Analysis Of Cosmetic Products Research Articles

Study of pig dna analysis methods in cosmetics: a review

Collagen is one of the components used for cosmetics. Functionally, collagen is derived from pig fat, which functions as a skin conditioning agent (Emollient), emulsion stabilizer (Emulsifier), and as ingredient to increase the viscosity of cosmetic preparations. The method that can detect pig content is the PCR method, which can confirm the presence of DNA content in pigs. In this study, an analysis of cosmetic products containing porcine DNA was carried out using the PCR method. This research was divided into two stages: the initial stage was DNA extraction, and the second stage was PCR analysis. This research is a study through literature study using the narrative review technique. This study aimed to collect information and examine the analysis method of identifying pig DNA contained in cosmetics to obtain the right and accurate way through a literature approach. From the literature study results, it can be concluded that the best extraction method for the isolation of porcine DNA in cosmetic preparations is the extraction method with the Boom Method, while the DNA analysis used to identify porcine DNA in cosmetics is RT-PCR (Real-time Polymerase Chain Reaction). Conventional PCR is because both methods have their respective advantages. The RT-PCR (Real-time Polymerase Chain Reaction) method can amplify and quantify the number of target DNA molecules. The Conventional PCR Method can form DNA bands based on the amplification value. 

A comprehensive review on the use of microextraction techniques in the analysis of cosmetic products

• Contributions in the analysis of cosmetics based on microextraction are described. • Trends within the topic have been properly discussed. • Novel extraction phases and benefits they provide over traditional ones are highlighted. Microextraction techniques have gained special relevance since their appearance in the 90s, and the analysis of cosmetic products has not been immune to this trend. The first application of microextraction techniques in this field was published 25 years ago and, since then, these techniques have become the most widely used methodologies in the analysis of these everyday products, demonstrating that the advantages they provide in sample preparation are in line with the needs and principles currently pursued. This review presents a comprehensive description of those contributions in the analysis of cosmetic products based on microextraction techniques, from the first precedent to the present. The trends within the topic have been properly discussed, highlighting the most recent extraction phases and the benefits they present over the traditional ones.

Chemical and biological properties of anti-wrinkle peptide Argireline

Argireline, a peptide with the sequence: Ac-Glu-Glu-Met-Gln-Arg-Arg-NH2, also known as Acetyl Hexapeptide-8, reduces facial lines and wrinkles by destabilization of the formation of the SNARE complex (SNAP Receptor, soluble N-ethylmaleimide sensitive factor (NSF) attachment protein receptor), thus preventing muscle contraction. It is a biosafe cosmetic alternative to the botulinum toxin. The method of choice in bioactive peptide analysis is reversed-phase high performance liquid chromatography coupled with mass spectrometry (LC-MS). The aim of this work as to present the properties of Argireline and the analysis of cosmetic products containing this peptide. Previous reports on possible Argireline transformations in cosmetic formulations have not confirmed deacetylation, whereas the oxidation of methionine residue was detected by our team. As the biological activity of the oxidized Argireline is not known, further biological studies, as well as efficient analytical procedures for transformation monitoring and quality control in cosmetic products, are necessary.

[Research progress in sample pretreatment and analytical techniques for cosmetics].

With the continuous economic development and improvement of living standards, cosmetic products are being widely used as daily consumer products, and there are increasing concerns about their safety. Consequently, there is a substantial increase in the number of cosmetic samples subjected to various analyses, and this in turn necessitates more stringent requirements for the related analytical techniques. However, traditional sample pretreatment and analytical techniques are time-consuming, require large amounts of organic solvents, and have low throughput, thus failing to meet the current demand for green analytical chemistry. To address this issue, researchers have developed numerous environmentally friendly pretreatment techniques as well as high-throughput and rapid on-site methods to ensure the quality and safety of cosmetics. This paper reviews the current progress in sample pretreatment and analytical techniques for cosmetics and discusses the trends and prospects, which may provide technical guidance for researchers and inspectors in the analysis of cosmetic products.

Size analysis of nanoparticles in commercial O/W sunscreens

Nanoparticles are employed in a variety of applications and especially in cosmetics the issue is discussed whether or not they can be regarded as safe. Analysis of nanosized structures and morphology studies prove to be difficult in many aspects. Nevertheless, there is the demand for new, cost-effective and simple yet reliable methods of analysis to assess the occurrence of nanoparticles in cosmetics in order to evaluate the possible risks conditioned by nanosized structures. In the present study, a simple method was developed to extract particles from commercial sunscreens that are O/W emulsions to measure the particle size of suspended material by laser diffraction. A following, simple calculation based on the specific surface area and particle size distribution allows distinguishing agglomerated nanoparticles from larger particles and thereby contributes well to the tools in analysis of cosmetic products. It was possible to create a simple, fast and cost-effective method to obtain an overview whether nanoparticles are included in a cosmetic product or not.

Gamma irradiation, rheological and microbiological analysis of cosmetic products

In this study, we aimed to use gamma irradiation for decontamination of cosmetic product (gel) achieve the acceptable microbiological limits. Cosmetic product vials were irradiated (10-100 kGy) and physicochemical, microbiological and biological properties of these samples were evaluated in normal conditions. Decontamination dose for all samples was found to be about 10 kGy or below. A pseudoplastic feature was induced in the gel after irradiation suggesting a large applicability of gamma radiation to this purpose.

Comparative analysis of mercury content in human hair and cosmetic products used in Dar es Salaam, Tanzania

Total mercury (T-Hg) concentrations were analysed in human hairs and cosmetic products sold in Dar es Salaam Tanzania. The average total mercury (T-Hg) concentrations in the scalp hair of females using mercury based cosmetic creams and soaps ranged from 7.0 ± 0.4 to 880 ± 12 ppm. Highest T-Hg concentrations were found in females who work in beauty hair salons and the lowest concentrations were found among students. The average values in non-cosmetic users were found to be lower, with mercury ranging from 0.57 ± 0.07 to 8.29 ± 1.22 ppm. Analysis of cosmetic products indicated high values, ranging from 0.11 ± 0.03 to 8665 ± 15 ppm in soaps and 0.16 ± 0.02 to 25.30 ± 4.2 ppm in cosmetic creams. Mercury being a cumulative toxin, the findings suggests that women who use mercurial crèmes and soaps are likely to be poisoned as the compounds can enter the body through various pathways, including inhalation of vapor, ingestion, and skin contact. Tanzania Journal of Science Vol. 31 (1) 2005: pp. 83-90

Analysis of cosmetic products by gas chromatography/mass spectrometry and fast-atom bombardment mass spectrometry: when a combined approach is successful

Fast-atom bombardment mass spectrometry (FAB-MS, both in the positive-and negative-ion modes) and gas chromatography/mass spectrometry (GC/MS) were applied for the rapid identification of the main ingredients of two widely employed cosmetic products, a mascara and a semipermanent hair dye. FAB-MS, on the untreated product (hair dye) or on a crude methanol extract (mascara) gives an unequivocal fingerprint of involatile constituents, i.e. surfactants, anionic (cetyl/stearyl soaps) in mascara and cationic (polyethoxylated oleyl/linoleyl/ricinoleyl-methyl ammonium chloride) in the hair dye. GC/MS (n-hexane and chloroform extracts), detects hydrocarbons, volatile silicon compounds, perfume components, preservatives and antioxidants in mascara and fatty acids (C8–C18), dye solvents and 4 colouring agents: 2-amino-5-nitrophenol, HC Yellow 2 (N-2-hydroxyethyl-o-nitroaniline); O-(2-hydroxyethyl)-4-amino-3-nitrophenol and HC Red3, N-(2-hydroxyethyl)-2-nitro-p-phenylendiamine in the hair dye. These results demonstrate that the combined FAB-MS and GC/MS approaches, enabling a complete picture of the constituents of the cosmetic matrix, provide an invaluable analytical tool for the quality control of cosmetics, a subject that has been rather neglected to date. © 1997 John Wiley & Sons, Ltd.

Analysis of cosmetic products by gas chromatography/mass spectrometry and fast‐atom bombardment mass spectrometry: when a combined approach is successful

Fast-atom bombardment mass spectrometry (FAB-MS, both in the positive-and negative-ion modes) and gas chromatography/mass spectrometry (GC/MS) were applied for the rapid identification of the main ingredients of two widely employed cosmetic products, a mascara and a semipermanent hair dye. FAB-MS, on the untreated product (hair dye) or on a crude methanol extract (mascara) gives an unequivocal fingerprint of involatile constituents, i.e. surfactants, anionic (cetyl/stearyl soaps) in mascara and cationic (polyethoxylated oleyl/linoleyl/ricinoleyl-methyl ammonium chloride) in the hair dye. GC/MS (n-hexane and chloroform extracts), detects hydrocarbons, volatile silicon compounds, perfume components, preservatives and antioxidants in mascara and fatty acids (C8–C18), dye solvents and 4 colouring agents: 2-amino-5-nitrophenol, HC Yellow 2 (N-2-hydroxyethyl-o-nitroaniline); O-(2-hydroxyethyl)-4-amino-3-nitrophenol and HC Red3, N-(2-hydroxyethyl)-2-nitro-p-phenylendiamine in the hair dye. These results demonstrate that the combined FAB-MS and GC/MS approaches, enabling a complete picture of the constituents of the cosmetic matrix, provide an invaluable analytical tool for the quality control of cosmetics, a subject that has been rather neglected to date. © 1997 John Wiley & Sons, Ltd.

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