Anaerobic Digestion Of Primary Sludge Research Articles

Enhancing Methane Yield in Anaerobic Co-Digestion of Primary Sewage Sludge: A Comprehensive Review on Potential Additives and Strategies

Traditionally, anaerobic digestion has been applied to mixed sludge, combining primary sludge (PS) with secondary sludge. However, recent research has unveiled the advantages of dedicated PS digestion due to its higher energy content. Anaerobic digestion (AD) of primary sewage sludge can offer a sustainable solution for managing sewage sludge while generating renewable energy. The present study provides a comprehensive examination of the current state of knowledge regarding the anaerobic digestion of PS. Co-digestion of PS with organic substrates, including food waste and agro-industrial residues, emerges as a promising approach to boost biogas production. Additionally, the utilization of additives such as glucose and clay minerals has shown potential in improving methane yield. Critical factors affecting AD, such as pretreatment methods, carbon-to-nitrogen (C/N) ratio, temperature, pH, volatile fatty acids (VFAs) levels, organic loading rates (OLR), inoculum-to-substrate ratio (ISR), and the role of additives, have been meticulously studied. Finally, this review consolidates existing knowledge to advance our understanding of primary sewage sludge anaerobic digestion, fostering more efficient and sustainable practices in sludge management and renewable energy generation.

Effect of attapulgite on anaerobic digestion of primary sludge and downstream valorization of produced biosolids

In the light of European Union's Green Deal, the aim of this study was the optimization of municipal primary sludge management, from biomethane production to the final valorization of the produced biosolids. The obtained results showed that the addition of attapulgite in the primary sludge favored the production of methane. The highest methane production was noted when attapulgite was used at a dose equal to 20 g/L. At this dose, methane production raised up to 378 mL CH4/g VS, 47% higher than that of the individual anaerobic digestion of primary sludge. Moreover, in this case, microbial community analysis revealed an increase in the relative abundance of Methanofastidiosaceae and a decrease of Methanocorpusculaceae, suggesting that attapulgite might induce a shift to a more methyl-thiol dominated hydrogenotrophic methanogenesis. In addition, the produced digestate demonstrated better behavior as regards dewaterability, since the normalized capillary suction time was about 54% lower than that of primary's sludge digestate, indicating that attapulgite diminished sludge's capacity to retain water. Finally, it is shown that the dried biosolids amended soil properties by augmenting organic matter content by about 20%, enhancing nutrients bioavailability, while also stimulated plant growth, unveiling their added value in green-based soil improvement solutions.

Impact of primary treatment methods on sludge characteristics and digestibility, and wastewater treatment plant-wide economics

Biogas production from anaerobic sludge digestion plays a central role for wastewater treatment plants to become more energy-efficient or even energy-neutral. Dedicated configurations have been developed to maximize the diversion of soluble and suspended organic matter to sludge streams for energy production through anaerobic digestion, such as A-stage treatment or chemically enhanced primary treatment (CEPT) instead of primary clarifiers. Still, it remains to be investigated to what extent these different treatment steps affect the sludge characteristics and digestibility, which may also impact the economic feasibility of the integrated systems. In this study, a detailed characterization has been performed for sludge obtained from primary clarification (primary sludge), A-stage treatment (A-sludge) and CEPT. The characteristics of all sludges differed significantly from each other. The organic compounds in primary sludge consisted mainly of 40% of carbohydrates, 23% of lipids, and 21% of proteins. A-sludge was characterized by a high amount of proteins (40%) and a moderate amount of carbohydrates (23%), and lipids (16%), while in CEPT sludge, organic compounds were mainly 26% of proteins, 18% of carbohydrates, 18% of lignin, and 12% of lipids. The highest methane yield was obtained from anaerobic digestion of primary sludge (347 ± 16 mL CH4/g VS) and A-sludge (333 ± 6 mL CH4/g VS), while it was lower for CEPT sludge (245 ± 5 mL CH4/g VS). Furthermore, an economic evaluation has been carried out for the three systems, considering energy consumption and recovery, as well as effluent quality and chemical costs. Energy consumption of A-stage was the highest among the three configurations due to aeration energy demand, while CEPT had the highest operational costs due to chemical use. Energy surplus was the highest by the use of CEPT, resulting from the highest fraction of recovered organic matter. By considering the effluent quality of the three systems, CEPT had the highest benefits, followed by A-stage. Integration of CEPT or A-stage, instead of primary clarification in existing wastewater treatment plants, would potentially improve the effluent quality and energy recovery.

Calcium peroxide pre-treatment improved the anaerobic digestion of primary sludge and its co-digestion with waste activated sludge

Primary sludge (PS) and Waste activated sludge (WAS) as two main sludge streams in wastewater treatment plants are commonly anaerobically co-digested, which though may be differently affected by pretreatment. Previous work has found that calcium peroxide (CaO2) pretreatment effectively enhanced anaerobic digestion of WAS. However, the feasibilities of this strategy on PS anaerobic digestion and co-digestion of WAS and PS are still unclear. Herein, this work provided new insights into these systems. Biomethane potential test demonstrated that CaO2 pretreatment at 0.02–0.26 g/g-volatile suspended solids (VSS) promoted anaerobic digestion of PS. Then the feasibility of CaO2 pretreatment for improving anaerobic co-digestion of PS and WAS mixture was confirmed, with the highest improvement in methane production, VSS destruction and sludge reduction being approximately 37.4%, 38.9% and 19.9%, achieved at 0.14 g/g-VSS of CaO2. Process modelling analysis revealed that CaO2 pretreatment increased both degradable faction and actually degraded fraction in sludge mixture. The changes of sludge characteristics via pretreatment and key enzyme activity in sludge anaerobic co-digestion system demonstrated that increased CaO2 concentration resulted in increased soluble organics release from sludge mixture in the pretreatment stage and inhibited activity of coenzyme F420 responsible for methanogenesis. Further mechanism investigation disclosed that OH−, O2− and OH were main contribution factors, and the order of their contributions were OH− >O2− >OH. This work laid the theoretical foundation and provided guidance for the practical application of CaO2 pre-treatment technology.

A new mechanical cutting pretreatment approach towards the improvement of primary sludge fermentation and anaerobic digestion

Pretreatment technologies for improving the anaerobic digestion of waste activated sludge have been studied extensively; however, studies on the improvement of pretreatment technology for primary sludge (PS) fermentation and anaerobic digestion are scarce. In this study, a novel mechanical cutting pretreatment (MCP) method was proposed to enhance the performance of PS fermentation and anaerobic digestion. PS was disintegrated by MCP, the average particle size of PS reduced from 45.7 µm to 7.0 µm, and soluble COD (SCOD) increased by 1206 mg/L with pretreatment for 8 min. The hydrolysis and acidification of PS was promoted significantly by MCP. Compared with the control group, the production of SCOD and volatile fatty acids (VFAs) in the pretreatment group during sludge fermentation increased by 167% and 115.3%, respectively. The performance of PS anaerobic digestion was remarkably improved by MCP. The maximum cumulative biogas production in the pretreatment group was 3153 mL, which was 6.33 times that in the control group (498.5 mL). Sludge disintegration by mechanical pretreatment resulted in the enrichment of bacteria, especially Firmicutes, in the sludge fermentation system, and the quantity of bacteria in the pretreatment group was 1.95 times that in the control group. However, MCP had little effect on archaea in the anaerobic digestion of PS, and the methanogens in the control and pretreatment reactors were 1.493 × 109 and 1.519 × 109 copies/g, respectively.

Effect of type of coagulants on removal efficiency and removal mechanisms of antibiotic resistance genes in anaerobic digestion of primary sludge produced via a chemically enhanced primary treatment process

The potential impact of the trivalent coagulant cations on the removal mechanisms, removal efficiencies and removal patterns of antibiotic resistance genes (ARGs) during anaerobic digestion (AD) of chemically enhanced primary treatment sludge (CEPTS) was investigated using polyaluminium chloride (PACl), ferric chloride (FeCl3) and mixed FeCl3-PACl. The removal efficiency of 23 ARGs and intI1 improved to 72.1% in AD of primary sludge with 100 mg/L FeCl3 and was lowest (only 54.4 %) in AD of primary sludge with 25 mg/L PACl. The removal of ARGs in AD of CEPTS with addition of single or mixed types of Al-based coagulant began to increase rapidly at the onset of batch operation. On the other hand, both the rapid increase in the removal efficiency of ARGs in AD with FeCl3 and the maximum removal efficiency were attained later than in the other ADs.

Denitrification Process Enhancement and Diversity of the Denitrifying Community in the Full Scale Activated Sludge System after Adaptation to Fusel Oil

Implementation of anaerobic digestion of primary sludge in modern wastewater treatment plants (WWTPs) limits the availability of organic carbon for denitrification in conventional nitrification-denitrification (N/DN) systems. In order to ensure efficient denitrification, dosage of the external carbon source is commonly undertaken. However, application of commercial products, such us ethanol or acetate, greatly increases operational costs. As such, inexpensive and efficient alternative carbon sources are strongly desirable. In this study, the use of the fusel oil, a by-product from the distillery industry, was validated in terms of the denitrification process enhancement and impact on the activated sludge bacterial community structure. The experiment was conducted at a full scale biological nutrient removal facility (210,000 PE), in the set of the two technological lines: the experimental line (where fusel oil was introduced at 45 cm3/m3 dose) and the reference line (without an external carbon source addition). During the experimental period of 98 days, conventional nitrate utilization rate (NUR) measurements were carried out on a regular basis in order to assess the biomass adaptation to the fusel oil addition and denitrification process enhancement. While the NURs remained at a stable level in the reference line (1.4 ± 0.1 mg NO3-N/g VSS·h) throughout the entire duration of the experiment, the addition of fusel oil gradually enhanced the denitrification process rate up to 2.7 mg NO3-N/g VSS·h. Moreover, fusel oil contributed to the mitigation of the variability of NO3-N concentrations in the effluent from the anoxic zone. The bacterial community structure, characterized by 16S rRNA PCR—DGGE and the clone libraries of the genes involved in the denitrification process (nirS and nirK), was comparable between the reference and the experimental line during the entire experimental period. In both analyzed lines, the most frequent occurrence of denitrifiers belonging to the genera Acidovorax, Alcaligenes, Azoarcus, Paracoccus and Thauera was noticed. Our results proved that fusel oil would a valuable substrate for denitrification. The addition of fusel oil enhances the process rate and does not reflect a severe selection pressure on the bacterial community at applicable doses. Practical application of fusel oil generates opportunities for the WWTPs to meet effluent standards and reduce operational costs, as well as optimizing waste management for the distillery industry.

Effect of organic loading rate on the biological performance of the thermophilic anaerobic membrane bioreactor treating pulp and paper primary sludge

Waste-to-energy or value-added products have been increasingly considered in many pulp and paper mills (PPMs) worldwide. However, developing appropriate conversion technologies are a major challenge in transforming PPMs wastes into biofuels or value-added biomaterials. In the present study, a long term (320 d) anaerobic digestion of primary sludge of a thermomechanical pulp mill (TMP) was carried out for the first time in a thermophilic anaerobic membrane bioreactor (ThAnMBR). Effect of organic loading rate (OLR) in the range of 2.5–6.8 kg COD/m3 d and hydraulic retention times (HRT) of 3–8 d on the process performance was investigated. Under various OLRs, stable biogas productions were obtained, and the best results were achieved with lower OLR (2.5 kg COD/m3 d) and higher HRT (8 d), at biogas yields of 189 L biogas/kg MLSS fed. However, it was found that the biogas production and sludge biomass degradation decrease when the organic loading rate increases. The proportion of sludge reduction ranged from 28.9 to 46.7% depending on the applied OLRs. Despite varying OLRs, stable membrane performance was obtained, where the required membrane flux was easily maintained during the reactor operation. In this study, also the properties of digestate and membrane permeates were studied under different operating conditions, and they fluctuated to some extent with OLR. ThAnMBR is a promising new technology for pulp and paper mill primary sludge treatment.Graphic abstract

Enhanced Mesophilic Anaerobic Digestion of Primary Sewage Sludge

Processing of the produced primary and secondary sludge during sewage treatment is demanding and requires considerable resources. Most common practices suggest the cotreatment of primary and secondary sludge starting with thickening and anaerobic digestion. The aim of this study is to investigate the anaerobic digestion of the primary sludge only and estimate its impact on sludge treatment and energy recovery. Within this context, the performance of the anaerobic digestion of primary sludge is explored and focused on practices to further enhance the methane production by using additives, e.g., a cationic polyelectrolyte and attapulgite. The results showed that the overall yield in methane production during anaerobic digestion of primary sludge alone was higher than that obtained by the anaerobic digestion of mixed primary and secondary sludge (up to 40%), while the addition of both organic polyelectrolyte and attapulgite enhanced further the production of methane (up to 170%). Attapulgite increased the hydrolysis rate of biosolids and produced relatively stabilized digestate, though of lower dewaterability. Moreover, the results suggest that single digestion of primary sludge may accomplish higher methane production capacities at lower digestors’ volume increasing their overall efficiency and productivity, while the produced digestates are of adequate quality for further utilization mainly in agricultural or energy sectors.

Methane potentials and organic matter characterization of wood fibres from pulp and paper mills: The influence of raw material, pulping process and bleaching technique

During the process of pulp- and papermaking, large volumes of fibre-rich primary sludge are generated. Anaerobic digestion of primary sludge offers a substantial potential for methane production as an alternative approach to the inefficient energy recoveries by commonly used incineration techniques. However, a systematic study of the importance of upstream process techniques for the methane potential of pulp fibres is lacking. Therefore, biochemical methane potentials were determined at mesophilic conditions for 20 types of fibres processed by a variety of pulping and bleaching techniques and from different raw materials. This included fibres from kraft, sulphite, semi-chemical, chemical thermo-mechanical (CTMP) and thermo-mechanical pulping plants and milled raw wood. The pulping technique was clearly important for the methane potential, with the highest potential achieved for kraft and sulphite fibres (390–400 Nml CH4 g VS−1). For raw wood and CTMP, hardwood fibres gave substantially more methane than the corresponding softwood fibres (240 compared to 50 Nml CH4 g VS−1 and 300 compared to 160 Nml CH4 g VS−1, respectively). Nuclear magnetic resonance characterization of the organic content demonstrated that the relative lignin content of the fibres was an important factor for methane production, and that an observed positive effect of bleaching on the methane potential of softwood CTMP fibres was likely related to a higher degree of deacetylation and improved accessibility of the hemicellulose. In conclusion, fibres from kraft and sulphite pulping are promising substrates for methane production irrespective of raw material or bleaching, as well as fibres from CTMP pulping of hardwood.

Microbial Structure and Energy Generation in Microbial Fuel Cells Powered with Waste Anaerobic Digestate

Development of economical and environment-friendly Microbial Fuel Cells (MFCs) technology should be associated with waste management. However, current knowledge regarding microbiological bases of electricity production from complex waste substrates is insufficient. In the following study, microbial composition and electricity generation were investigated in MFCs powered with waste volatile fatty acids (VFAs) from anaerobic digestion of primary sludge. Two anode sizes were tested, resulting in organic loading rates (OLRs) of 69.12 and 36.21 mg chemical oxygen demand (COD)/(g MLSS∙d) in MFC1 and MFC2, respectively. Time of MFC operation affected the microbial structure and the use of waste VFAs promoted microbial diversity. High abundance of Deftia sp. and Methanobacterium sp. characterized start-up period in MFCs. During stable operation, higher OLR in MFC1 favored growth of exoelectrogens from Rhodopseudomonas sp. (13.2%) resulting in a higher and more stable electricity production in comparison with MFC2. At a lower OLR in MFC2, the percentage of exoelectrogens in biomass decreased, while the abundance of genera Leucobacter, Frigoribacterium and Phenylobacterium increased. In turn, this efficiently decomposed complex organic substances, favoring high and stable COD removal (over 85%). Independent of the anode size, Clostridium sp. and exoelectrogens belonging to genera Desulfobulbus and Acinetobacter were abundant in MFCs powered with waste VFAs.

Enhanced anaerobic digestion of primary sludge with additives: Performance and mechanisms

Anaerobic digestion of primary sludge with different additives, namely nano magnetite, graphite powder, activated carbon powder and NiCl2/CoCl2, were evaluated by biomethane potential tests, kinetics modelling and microbial community analysis. Specific methane yields increased from 136 mL/g VS for primary sludge to 146 mL/g VS, 151 mL/g VS, and 152 mL/g VS for the addition of nano magnetite, graphite powder, and activated carbon powder at optimal dosages, respectively. The first order hydrolysis constant kh increased from 0.488 d-1 to 0.526 d-1, 0.622 d-1, and 0.724 d-1, respectively. Microbial community analysis revealed that the abundance of key bacterial and archaeal populations was positively correlated with hydrolysis and methane production. The enhanced methane production with activated carbon powder was due to shifting methane formation pathway from acetoclastic to hydrogenotrophic methanogenesis. In contrast, nano magnetite and graphite powder additives enhanced the direct interspecies electron transfer evidenced by increased abundance of Methanosaeta and Methanolinea.

Enhanced high-quality biomethane production from anaerobic digestion of primary sludge by corn stover biochar

This study conducted batch and continuous tests to reveal the feasibility of corn stover biochar on improving anaerobic digestion of primary sludge (PS). Dosing biochar (1.82, 2.55 and 3.06 g/g Total Solids (TS)) in digester improved methane content increasing from 67.5% to 81.3–87.3% and enhanced methane production by 8.6–17.8%. Model analysis indicated that biochar accelerated PS hydrolysis and enhanced methane potential of PS. The mechanistic studies showed that biochar enhanced process stability provided by strong buffering capacity and alleviated NH3 inhibition. In continuous test over 116 days, the volatile solids (VS) destruction in the biochar-dosed digester increased by 14.9%, resulting in a 14% reduction in the volume of digestate for disposal. Biochar changed microbial community in an expected direction for anaerobic digestion. This work suggests that biochar technology would apply to co-digestion of WAS and PS to maximize the energy recovery and sludge reduction from the two sludge streams.

Comparative study on hydrothermal treatment as pre- and post-treatment of anaerobic digestion of primary sludge: Focus on energy balance, resources transformation and sludge dewaterability

Hydrothermal treatment (HTT) has been recognized as a highly efficient technology for organics decomposition and energy/nutrients recovery from waste biomass. Up to now, however, the efficiency of HTT as the pre- and post-treatment of anaerobic digestion (AD) has not been well compared and documented. In this study, the effects of HTT as the pre- (strategy I, HTT + AD) and post-treatment (strategy II, 1st AD + HTT + 2nd AD) of AD of primary sludge were evaluated based on comparative experiments in regards to energy balance, nutrients transformation, and sludge dewaterability. Results show that the optimal HTT temperature was 130 °C for strategy I and strategy II according to the maximum methane production rate (μ) estimated from Gompertz model and the net energy gain (ΔQ) calculated. Although HTT as the post-treatment of AD achieved higher total methane yield and solids reduction, the increment of methane yield was found to be similar through both strategies compared to their control counterparts (no HTT). The decomposition of insoluble organic carbon was also similar via both strategies. Insoluble nitrogen fraction was detected to be the lowest (6.8%) after HTT at 190 °C in strategy II, comparable to that after HTT at 210 °C (∼10.0%) using strategy I. The proportion of bioavailable phosphorus was found to slightly decrease with the increase of HTT temperature in strategy II, probably due to the alkaline pH in the treated digestate. The sludge dewaterability indicated by specific resistance to filtration (SRF) showed a similar trend after AD under the tested conditions of both strategies, which was remarkably improved when HTT was performed at temperatures higher than 170 °C. HTT as the pre-treatment (strategy I) was found to be more energy efficient in comparison to HTT as the post-treatment (strategy II) of AD of primary sludge. In addition, a positive energy efficiency could be achieved when the sludge solids content ≥2.2%.

The fate of trace organic contaminants during anaerobic digestion of primary sludge: A pilot scale study

A pilot-scale study was conducted to investigate the fate of trace organic contaminants (TrOCs) during anaerobic digestion of primary sludge. Of the 44 TrOCs monitored, 24 were detected in all primary sludge samples. Phase distribution of TrOCs was correlated well with their hydrophobicity (>67% mass in the solid phase when LogD > 1.5). The pilot-scale anaerobic digester achieved a steady performance with a specific methane yield of 0.39–0.92 L/gVSremoved and methane composition of 63–65% despite considerable variation in the primary sludge. The fate of TrOCs in the aqueous and solid phases was governed by their physicochemical properties. Biotransformation was significant (>83%) for five TrOCs with logD < 1.5 and electron donating functional groups in molecular structure. The remaining TrOCs with logD < 1.5 were persistent and thus accumulated in the aqueous phase. Most TrOCs with logD > 1.5 were poorly removed under anaerobic conditions. Sorption onto the solid phase appears to impede the biodegradation of these TrOCs.

The Effect of Short-Term Exposure of Engineered Nanoparticles on Methane Production During Mesophilic Anaerobic Digestion of Primary Sludge

Nanoparticles have been used widely in industry and consumer products in recent years. Most of the engineered nanoparticles (NPs) eventually enter municipal wastewater treatment systems (WWTP) through sewers. In this experimental study, the impact of nano-TiO2, nano-ZnO, and nano-Ag on methanogenesis was investigated during mesophilic batch anaerobic digestion of primary sludge. The experimental sets consisted of 1, 10 mg NP/g TS, and a control group for TiO2 NP, ZnO NP, and Ag NP, separately. The results showed that neither of the NPs used remarkably changed methane production. Methane yields in the units of m3 CH4/kg VS in were between 0.08 and 0.13 and showed no significant difference between the control groups and experimental sets for tested NPs. Soluble Ti concentrations were below 0.07 mg/L after the end of anaerobic digestion. Soluble Zn and soluble Ag concentrations were below 0.78 and 2.02 mg/L, respectively. Most of the NPs remained in the sludge rather than in aqueous supernatant. The authors suggest that the effects of the NPs, just above the sludge, or the NPs that adsorbed to sludge, on methanogenic activity at long-term exposure should be examined in the future studies.

Anaerobic digestion of municipal wastewater sludges using anaerobic fluidized bed bioreactor

The anaerobic digestion of primary sludge (PS) and thickened waste activated sludge (TWAS) using an anaerobic fluidized bed bioreactor (AnFBR) employing zeolite particles as the carrier media was investigated at different organic loading rates (OLRs). PS was tested at OLRs from 4.2 to 39kgCOD/m3-d corresponding to hydraulic retention times (HRTs) from 1.0 to 8.9days. The highest COD removal and VSS destruction efficiencies for primary sludge of 85% and 88%, respectively, were achieved at an HRT of 8.9days and OLR of 4.2kgCOD/m3-d. For TWAS, VSS destruction efficiencies varied from 42% at an HRT of 2.6days and OLR of 13.1kgCOD/m3-d to 69% at an HRT of 8.8days and an OLR of 4.2kgCOD/m3-d. The first-order COD biodegradation rates in the AnFBR for PS and TWAS were 0.4d−1 and 0.1d−1, respectively, almost double the rates in conventional high-rate digesters.

A comparison of the efficacy of organic and mixed-organic polymers with polyaluminium chloride in chemically assisted primary sedimentation (CAPS)

CAPS is the acronym for chemically assisted primary sedimentation, which consists of adding chemicals to raw urban wastewater to increase the efficacy of coagulation, flocculation and sedimentation. The principal benefits of CAPS are: upgrading of urban wastewater treatment plants; increasing efficacy of primary sedimentation; and the major production of energy from the anaerobic digestion of primary sludge. Metal coagulants are usually used because they are both effective and cheap, but they can cause damage to the biological processes of anaerobic digestion. Generally, biodegradable compounds do not have these drawbacks, but they are comparatively more expensive. Both metal coagulants and biodegradable compounds have preferential and penalizing properties in terms of CAPS application. The problem can be solved by means of a multi-criteria analysis. For this purpose, a series of tests was performed in order to compare the efficacy of several organic and mixed-organic polymers with that of polyaluminium chloride (PACl) under specific conditions. The multi-criteria analysis was carried out coupling the simple additive weighting method with the paired comparison technique as a tool to evaluate the criteria priorities. Five criteria with the following priorities were used: chemical oxygen demand (COD) removal > turbidity, SV60>coagulant dose, and coagulant cost. The PACl was the best alternative in 70% of the cases. The CAPS process using PACl made it possible to obtain an average COD removal of 68% compared with 38% obtained, on average, with natural sedimentation and 61% obtained, on average, with the best PACl alternatives (cationic polyacrylamide, natural cationic polymer, dicyandiamide resin).

Previously unclassified bacteria dominate during thermophilic and mesophilic anaerobic pre-treatment of primary sludge

Thermophilic biological pre-treatment enables enhanced anaerobic digestion for treatment of wastewater sludges but, at present, there is limited understanding of the hydrolytic–acidogenic microbial composition and its contribution to this process. In this study, the process was assessed by comparing the microbiology of thermophilic (50–65°C) and mesophilic (35°C) pre-treatment reactors treating primary sludge.A full-cycle approach for the 16S rRNA genes was applied in order to monitor the diversity of bacteria and their abundance in a thermophilic pre-treatment reactor treating primary sludge. For the thermophilic pre-treatment (TP), over 90% of the sequences were previously undetected and these had less than 97% sequence similarity to cultured organisms. During the first 83 days, members of the Betaproteobacteria dominated the community sequences and a newly designed probe was used to monitor a previously unknown bacterium affiliated with the genus Brachymonas. Between days 85 and 183, three phylotypes that affiliated with the genera Comamonas, Clostridium and Lysobacter were persistently dominant in the TP community, as revealed by terminal-restriction fragment length polymorphism (T-RFLP). Hydrolytic and fermentative functions have been speculated for these bacteria. Mesophilic pre-treatment (MP) and TP communities were different but they were both relatively dynamic.Statistical correlation analysis and the function of closely allied reference organisms indicated that previously unclassified bacteria dominated the TP community and may have been functionally involved in the enhanced hydrolytic performance of thermophilic anaerobic pre-treatment. This study is the first to reveal the diversity and dynamics of bacteria during anaerobic digestion of primary sludge.

Application of the Peroxide Regenerated Iron Digester Enhancement (PRI-DE) Technology for Anaerobic Digestion of Primary Sludge

Application of the Peroxide Regenerated Iron Digester Enhancement (PRI-DE) Technology for Anaerobic Digestion of Primary Sludge