Analysis Of Fluconazole Research Articles

Development and Validation of UV Spectrophotometric Method for the Estimation of Fluconazole in the Marketed Dosage Formulations

The aim of current study was to develop and validate UV Spectrophotometric method for Fluconazole. Ultra Violet Spectroscopy was carried out at 260nm and samples were prepared with a solution of phosphate buffer pH 7.4. The linearity demonstrated a correlation coefficient of 0.998. The method was subjected to validity for the parameters as per ICH guidelines. Correctness, exactness, LOD, LOQ, recovery study and range were determined and the parameters were determined by performing the repeated experiments and proper sampling of the solution. The objective of selection of UV spectroscopy was the simplicity, time and economy of the method furthermore the sensitivity was also high by UV spectroscopy as HPLC technique is costly, time consuming and number of factors may affect the determination. It was observed that, the proposed method was linear, correct, repeatable, error free, selective, specific and cost effective proving the dependability of the method. More over same solvent was used throughout the experimental work and it was found that the method was free from any type of interference from any excipients and method was simple, rapid, precise, accurate and sensitive and can be applied in routine analysis of Fluconazole in single and combined form.

Analysis of Fluconazole in Human Urine Sample by High Performance Liquid Chromatography Method

A method for determination of fluconazole, antifungal drug in human urine by using reversed-phased high performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector was developed. Optimization HPLC conditions were carried out by changing the flow rate and composition of mobile phase. The optimum separation conditions at a flow rate 0.85 mL/min with a composition of mobile phase containing methanol:water (70:30, v/v) with UV detection at a wavelength 254 nm was able to analyze fluconazole within 3 min. The excellent linearity was obtained in the range of concentration 1 to 10 μg/mL with r2 = 0.998. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.39 μg/mL and 1.28 μg/mL, respectively. Solid phase extraction (SPE) method using octadecylsilane (C18) as a sorbent was used to clean-up and pre-concentrated of the urine sample prior to HPLC analysis. The average recoveries of fluconazole in spiked urine sample was 72.4% with RSD of 3.21% (n=3).

STABILITY-INDICATING HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF ITS OXIDATIVE DEGRADATION PRODUCT - KINETIC AND STRESS STUDY

A simple, specific, accurate, and stability-indicating high performance liquid chromatographic method (HPLC) method has been established for analysis of fluconazole (FLZ) in the presence of its degradation products generated in the stress degradation study. FLZ was subjected to stress conditions of acid, alkali, and neutral hydrolysis, oxidation, photolysis, and thermal decomposition. Extensive degradation was found to occur in oxidative medium under thermal stress. Successful separation of drug from degradation products was achieved on a C-18 column using phosphoric acid 0.5% v/v: acetonitrile (80:20% v/v) as the mobile phase. The flow rate was 1.5 mL min−1 and the detector was set at 261 nm. The retention times of FLZ and its main oxidative degradation product were found to be 5.389 min and 2.729 min, respectively. Linearity was established for fluconazole in the range of 0.5–50 µg/ml. The percentage recovery of fluconazole was found to be 99.91 ± 0.74. Because the method effectively separates fluconazole from its oxidative degradation products, it can be used as stability-indicating method. The proposed method was also used to study the kinetics of fluconazole oxidative degradation that was found to follow a zero-order reaction. The t1/2 was 21.66 min while k (reaction rate constant) was 2.91 × 10−8 mole/min.

Development and validation of a normal-phase HPTLC-densitometric method for the quantitative analysis of fluconazole in tablets

This paper presents the development and validation of an improved method for the analysis of fluconazole using high-performance thinlayer chromatography (HPTLC) with densitometric detection. Separation was performed on silica gel 60 F254 plates. The mobile phase is comprised of ethyl acetate-methanol-ammonia-diaminoethane (85:10:5:0.5 ν/ν/ν/ν). Detection wavelength was 216 nm and the RF value obtained was 0.4 ± 0.03. An F-test indicated that the calibration graph was linear at the evaluated concentration range. The %RSD for repeatability and intermediate precision were 1.60 and 3.05, respectively. The mean percentage recovery for the trueness was 99.1 ± 2.1%. This method was successfully used to analyze the fluconazole content in marketed tablet samples.