Anatomical Dissection Research Articles

Anatomy and Histology of Sensorimotor Connections Between the Facial and Trigeminal Nerve in the Buccinator Muscle.

Despite indications of a close interaction between the trigeminal (CN V) and facial nerve (CN VII) within the buccinator muscle, a combination of anatomical dissection and histological analysis has not been reported. Five formalin-fixed and fresh-frozen hemifaces were dissected to reveal the buccal fat pad, the buccinator muscle, and anastomotic connections between CN V and CN VII within it. Samples were taken for histological processing and immunostaining. Branches of CN V and CN VII formed pronounced sensorimotor anastomotic connections in and surrounding the buccinator muscle. These findings were histologically evident with close intramuscular coupling of sensory and motor fibers. There was an evident but gradual shift from motor to sensory fibers in the interconnections when analyzing them from the side of CN V toward the side of CN VII and vice versa. These results further elucidate connections between CN V and CN VII and their possible role in proprioception of the facial muscles.

Integrating AI into Breast Reconstruction Surgery: Exploring Opportunities, Applications, and Challenges.

Background: Artificial intelligence (AI) has significantly influenced various sectors, including healthcare, by enhancing machine capabilities in assisting with human tasks. In surgical fields, where precision and timely decision-making are crucial, AI's integration could revolutionize clinical quality and health resource optimization. This study explores the current and future applications of AI technologies in reconstructive breast surgery, aiming for broader implementation. Methods: We conducted systematic reviews through PubMed, Web of Science, and Google Scholar using relevant keywords and MeSH terms. The focus was on the main AI subdisciplines: machine learning, computer vision, natural language processing, and robotics. This review includes studies discussing AI applications across preoperative, intraoperative, postoperative, and academic settings in breast plastic surgery. Results: AI is currently utilized preoperatively to predict surgical risks and outcomes, enhancing patient counseling and informed consent processes. During surgery, AI supports the identification of anatomical landmarks and dissection strategies and provides 3-dimensional visualizations. Robotic applications are promising for procedures like microsurgical anastomoses, flap harvesting, and dermal matrix anchoring. Postoperatively, AI predicts discharge times and customizes follow-up schedules, which improves resource allocation and patient management at home. Academically, AI offers personalized training feedback to surgical trainees and aids research in breast reconstruction. Despite these advancements, concerns regarding privacy, costs, and operational efficacy persist and are critically examined in this review. Conclusions: The application of AI in breast plastic and reconstructive surgery presents substantial benefits and diverse potentials. However, much remains to be explored and developed. This study aims to consolidate knowledge and encourage ongoing research and development within the field, thereby empowering the plastic surgery community to leverage AI technologies effectively and responsibly for advancing breast reconstruction surgery.

The Vascular Anatomical Basis for a Well-Designed Reconstruction of the Ala Nasi by a Microsurgical Preauricular Flap Technique

Background: A microsurgical auricular flap represents a single-step technique for the reconstruction of full-thickness defects of the ala nasi. To achieve the best surgical outcomes, it is essential to have an exhaustive knowledge of the vascular network to improve the management of the surgical flap. This study aimed to provide an anatomical and surgical guide for a well-designed reconstruction of the ala nasi using a free preauricular flap. Methods: In this study, three fresh-frozen and two formalin-fixed human head specimens injected with red silicone rubber to enhance the arterial facial system were used. The reconstruction of the full-thickness defect of the ala nasi was performed using a microsurgical auricular flap technique, with the dimensions of the ala nasi and the preauricular flap duly noted. In addition, anatomical dissections were conducted, during which the positions and diameters of the main donor and recipient vessels were measured. Results: A presurgical evaluation was performed to define the flap design. A comparison of the shape and mean dimensions of the ala nasi defect (height 9.66 ± 1.40 mm; thickness 3.52 ± 0.53 mm) and the preauricular flap (height 8.50 ± 2.68 mm; thickness 3.92 ± 1.29 mm) indicated that this flap was an optimal option for the reconstruction of the ala nasi. The surgical procedure involved the full-thickness removal of the ala nasi, and the harvesting and insertion of the preauricular flap. The anatomical measurements demonstrated that the facial artery and veins were the optimal recipient vessels, with a diameter of >1 mm (2.08 ± 0.56 and 2.85 ± 0.74 mm), suitable for anastomosis with the superficial temporal artery and vein (1.86 ± 0.58 and 1.66 ± 0.15 mm). In addition, the postsurgical evaluations indicated a slight mean difference in the thickness (1.14 ± 0.65 mm) and height (1.68 ± 1.18 mm) between the ala nasi and helix and a satisfactory VAS score (7.9 ± 0.57). Conclusions: Our surgical and anatomical data provide compelling evidence in favour of free preauricular flap reconstructions of the ala nasi. This procedure allows for the correct choice of recipient vessels and the creation of a well-designed surgical flap.

The frontal aslant tract: Anatomical description and case report

Background: Advances in surgical techniques, neuroimaging, and white matter fiber dissection have facilitated the identification of critical tracts like the frontal aslant tract (FAT) that have garnered attention, despite remaining poorly recognized within the neurosurgical community. Case Description: We report the case of a 37-year-old male right-handed patient presenting with headache and epilepsy, in whom neuroimaging revealed an intra-axial lesion in the left middle frontal gyrus closely associated with FAT. Successful navigation-guided resection of the lesion was achieved, resulting in a favorable neurological outcome attributable to the preservation of the tract. This case is complemented by a review of the literature and anatomical dissection of FAT in a human specimen. Conclusion: The FAT has emerged as a critical white matter structure in neurosurgery, given its involvement in speech and motor functions. This case demonstrates the importance of advanced imaging modalities and intraoperative technologies to ensure safe resection.

Quantitative Analysis of Genicular Nerve Block Spread and Variability: Anatomical Correlations and Clinical Implications

BackgroundDespite advancements in anaesthesiology and regional anaesthesia, the three-dimensional spread of local anaesthetics is still poorly understood. This study investigates the pericapsular spread of radiopaque contrast after ultrasound-guided genicular nerve block in cadaveric knees using CT reconstruction. The aim is to assess the reproducibility, accuracy, and variability of this technique in order to improve safety and effectiveness. MethodsWe used a four-stage methodology that involved performing genicular nerve block on cadaveric knees, acquiring data using high-resolution ultrasound and helical CT imaging, performing segmentation and surface reconstruction of the CT images, and analysing the data quantitively to determine the spread of the infiltrate in each genicular nerve region. Coefficients of variation were calculated to estimate the spread and reproducibility of each genicular nerve block. ResultsOur results revealed significant variability in spread in all genicular nerve blocks, particularly around the recurrent genicular nerve. Despite this variability, the spread—particularly along the medial-lateral and anterior-posterior axes—showed moderate consistency. Furthermore, the regions covered by 80% of the spread closely matched the pericapsular distribution of the genicular nerves of the knee. ConclusionsNotable variability in spread was observed in genicular nerve blocks, particularly around the recurrent genicular nerve. However, the spread showed moderate consistency and aligns closely with the pericapsular nerve distribution of the knee. Future studies should combine quantitative analysis with anatomical dissection to further investigate the involvement of the deep peroneal motor branch.

Radiology during a war - experience in Ukraine.

Military radiology, an integral part of military medicine, plays a pivotal role in medical triage, diagnosis, and treatment. Its significance lies in providing timely and accurate assessments in challenging situations.The utilization of contemporary sonographic techniques enables rapid identification of life-threatening conditions, ensuring prompt medical aid and facilitating regional anesthesia. Computed tomography emerges as a critical tool for assessing injury extent, planning surgeries, monitoring postoperative phases, and conducting retrospective evaluations, especially when anatomical dissection is complex.Battlefield radiology not only enhances the understanding of injury mechanisms and battlefield traumas but also contributes significantly to the overall improvement of diagnostic and treatment approaches. Ukrainian doctors actively engaged in diverse stages of patient care accumulate a wealth of knowledge, substantially elevating the survival rates of wounded individuals. This experience serves as the foundation for ongoing enhancements and the advancement of military radiology, even during periods of peace. · Military radiology is essential in medical triage, diagnosis, and treatment within military contexts.. · Modern sonographic methods enable swift identification of life-threatening conditions.. · Computed tomography is indispensable for assessing injuries, planning surgeries, and conducting retrospective evaluations.. · Ukrainian doctors actively contribute to the knowledge base, improving diagnostic and treatment practices.. · The acquired experience serves as a foundation for ongoing advancements in military radiology, extending its impact beyond wartime scenarios.. · Nehria N, Nehria Y, Bukharin T. Radiology during a war - experience in Ukraine. Fortschr Röntgenstr 2024; DOI 10.1055/a-2326-7724.

Perception of First-Year MBBS Students Toward Virtual Dissection in Learning Anatomy: A Comparative Study Between High and Low Academic Achievers

Perception of First-Year MBBS Students Toward Virtual Dissection in Learning Anatomy: A Comparative Study Between High and Low Academic Achievers

A Novel Ultrasound-Guided Cervical Plexus Block: A Cadaveric Canine Study.

The ultrasound-guided cervical plexus plane (US-CPP) block has proven effective in humans; yet its application in dogs remains unexplored. This study aimed to describe a novel US-CPP approach in canines. A local sonoanatomy was mapped, the injection technique was tested, and a gross anatomical dissection (GAD) was performed on one cadaver. The bilateral injectate spread and nerve staining were then evaluated via a CT scan and GAD in the six cadavers. The transducer was aligned parallel to the cervical spine, caudal to the atlas. After identifying the cleidocervical and omotransversarius muscles and the C2-C3 interfascial plane, a spinal needle was inserted in-plane, and 0.15 mL/kg of a dye-contrast solution was injected. CT imaging showed the contrast reaching the C1, C2, and C3 vertebral bodies in 3 out of 12 (3/12), 11/12, and 12/12 injections, respectively, and the C4, C5, and C6 vertebrae in 8/12, 5/12, and 1/12 injections, respectively. No contrast was detected in the epidural space. The C1, C2, C3, C4, and C5 nerves were stained in 3/12, 10/12, 8/12, 2/12, and 0/12 injections, respectively. No significant differences were observed between the groups (p > 0.05). A single US-CPP injection successfully stained the C2 and C3 nerves, indicating the potential clinical applicability, although the injected volume rarely reached C4 and C5.

Morphological characteristics of rabbits’ carpal tunnel: Evaluation of its potential for carpal tunnel research

This study aimed to define the main anatomic components and describe the normal size and form of the carpal tunnel in rabbits using imaging techniques. It was also aimed to evaluate the potential of rabbits as an animal model for carpal tunnel research. The forelimbs of eight adult New Zealand rabbits were investigated using histology, magnetic resonance, and computed tomography. Two cadavers were used for anatomical dissection to support the findings. The carpal tunnel was examined at proximal and distal levels, and it was found that the flexor retinaculum is two-layered in rabbits. Both vascular nerve bundles, including the median nerve and the ramus palmaris of the ulnar nerve, were within two layers of it and passed through the carpal tunnel. The deep digital flexor, superficial digital flexor, and radial carpal flexor tendons were observed within the carpal tunnel. The flexor pollicis longus tendon is not found in rabbits. The area of the carpal tunnel narrowed towards the distal end in three methods, and its depth decreased in CT and MRI. The length of the accessory carpal bone determined this depth. The study found that rabbits' carpal tunnels are morphologically similar to those of humans compared to dogs. This situation is thought to pave the way for more accurate approaches to carpal tunnel research in rabbits.

Midclavicle block: An anatomical study.

Recent studies indicate that clavipectoral fascia plane block (CPB) efficacy may stem from injectate distribution to the anterosuperior clavicular periosteum. We conducted an anatomical study combining the CPB with injection within the subclavius muscle. Our hypothesis was that the anaesthetic injectate would fully cover both the anterosuperior and posteroinferior surfaces of the clavicular periosteum in the midclavicular region. Observational human cadaver study. Laboratory of Surgical Neuroanatomy, Unit of Anatomy and Human Embryology of the Faculty of Medicine and Health Sciences, University of Barcelona. Five fresh human cadavers. The cadavers received both a CPB and subclavius muscle injections under ultrasound guidance in 10 clavicular regions. After the procedures on the cadaveric models, an anatomical dissection by planes was performed to evaluate the distribution pattern of methylene blue on the clavicular periosteum. A probabilistic map of the colour spectrum and staining temperature on the clavicular surfaces was generated. Methylene blue stained 37 ± 16% of the anterosuperior surface and 23 ± 13% of the posteroinferior surface of the clavicular periosteum, particularly in the middle third of each surface. Although the staining did not achieve complete circumferential coverage or perfect alignment between the surfaces, the areas exhibited a close relationship, indicating significant distribution and relevant coverage. Our anatomical study demonstrates that the midclavicular block achieves effective distribution around the middle third of the clavicle, although complete circumferential anaesthesia of the clavicular periosteum was not achieved. Although this block may provide periosteal and bone anaesthesia, it does not address other sources of pain, such as muscle spasms and skin components. Additional clinical studies are needed to evaluate the overall efficacy of this dual block technique for clavicle surgery.

An ultrastructure analysis of the developing human anterior cruciate ligament tibial enthesis.

This study aimed to investigate the ultrastructural anatomy of the developing ACL tibial enthesis. We hypothesized that enthesis architecture would progressively mature and remodel, eventually resembling that of the adult by the early postnatal stage. Five fresh-frozen human pediatric cadaveric knees aged 1-36 months underwent anatomical dissection to harvest the ACL insertion and underlying tibial chondroepiphysis. The samples were prepared for scanning electron microscopy (SEM) to examine the ultrastructural anatomy of the enthesis and underwent histological staining for circular polarized light (CPL) and light microscopy imaging. SEM analysis of the 1- and 8-month-old samples revealed a shallow interdigitation between the dense fibrous (ligamentous) tissue and unmineralized chondrogenic tissues, with a minimal transition zone. By 11-month, a more complex transition zone was present. By age 19- and 36-month-old, a progressively more complex and defined fibrocartilage zone was observed. CPL analysis revealed distinct collagen fiber continuity, alignment, and organization changes over time. By 19 and 36 months, the samples exhibited complex fiber arrangements and a progression toward uniform fiber orientation. Similarly, histological analysis demonstrated progressive remodeling of the enthesis with increasing age. Our results suggest that the ACL enthesis of the developing knee begins to mimic that of an adult as early as 19 months of age, as a more complex transition between ligamentous and chondro-epiphyseal tissue can be appreciated. We hypothesize that the observed changes are likely due to mechanical loading of the enthesis with the onset of weightbearing. Future investigations of ACL reconstruction and repair will benefit from improved understanding of the chondro-epiphyseal/ACL regions.

Quantitative anatomy of the infraspinatus muscle in the human fetus.

The study presents one of the six scapulohumeral muscles, which occupies most of the osteofibrous infraspinatus compartment. Along with the supraspinatus, teres minor and subscapularis muscles, the infraspinatus muscle contributes to the rotator cuff. It protects the posterior aspect of the articular capsule of the shoulder joint, adducts and externally rotates the arm. The aim of the study was to perform the quantitative analysis of the infraspinatus muscle in human fetuses and to elaborate growth dynamics for its morphometric parameters. Using anatomical dissection, digital image analysis (NIS Elements AR3.0) and statistics (Student's t-test, regression analysis), the vertical, transverse and oblique diameters, muscle circumference and projection surface area of the infraspinatus muscle were measured in 36human fetuses of both sexes (17♂, 19♀) aged 18-30weeks. The infraspinatus muscle revealed neither sex nor laterality differences. All examined morphometric parameters of the infraspinatus muscle increased commensurately in accordance with the following linear functions: y=-4.024 + 0.903×Age ± 0.621 (R²=0.96) for transverse diameter, y=-3.089 + 1.321×Age ± 0.897 (R²=0.97) for vertical diameter, y=-1.161 + 0.632×Age ± 0.444 (R²=0.97) for oblique diameter, y=-13.575 + 3.851×Age ± 1.938 (R²=0.98) for muscle circumference and y=-293.512 + 23.228×Age ±19.650 (R²=0.95) for projection surface area.

Variation and Arrangement of the Digastric Muscle in a Chinese Population.

The digastric, a prominent anatomic feature of the head and neck, exhibits clear ethnographic differences in the incidence of variation in its anterior belly (AB) and the relationship of its intermediate tendon (IT) to the stylohyoid (SH). There is a limited amount of information available regarding the digastric in the Chinese population, with most descriptions being individual cases. The present study aims to investigate the variability of AB, elucidate the relationship between IT and SH, and further address the arrangement of individual digastric muscles according to the type of AB and posterior belly (PB) of the digastric, as well as IT, in a Chinese population. Anatomic dissections of the head and neck were carried out bilaterally after student-performed neck dissections during a topographic anatomy course. The types of AB, PB, and IT were recorded to characterize and classify each digastric muscle. The incidence of AB variation was 21.05%. IT type Ⅰ and IT type Ⅱ accounted for 31.25% and 68.75%, respectively. The gross anatomy of the digastric was dominated by the arrangement of AB type I, IT type Ⅱ, and PB type I (63.16%). In addition, 2 rare AB variations were identified. To the best of our knowledge, this is the first report describing individual digastric arrangements and anatomically defining the normal digastric arrangement in the Chinese population. Careful consideration of the variant and anomaly of the digastric is critical for both surgeons and radiologists to provide accurate diagnostic and therapeutic procedures.

Can indoor plants reduce formaldehyde levels in the anatomy dissection hall? A study to evaluate the practicality of using plants in reducing formaldehyde levels

Background Formaldehyde a commonly used embalming fluid for the preservation of cadavers, produces numerous short and long-term side effects on the students and staff working with it. Indoor plants have been shown to reduce atmospheric formaldehyde levels. The purpose of the study was to compare the efficacy of indoor plants in reducing formaldehyde levels in ambient air to levels deemed safe by WHO standards Methods The study was conducted in the storage room of the Anatomical dissection hall. 7 potted plants of one variety were kept in the storage room for 14 days and the Formaldehyde reading was measured using a VOC (Volatile Organic Compound) monitor. Then the same was done with no plant. This cycle was repeated 2 more times, with two different plant species. The three spieces of plants used for this study were as follows: Dracaena trifasciata, Epipremnum aureum, Spathiphyllum. Results The results were analysed and compared and it was determined that the effect of having a plant present versus having no plant present on the Formaldehyde concentration in the air was inconclusive as Dracaena trifasciata failed to reject the null hypothesis altogether (p-value>0.05), while Epipremnum aureum and Spathiphyllum rejected the null hypothesis (p-value<0.05), however they both had a weak positive correlation with formaldehyde concentration. Conclusion The efficacy of indoor plants in reducing formaldehyde levels in ambient air needs to be further explored and validated since all the prior studies conducted were in controlled environment and should be done in a real time scenario for its practical and beneficial uses.

Ultrasound-Guided Deep Serratus Plane Block in Cat Cadavers (Felis catus): A Description of Dye and Contrast Media Distribution.

The serratus plane block is an ultrasound-guided anaesthetic technique that aims to provide analgesia to the lateral thoracic wall cranial to the 8th rib. This block can be performed in a superficial (between the latissimus dorsi and the serratus ventralis thoracis (SVT) muscles) or deep plane (between the intercostales externi and the SVT muscles). This study aimed to assess the distribution and nerve staining of a mixture of 0.4 mL kg-1 of methylene blue and iopromide 50:50 performing a deep serratus plane (DSP) block at the level of the 5th rib. We hypothesise that this technique would be feasible and could stain the rami cutanei laterales (RCL) of the intercostales nerves cranial to the 8th rib in cat cadavers. This study was divided into two phases. Phase 1 consisted of an anatomical study of the thoracic wall (2 cadavers). Phase 2 consisted of the ultrasound-guided injection of the aforementioned mixture and the assessment of its distribution by computed tomography and anatomical dissection (12 cadavers). Contrast media spread a median of 5.5 (2.5-7.5) intercostal spaces. The dye stained a median of 3 (0-5) RCL, affecting RCL 2 (17.39%), RCL 3 (57.17%), RCL 4 (78.26%), RCL 5 (91.30%), RCL 6 (78.26%), and RCL 7 (8.70%) within the DSP. Occasionally, the rami dorsales laterales and the thoracicus longus nerve were stained. Based on these findings, the DSP block performed with a volume of 0.4 mL kg-1 of an anaesthetic could provide analgesia in the area innervated from T4 to T6.

A Scoping Review on the Utility of Ultrasound to Visualize Bursae in Anatomical Dissection Courses

Bursitis is a common condition in clinical practice, often causing pain in the shoulder and buttock areas due to inflamed bursae. Proper diagnosis and treatment depend on knowing the presence and exact location of these bursae. Anatomy classes typically provide limited instruction on bursae because they are difficult to demonstrate during dissection courses. High-resolution ultrasound is an essential and versatile technique for detecting bursitis, and it could also serve as a valuable tool for students to better understand bursae. Relevant studies were screened in the following databases: CENTRAL, MEDLINE, BIOSIS Previews, EMBASE, and Web of Science Core Collection. Grey literature was also considered. Literature was screened on January 3, 2023. Only ultrasound investigations in human cadaver bursae were included, specifically using B-Mode ultrasound. The general characteristics of the included studies and the ultrasound-guided approaches for labeling the bursae were analyzed and examined. T The search found 8,899 matches, but only 15 met the criteria. Fifteen different bursae were studied, and 12 studies were included in the analysis. Both the marking substrate and the injected volume varied. Despite a high overall accuracy of 99% achieved using ultrasound-guided labeling approaches in the included studies, caution is advised due to the small sample size (1 to 24). The current study serves as a review to examine ultrasound studies on bursae in human cadavers. Ultrasound-guided labeling techniques achieve high accuracy and could be a valuable teaching tool in dissection courses. These techniques help visualize difficult-to-dissect structures and provide students with an understanding of sonoanatomy.

Bridging Innovation and Education: IJMS Volume 12 Issue 3 and the 2024 World Conference on Medical Student Research (WCMSR)

The International Journal of Medical Students (IJMS) is proud to present Volume 12, Issue 3, highlighting innovative research across various medical disciplines. This issue underscores the journal's commitment to fostering medical education and advancing healthcare. Featured articles explore critical areas, including narrative medicine's role in reducing burnout, stress management among medical students, and the long-term impacts of the COVID-19 pandemic on cancer referrals and treatment decisions. Public health concerns such as antimicrobial resistance and access to syphilis screening in vulnerable populations are also examined. Additionally, this edition includes insightful reviews on pediatric allergy consultations and the utility of ultrasound in anatomical dissection courses. Case reports on rare medical conditions, reflections on global surgical electives, and personal experiences in overcoming research challenges further enrich the content. To complement this issue, the IJMS announces the 2024 World Conference on Medical Student Research (WCMSR), taking place on October 26, 2024, with participants from over 40 countries. This event promises to be a valuable platform for showcasing the best global research, fostering discussion, and inspiring the next generation of medical professionals.

Expression of Genes Related to Meat Productivity, Metabolic and Morphological Significance of Broiler Chickens with the Use of Nutritional Phytochemicals.

The study aimed to analyze gene expression linked to skeletal muscle growth and lipid metabolism in broiler chickens fed with plant extracts. Five groups of chickens were formed: four experimental groups and one control group. The diets of the experimental groups were supplemented with different plant extracts: chicory, St. John's wort, maral root, and creeping thyme, whereas the control group received feed without phytobiotic compounds. Weekly weighings were conducted (n = 36). The chickens were slaughtered at day 26 for tissue sampling of four birds from each group. Gene expression (MYOG, MSTN, FASN) related to muscle growth and fatty acid synthesis was analyzed using the β-actin ACTB gene as a reference. Blood samples were taken at day 35 for biochemical analysis and anatomical dissection was performed. The study revealed that using plant extracts from chicory, thyme, and maral root increased MYOG gene activity by 4.21, 7.45, and 8.93 times, respectively. T. serpyllum extract boosted the MSTN gene by 10.93 times, impacting muscle growth regulation. FASN gene expression for fatty acid synthesis increased significantly by 18.22-184.12 times with plant extracts. The best results regarding meat productivity of chickens were obtained when using R. carthamoides extract. The results of the study will serve as a basis for further development of a phytocomposition designed to increase the meat productivity of broiler chickens in the production of environmentally safe poultry products.

Simulating Upper Eyelid Ptosis During Neuromodulator Injections-An Exploratory Injection and Dissection Study.

Aesthetic neuromodulator injections of the upper face are frequently performed to temporarily block muscular actions of the periorbital muscles to ultimately reduce skin rhytids. However, the adverse event rate in the literature for toxin-induced blepharoptosis ranges from 0.51% to 5.4%. To identify access pathways by which injected neuromodulator product can travel from extra- to intra-orbital and therefore affect the levator palpebrae superioris muscle. Nine non-embalmed human body donors were investigated in this study with a mean age at death of 72.8 (16.1) years. The 18 supraorbital regions were injected in 28 times (14 for supratrochlear and 14 for supraorbital) with 0.5 cc, whereas eight cases (four for supratrochlear and four supraorbital) were injected with 0.1 cc of colored product. Anatomic dissections were conducted to identify structures stained by the injected color. The results of this injection- and dissection-based study revealed that both the supratrochlear and the supraorbital neurovascular bundles are access pathways for injected neuromodulator products to reach the intra-orbital space and affect the levator palpebrea superioris muscle. Out of 36 conducted injection passes, seven (19.44%) resulted in affection of the sole elevator of the eyelid of which 100% occurred only at an injection volume of 0.5 cc and not at 0.1 cc. Clinically, the results indicate that a low injection volume, a superficial injection for the supraorbital location, and angling the needle tip away from the supratrochlear foramen (toward the contralateral temple) when targeting the corrugator supercilii muscles, can increase the safety profile of an aesthetic toxin glabellar treatment.

Topography of the urinary organs in the Baikal seal

The urinary system of aquatic mammals plays an important role in maintaining the body’s homeostasis. Features in the structure and topography of the urinary organs are associated with the habitat and adaptation of the organism of aquatic animals to it. Interest in studying the features of the topography of organs and the structure of the organism as a whole, as well as the organs of urine formation and excretion in particular, is presented by the Baikal seal, given its freshwater lifestyle. The goal was to describe the species-specific features of the topography of the kidneys, ureters, bladder and urethra in male and female Baikal seals. The object of the study was both live individuals and carcasses of the Baikal seal (Phoca sibirica) aged from 1.5 to 3 years. The animals were examined at the Baikal Seal Aquarium LLC and the Irkutsk City Animal Disease Control Station. The cadaver material was collected under the program of the Russian Federal Agency for Fisheries, as well as research and development, experimental design and technological developments (R&D and R&D) from 2014, 2017, 2020. The study material was the organs of the urinary system: kidneys, ureters, bladder, urethra. The studies were carried out using various anatomical methods: autopsy of cadaver material with examination and fixation of syntopy, skeletotopy, holotopy and anatomical dissection, ultrasound diagnostics and multispiral computed tomography.