Clarifying the low-frequency vibration transfer law of fruit trees is an important prerequisite for studying the intelligent harvesting operation of a low-frequency shaker. Through a vibration transmission test involving vibration parameters, tree parameters, and proportional acceleration, this study found that vibration frequency, amplitude, pre-tension, and rope length were the most significant influencing factors of proportional acceleration. Among them, amplitude and pre-tension have positive effects on proportional acceleration, vibration frequency and rope length have negative effects on proportional acceleration, and the interaction between amplitude and vibration frequency has significant effects on proportional acceleration. The proportional acceleration has a maximum response of 8.12m/s2 when the vibration frequency is 6.86Hz, the amplitude is 64.92mm, the pre-tension is 1007.02N, and the rope length is 6m. The results of the study can guide research on and development of a low-frequency intelligent shaking machine as well as its field operation.