Anaerobic Digestion Of Chicken Manure Research Articles

The improvement in methane production, nutrient removal, and heavy metal passivation during anaerobic digestion of chicken manure: The role of modified porous materials

The improvement in methane production, nutrient removal, and heavy metal passivation during anaerobic digestion of chicken manure: The role of modified porous materials

High rate methanogenesis and nitrogenous component transformation in the high-solids anaerobic digestion of chicken manure enhanced by biogas recirculation ammonia stripping

High-solids anaerobic digestion is a promising technology for treating animal manure. However, the high nitrogen content in chicken manure leads to severe ammonia inhibition, thereby hindering the application of this technology for chicken manure. This study operated a high-solid anaerobic system feeding the reactor with 20 % total solids (TS) chicken manure for 650 days to investigate whether the anaerobic metabolic performance could be enhanced by adopting biogas recirculation ammonia stripping. The results show that, with ammonia stripping, the total ammonia nitrogen (TAN) was reduced from 8.2 to 3.0 g/L. The methanogenic activity was enhanced 1.3 – 2.9 fold. This led to a low level of volatile fatty acids (VFA, 0.6 g/L) and a high methane yield of 0.3 L/g-volatile solids (VS). Protein decomposition was facilitated under stripping conditions and correlated with a higher abundance of protein decomposition bacteria and enhanced methane production. The dynamics of ammonium formation and removal were illustrated for different stripping conditions. The ability to maintain low ammonium and the carbon and nitrogen balance was also verified. The proven effectiveness observed in this long continuous experiment may provide a foundation for further demonstrating the high-solids anaerobic digestion of chicken manure.

Genome-centric metagenomics and methanogenic pathway analysis for acclimated anaerobic digestion of chicken manure with high ammonia stressed under thermophilic condition

Thermophilic anaerobic digestion (AD) of animal manure offers various environmental benefits but the process requires a microbial community acclimatized to high ammonia. In current study, a lab-scale continuous stirred tank reactor (CSTR) fed with chicken manure was operated under thermophilic condition for 450 days in total. Results showed that the volumetric methane production decreased from 445 to 328 and sharply declined to 153 mL L−1·d−1 with feeding total solid (TS) step increased from 5% to 7.5% and 10%, respectively. While, after a long-term stop feeding for 80 days, highly disturbed reactor was able to recover methane generation to 739 mL L−1·d−1 at feeding TS of 10%. Isotope analysis indicted acetate converted to methane through the syntrophic acetate oxidation and hydrogenotrophic methanogenesis (SAO-HM) pathway increased from 33% to 63% as the concentration of ammonium increased from 2493 to 6258 mg L−1. Significant different in the genome expression of the SAO bacterial from 0.09% to 1.23%, combining with main hydrogenotrophic partners (Methanoculleus spp. and Methanothermobacter spp.) contented of 2.1% and 99.9% during inhibitory and recovery stages, respectively. The highly expressed KEGG pathway in level 3 (enzyme genes) for the Recovery sludge combining with the extraordinary high abundance of genera Halocella sp. suggested that Halocella sp. might be a highly efficient hydrolytic and acidogenic microorganism and enhance the process of SAO during carbon metabolic flow to methane. This report will be a basis for further study of AD studies on high nitrogen content of poultry manure.

Bioponic Cultivation Using Chicken Droppings to Produce Lettuce Plants (Lactuca sativa rz) Uncontaminated by Trace Metals

Anthropogenic activities have denatured aquatic, terrestrial, and aerial environments throughout the world in general, and in Lubumbashi in particular, where market garden soils have become uncultivable for many plants. Thus, bioponics could be an effective means of producing uncontaminated vegetables in soilless cultivation, not only reducing the amount of fertilizer used and limiting contamination of agricultural produce but also achieving higher yields than in open-ground cultivation. The overall objective of this study was to implement a new bioponic technique for producing liquid fertilizer from chicken manure and utilize it in the organic hydroponic cultivation of lettuce (Lactuca sativa var. Lucrecia) installed on floating raft systems. To achieve this, two types of trials were conducted. The first was aimed at determining the quantities of organic matter to be used in the formulation of nutrient solutions. The second trial aimed to determine the optimal nitrogen concentration to be provided for hydroponic plant growth. Mineralization and/or anaerobic digestion of chicken manure were conducted for 7 days in 200 L barrels. For the first trial, nutrient solutions were created from three different concentrations of chicken manure (0.35%, 3.5%, and 7% dry matter—D.M.). These solutions were then used in bioponic rafts where total ammonia nitrogen (TAN) concentrations were fixed at 150 mg/L. For the second trial, D.M. was fixed at 2.5% for each tested modality, but TAN concentrations varied among them (i.e., 60, 90, and 120 mg/L TAN concentration). Modalities with low D.M. concentration (0.35%) and those with low TAN concentration (60 mg/L) resulted in higher yields than bioponic modalities receiving high concentrations of dry matter or TAN, respectively, for trials 1 and 2. Although the reference chemical solutions generate the greatest yields, bioponic systems operating with chicken manure present a good alternative for the cultivation of vegetables in developing countries with heavily contaminated soils. Indeed, bioponics allows for the production of vegetables in large quantities from animal waste, which does not pose health risks for human consumption. Local vegetable species commonly grown in Lubumbashi should be tested under hydroponic conditions.

Allophane improves anaerobic digestion of chicken manure by alleviating ammonia inhibition and intensifying direct interspecies electron transfer

Synthesized allophane was employed in anaerobic digestion of chicken manure to improve the stability and methane production under ammonia inhibition. Adding 0.5 %, 1.0 % and 1.5 % (w/w) allophane increased the methane production by 261 ∼ 350 % compared with the group without allophane addition. Further investigation indicated that the maximum adsorption capacity of allophane for NH4+-N achieved at 261.9 mg/g; it suggested that allophane adsorption potentially alleviated the ammonia inhibition, which also was reflected by the increase in the activity of the related enzyme, such as coenzyme F420. Moreover, allophane addition also intensified the direct interspecies electron transfer (DIET) in anaerobic digestion; it can be well supported by the increased relative abundance of Methanosaeta and Methanosarcina involved in the DIET. Overall, the improved anaerobic digestion via alleviating ammonia inhibition and intensifying DIET by allophane was elucidated comprehensively, which can contribute to the development of a functional additive for efficient anaerobic digestion in practical application.

Enhancing microbial viability with biochar for increased methane production during the anaerobic digestion of chicken manure

This investigation explores the effects of integrating acid-alkali treated biochar into the anaerobic digestion of chicken manure. Digesters containing chicken manure with biochar addition exhibited an 8.4-fold improvement in methane production compared to untreated digesters. In addition, the porous structure of the biochar promoted microbial acclimation, facilitated direct interspecies electron transfer, and provided anchoring points for immobilisation. Anaerobic digesters containing chicken manure amended with biochar also demonstrated increased chemical oxygen demand removal, up to 81.8 %, and maintained lower total ammonia concentrations (2,478 mg/L) compared to levels in control reactors (4418 mg/L). In terms of the microbial community, the addition of propidium monoazide minimised errors in live microbial population estimation, providing new insights into the capacity of biochar to shield microbes from ammonia stress. These findings highlight biochar's quantitative benefits, emphasising its potential in enhancing methane production, mitigating ammonia stress, and promoting microbial resilience in anaerobic digestion.

Effect of Mixed Antibiotics on Methane Production and Microbial Diversity during Anaerobic Digestion of Chicken Manure

Effect of Mixed Antibiotics on Methane Production and Microbial Diversity during Anaerobic Digestion of Chicken Manure

The key metabolic pathway for enhanced anaerobic digestion of chicken manure with coal slime for methane production

Coal slime can significantly enhance anaerobic digestion (AD) of chicken manure (CM) for methane production; However, the underlying mechanism is still unclear. This study has mainly discussed variation characteristics of key metabolic pathways in the AD process of CM by coal slime. Dry CM and coal slime (CS) were selected and coal seam mine water was applied as bacteria source. CS effects on the metabolic pathway of CM fermentation system were studied by biogas production simulation experiments and metagenomic analyses. The results indicated that variations in pH and CS amount exerted significant effects on the biogas generation in the contest of CM fermentation. Optimal conditions for enhanced biogas production were observed when the pH approached neutrality, and the CS quantity was 0.2 g, highlighting substantial potential of CM for biogas generation. The addition of CS resulted in a 13.1%, 1.9%, and 1.8% increase in the relative abundance of Bacteroides, Aminobacterium, and Methanothrix. This, in turn, elevated gene abundance of essential enzymes in the glycolytic pathway (glycolysis and pyruvate metabolism), promoting acetic acid production and providing a foundational basis for methanogen metabolic gas production. Furthermore, the addition of CS lead to a notable increase in the abundance of the pilA gene in Methanocorpusculuma (111.5%) and Methanothrix (66.1%). This suggested the involvement of CS in DIEF, thereby augmenting the anaerobic methane production potential of CM. These outcomes offer valuable theoretical insights for optimizing the biogas production potential of CM and maximizing the resource utilization of CS.

Enhanced methane production during the anaerobic digestion of chicken manure through the addition of pristine and recovered biochar

This study investigates the impact of a 50:50 mixture of pristine and recovered biochar (CBC) on high-solids anaerobic digestion of chicken manure, comparing its performance with pristine biochar (PBC), recovered biochar (RBC), and a Control group. The experiment, conducted under mesophilic conditions using a 15% total solid content and 5% biochar dosage, showed that CBC treatment achieves the highest cumulative biogas production (314 mL g−1-VS) and the highest cumulative methane production o(112 mL g−1-VS) over 21 days. Further, digesters containing CBC exhibited the highest rate of COD removal (80.8%), surpassing digesters amended with PBC (71.1%), RBC (69.2%) and Control (57.8%). Although digesters amended with PBC showed a higher TAN adsorption capacity of 48 mg g−1 compared to the 32 mg g−1 of CBC, both TAN adsorption and the existing microbial population of 1.2 × 1014 microbes per gram, have allowed CBC to exhibit superior digester performance. These findings offer valuable insights for optimising anaerobic digestion processes and cutting operational costs in waste-to-energy projects incorporating biochar as an additive.

Applying current-carrying-coil-based magnetic field (CCC-MF) to promote anaerobic digestion of chicken manure: Performance evaluation, mitigation of ammonia inhibition, microbial community analysis, and pilot-scale validation

This study aims to investigate the effectiveness of a current-carrying-coil-based magnetic field (CCC-MF) placed outside the digester in promoting anaerobic digestion (AD) of chicken manure through digester performance evaluation, microbial community analysis, and pilot-scale experiments. With the applied CCC-MF, a magnetic field of 1.12–3.54 mT was generated in the anaerobic digester, and a 40% average methane yield increment was obtained from the AD of chicken manure. AD experiments showed that CCC-MF increased the average CH4 percentage by 18.7% and decreased the average CO2 percentage by 22.9%. The ammonia nitrogen concentration was decreased by 10.4–64.9% due to CCC-MF, indicating that CCC-MF was beneficial to the mitigation of ammonia inhibition. The activities of acetate kinase and F420 were increased by 21.9% and 30% due to CCC-MF. Microbial community analysis indicated that the dominant bacterial phyla were Thermotogae, Firmicutes and Bacteroidetes while the dominant methanogenic archaeal genera were Methanoculleus, Methanosarcina, Methanothrix, Methanomassiliicoccus, Methanolinea, and Methanospirillum. Due to CCC-MF, the electrotrophic methanogenic genus Methanothrix and ammonia-tolerant methanogen genus Methanosarcina were enriched by 2.1-fold and 1.6-fold, respectively. The KEGG analysis of metabolic pathways showed that many key metabolic pathways were enhanced 9–20 times due to CCC-MF. Furthermore, the results of pilot-scale validation experiments confirmed the technical effectiveness of the CCC-MF assistance in enhancing AD of chicken manure. This study provides a novel and promising strategy by placing a current-carrying coil outside the AD system for enhancing methane production from AD of animal manure and reveals the enhancing mechanisms of the technology.

Wood biochar enhances methanogenesis in the anaerobic digestion of chicken manure under ammonia inhibition conditions

The process of breaking down chicken manure through anaerobic digestion is an effective waste management technology. However, chicken manure can be a challenging feedstock, causing ammonia stress and digester instability. This study examined the impacts of adding wood biochar and acid-alkali-treated wood biochar to anaerobically digest chicken manure under conditions of ammonia inhibition. The results highlighted that only the addition of 5 % acid-alkali-treated wood biochar by volume can achieve cumulative methane production close to the typical methane potential range of chicken manure. The treated wood biochar also exhibited highest total ammonia nitrogen removal compared to the Control treatment. Scanning Electron Microscope revealed growing interactions between biochar and methanogens over time. Real-time polymerase chain reaction showed that treated wood biochar produced the highest number of bacterial biomass. In addition, 16S amplicon-based sequencing identified a more robust archaeal community from treated biochar addition. Overall, the acid-alkali treatment of biochar represents an effective method of modifying biochar to improve its performance in anaerobic digestion.

Generation of High Concentration Free Volatile Fatty Acids from Continuous Anaerobic Digestion of Chicken Manure by Suppressing the Decomposition of Nitrogenous Components.

Free volatile fatty acids (free VFA) play a crucial role in the inactivation of pathogens during the anaerobic digestion of animal manure. However, the decomposition of nitrogenous components can release alkaline ammonium-N, which might increase the pH and reduce the concentration of free VFA. In this study, continuous anaerobic digestion of high-solid chicken manure was conducted for 150days. The results indicated the process stabilized at a pH of approximately 6.0, with total ammonia nitrogen (TAN) of around 7.0g/L. The resulting concentration of free VFA was only about 3.1g/L, which might not sufficiently effective for pathogen inactivation. On the 70th day, hydrogen chloride was added into the reactor to adjust the pH to 5.5. This led to a further decrease in pH to 4.3 and TAN to 2.3g/L. As a result, the concentration of free VFA significantly increased, reaching up to 12.6g/L. These findings support the potential for generating high levels of free VFA even for nitrogen-rich manure by implementing an appropriate process regulation.

Biosolids-Derived Biochar Improves Biomethane Production in the Anaerobic Digestion of Chicken Manure

Anaerobic digestion has attracted great interest for use in the management of organic wastes and the production of biomethane. However, this process is facing challenges, such as a high concentration of ammonia nitrogen, which affects the methanogenesis process and, thus, the production of methane. This study investigates the use of biosolid-derived biochar for mitigating ammonia stress and improving methane production during the anaerobic digestion of chicken manure, using both pristine biochar and biochar modified with a potassium hydroxide (KOH) solution. Batch mesophilic anaerobic digestion (37 °C) was carried out over 18 days. When compared to chicken-manure-only controls, a significant increase in methane formation was observed in the digesters amended with biochar and KOH-modified biochar, producing 220 L kg−1 volatile solids (VSs) and 262 L kg−1 VSs of methane, respectively, compared to 139 L kg−1 VSs from the control digesters. The use of biochar and KOH-modified biochar resulted in a significant reduction of 8 days in the lag phase. The total ammonia nitrogen (TAN) concentration was reduced in the digesters with biochar and KOH-modified biochar by 25% and 35.5%, respectively. The quantitative polymerase chain reaction (QPCR) data revealed that the number of 16S rRNA gene copies was around 50,000 and 41,000 times higher in the biochar and KOH-modified biochar digesters, respectively, compared to the control digesters on day 18. The taxonomic profiles indicated that the BC and KOH-BC digesters contained a mixture of methanogenic pathways, including acetoclastic (Methanosaetaceae), hydrogenotrophic (Methanosarcinaceae), and methylation (Methanofastidiosaceae). This mix of pathways suggests a more robust archaeal community and, hence, more efficient methanogenesis. The results show that the addition of biosolids biochar enhances anaerobic digestion, mitigates ammonia stress to methanogens, and significantly increases biogas production.

Process Performance and Functional Microbial Community in the Anaerobic Digestion of Chicken Manure: A Review

Anaerobic digestion is one of the most widely used treatment methods for animal manure. Chicken manure has high methane production potential and is thus a suitable substrate for biogas plants. However, high nitrogen content inhibits the metabolism of anaerobic microorganisms and thus hinders methane production from chicken manure. Enhancing the performance of anaerobic digestion for chicken manure is indeed a long-standing challenge. This review presents new insights into maintaining methanogens’ activities, the decomposition of acetate, and the dynamics of methanogenic pathways under high ammonia stress. This review also analyzed the possible strategies for alleviating ammonia inhibition effects, including supplementing trace elements, co-digestion with nitrogen-less materials, in-situ ammonia removal, and long adaptation of anaerobic consortia to ammonia stress. The insights obtained in this paper may provide helpful information for a better understanding of anaerobic digestion technology for chicken manure and other nitrogen-rich waste and wastewater.

Control Strategies for Enhanced Biogas Production from Chicken Manure

AbstractThe control strategies adopted in formulation and anaerobic digestion (AD) process of chicken manure (CM) for the control and enhancement of biogas production are reviewed. The existing limitations and future challenges in the production of biogas from AD of CM are briefly discussed. The control strategy adopted for formulation is pretreatment of CM (physical, chemical, biological, and thermal) while strategies for AD include selection of optimal parameters (pH, C/N ratio, organic loading rate (OLR), and temperature), co‐AD with other substrates, and addition of supporting materials. The pretreatment of CM, selection of optimal parameters, Co‐AD of CM with other substrates, and addition of supporting materials overall enhance biogas and methane production due to increase of hydrolysis and C/N and decrease of ammonia inhibition, CO2 and H2S contents. The implementation of developed control strategies in continuous plants, development of new strategies, and use of supporting materials in combinations of two or three for better performance are the future challenges in the AD of CM. The control strategies had a significant impact on the efficient use of CM and improved biogas and methane production.

Co-anaerobic digestion of sawdust and chicken manure with plant herbs: Biogas generation and kinetic study

Plant herbs specifically serai wangi (SW) and peppermint (PPM) are selected for its insect repellent properties as the use of chicken manure (CM) in anaerobic digestion (AD) potentially attract flies due to the digestate produced. Hence, the addition of SW and PPM in the AD system of CM could deter flies' infestation while producing biogas. Previous work has shown that AD of sawdust (SD) and CM with these plant herbs were able to produce biogas and reduce the flies attraction towards the digestate. However, the combination of SW and PPM for AD of CM has yet to be investigated. This work describes the effect of mixing SW and PPM on the co-AD of SDCM with respect to biogas production, methane yield and kinetic analysis. The mixture of SW and PPM was varied at different concentrations. The composition of methane in biogas was characterized every 10 days by using gas chromatography (GC) equipped with a thermal conductivity detector (TCD). The results suggest that co-AD of 10SW10PPM exhibited the highest biogas production (52.28 mL/gvs) and methane yield (30.89 mL/gvs), which the purity of methane increased by 18.52% as compared to SDCM. However, increasing the concentration of SW and PPM does not significantly improve the overall process. High R2 (0.927–0.999), low RMSE (0.08–0.61) and low prediction error (<10.00%) were displayed by the modified Gompertz, logistic and Cone models. In contrast, Monod and Fitzhugh model is not preferred for the co-AD of SDCM with a mixture of SW and PM, as a high prediction error is obtained throughout the study. Increasing the dosage of PPM decreases the maximum cumulative methane yield, ranging from 31.76 to 7.01 mL/gvs for modified Gompertz and 89.56 to 19.31 mL/gvs for logistic model. The Modified Gompertz obtained a lag phase of 10.01–28.28 days while the logistic model obtained a lag phase of 37.29–52.48 days.

Optimization of substrate mixing ratios for the anaerobic digestion of chicken manure and slaughterhouse waste: a case study of Irvine’s Zimbabwe (Pvt) Ltd, Zimbabwe

This study investigated the biogas potential of co-digesting chicken manure (CM) and slaughterhouse waste (SW) at Irvine’s Zimbabwe (Pvt) Ltd farm. Four different mixes of CM to SW as a percentage of total solids (TS) as follow: Mix 1 (100%: 0%); Mix 2 (67%: 33%); Mix 3 (33%: 67%) and Mix 4 (0%: 100%) were considered for co-digestion using cow dung as inoculum. The mixes were characterised in terms of carbon to nitrogen (C/N) ratio, chemical oxygen demand (COD), pH, TS, and volatile solids (VS). After 15 days of digestion, Mix 1 produced the highest biogas of 51 mL/gTS (24.4 m3/m3 feedstock) with a methane content of 30.2 mL/gTS (59.1%). The COD, pH, and TS values for the mixes after digestion ranged from 11,250 mg/L (Mix 1) to 25,000 mg/L (Mix 4), 5.54 (Mix 1) to 6.48 (Mix 4) and 11.5% (Mix 4) to 14.7% (Mix 2), respectively. Unlike findings from other studies that suggest that co-digestion increases gas production, mono-digestion of CM had the highest biogas potential and produced the most stable sludge. Therefore, mono-digestion of CM can be used for biogas production at Irvine’s Zimbabwe (Pvt) Ltd farm.

Integrated omics analysis on the bacterial community in the anaerobic digestion of chicken manure materials in biogas production farm

The overall effectiveness of anaerobic digestion is affected by the initial three phases as a result of substrates created for the methanogens to take in. In this study, the bacterial composition of the various chambers in the anaerobic digestion of chicken manure was investigated. Diversity of the microbial community for chicken manure was relatively simpler, consisting mainly of Lactobacillus, compared to the main digestion chambers with a more complex community. Chicken manure only has hydrolysis phase occurring, while the main digesters have the first three phases of anaerobic digestion concurrently occurring. A comparison of the metagenomics versus metaproteomics analysis revealed that the main difference was in the percentage of unknown microorganisms. The quantity of volatile fatty acids detected in each respective chamber suggested the effectiveness of the microorganisms in assimilating the metabolites. Successful integration of these study modes would present a better understanding on anaerobic digestion of complex systems.

Influence of nano-Fe3O4 biochar on the methanation pathway during anaerobic digestion of chicken manure

Volatile fatty acids and ammonia nitrogen (AN) accumulate during anaerobic digestion (AD) of high N substrates, such as chicken manure (CM), causing decreases in methane yield. Previous research found that the addition of nano-Fe3O4 biochar can alleviate the inhibition caused by acids and ammonia and increase methane production. The mechanism of enhanced methane production in nano-Fe3O4 biochar-mediated AD of CM was explored in depth in this study. The results showed the lowest AN concentration in the control and nano-Fe3O4 biochar addition groups were 8,229.0 mg/L and 7,701.5 mg/L, respectively. Methane yield of volatile solids increased from 92.0 mL/g to 219.9 mL/g in the nano-Fe3O4 biochar treatment, which was attributed to the enrichment of unclassified Clostridiales and Methanosarcina. The mechanism of nano-Fe3O4 biochar in AD of CM under high AN level was to improve methane production by promoting syntrophic acetate oxidation and facilitating direct electron transfer between microorganisms.

Effect of Temperature on Co-Anaerobic Digestion of Chicken Manure and Empty Fruit Bunch: A Kinetic Parametric Study

The rapid growth of the Malaysian poultry and palm oil industries has led biomass waste generation in abundance specifically chicken manure and empty fruit bunch (EFB). Anaerobic digestion (AD) is a circular economy-based approach which converts chicken manure and EFB into biogas which can be utilized for heating and power generation. Operating temperature is an imperative consideration for AD hence the objective of the study is to evaluate the effect of different temperature profiles namely, psychrophilic (20 °C), mesophilic (35 °C) and thermophilic (50 °C) on AD of chicken manure and EFB. The kinetic parameters are also evaluated using five kinetic models to enable readers to comprehend the kinetic behaviours of the systems. The volume and composition of biogas is measured every five days for a 50-day retention time. The findings observed that mesophilic condition is the most favourable with cumulative methane, CH4 composition of up to 17.07%, almost two folds that of thermophilic (9.12%) and five folds that of psychrophilic (3.49%). The CH4 generation rate, Rb based on the modified Gompertz model which is deemed the best fit further supports these findings as the Rb under mesophilic condition is significantly higher (0.330 mL/gvs day) compared to psychrophilic (0.088 mL/gvs day) and thermophilic (0.120 mL/gvs day) conditions.