al. (2014) Joint genealogical analysis as a tool for diversity evaluation in Pinzgau cattle populations. Archiv Tierzucht, vol. 57, no. 14, pp. 1–12. SAS. (2009) User´s Guide Version 9.2. SAS/STAT ® SAS Institute Inc. Cary, NC, USA. SIDLOVA, V. et al. (2014a) Microsatellite analysis of population structure in Slovak Pinzgau cattle. Acta Agraria Kaposvarienis, vol. 18, supplement 1, pp. 24–29. SIDLOVA, V. – KASARDA, R. – MORAVCIKOVA, N. (2014b). Genealogic structure of Slovak Pinzgau cattle population. MendelNet 2014: International Scientific Conference Proceedings, Brno, November 19–20, 2014. pp. 187–191.

Identification and localization of an adhesin on the surface of Tritrichomonas foetus.

Tritrichomonas foetus is a mucosal parasite of the urogenital-vaginal tract of cattle that strongly adheres to erythrocytes, which suggests that it presents an adhesin that recognizes red blood cells from different animal species and blood groups. In the present report we describe a cell-fractionation method for obtainment of a membrane fraction of T. foetus, which adhered to red blood cells. The T. foetus adhesin was obtained after parasite lysis and fractionation followed by ultracentrifugation, whereby a 100,000-g pellet fraction showed a strong hemagglutinating activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of this fraction, of erythrocyte ghosts, and of ghosts allowed to interact with the parasite membrane fraction revealed the presence of a 100-kDa protein as the putative adhesin. Polyclonal antibodies obtained in rabbits immunized with this protein recognized proteins of 100 and 90 kDa as determined by immunoblotting. Confocal laser scanning microscopy and transmission electron microscopy of cells incubated first in the presence of the antibody and subsequently in the presence of fluorescein- or gold-labeled goat anti-rabbit IgG showed labeling of the protozoan surface as well as of some cytoplasmic vesicles.

The developments leading to the formation of the European Society for Animal Blood Group Research (ESABR) in Prague 1964

The developments leading to the formation of the European Society for Animal Blood Group Research (ESABR) in Prague 1964

List of Reagents for Animal Blood Group Research

List of Reagents for Animal Blood Group Research

List of Reagents for Animal Blood Group Research

List of Reagents for Animal Blood Group Research

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms

Defining the Specificity of Anti‐A/B IgM/G Antibodies with Different Antigens and Lectins1

The specificity of anti-histo blood group A/B antibodies is defined by the antigen used for their production and selection (in the case of mouse monoclonal antibodies, mAbs) or by the antigens present on various intestinal bacteria (for polyclonal human IgM and/or IgG, phAbs). Absorption experiments with red blood cells and free antigen have been used earlier to define specificity; here we use A/B substances of human, animal and synthetic origin as well as known reactivities of mAbs or lectins as tools to further characterize epitope properties of the A/B antigens. The signal response patterns obtained with identical phAbs anti-A/B IgM/G on three different antigens are superposable. In contrast, commercially available mAb anti-A/B IgM, when tested on the same antigens, revealed different response curves. The chemical specificity of lectins for distinct monosaccharides in terminal position was exploited to delineate the specificity of mouse ascites anti-A IgG1 and of phAbs anti-A/B IgM/G. Helix pomatia lectin inhibited the access of MB9 to porcine A almost completely whereas binding capacities of human anti-A IgM/G were inhibited by 50 and 62%, respectively. In similar experiments Bandeiraea simplicifolia lectin was seen to inhibit access of anti-B IgM/G to horse B by 34 and 58%, respectively. No inhibition was seen with lectin from Ulex europaeus or concanavalin A on plates coated with the three different A or B substances. Thin-layer chromatography (TLC) of antigens revealed one spot for the synthetic trisaccharides, whereas the human and animal blood group substances showed 2 and 3 spots, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

List of Contributions by the members on Animal Blood Groups and Protein Polymorphisms

List of Contributions by the members on Animal Blood Groups and Protein Polymorphisms

List of Reagents for Animal Blood Group Research

List of Reagents for Animal Blood Group Research

Defining the Specificity of Anti-A/B IgM/G Antibodies with Different Antigens and Lectins

The specificity of anti-histo blood group A/B antibodies is defined by the antigen used for their production and selection (in the case of mouse monoclonal antibodies, mAbs) or by the antigens present on various intestinal bacteria (for polyclonal human IgM and/or IgG, phAbs). Absorption experiments with red blood cells and free antigen have been used earlier to define specificity; here we use A/B substances of human, animal and synthetic origin as well as known reactivities of mAbs or lectins as tools to further characterize epitope properties of the A/B antigens. The signal response patterns obtained with identical phAbs anti-A/B IgM/G on three different antigens are superposable. In contrast, commercially available mAb anti-A/B IgM, when tested on the same antigens, revealed different response curves. The chemical specificity of lectins for distinct monosaccharides in terminal position was exploited to delineate the specificity of mouse ascites anti-A IgGl and of phAbs anti-A/B IgM/G. Helixpomatia lectin inhibited the access of MB9 to porcine A almost completely whereas binding capacities of human anti-A IgM/G were inhibited by 50 and 62%, respectively. In similar experiments Bandeiraea simplicifolia lectin was seen to inhibit access of anti-B IgM/G to horse B by 34 and 58%, respectively. No inhibition was seen with lectin from Ulex europaeus or concanavalin A on plates coated with the three different A or B substances. Thin-layer chromatography (TLC) of antigens revealed one spot for the synthetic trisaccharides, whereas the human and animal blood group substances showed 2 and 3 spots, respectively. Immunostaining experiments of TLC with mAbs revealed 1 spot only per lane, suggesting a high degree of epitope homogeneity.

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms

List of Reagents for Animal Blood Group Research

List of Reagents for Animal Blood Group Research

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms

21st International Conference on Animal Blood Groups and Biochemical Polymorphisms

21st International Conference on Animal Blood Groups and Biochemical Polymorphisms

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms

List of Reagents for Animal Blood Group Research

List of Reagents for Animal Blood Group Research

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms

List of Contributions by the Members on Animal Blood Groups and Protein Polymorphisms