Desorption Of Analytes Research Articles

How do cocaine and morphine in methanol solution desorb at the last moment of Leidenfrost phenomenon-assisted thermal desorption?

The objective of the present study is to investigate desorption of low-volatility analytes in Leidenfrost phenomenon-assisted thermal desorption (LPTD). LPTD was investigated for 5μL solutions of 0.03 ppm cocaine or morphine in methanol (sample weight: 0.12 ng) by using heated metal surfaces (240°C) polished by abrasives with grit numbers from #5000 (~3μm) to #100 (~200 μm). The analyte signals were detected only after the complete evaporation of methanol solvent and the formed analyte residues levitated on the heater surface. The strongest ion signals were obtained with grit number #100. Because the analyte residue does not come into contact with the heated surface but levitates on the hot substrate after the evaporation of the solvent, thermal decomposition of the analyte is largely suppressed. This is a great merit of LPTD for trace analysis of low-volatility and thermally labile compounds.

Nutshell Materials as a Potential Eco-Friendly Biosorbent for the Effective Extraction of UV Filters and Parabens from Water Samples.

UV filters and parabens, as ingredients of cosmetics, are commonly occurring water pollutants. In our work, nutshells were used as biosorbents in the developed analytical procedure for the determination of UV filters and parabens in water samples. The shells obtained from walnuts, hazelnuts, peanuts and pistachios were applied as biosorbents. The proposed analytical method can be used as a powerful alternative to other methods for the analysis of UV filters and parabens in water samples. A method of carrying out the sorption step and its parameters, i.e., the effect of time, pH, and salt addition, was developed. A method for the desorption of analytes was also developed, in which the type and volume of solvent, and the desorption time, were established. The recoveries were in the range of 59-117% for benzophenones and lower recoveries from 14 to 75% for parabens. The results showed that nutshells can be used as low-cost, efficient and eco-friendly biosorbents for the determination of parabens and UV filters in water samples. These materials can be used as a 'greener' replacement for the commercially available adsorbents for the extraction of cosmetic ingredients from the environment.

Determination of Nitrobenzenes in Air by a Needle Trap Device (NTD) and Gas Chromatography-Mass Spectrometry (GC-MS)

A simple needle-trap device (NTD) coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine trace levels of nitrobenzenes (NBs) in workplace air. The NTD with sorbent inside the needle was employed for the quantification of nitrobenzene, o-nitrotoluene, m-nitrotoluene, p-nitrotoluene, o-nitrochlorobenzene, m-nitrochlorobenzene, and p-nitrochlorobenzene. The optimum temperature and time for desorption of analytes were 270 °C and 4 min, and parameters such as sampling flow rate, breakthrough volume, and storage capability were also optimized. The limits of detection and quantification were in the ranges of 1.8 × 10−4–1.1 × 10−3 and 5.9 × 10−4–3.6 × 10−3 mg m−3, respectively. The developed protocol was successfully applied for the determination of NBs in vehicle repair plants where the highest detected concentration was 0.11 mg m−3. The established NTD-GC-MS generally applies to monitoring trace levels of NBs in the air.

Investigation of the laser fluence and wavelength dependence in surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles.

Surface-assisted laser desorption/ionization (SALDI) mass spectrometry (MS) builds on the use of nanostructured surfaces (e.g., coatings of colloidal nanoparticles) to promote analyte desorption and ionization. The SALDI process is believed to occur mainly through thermal processes, resulting from heating of the nanosubstrate upon absorption of the photon energy, and by assisting ionization steps. Mostly due to the accessibility of the respective hardware, the majority of SALDI-MS studies use standard laser wavelengths for MALDI (i.e., 337 or 355 nm), even though peak absorption of the SALDI nanosubstrate might completely differ from these values. Here, we investigated the wavelength dependence in SALDI-MS to determine if wavelength adjustment would be beneficial, and to provide new experimental data for a better understanding of the SALDI mechanism. To this end, gold nanoparticles (AuNPs) sprayed onto microscope glass slides were employed as SALDI nanosubstrates and L-arginine as a model analyte. In addition, we used 2,5-dihydroxyacetophenone (2,5-DHAP) for classical MALDI-MS using the same experimental setup. Arginine ion signals were recorded as a function of laser wavelength and laser fluence. Mass spectra were acquired in the wavelength range between 310 and 630 nm, including the absorption maximum of the sprayed AuNPs around 550 nm and that of 2,5-DHAP around 380 nm. Laser fluence thresholds for the generation of arginine ions were found to be dependent on the laser wavelength and to inversely correlate with the absorbance profiles of the deposited AuNPs and 2,5-DHAP, respectively. Very differently to MALDI, in SALDI ionization efficiency was found to strictly linearly decrease with increasing laser wavelength. Our results, therefore, corroborate the general assumption that material ejection in SALDI-MS is mainly driven by thermal processes in the low laser fluence range and add new evidence that the ionization process is directly influenced by photon energy when AuNPs are employed as nanosubstrates.

Analysis of nitrogen-containing rocket fuel transformation products in loamy soil by thermal desorption gas chromatography-tandem mass spectrometry.

Unsymmetrical dimethylhydrazine (UDMH) based rocket fuel, when released into the soil, undergoes oxidative transformations to form a variety of toxic nitrogen-containing products (TPs). Loamy soils containing aluminosilicates (clay) are capable of strong binding and retention of UDMH TPs due to a combination of polar sorption and cation-exchange properties, posing challenges for their extraction and quantification. To overcome this problem, the present study proposes direct thermal desorption (TD) of analytes from loam facilitated by the addition of modifiers competing with UDMH TPs for sorption centers and ensuring their conversion into molecular form. Among tested additives, the mixture of potassium chloride and hydroxide demonstrated the best performance and provided recoveries of the most UDMH TPs exceeding 70% under optimized TD conditions (200°C, 30min). The online combination of TD with gas chromatography-tandem mass spectrometry allowed for the development of a method for the determination of 15 UDMH TPs in loamy soils with limits of detection in the range of 0.2-15µg/kg. The use of matrix-matched calibration and deuterated internal standards ensured high accuracy (80%-100%) and precision (relative standard deviation<18%) of the analysis. The developed method was validated and successfully tested in the analyses of real loamy soil samples polluted with rocket fuel.

A fast thermal desorption unit for micro thermal desorption tubes, Part I: Development of the system and proof of concept measurements with hyper-fast gas chromatography

A system consisting of a thermal desorption unit (TDU) and micro thermal desorption tubes (μTD-tubes, 1.4 mm I.D., 10mg Tenax TA) for fast desorption of analytes was developed for the efficient combination of hyper fast gas chromatography with thermal desorption. The fast desorption is achieved by a significantly reduced thermal mass compared to conventional thermal desorption tubes. Therefore, extremely fast heating and cooling cycles are possible. Proof of concept measurements combining the new setup with a flow-field thermal gradient gas chromatograph (FF-TG-GC) and FID detection show good precision and linearity with R2≥0.995 in the analysis of an n-alkane mix (C8-C20). Thermal desorption occurs within 12s. The impact of reduced μTD-tube dimensions on desorption time, full width at half maximum (FWHM), breakthrough volumes, tube flow rates ergo linear velocities, porosity and back pressure is discussed.

Development, Demonstration, and Evaluation of Routine Monitoring of Aerosol Carbon, Oxygen, and Sulfur Content.

Traditional online measurements of the chemical composition of particulate matter have relied on expensive and complex research-grade instrumentation based on mass spectrometry and/or chromatography. However, routine monitoring requires lower-cost alternatives that can be operated autonomously, and such tools are lacking. Routine monitoring of particulate matter, especially organic aerosol, relies instead on offline techniques such as filter collection that require significant operator effort. To address this gap, we present here a new online instrument, the "ChemSpot", that provides information on organic aerosol mass loading, volatility, and degree of oxygenation, along with sulfur content. The instrument grows particles with water condensation, impacts them onto a passivated surface with low heat capacity, and uses stepped thermal desorption of analytes to a combination of flame ionization detector (FID) and flame photometric detector (FPD) and then to a CO2 detector downstream of the FID/FPD setup. By relying on detectors designed for gas chromatography, calibration is achieved almost entirely through the introduction of gases without the need for regular introduction of particle-phase calibrants. Particle collection efficiency of greater than 95% was achieved consistently, and the collection cell was shown to rapidly and precisely heat to ∼800 °C at a rate as fast as 10 °C per second. Measurements of total organic carbon, volatility distribution of organic aerosol, total sulfur, and oxygen-to-carbon ratio (O:C) collected during a continuous multi-week period are presented here to demonstrate the autonomous operation of "ChemSpot". Colocated measurements with a mass spectrometer, an aerosol chemical speciation monitor (ACSM), show good correlation and relatively low bias between the instruments (mean absolute percentage error of 21% and 27% for organic carbon and equivalent sulfate measurements, respectively).

Development of Dry-herb vaporizer-assisted solid-phase Microextraction for the analysis of volatile Organic compounds

Solid-phase microextraction (SPME) is a simple, efficient, and solvent-free technique. It integrates the steps of traditional liquid-liquid extraction, i.e., sampling, extraction, concentration, derivatization, in a single step. It is easy, combining capability with gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS), which makes this method fast, reliable, and sensitive. SPME method is often associated with extraction at elevated temperature, since increasing temperature may positively affect extraction by increasing the analyte volatility, diffusion, and extraction rate. In this work, a dry-herb vaporizer (vaping pen) was used as a sample heating device for direct desorption of volatile analytes into the headspace of the device. The extraction was performed by sorbing analytes from their gas phase in the headspace of the vape onto a SPME fiber followed by desorption in the injection port for GC–MS characterization. Compounds from horseradish, cinnamon, and gasoline-spiked soil samples were analyzed. The samples were also analyzed by conventional headspace SPME-GC–MS. The results from both methods were compared. The methods produce comparable results in terms of extracted compounds and relative area percentages, however, the dry-herb vaporizer had improved sensitivity.

Zn-doped MoO3 nanorods for dispersive solid phase extraction of trace Pb(II) from water and food samples

A rapid, and environmentally friendly dispersive solid phase extraction method based on Zn-doped MoO3 nanorods for efficient extraction of trace level of Pb(II) from water and food samples was established before high-resolution continuum source flame atomic absorption spectrometric detection. The sorbent characterization studies were carried out using BET, FE-SEM, XRD, and FT-IR techniques. To achieve effective phase separation and accurate analytical data, the adsorption and desorption procedures have been precisely adjusted. The presented approach demonstrated a wide working range (9.3–500 µg L−1), a relatively low detection limit (3.1 µg L−1), and very good extraction recoveries (90–104%) under optimum conditions. The matrix effect was investigated by the addition of interference ions. The presence of many different anions and cations in the environment did not lead to negative results and selectivity towards the analyte was observed. The method was validated by the analysis of certified reference materials (TMDA - 64.3 and INCT-OBTL-5). The enhancement factor has been determined as 10.9. The suggested method worked efficiently to extract and determine Pb(II) from water, tea, and oil samples.

Modeling the ionization mechanism of amorphous solid particles without an external energy source coupled to mass spectrometry.

Ionization via desorption of charged analytes from the surface of solid amorphous glutaric acid particles, without the assistance of an external energy source, has been shown to be a promising method that can be coupled to mass spectrometry. We conduct mechanistic studies of the later stages of this ionization process using atomistic molecular dynamics. Our analysis focuses on the hydrogen bonding, diffusion, and ion desorption from nano-aggregates of glutaric acid. These nano-aggregates exhibit an extended H-bonded network, often comprising H-bonded chains, linear dimeric assemblies, and occasionally cyclic trimeric assemblies. These local structures serve as centers for proton transfer reactions. The intermediate hydrocarbon chain between the proton-carrying oxygen sites prevents proton diffusion over a long distance unless there is significant translational or rotational movement of the proton-carrying diacid molecule. Our calculations show that diffusion on the surface is an order of magnitude faster than in the core of the nano-aggregate, which aids effective proton transfer on the particle's exterior. We find that ionic species desorb from the aggregate's surface through independent evaporation events of small clusters, where the ion is coordinated by only a few glutaric acid molecules. Near the nano-aggregate's Rayleigh limit, jets capable of releasing multiple ions were not observed. These observations suggest a more general ion-evaporation mechanism that applies to low-dielectric particles of various sizes, complementing the original ion-evaporation mechanism proposed for aqueous droplets with an approximate radius of 10-15 nm. The combined evidence from molecular modeling presented here and the thermodynamic properties of solid and supercooled liquid glutaric acid indicates that the stronger signals of glutaric acid observed in the mass spectra, relative to other experimentally tested diacids, can be attributed to its significantly lower melting point and the reduced enthalpy of vaporization of its amorphous state compared to other tested diacids.

Simple and convenient preconcentration procedure for the isotopic analysis of uranium in seawater.

The demand for monitoring anthropogenic U isotopes, 236U and 233U, in seawater will continue to increase due to radioecological issues and the need for tools for environmental dynamics research. In response to this growing demand, herein, a novel and simple method was developed for the collection of U isotopes in seawater, both in the laboratory and field, using a fabric-like amidoxime adsorbent. The results from the adsorption studies showed that the optimum conditions for processing seawater in a glass beaker were as follows: seawater pH 4, amidoxime adsorbent 0.20 mmol per 500 g seawater and an adsorption time of 9 hours. Alternatively, when using a closed polyethylene container in experiments on-board a ship and using the same ratio of adsorbent to seawater as in the beaker experiment in the laboratory, the optimum conditions were as follows: seawater pH 8 and an adsorption time of 24 hours. Under the above-mentioned conditions, more than 95% of the U underwent adsorption in both the beaker and the polyethylene container experiments. In the case of analyte desorption, more than 80% of U in seawater was recovered using 2-3 mol dm-3 HCl or HNO3 as the eluent. Thus, it was concluded that the amidoxime adsorbent can serve as a simple and effective pre-concentration method for the ultra-trace monitoring of U isotopes in seawater.

Integrating Post-Ionization Separation via Differential Mobility Spectrometry into Direct Analysis in Real Time Mass Spectrometry for Toy Safety Screening.

Direct analysis in real time (DART) enables direct desorption and ionization of analytes, bypassing the time-consuming chromatographic separation traditionally required for mass spectrometry (MS) analysis. However, DART-MS suffers from matrix interference of complex samples, resulting in compromised detection sensitivity and quantitation accuracy. In this study, DART-MS was combined with differential mobility spectrometry (DMS) to provide an additional dimension of post-ionization ion mobility separation within a millisecond time scale, compensating for the lack of separation in DART-MS analysis. As proof-of-concept, primary aromatic amines (PAAs), a class of potentially hazardous chemicals, were analyzed in various toy products, including bubble solutions, finger paints, and plush toys. In addition to commercial Dip-it glass rod and metal mesh sampling tools, a customized rapid extractive evaporation device was designed for the accelerated extraction and sensitive analysis of solid toy samples. The incorporation of DMS in DART-MS analysis enabled the rapid separation and differentiation of isomeric analytes, leading to improved accuracy and reliability. The developed protocols were optimized and validated, achieving good linearity with correlation coefficients greater than 0.99 and acceptable repeatability with relative standard deviations less than 10%. Moreover, satisfactory sensitivity was realized with limits of detection and quantitation ranges of 0.2-5 and 1-20 μg/kg (μg/L) for the 11 PAA analytes. The established methodology was applied for the analysis of real toy samples (n = 18), which confirmed its appealing potential for toy safety screening and consumer health protection.

The Use of Extraction on C18-Silica-Modified Magnetic Nanoparticles for the Determination of Ciprofloxacin and Ofloxacin in Meat Tissues.

A simple, fast, and low-cost method of extraction using magnetic nanoparticles was developed for sample preparation in the determination of ciprofloxacin and ofloxacin in meat tissues with the use of capillary electrophoresis. This study is the first utilization of silica-coated magnetic nanoparticles with attached C18 chains to extract fluoroquinolones from meat tissues. This method is therefore characterized by a very simple sample preparation procedure, but on the other hand, by satisfactory precision and accuracy. Magnetic nanoparticles with an appropriately modified surface were placed in an Eppendorf tube, then conditioned with methanol, next rinsed with water and, finally, a homogenized tissue sample was added. At the neutral pH of the sample solution, these compounds do not have a charge and are able to adsorb on the modified particles. After extraction, the nanoparticles were dried and, then, desorption of analytes was conducted with the use of a mixture of 0.1 mol/L HCl and acetonitrile (1:1). This approach made it possible to purify the sample matrix and to obtain satisfactory LOQ levels for the method using the CE technique with UV-Vis detection. In this method, the LOD and LOQ values for both analytes were 0.04 nmol/g tissue and 0.15 nmol/g tissue, respectively. The calibration curves were linear in the entire concentration range, and the accuracy and the recovery of the method were at the satisfactory levels. The square value of the linear correlation coefficients (R2) for Cpx and Ofx were 0.9995 and 0.9992, respectively. The precision value of the method was within the range of 3-11% and accuracy was in the range of 93-110%.

Introduction of a totally green and one-step analytical method resulting in exhaustive extraction of some fungicides and herbicides from apple juice using one-pot-synthesized CoGA MOF through dispersive micro solid phase extraction coupled to HPLC-DAD

The first-ever attempt at the use of CoGA metal–organic framework (MOF) in an analytical method development was done in this study to extract and preconcentrate some fungicides and herbicides from apple juice samples. Application of no organic solvents, gallic acid as the ligand, cobalt as the cationic center, no high temperatures or long reaction times in the synthesis process, and one-pot synthesis feature all benefit the introduced method. Also, conducting a one-step extraction process (dispersive micro solid phase extraction) using µL level of the mobile phase as the desorption solvent, and a low amount of MOF utilization make the approach green. The developed analytical process was propelled by dispersing only 15 mg of CoGA MOF in Na2SO4 dissolved aqueous solution containing the analytes and vortexing. After the centrifugation, a µL level of the mobile phase was used for analyte desorption aim and an aliquot of it was injected into the high performance liquid chromatography coupled to a diode array detector. Reaching the exhaustive extraction conditions (extraction recoveries in the range of 95–99%) promises no analyte loss during the extraction process. Also, low limits of detection (0.063–0.220 µg/L) and quantification (0.210–0.740 µg/L), enrichment factors in the range of 47.5–49.5, and low relative standard deviations (2.57–3.70%) for intra-day (n = 5) precisions were achieved. Also, the diode array detector capability helped to distinguish the appeared peaks at the retention times of the analytes in the surveyed juice samples.

Innovative thermally assisted on-line solid phase extraction-reversed phase liquid chromatography applied to targeted nutrimetabolomics in human biofluids

In this study, the use of thermal desorption in on-line solid phase extraction coupled with reversed phase liquid chromatography (on-line SPE-LC) was for the first time proposed and demonstrated for the desorption of analytes strongly retained by multiple interaction polymeric sorbents. In detail, this analytical strategy was applied to the on-line SPE-LC targeted analysis of a model set of 34 human gut metabolites characterized by heterogeneous physicochemical properties (i.e., octanol–water partition coefficient in the range −0.3 – 3.4). The novel thermally assisted on-line SPE approach was investigated in comparison to conventional room temperature desorption strategies based on the use of (i) an optimized elution gradient or (ii) organic desorption followed by post-cartridge dilution. The thermally assisted desorption strategy has been shown to be better performing and suitable for the development of a reliable and sensitive method for the analysis of the model group of analytes in urine and serum. In more detail, under the optimized experimental conditions, the proposed method provided negligible matrix effects in both biofluids for almost all target analytes. Moreover, method quantification limits were in the ranges 0.026–7.2 μg L−1 and 0.033–23 μg L−1 for urine and serum, respectively, i.e., comparable to or lower than those reported in methods previously published.

Sustainable and Rapid Determination of Two Halogenated Pesticides in a Commercial Formulation by Solid Phase Microextraction and Liquid Phase Chemical Ionization Mass Spectrometry

This work presents a sustainable and rapid method for halogenated pesticide analysis without chromatographic separation. The system is composed of a microfluidic open interface (MOI) for solid-phase microextraction (SPME) liquid phase desorption, connected to a liquid electron ionization mass spectrometry interface (LEI-MS). Either a triple quadrupole mass spectrometer (QQQ-MS/MS, (low-resolution) or a quadrupole-time-of-flight tandem MS (QTOF-MS/MS, high-resolution) were employed, each operating in negative chemical ionization (NCI) conditions. The flow rate used (100 µL/min) to rapidly empty the MOI chamber (approximately 2.5 µL) is reduced to the working flow rate of the LEI interface (500 nL/min) by a passive flow splitter (PFS). NCI is an appropriate ionization technique for electrophilic compounds, increasing specificity and reducing background noise. Two halogenated pesticides, dicamba and tefluthrin, were extracted simultaneously from a commercial formulation matrix (CF) using a C18 fiber by direct immersion (3 min under vortex agitation). Analyte desorption occurred in static conditions inside MOI filled with acidified acetonitrile (ACN) (0.2% phosphoric acid, PA). Extraction and desorption steps were optimized to increase efficiency and accelerate the process. No chromatographic separation was involved; therefore, the system fully exploited MS/MS selectivity and HRMS accuracy demonstrating good linearity, repeatability and limits of detection (LODs) and limits of quantification (LOQs) in the pg/mL range (50 and 500 pg/mL, respectively). Low-resolution experiments showed that matrix effects (ME) did not affect the results. The fast workflow (5 min) makes the system suitable for high-throughput analysis observing the principles of green analytical chemistry (GAC).

Microflow liquid chromatography – multi-emitter nanoelectrospray mass spectrometry of oligonucleotides

While the most sensitive LC-MS methods for oligonucleotide analysis contain ion-pairs in the mobile phase, these modifiers have been associated with instrument contamination and ion suppression. Typically, entire LC-MS systems are reserved for oligonucleotide LC-MS when using ion-pairing buffers. To overcome these limitations, numerous HILIC methods, liberated from ion-pairs, have been recently developed. Since ion-pairs play a role in analyte desorption from ESI droplets, their removal from mobile phases tend to impact method sensitivity. An effective way to recover MS sensitivity is to reduce the LC flow rate and therefore reduce ESI droplet size. With a focus on MS sensitivity, this study investigates the applicability of a microflow LC- nanoelectrospray MS platform in oligonucleotide ion-pair RP and HILIC LC-MS methods. The platform is effective and substantially increased the MS sensitivity of HILIC methods. Furthermore, LC method development for both types of separations provide insight into microflow chromatography of oligonucleotides, an under investigated chromatographic scale.

Application of oxidized multi-walled carbon nanotubes and zeolite nanoparticles for simultaneous preconcentration of codeine and tramadol in saliva prior to HPLC determination

In this work, a dispersive micro-solid phase extraction technique along with high-performance liquid chromatography-UV detection was developed for simultaneous preconcentraion and determination of trace levels of codeine and tramadol in human saliva. This method is based on the adsorption of codeine and tramadol on a mixture of oxidized multi-walled carbon nanotubes and zeolite Y nanoparticles with 1:1 ratio as an efficient nanosorbent. Various analytical parameters influencing the adsorption step including the amount of adsorbent, the pH of the sample solution, the temperature, the stirring rate, the contact time of the sample solution, and the adsorption capacity were investigated. Based on the results, 10 mg adsorbent, sample solutions with pH = 7.6, temperature of 25 °C, stirring rate 750 rpm and contact time 15 min, in the adsorption step shows the best results for both drugs. Then the effective parameters on the analyte desorption stage such as the type of desorption solution, pH of the desorption solution, desorption time and desorption volume were investigated. Studies have shown that water/methanol (50:50 v/v) as desorption solution, pH = 2.0, desorption time of 5 min and desorption volume of 2 ml gives the best results.Chromatographic separation was performed on a RP-Shim-pack CLC-ODS-C18 column (250 mm × 4.6 mm, 5 µm) with isocratic mode. The mobile phase contained of acetonitrile:phosphate buffer (18:82, v/v) at pH = 4.5 and the flow rate was 1 ml.min−1. The wavelength of UV detector was adjusted at 210 nm and 198 nm for codeine and tramadol, respectively.Under optimum conditions, the extraction efficiencies of 98.5% and 99.2% were achieved for codeine and tramadol respectively. Enrichment factor of 13, detection limit of 0.3 μg L−1, relative standard deviation of 4.07 for codeine; and an enrichment factor of 15, a detection limit of 0.15 μg L−1, and standard deviation of 2.06 for tramadol were calculated. The linear range of the procedure for each drug was 1.0 to 1000 μg L−1. This method was successfully applied for the analysis of codeine and tramadol in saliva samples.

Development of the thin film solid phase microextraction (TF-SPME) method for metabolomics profiling of steroidal hormones from urine samples using LC-QTOF/MS.

In the present study, the development and optimization of a thin film solid phase microextraction method (TF-SPME) was conducted for metabolomics profiling of eight steroid compounds (androsterone, dihydrotestosterone, dihydroepiandrosterone, estradiol, hydroxyprogesterone, pregnenolone, progesterone and testosterone) from urine samples. For optimization of extraction method, two extraction sorbents (PAN-C18 and PS-DVB) were used as they are known to be effective for isolation of low-polarity analytes. The stages of sample extraction and analyte desorption were considered as the most crucial steps in the process. Regarding the selection of the most suitable desorption solution, six different mixtures were analyzed. As a result, the mixture of ACN: MeOH (1:1, v/v) was chosen in terms of the highest analytes' abundances that were achieved using the chosen solvent. Besides other factors were examined such as the volume of desorption solvent and the time of both extraction and desorption processes. The analytical determination was carried out using the ultra-high performance liquid chromatography coupled with high resolution tandem mass spectrometry detection in electrospray ionization and positive polarity in a scan mode (UHPLC-ESI-QTOF/MS). The developed and optimized TF-SPME method was validated in terms of such parameters as extraction efficiency, recovery as well as matrix effect. As a result, the extraction efficiency and recovery were in a range from 79.3% to 99.2% and from 88.8% to 111.8%, respectively. Matrix effect, calculated as coefficient of variation was less than 15% and was in a range from 1.4% to 11.1%. The values of both validation parameters (recovery and matrix effect) were acceptable in terms of EMA criteria. The proposed TF-SPME method was used successfully for isolation of steroids hormones from pooled urine samples before and after enzymatic hydrolysis of analytes.

Online coupling of matrix solid-phase dispersion to direct analysis in real time mass spectrometry for high-throughput analysis of regulated chemicals in consumer products

The current work is the first study on online coupling of matrix solid-phase dispersion (MSPD) to direct analysis in real time mass spectrometry (DART-MS) bridging with solid-phase analytical derivatization (SPAD) based on a graphene oxide nanosheets (GONs)-coated cotton swab. Proof-of-concept demonstrations were explored for high-throughput analysis of a diversity of regulated chemicals in consumer products such as textiles, toys, and cosmetics. On-demand sorbent combinations were blended with samples, packed into MSPD columns, and mounted on a homemade 3D-printed rack module for automated sample feeding. To achieve good synergy between MSPD and DART-MS, a cotton swab with a conical tip deposited with GONs was attached to the bottom of the MSPD column. The swabs serve as a solid-phase microextraction probe for convenient enrichment of the eluted analytes from MSPD, thermal desorption of the enriched analytes by DART, and sensitive detection by a hybrid quadrupole-Orbitrap mass spectrometer. Furthermore, the utility of an on-swab SPAD strategy was demonstrated for the detection of formaldehyde by use of the derivatizing reagent of dansyl hydrazine, contributing to improved ionization efficiency without compromising the overall coherence of the analytical workflow. The MSPD-DART-MS methodology was systematically optimized and validated, obtaining acceptable recovery (71.7–110.3%), repeatability (11.8–19.3%), and sensitivity (limits of detection and quantitation in the ranges of 6.2–19.5 and 23.7–75.9 μg/kg) for 32 target analytes. The developed protocol streamlined sample extraction, clean-up, desorption, ionization, and detection, highlighting the appealing potential for high-throughput analysis of samples with complex matrices.