Anaerobic Digestion Of Swine Manure Research Articles

Comparing the effects of magnetite-mediated direct interspecies electron transfer with biogas mixing-driven interspecies hydrogen transfer on anaerobic digestion

Although the promotive effect of direct interspecies electron transfer (DIET) on methane production has been well-documented, the practical applicability of DIET in different scenarios have not yet been systematically studied. This study compared the effects of magnetite-mediated DIET with conventional biogas mixing-driven interspecies hydrogen transfer (IHT) on anaerobic digestion (AD) of swine manure (SM). Compared with control, magnetite supplementation, biogas circulation, and their integration enhanced the CH4 yield by 19.3%, 25.9%, and 26.2%, respectively. Magnetite mainly enriched DIET-related syntrophic bacteria (Anaerolineae and Synergistia) and methanogens (Methanosarcina) to accelerate acidification and establish DIET, while biogas circulation mainly enriched hydrolytic bacteria (Clostridia) and hydrogenotrophic methanogens (Methanolinea and Methanobacterium) to promote hydrolysis and accelerate IHT. Coupling magnetite addition with biogas circulation led to the enrichment of the above six microorganisms to different extents. The effectiveness of the strategies for lowering the H2 pressure followed: magnetite + biogas circulation ≈ biogas circulation > magnetite. Under stress-free environment, the enhancement effect of magnetite-induced DIET was not even as pronounced as biogas circulation—a simple and common mixing strategy in commercial AD plants, and the promotion effect of magnetite was insignificant in the well-mixed digesters. In short, the magnetite-mediated DIET is not always effective in improving AD of SM.

Microbial analysis of anaerobic digester reveals prevalence of manure microbiota

Anaerobic digestion of swine manure reduces farm greenhouse gases emissions and provides a renewable gas in the agricultural sector. The particular composition of manure, with high concentrations of ammoniacal nitrogen and volatile fatty acids, often threatens the stability of the process through inhibition of methanogens. In this work, continuous production of biogas was tested under relative short hydraulic retention time (15 days). Although a strong inhibition period was detected, adaptation of the microbiota and displacement of bacteria and archaea present in the inoculum by microorganisms present in the animal manure resulted in biogas production close to the values found in standardized batch tests in absence of inhibitions. These findings suggest that in anaerobic digestion of manure, it is not necessary to inoculate, as manure itself contains a large number of active fermentative microorganisms that can even resist long-term digestion inhibition.

Effects of Fe-modified digestate hydrochar at different hydrothermal temperatures on anaerobic digestion of swine manure

Hydrothermal carbonization temperature is a key factor in controlling the physico-chemical properties of hydrochar and affecting its function. In this study, effects of hydrochar and Fe-modified hydrochar (Fe-HC) prepared at 180 °C (180C-Fe), 220 °C (220C-Fe) and 260 °C (260C-Fe) on anaerobic digestion (AD) performance of swine manure was investigated. Among the three Fe-HCs, 220C-Fe had the highest amount of Fe and Fe2+ on the surface. The relative methane production of control reached 174 %-189 % in the 180C-Fe and 220C-Fe treatments between days 11 and 12. The degradation efficiency of swine manure was highest in the 220C-Fe treatment (61.3 %), which was 14.8 % higher than in the control. Fe-HC could act as an electron shuttle, stimulate the coenzyme F420 formation, increase the relative abundance of Methanosarcina and promote electron transport for acetotrophic methanogenesis in the AD. These findings are helpful for designing an efficient process for treating swine manure and utilizing digestate.

Influence of zero-valent iron nanoparticles on anaerobic digestion of swine manure: effects on methane yield

ABSTRACT This study evaluated the effect of zero-valent iron nanoparticles (NZVI) on the anaerobic digestion of swine manure. A wide range of doses of NZVI was evaluated (5, 10, 15, 20, 25, 50, and 100 mgFe°/gVS). The maximum methane yield of 0.4506 L/gVSremoved was obtained with the concentration of 10 mgFe°/gVS representing an increase of 58.99% than the control system with 0.2834 L/gVSremoved, indicating that Fe° improves the methanogenic activity. However, when using doses greater than 20 mgFe°/gVS, there were decreases in the methane yield of 34.4–47.98%. Also, to observe the effect of NZVI in anaerobes was evaluated the activity in the electron transport system (ETS), where the control reactor showed an activity of 31.91 μg INTred/gVS•h, while in reactors with NZVI showed values of 39.48 μg INTred/gVS•h (10 Fe°mg/gVS), observing a stimulation of Fe° in microbial activity. However, the dose of 100 mgFe°/gVS showed the greatest decrease in methane yield (0.1474 L/gVSremoved) and a reduction in ETS was observed by 8.5% compared to the control. The effect on the composition of the volatile fatty acids was observed, where the control system obtained a maximum production of acetic acid of 639 mg/L, which was exceeded with the dose of 10 mg Fe°/gVS by 215% and a decrease of 41.15% with the inhibitory concentration of 100 mg Fe°/gVS. As a result, higher doses of NZVI affect the metabolic activity of anaerobes as well as the acetoclastic pathway causing a decrease in the methane production.

CO2 bubbling pretreatment to remove the inhibition of lime disinfection wastewater in a swine manure anaerobic digestion system

Under the African swine fever epidemic, the extensive use of lime disinfectants has seriously inhibited anaerobic digestion (AD). In this study, the subject was swine manure containing lime disinfectant wastewater. The research set up a carbon dioxide (CO2) bubbling pretreatment device before it entered AD to explore the effect of breaking the inhibition of lime disinfectant. The results showed that the initial pulmonary hypertension (pH) of 11.0 and CO2 bubbling time of 8 h had the best effect, which could reduce calcium ion (Ca2+) by 73.0%, and the biogas production of the batch AD test increased by 28 times. In the continuous AD test, the Ca2+ of CK increased to 7.67 g/L, while the S1 pretreated by CO2 bubbling stabilized at 2.6 g/L, the biogas production in S1 was 112.4% higher than in CK. CO2 bubbling pretreatment reduces the concentration of Ca2+ in swine by utilizing the combination of exogenous CO2 and Ca2+, breaking the inhibitory effect on AD, and thereby improving the performance of the AD system. The test used CO2 in biogas to break the inhibition of lime disinfectants on AD, which was a convenient and feasible pretreatment process.

Biochar facilitates methanogens evolution by enhancing extracellular electron transfer to boost anaerobic digestion of swine manure under ammonia stress

This study explored the mechanisms by which biochar mitigates ammonia inhibition in anaerobic digestion (AD) of swine manure. Findings show 2–8 g/L exogenous ammonia dosages gradually inhibited AD, leading to decreases in the efficiencies of hydrolysis, acidogenesis and methanogenesis by 3.4–70.8%, 6.0–82.0%, and 4.9–93.8%, respectively. However, biochar addition mitigated this inhibition and facilitated methane production. Biochar enhanced microbial activities related to electron transport and extracellular electron transfer. Moreover, biochar primarily enriched Methanosarcina, which, consequently, upregulated the genes encoding formylmethanofuran dehydrogenase and methenyltetrahydromethanopterin cyclohydrolase for the CO2-reducing methanogenesis pathway by 26.9–40.8%. It is believed that biochar mediated direct interspecies electron transfer between syntrophic partners, thereby enhancing methane production under ammonia stress. Interestingly, biochar removal did not significantly impact the AD performance of the acclimated microbial community. This indicated the pivotal role of biochar in triggering methanogen evolution to mitigate ammonia stress rather than the indispensable function after the enrichment of ammonia-resistance methanogen.

Improving removal of combined veterinary antibiotics and mitigating their negative impacts during anaerobic digestion of swine manure using modified bentonite

This study investigated the potential of modified bentonite (MB) material in mitigating the negative effects of combined veterinary antibiotics (CVAs) on anaerobic digestion (AD) process treating swine manure. Four CVAs (i.e., Oxytetracycline (OTC), Tetracycline (TC), Norfloxacin (Norf), and Sulfadiazine (SDZ)) were selected as they are most frequently detected in swine manure; and anaerobically incubated for 30 days under mesophilic conditions (37 °C) and with addition of different MB weights (1, 5, and 10 g L−1). Results showed that 1, 5, and 10 g L−1 of MB enhanced the removal of CVAs from 61.5 (no MB addition) to 72.7, 86.4, and 91.4 %, respectively. CVAs removal was accredited to biodegradation rather than adsorption to MB. Norf was rapidly transferred to solids causing an adsorption competition and decreasing co-antibiotics removal, while it was fast removed when MB was added. When 10 g L−1 of MB were added, the CVAs' adsorption competition diminished and their inhibition on CH4 production decreased from 27 % to 1.7 %. MB addition lowered the impact of CVAs significantly by maintaining pH stability, enhanced COD removal, decreased the VFAs accumulation, and improved the activity of bacteria and archaeal communities. The cost analysis showed that MB additions to AD would reduce cost by 52 % than applied methods for animal slurry pre-treatment or post-digestive treatment for reducing antibiotic residues. These findings suggests that using MB as an environmentally-friendly and commercial additive holds promise for reducing CVAs and eradicating their negative impacts with safe digestate disposal to land applications.

High-throughput profiling the effects of zinc on antibiotic resistance genes in the anaerobic digestion of swine manure

ABSTRACT The problem of antibiotic resistance genes (ARGs) caused by heavy metals has attracted extensive attention of human beings. Zn, a widely used feed additive, has a very high residue in swine manure, but the distribution characteristics of ARGs imposed by Zn in anaerobic digestion (AD) products are not clear. In this study, the behaviour of mobile genetic elements (MGEs), bacterial community, and their association with ARGs were determined in the presence of 125 and 1250 mg L–1 Zn in AD system of swine manure. Zn-treated enriched the abundance of ARGs, and produced some new genotypes that were not detected in CK treatment. In addition, low concentration of Zn significantly increased the relative abundance of ARGs, as compared to higher Zn and CK group. Correspondingly, the abundances of most top30 genus were highest in ZnL (125 mg L–1 Zn), followed by CK and ZnH (1250 mg L–1 Zn). Notably, network analysis showed that the relationship between ARGs and MGEs is closer than that ARGs and bacteria, suggesting that ARGs increased in Zn-treated, especially low level Zn, may be due to the amplification transfer of ARGs among varied microorganisms by horizontal transfer with MGEs. Therefore, strengthen the management of in livestock manure is crucial to control the spread of ARGs in organic fertilizers.

Addition of iron ore tailings to increase the efficiency of anaerobic digestion of swine manure: ecotoxicological and elemental analyses in digestates

Addition of iron ore tailings to increase the efficiency of anaerobic digestion of swine manure: ecotoxicological and elemental analyses in digestates

Influence of Dairy Manure as Inoculum Source on Anaerobic Digestion of Swine Manure.

Inoculation is a widely used method to improve the efficiency of anaerobic digestion (AD) with a high organic load. This study was conducted to prove the potential of dairy manure as an inoculum source for AD of swine manure. Furthermore, an appropriate inoculum-to-substrate (I/S) ratio was determined to improve methane yield and reduce the required time of AD. We carried out 176 days of anaerobic digestion for five different I/S ratios (3, 1, and 0.3 on a volatile solid basis, dairy manure alone, and swine manure alone) of manure, using solid container submerged lab-scale reactors in mesophilic conditions. As a result, solid-state swine manure inoculated with dairy manure could be digested without inhibition caused by ammonia and volatile fatty acid accumulation. The highest methane yield potential was observed in I/S ratios 1 and 0.3, as 133 and 145 mL CH4·g-1-VS, respectively. The lag phase of swine manure alone was more extended, 41 to 47 days, than other treatments containing dairy manure, directly related to tardy startup. These results revealed that dairy manure can be used as an inoculum source for AD of swine manure. The proper I/S ratios leading to successful AD of swine manure were 1 and 0.3.

Evaluation of Potential Substrates for Biogas Production in Colombia using Anaerobic Digestion Systems

Increasing energy demands around the globe require alternative sources of energy. Considering the large amount of agro-industrial and agriculture-related activities in Colombia, energy generation from biomass waste is a promising option to meet the energy needs of the country. Anaerobic digestion (AD) is a good alternative to use these wastes. In this study, several potential substrates for biogas generation using AD systems were identified through a literature review. Vinasses, palm oil industry residues, swine manure, coffee industry residues, and municipal solid wastes were found as potential substrates for AD. Considering factors such as composition, the amount of waste production, availability, and their relationship with important Colombian economic activities, three substrates were selected to perform biochemical methane potential (BMP) experiments. The selected substrates were swine manure (SM), palm oil mill effluent (POME), and coffee residues (CR). The obtained BMP values were 240, 465, and 314 NmLCH4/g VS, respectively. An analysis of kinetic parameters analysis was conducted for the BMP experiments, based on the logistic and Gompertz models. It was seen that the AD of SM starts faster than in the other evaluated substrates. Nevertheless, the overall methane production rate was the highest for POME, followed by CR. SM had the lowest methane production yield. The obtained values of BMP, kinetic parameters, and those collected during the literature review can be useful for the design and implementation of AD systems in Colombia. Moreover, attention should be paid to substrates such as POME, which have a high energy production potential.

Potential of hydrochar/pyrochar derived from sawdust of oriental plane tree for stimulating methanization by mitigating propionic acid inhibition in mesophilic anaerobic digestion of swine manure

VFAs accumulation in anaerobic digestion systems can lead to disturbance of the acid base balance, which has brought major challenges for methane production. Meanwhile, less research explored the potential of biochar derived from wood wastes of oriental plane tree (Platanus orientalis L.) for stimulating methanization in mesophilic anaerobic digestion. In this study, the effects of pyrochar and hydrochar derived from sawdust of oriental plane tree on mesophilic anaerobic digestion of swine manure were compared for the first time. Fourier infrared transform analysis indicated that more functional groups existed on the surface of hydrochar, whereas higher ash content and BET specific surface area were found in pyrochar. The maximum methane production rate during anaerobic digestion was observed in the pyrochar treatment, which increased by 59.5% compared with the control without biochar. Although stimulative effects on dissolved organic carbon and volatile fatty acids production were both observed in the pyrochar and hydrochar treatments, the pyrochar treatment was much easier to trigger multipath methanogenesis and direct interspecific electron transport and subdue propionic acid accumulation compared to the hydrochar treatment. Moreover, redundancy analysis indicated that the variations in acetic acid and dissolved organic carbon were mostly associated with microbial succession. These results suggest that pyrochar has better promoting effects than HC in terms of methane generation and propionic acid inhibition alleviation owing to its special porous structures, functional groups (e.g., C=O, C–O and O–H), and physicochemical properties. These excellent properties play a greater role in recruiting functional archaea and bacteria to regulate the levels of volatile fatty acids and dissolved organic carbon to enhance the methane yield of anaerobic digestion. This study provides novel and valuable information for further engineering applications of pyrochar and hydrochar derived from sawdust of oriental plane tree in energy production and environmental waste treatment.

Biological nitrogen removal mechanisms during anaerobic digestion of swine manure: Effects of biogas circulation and activated carbon addition

This study investigated the biological nitrogen removal mechanisms during the anaerobic digestion of swine manure and the effects of biogas circulation and activated carbon (AC) addition. Biogas circulation, AC addition, and their combination increased the methane yield by 25.9%, 22.3%, and 44.1%, respectively, when compared to the control. Nitrogen species analysis and metagenomic results indicated that nitrification–denitrification was the dominant ammonia removal pathway in all digesters with little oxygen, while anammox did not occur. Biogas circulation could promote mass transfer and induce air infiltration to enrich nitrification- and denitrification-related bacteria and functional genes. And AC might act as an electron shuttle to facilitate ammonia removal. The combined strategies showed a synergetic effect on the enrichment of nitrification and denitrification bacteria and functional genes, significantly lowering the total ammonia nitrogen by 23.6%. A single digester with biogas circulation and AC addition could enhance methanogenesis and ammonia removal via nitrification and denitrification.

Biogas circulation for improving the promotive effect of zero-valent iron on anaerobic digestion of swine manure

Zero-valent iron (ZVI) supplementation for enhancing anaerobic digestion (AD) of swine manure (SM) has been widely studied. However, the low utilization efficiency of ZVI in AD process greatly limits the development of this technology. In this study, a promising approach, biogas circulation was proposed to promote ZVI dissolution during AD. Compared with the control with ZVI only, the CH4 yield was increased by 53.84 % in the digester with ZVI addition and biogas circulation. Biogas circulation apparently increased the system alkalinity and lowered the oxidation-reduction potential through promoting ZVI dissolution and enhancing gas-liquid-solid contact. Particularly, ethanol-type fermentation was favored, as evidenced by the 19.96–183.45 % higher ethanol concentrations in R-Circulation, which might provide more H2 and ethanol to consolidate the hydrogenotrophic pathways and direct interspecies electron transfer (DIET), respectively. Hydrogenotrophic Methanobacterium and Methanothrix that capable of mediating DIET were enriched by 38.81 % and 26.41 % with biogas circulation, respectively.

Alleviating acid inhibition via bentonite supplementation during acidulated swine manure anaerobic digestion: Performance enhancement and microbial mechanism analysis

Swine manure is usually transmitted by the “collection-storage-transport” mode of the biogas project. However, this particular application pattern results in high volatile fatty acids (VFAs) concentration due to the long transition time in the “collection-storage-transport” process. In this work, acidulated swine manure anaerobic digestion (AD) with bentonite supplementation was firstly investigated with an expectation of acid alleviation, performance enhancement and microbial mechanism. Results indicated that the methane production rate in the 20 g/L bentonite-added digester was 2.87 fold higher than that of the control digester. Chemical oxygen demand (COD) removal rate was elevated by 140.1% via bentonite supplementation. Besides, the rapid decrease of VFAs and ammonia indicated that bentonite supplementation could offer buffering capacity and alleviate acid inhibition. Microbial community analysis revealed that acetoclastic methanogenesis (Methanosaeta and Methanosarcina) was the predominant methanogenesis pathway in this AD system. Syntrophic acetate oxidation (SAO) bacteria were discovered in the bentonite-added digester, and they converted acetate into H2/CO2 to support hydrogenotrophic methanogenesis. This study could offer guidance for acidulated swine manure AD in the practical biogas project.

Long-term inhibition of chlortetracycline antibiotics on anaerobic digestion of swine manure

This study aimed to identify whether chronic effects are present in the anaerobic digestion (AD) of swine manure (SM) containing chlortetracycline (CTC), which is one of the major broad-spectrum veterinary antibiotics, and to elucidate the long-term inhibitory effects and recovery from the inhibition based on AD performance and microbial community. Two continuous-stirred tank reactors treating SM with and without CTC spiking (3 mg/L) were operated for 900 days. Due to the degradation and transformation, the total concentration including CTC's epimer and isomer in the test reactor was 1.5 mg/L. The exposure level was determined according to probabilistically estimated concentrations with uncertainties in field conditions. Until the cessation of CTC exposure on day 585, the methane generation of test reactor continuously decreased to 55 ± 17 mL/g-VS/day, 53% that of control. The methane generation and organic removal were not recovered within 300 days after the CTC exposure was stopped. During the experiment, stability parameters such as pH, total ammonium nitrogen, the composition of methane and alkalinity were the same for both reactors. The concentration and composition of VFAs in the test reactor were different with those of control but not in inhibition level. Microbial profiles revealed that reduction in bacterial diversity and changed balance in microbial species resulted in the performance downgrade under the long-term antibiotic pressure. Since it is hard to recover from the inhibition and difficult to predict the inhibition using physicochemical indicators, continuous exposure to CTC needs to be avoided for the sustainable management of AD plants treating SM.

Doubling the Space-Time Yield of a Pilot Biogas Reactor with Swine Manure and Cereal Residues by a Closed Loop Feedback Control Based on an Automated Fuzzy Logic Control System

The anaerobic digestion of swine manure was performed for more than 2 years in a biogas pilot plant with cereal residues as a mono-input, either by a simple intermittent substrate feeding or by feeding with an automated “autopilot” system under the direction of a Fuzzy logic control (FLC) system, working with a closed-loop feedback control. The pilot plant of the University of Applied Sciences in Nordhausen consisted of a 2.5 m3 dosage tank, a 2.5 m3 digestate tank, and a 1 m3 biogas reactor. Only three control parameters were used for FLC: pH, methane %, and the specific gas production rate (GPR) related to the organic loading rate (OLR), that is GPR/OLR m3 biogas/(kgVS d), vs = volatile solids. The specific GPR was referred to the OLR of the last feeding every 8 h in terms of kgVS/(m3 d). In test period I without an FLC system, a safe process with just an OLR of 4 kgVS/(m3 d) was reached, followed by an overloading and reactor disturbance at ≤6.3 kgVS/(m3 d) as indicated by acidification with volatile fatty acids up to 25,000 mg/L. However, test period II (585 trial days) with an integrated FLC system allowed a safe OLR up to 11 kgVS/(m3 d). Apparently, the microbes themselves directed the speed of substrate feeding by the dynamics of their substrate turnover and by the closed loop feedback control, while the three FLC parameters prevented acidification. Therefore, the application of FLC enabled a doubling of the throughput for a biogas reactor in the same time with a ‘turbo speed’. The concomitant hydraulic residence time (HRT) of only 10 days reduced the stirring and heating costs. The usage of an FLC system should open the door for networked biogas production to enable flexible biogas production on demand.

Effect of graphene and graphene oxide on antibiotic resistance genes during copper-contained swine manure anaerobic digestion.

Copper is an important selectors for antibiotic resistance genes (ARGs) transfer because of metal-antibiotic cross-resistance and/or coresistance. Due to carbon-based materials' good adsorption capacity for heavy metals, graphene and graphene oxide have great potential to reduce ARGs abundance in the environment with copper pollution. To figure out the mechanics, this study investigated the effects of graphene and graphene oxide on the succession of ARGs, mobile genetic elements (MGEs), heavy metal resistance genes (HMRGs), and bacterial communities during copper-contained swine manure anaerobic digestion. Results showed that graphene and graphene oxide could reduce ARGs abundance in varying degrees with the anaerobic reactors that contained a higher concentration of copper. Nevertheless, graphene decreased the abundance of ARGs more effectively than graphene oxide. Phylum-level bacteria such as Firmicutes, Bacteroidetes, Spirochaetes, and Verrucomicrobiaat were significantly positively correlated with most ARGs. Network and redundancy analyses demonstrated that alterations in the bacterial community are one of the main factors leading to the changes in ARGs. Firmicutes, Bacteroidetes, and Spirochaetes were enriched lower in graphene reactor than graphene oxide in anaerobic digestion products, which may be the main reason that graphene is superior to graphene oxide in reduced ARGs abundance. Additionally, ARGs were close to HMRGs than MGEs in the treatments with graphene, the opposite in graphene oxide reactors. Therefore, we speculate that the reduction of HMRGs in graphene may contribute to the result that graphene is superior to graphene oxide in reduced ARGs abundance in anaerobic digestion.

Dual character of methane production improvement and antibiotic resistance genes reduction by nano-Fe2O3 addition during anaerobic digestion of swine manure

In this study, the dual character of the methane production and fate of antibiotic resistance genes (ARGs) were evaluated after adding nano-Fe2O3 (NF) during anaerobic digestion (AD) of swine manure, concerning the different exposure times (ET). NF has a decreasing effect on methane production, and the inhibitory effect is proportional to the dosage. NF350 decreased the accumulative methane production by about 7.8% when the ET was 30 days due to the disruption of cellular integrity and the damage to the bacterial communities. Nonetheless, NF could improve the maximum specific methane production rate by up to 24.5% when the ET was shorter than 12 days. And the changes in the relative abundance of ARGs were proportional to the daily methane production at this stage. The addition of NF in a short time is helpful for methanogenic activities, which might be correlated with direct interspecies electron transfer (DIET). NF specifically enhanced the reduction of tetracycline resistance genes with antibiotic target protection (40.7%) and aminoglycoside resistance genes with antibiotic inactivation (34.0%) when the ET was 30 days. It was speculated that the specifical reduction was mainly due to that higher NF could produce the ROS (reactive oxygen species), down regulate key enzyme actions in phosphorylation, acetylization, and nucelotidylalation with antibiotic inactivation, and reduces the reduction of selective pressure for tetracycline. Environmental variables and microbial community were the major factors causing the changes of the ARGs by Structural equation models (SEM) and Procrustes analysis. These results illustrated that resonable NF addition could help to improve the accumulative methane production and reduce ARGs during AD of swine manure.

Energy and Nutrients from Apple Waste Using Anaerobic Digestion and Membrane Technology.

The worldwide increment of food waste requires innovative management solutions, aligned with sustainability, energy, and food security. Anaerobic digestion (AD), followed by nutrient recovery, may be considered an interesting approach. This study proposed a co-digestion of apple pomace (AP) with swine manure (SM) to study the effect of different proportions of AP (0, 7.5, 15, and 30%, on a volatile solids (VS) basis) on the methane production and the stability of the process. Subsequently, the gas-permeable membrane (GPM) technology was applied to recover nitrogen (N) as ammonium sulfate (bio-based fertilizer) from the digestates produced after the AD of 7.5% of AP and SM, and SM alone. The results showed that the co-digestion of 7.5% and 15% of AP with SM presented a methane production similar to the AD of SM alone (with 412.3 ± 62.6, 381.8 ± 134.1, and 421.7 ± 153.6 mL g VS−1 day−1, respectively). The later application of the GPM technology on the resulting digestates, with SM alone and with 7.5% of AP with SM, showed total ammoniacal N recovery rates of 33 and 25.8 g N m−2 d−1, respectively. Therefore, the AP valorization through the AD process, followed by N recovery from the digestate, could be a good management strategy.