Analysis Of Naproxen Research Articles

High performance liquid chromatography with photo diode array for separation and analysis of naproxen and esomeprazole in presence of their chiral impurities: Enantiomeric purity determination in tablets

A stereoselective high performance liquid chromatographic method with diode array detection (HPLC-DAD) was introduced for S-naproxen and esomeprazole determination in tablets. The separation was achieved on a Kromasil Cellucoat chiral column using a mobile phase consisting of hexane: isopropanol: trifluoroacetic acid (TFA) (90:9.9:0.1 v/v/v). The proposed system was found to be suitable for the enantioseparation of naproxen and omeprazole biologically active isomers. After optimization of the chromatographic conditions, resolution values of 3.84 and 2.17 could be obtained for naproxen and omeprazole isomers, respectively. The method was fully validated for the determination of S-isomers of each drug in their dosage form. Also, the enentiomeric purity was determined in commercial tablet containing S-naproxen and esomeprazole. The enantiomeric purity was calculated for each drug and the chiral impurities (R-isomers) could be determined at 1% level. The method was validated and good results with respect to linearity, precision, accuracy, selectivity and robustness were obtained. The limits of detection (LOD) and quantification (LOQ) were 2.00, 6.50 and 0.10, 0.35μgmL−1 for S-naproxen and esomeprazole, respectively.

Development and Validation of Liquid Chromatography- Mass Spectroscopy/Mass Spectroscopy Method for Quantitative Analysis of Naproxen in Human Plasma after Liquid-Liquid Extraction

Purpose: To determine naproxen levels in human plasma using a new liquid chromatography-Mass spectroscopy/Mass spectroscopy (LC-MS/MS) method that involves a simple and single step extraction procedure using low-cost reagents. Method: A novel liquid chromatography‐tandem mass spectrometry method for the quantitative determination of naproxen in human K2-EDTA plasma in negative ion mode was employed and validated using zidovudine as internal standard (IS). Sample preparation was accomplished by liquidliquid extraction technique. The eluted samples were chromatographed on Zorbax Eclipse XDB phenyl 4.6 ◊ 75 mm, 3.5 µm column (Agilent Technologies) using a mobile phase consisting of acetonitrile: 20 mM ammonium acetate (90:10 v/v).The injection volume was 15 µL and the total run time was 3.0 min. The method was validated for all parameters for naproxen. Results: The method showed selectivity and linearity over a concentration range of 500.1 ng/mL to 100028.5 ng/mL The validation data indicate precision and accuracy of 90 - 110 % and < 15 %), respectively, as well as recovery (80.63 %), stability (mostly stable) and carryover (0 %). Conclusion: A rapid and selective LC-MS/MS method for the quantification of naproxen in human plasma has been developed and can be used in therapeutic drug monitoring of this drug as well as in bioequivalence studies of the drug.

Fluorescent polyacrylamide nanoparticles for naproxen recognition

We present the synthesis of fluorescent acrylamide nanoparticles (FANs) capable of recognizing non-steroidal anti-inflammatory drugs (NSAIDs) in buffered aqueous solutions. Within this important group, we selected naproxen, one of the 2-arylpropionic acids (profens), due to its use for the treatment of moderate pain, fever, and inflammation. The nanosensors were prepared under mild conditions of inverse microemulsion polymerization using aqueous acrylamide as the monomer and N,N'-methylenebisacrylamide as the cross-linker, employing the surfactants polyoxyethylene-4-lauryl ether (Brij 30) and sodium bis(2-ethylhexyl)sulfosuccinate in hexane. Furthermore, a fluorescent monomer, (E)-4-[4-(dimethylamino)styryl]-1-[4-(methacryloyloxymethyl)benzyl]pyridinium chloride (mDMASP) has been synthesized and incorporated into the nanoparticles. The nanosensors exhibit a broad absorbance at around 460 nm and a structureless fluorescence band with maximum at 590 nm in 0.5 M phosphate buffer (pH = 7.2). The recognition process is performed on the basis of ionic interactions which are monitored by the fluorescence increase at 590 nm upon addition of different concentrations of naproxen. The FANs show a size distribution in the range of 20-80 nm, with a hydrodynamic diameter of 34 nm. In order to assess the selectivity of the FANs, a systematic study was conducted on the effect produced by drugs and biomolecules that could interfere with the analysis of naproxen.

Determination of Naproxen and its Metabolite, 6‐O‐Desmethylnaproxen, in Human Urine by Capillary Isotachophoresis

Analysis of naproxen (NP) and 6‐O‐desmethylnaproxen (DNP) in human urine samples was carried out using a column‐coupling isotachophoretic analyzer equipped with a conductivity detector. The preseparation capillary (80 mm×0.8 mm I.D.) was connected with an analytical capillary (160 mm×0.3 mm I.D.). The preseparation capillary was filled with the leading electrolyte (LE): 20 mM hydrochloric acid adjusted with creatinine to pH 5.0; 0.1% methylhydroxypropylcellulose. The analytical capillary was filled with the LE: 10 mM hydrochloric acid adjusted with β‐alanine to pH 4.0; 0.1% methylhydroxypropylcellulose. The terminating electrolyte was 10 mM 2‐(N‐morpholino)‐ethanesulfonic acid adjusted with tris(hydroxymethyl) aminomethane to pH 6.9. Limit of quantitation was 1.4 µg/mL for NP and 0.5 µg/mL for DNP. The proposed method was successfully applied to the direct determination of free NP and DNP in urine samples. The total of free and conjugated NP and DNP was obtained by including an alkaline hydrolysis step.

Preliminary study on the synthesis and high‒resolution NMR analysis of Naproxen and Ibuprofen esters

The synthesis and NMR analysis of several diastereoisomeric Naproxyl–Naproxenate, Naproxyl–Ibuprofenate and Ibuprofyl–Naproxenate esters are reported. The NMR high resolution spectra run in presence of the chiral shift reagents and variable temperature analysis were done to study the dynamic properties of these esters.

Application of fluorimetry to the analysis of Naproxen and its complexation with modified β-cyclodextrins

Application of fluorimetry to the analysis of Naproxen and its complexation with modified β-cyclodextrins

A High-Performance Liquid Chromatographic Method for the Quantitative Determination of Naproxen and Des-Methyl-Naproxen in Biological Samples

Abstract A HPLC method for the quantitative analysis of naproxen and its major metabolite des-methyl-naproxen in biological fluid samples is described. Two methods of detection are compared: U.V. spectrophometry and spectrophotofluorometry. In both procedures an internal standard is used: diflunisal in the U.V. procedure and the ethoxy-analog of naproxen during fluorometry. The sensitivity of the fluorometric detection is higher than that of the U.V. detection; the limit being respectively 0.1 μg and 2.0 μg per milliliter sample. The fluorescence detection procedure can also be applied to very small samples (0.05 ml) in the therapeutic concentration rang range. Both procedures have been applied to clinical and laboratory studies in which they appear to be very satisfactory because of their ease of handling and their suitability for routinely performed analysis.