Analysis Of Receptor-mediated Endocytosis Research Articles

Asialoglycoprotein Receptor

This chapter discusses the intracellular traffic of the asialoglycoprotein (ASGP) receptor and its ligands and integrates this information into the current understanding of intracellular compartments. Complementary DNA (cDNA) cloning and sequence analysis have provided insights into the receptor structure, and specific signals for the intracellular sorting are beginning to be identified. Since its discovery, the ASGP receptor has provided a useful experimental model for a variety of important biological processes. It has been instrumental in the analysis of insertion of proteins into the endoplasmic reticulum (ER) membrane, protein degradation in the ER, transport to the basolateral plasma membrane domain in epithelial cells, and, most prominently, in the analysis of receptor-mediated endocytosis. The receptor is a classical marker for the early endocytic compartments, while its ligands serve to delineate the intracellular organelles along the degradative route from the plasma membrane to lysosomes. The availability of the cDNAs of several endocytic receptors, among which is the ASGP receptor, is the basis of recent progress in the identification of sorting signals.

The analysis of receptor-mediated endocytosis by centrifugation in isoosmotic gradients.

AbstractReceptor-mediated endocytosis is an important mechanism by which the cell is able to take in macromolecules from the external environment and carry them through the necessary metabolic processes. Studies of the sequential steps of these events require methods of following the fate of the molecule from its internalization to its degradation in lysosomes or its transfer to other intracellular compartments.KeywordsIncubation BufferBuoyant DensityShort Incubation PeriodLysosomal ActivitySoluble ActivityThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.