The asphalt binder used in pavements oxidizes quickly owing to the elevated temperatures, solar radiation, and exposure to atmospheric oxygen. In this study, epoxidized castor oil (ECO) and acrylated cashew nut shell liquid (ACNSL) were investigated as potential rejuvenators for use as aged binders. The binders were aged in the laboratory using short- and long-term aging methods. The additives and rejuvenated binders were characterized by Fourier transform infrared spectroscopy (FTIR). The properties of the rejuvenated binders were evaluated by frequency sweep, multiple stress creep and recovery (MSCR), and linear amplitude sweep (LAS) tests. The additives improved binder performance, particularly for the sample modified with 15 % cashew nut shell liquid (CNSL). The rheological parameters indicated that both the bio-rejuvenators increased the elasticity of the binder. Regarding the rutting resistance as measured by MSCR test, the results indicated that the sample with the 7 % ACNSL rejuvenated binder exhibited the best rutting resistance at both intermediate and elevated temperatures. Additionally, the fatigue resistance of the sample with 10 % ACNSL was higher than those of the other samples. This study indicates that the bio-oils used are potentially useful as rejuvenating agents for aged binders, thus enabling the recycling of aged asphalt binders.