Analysis Of Morphological Diversity Research Articles

Diversity and distribution of Sargassum spp on Lombok Island

Sargassum is a genus that grows naturally in waters with strong currents and rocky bottoms. This genus has high diversity and distribution, including on Lombok Island. This research aims to analyze the diversity and distribution of sargassum on Lombok Island. Research sampling was carried out in 15 locations spread across 4 districts on Lombok Island. Morphological diversity analysis was carried out using MVSP 3.1 software. Meanwhile, distribution is carried out by sampling the distribution in the waters of Lombok Island. The research results showed that the number of Sargassum species found was 4 species. Each species found from different sampling locations has variations with a similarity percentage of 52.8% to 100%. Sargassum was found in 15 sampling locations with the following details: Sargassum polycystum was found in five locations, Sargassum crassifolium in 5 locations, Sargassum cristaefolium in 7 locations, and Sargassum sp in 3 locations.

The Analysis of Morphological Diversity and Polyphenols Content of Celosia cristata in M2 Population Induced by Ethyl Methane Sulphonate

Celosia cristata, an edible ornamental plant, is a potential floricultural commodity that needs further improvement to increase its agro-morphological characters and polyphenol content. Induced mutagenesis using ethyl methane sulphonate (EMS) is an effective tool to increase genetic diversity that has been applied in many plant species. This study aimed to assess the morphological diversity, polyphenol content, and antioxidant activities of C. cristata mutagenized by EMS in the M2 generation. A total of 230 M2 plants generated from the M1 generation were evaluated in this study and the polyphenols content and antioxidant activities analysis were conducted on fifteen selected M2 plants. Polyphenols content was analyzed using the Folin-Ciocalteu method and colorimetric method with slight modification, and the antioxidant activities investigated using 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay with minor changes. There are six subpopulations with the highest diversity of quantitative characters in the M2 population in quantitative characters, i.e. C2-17-1.0%, C2-1-0.7%, C2-20-2.0%, C2-25-0.7%, C2-1-0.9%, and C2-1-0.7%. Changes in the shape and color of leaves, stems, and flowers of C. cristata were also observed in the M2 population. C2.1, C2.6, and C2.12. 2.1, C2.6, and C2.12 are potential plants derived from EMS mutagenesis with the highest polyphenol content and antioxidant capacity in the M2 population. In conclusion, induced mutation using EMS can enhance the agro-morphological diversity, polyphenols content along with the antioxidant activities of C. cristata, and demonstrate the successful mutation breeding program.

Characterization of diverse sesame (Sesamum indicum L.) panel through morphological and molecular marker analysis

Sesame is one of the most important and ancient oilseeds crop with high and good quality oils content. Present Investigation was carried out for the assessment of the genetic diversity using 33 sesame genotypes through morphologically and 21 SSR markers. In the morphological diversity analysis, nine clusters were observed with 33 genotypes using all phenological, yield and its contributing attributes. Among the 9 clusters, cluster II had maximum number of genotypes (14) followed by cluster III (6), cluster IV (5), cluster I (3), cluster V, VI, VII, VIII and IX had one genotype to each cluster. The highest inter-cluster distance was observed between cluster VI and I (386.70), which indicates maximum diversity between the genotypes of these clusters, could be further used in crossing programme. However, SSR markers grouped 33 genotypes into six clusters at 0.51 dissimilarity index. In which cluster V had maximum (7) genotypes and cluster VI had minimum (3) genotypes. The results of genetic diversity tallied with the morphological diversity, such as BRT-04 are the farthest from BRT-08, BRT-09 and BRT-10, however BRT-08, BRT-09 and BRT-10 are in the same cluster in both morphological and molecular diversity analysis. The UPGMA dendrogramwas constructed using Jaccard's dissimilarity coefficients based on SSR markers score on thirty-three genotypes. Polymorphism information content (PIC) value varied between 0.15 and 0.36 with an average of 0.27. The genotypes from the distinct clusters can be utilized for hybridization programme to recover heterotic pools.

GENETIC DIVERGENCE AMONG MAIZE (ZEA MAYS L.) INBRED LINES USING MORPHOMETRIC MARKERS

Genetic divergence study is imperative in breeding programmes in order to select distant parents. Forty maize inbred lines were assessed using morphometric markers to identify the potential inbred lines for yield and its component traits. The inbred lines were raised in α-RBD design replicated twice during kharif 2019. The morphological diversity analysis was done as per the standard statistical procedures using Mahalanobis D2-statistic. Analysis of variance revealed significant mean sum of squares due to genotypes for all the traits suggesting ample amount of genetic variability present among them. Moderate PCV, GCV and high heritability coupled with moderate genetic advance was observed for grain yield per plant, plant and cob placement height. Grain yield per plant exhibited significant positive correlation with all the traits except days to 50 per cent pollen shed and silking. Path analysis revealed high direct effects of days to 50 per cent pollen shed, harvest index, grains per row and 100 grain-weight on grain yield per plant therefore these morphometric traits would be the best selection indices to select high yielding genotypes. D2-statistics grouped 40 maize genotypes into three clusters. Cluster III has the highest cluster mean values for all the traits.Seven genotypes viz., 4186-4-05-1-1, LM-14, BAJIM-08-27, BIOM 10, LQPM24×1114-5, 9180-2, LDH 5(Vivek-21-1) were superior over the best check on the basis of mean performance and disease resistance and were diverse therefore, could be used in maize improvement program.

Genetic and morphological diversity analysis of lime and acidic Citrus spp. from two germplasm collections in Thailand

Genetic and morphological diversity analysis of lime and acidic Citrus spp. from two germplasm collections in Thailand

Characterization of morphological units in a small, forested stream using close-range remotely piloted aircraft imagery

Abstract. Forested, gravel-bed streams possess complex channel morphologies which are difficult to objectively characterize. The spatial scale necessary to adequately capture variability in these streams is often unclear, as channels are governed by irregularly spaced features and episodic processes. This issue is compounded by the high cost and time-consuming nature of field surveys in these complex fluvial environments. In larger streams, remotely piloted aircraft (RPA) have proven to be effective tools for characterizing channels at high resolutions over large spatial extents, but to date their use in small, forested streams with closed forest canopies has been limited. This paper seeks to demonstrate an effective method for classifying channel morphological units in small, forested streams and for providing information on the spatial scale necessary to capture the dominant spatial morphological variability of these channels. This goal was achieved using easily extractable data from close-range RPA imagery collected under the forest canopy (flying height of 5–15 m above ground level; ma.g.l.) in a small (width of 10–15 m) stream along its 3 km of salmon-bearing channel. First, the accuracy and coverage of RPA for extracting channel data were investigated through a subcanopy survey. From these survey data, relevant cross-sectional variables (hydraulic radius, sediment texture, and channel slope) were extracted from high-resolution point clouds and digital elevation models (DEMs) of the channel and used to characterize channel unit morphology using a principal component analysis-clustering (PCA-clustering) technique. Finally, the length scale required to capture dominant morphological variability was investigated from an analysis of morphological diversity along the channel. The results demonstrate that subcanopy RPA surveys provide a viable alternative to traditional ground-based survey approaches for mapping morphological units, with 87 % coverage of the main channel stream bed achieved. The PCA-clustering analysis provided a comparatively objective means of classifying channel unit morphology with a correct classification rate of 85 %. An analysis of the morphological diversity along the surveyed channel indicates that reaches of at least 15 bankfull width equivalents are required to capture the channel's dominant morphological heterogeneity. Altogether, the results provide a precedent for using RPA to characterize the morphology and diversity of forested streams under dense canopies.

Evaluation of genetic diversity of parental lines for development of heterotic groups in hybrid rice (Oryza sativa L.)

Knowledge of genetic diversity and potential heterotic relationships among parental lines is of significant importance in hybrid rice breeding programs. In the present study, in order to understand the genetic diversity among 96 parental lines, they were characterized for their diversity with respect to their morphological traits (n = 12) and molecular markers using a set of 50 SSR markers. Morphological diversity was estimated using Mahalanobis D2 statistics in terms of generalized group distance. Based on morphological diversity analysis, the 96 lines were grouped into 5 major and 13 monogenotypic clusters. In molecular marker analysis, the parental lines were consistently clustered into B (Maintainer group) and R (Restorer group) groups based on distance and model based approaches. Strong correspondence was observed between the pedigree of parental lines with molecular genotyping based grouping than morphological trait based grouping. From the results of the present investigation, it is evident that the available diversity among the two groups i.e., maintainer group (B) and restorer group (R) is sufficient for developing heterotic hybrids, but within the maintainer and restorer groups, the diversity is limited, the diversity among restorers was moderate, while it was low among the maintainers and hence efforts are needed for broadening their genetic base of parental lines for development and adoption of high-yielding hybrids.

Macroscopy and Microscopy Study of Scopraia dulcis L.

A morphological diversity analysis of Scoparia dulcis by macroscopy and microscopy has been conducted. The aim of this research is to determine the morphological and anatomical character of S. dulcis which grow in Opak watershed, Special Region of Yogyakarta. Samples were taken from Sleman (Cangkringan, Ngemplak Kalasan, and Prambanan) and Bantul (Imogiri and Pundong) region. Selection of sampling location is based on altitude difference. The samples are the third leaf order from the tip of the stem. They were measured in length and width. Microscopy analysis was performed by observing the number of glandular trichomes, palisade cells in the epidermis and stomata in the upper and lower epidermis. The data obtained were analyzed using principal component analysis (PCA). The results show that based on morphological and anatomical assessment, there were found 3 clusters of S. dulcis, i.e. Cangkringan-Ngemplak-Prambanan, Kalasan, and Imogiri-Pundong cluster.

Landscape texture in anthropogenically transformed regions: The example of Upper Silesia and the Dąbrowa Coal Basin (southern Poland)

In recent years, we see growing importance of research on landscape texture, which enables scientists to assess landscape as to its esthetic (visual), planning, as well as ecological aspects. Analyses of landscape texture result in identification of landscape zones, classified according to their habitability, recreational potential and suitability for industry, which plays a crucial role for work on planning and strategic documents. The study area covers 12 selected municipalities of Upper Silesia and the Dąbrowa Coal Basin, which are highly industrialized regions. Combining an analysis of the degree of landscape enclosure/openness with an analysis of morphological diversity in the study area, the author identifies landscape texture units in accordance with the new, more detailed typology. This results in the emergence of 36 landscape texture types that take into account the land relief forms in the study area. For the needs of further analyses, these types are classified into three groups: open, mosaic and enclosed landscapes.

Nutritional compound analysis and morphological characterization of spider plant (Cleome gynandra) - an African indigenous leafy vegetable

Spider plant is among the important indigenous African leafy vegetables having the potential to contribute to food and nutritional security in sub-Saharan Africa. The main objective of this study was to quantify the mineral concentration, to identify and quantify glucosinolates and flavonoids in spider plant and further to characterize spider plant entries using important morphological traits. Thirty spider plant entries from different African countries, comprising of farmers' cultivars, gene bank accessions and advanced lines were grown in a field experiment and harvested for leaves, stems, flowers and siliques at different developmental stages. Five plant types based on the stem and petiole colorations were identified. Significant genotypic differences were shown for all the morphological traits except for 100 seed weight and silique weight. High mineral concentrations in the leaf tissue were observed especially for potassium, calcium, magnesium, phosphorus, iron, manganese and zinc. The aliphatic 3-hydroxypropyl glucosinolate was the main glucosinolate detected in all tissues with the highest concentrations in the reproductive organs. Glycosides of quercetin, kaempferol and isorhamnetin were the main flavonoids. Isorhamnetin glycosides were detected in trace amounts in both, leaves and inflorescences, while quercetin and kaempferol glycosides were the dominant flavonoids in the leaves and inflorescences, respectively. This knowledge of beneficial nutrient contents is an incentive for promoting spider plant consumption for improved human health while the morphological diversity analysis will be important for the further development of the spider plant germplasm.

English

English

Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation

Daucus pusillus Michx. and Daucus montevidensis Link ex Spreng. are wild carrots from the Americas with unresolved taxonomic status. An investigation was carried out with accessions of D. pusillus and (or) D. montevidensis from Argentina for (i) morphological and molecular (amplified fragments length polymorphism (AFLP) and inter simple sequence repeat (ISSR)) characterization, (ii) analysis of congruence of morphological and molecular variation, and (iii) comparison of diversity for the internal transcribed spacer (ITS) region with that reported for a North American accession of D. pusillus. Twelve accessions of D. pusillus and (or) D. montevidensis — representing their geographical distribution in Argentina — and one accession each of wild Daucus carota L. and Daucus montanus Humb. & Bonpl. ex Schult. — as outgroups — were included. In the multivariate analysis of morphological diversity, two accessions were clearly differentiated; this result is not sustained by multivariate analysis of molecular diversity. Based on multivariate and molecular variance (AMOVA) analyses, D. pusillus and (or) D. montevidensis accessions were separated at the molecular level into two groups, associated with geographical origin. Because this result is not supported by morphology, the segregation into two taxa seems unjustified. In all accessions, ITS and 5.8S rDNA regions had identical sequences, which differ in one nucleotide from the corresponding sequence of the North American accession. According to the combined results, D. pusillus would be a single taxon distributed from North to South America, and D. montevidensis a nomenclatural synonym. Autogamy of D. pusillus and its highly structured genetic diversity (Fst = 0.86) allows the application of a geographically targeted approach for germplasm exploration, conservation and eventual use in pre-breeding.

A new species of Cardioglossa (Amphibia: Anura: Arthroleptidae) endemic to Mount Manengouba in the Republic of Cameroon, with an analysis of morphological diversity in the genus

A new frog species of the genus Cardioglossa, that is probably restricted to less than 10 km2 of gallery forest near the summit of Mount Manengouba in the Republic of Cameroon, is described. Unlike all other Cardioglossa, in life the coloration of the new species is typically brownish red, with no dorsal markings, but with a pair of thin, golden white lines that extend posteriorly from the tip of the rostrum and terminate just above and behind the tympanum. This new species is morphologically similar to C. oreas, another high-altitude species endemic to the Cameroonian mountains: both have small tympana and lack the hypertrophied third manual digit typical of males of other Cardioglossa. The tadpole of the new species is similar to that of C. occidentalis, the only other Cardioglossa for which the tadpole is fully described, but differs in having a broad anterolaterally projecting supraoral labium, lacking ventral pigmentation, and having a relatively longer spiracular tube. Several features of tadpole morphology are convergent with the unusual fossorial tadpole of the microhylid Otophryne and suggest a similar convergence in ecology. Principal components analysis of morphometric data, followed by discriminant function analysis, was used to explore patterns of morphological similarity within Cardioglossa. These analyses provide support for five species groups: (1) C. aureoli (distinct from other Cardioglossa); (2) C. escalerae, C. gratiosa and C. nigromaculata; (3) C. gracilis, C. melanogaster and C. schioetzi; (4) C. sp. nov. and C. oreas; and (5) C. cyaneospila, C. pulchra, C. venusta and C. trifasciata. The inclusion of C. cyaneospila in the last group supports previous suggestions of past biotic connections between the Albertine Rift mountains and those of the Cameroon Volcanic Line. The description of this new species further emphasizes Mt Manengouba as a centre of anuran endemism within the Cameroonian mountains and reaffirms the importance of this ecoregion within sub-Saharan Africa. © 2008 The Linnean Society of London, Zoological Journal of the Linnean Society, 2008, 154, 611–630.