Amount Of Nitrogen Fertilizer Research Articles

Does the Amount of Pre-Sowing Nitrogen Fertilization Affect Sugar Beet Root Yield and Quality of Different Genotypes?

There has always been a specific focus on nitrogen fertilization in sugar beet production due to its important effect on sugar beet root yield and quality. For stable sugar beet growth and satisfactory root yield and quality, balanced N fertilization is crucial. Thus, this study aimed to investigate spring N fertilization in two seasons as the following treatments: N0—control, N1—only pre-sowing fertilization, and N2—pre-sowing with topdressing. Four different genotypes were included in the study (Serenada, Colonia, Fred, and Danton). The experiment was set up in a plain area, belonging to the temperate climate zone in Eastern Croatia (Županja and Vrbanja), with the long-term mean (LTM) (March–October) air temperature around 16 °C and the total precipitation of 515 mm. Pre-sowing N fertilization had a smaller impact on root yield in the year with higher precipitation (31% higher than LTM). Therefore, the average yields with pre-sowing fertilization (N1) and pre-sowing fertilization with top dressing (N2) were very similar and were only 7% higher than those of the control. In a season with less rainfall (29% less than LTM), pre-sowing fertilization with top dressing (N2) had a more pronounced effect on the increase in sugar beet root yield, which was 17% higher compared to that of the control treatment. The sugar beet sucrose content and quality parameters (brei impurities, loss of sugar in molasses, extractable sugar) differed when N fertilization was applied among locations in both seasons. The white sugar yield was the highest at N2 treatment with pre-sowing and topdressing N fertilization. In general, according to the average of all locations and years of research, the Serenada hybrid achieved the highest average root yield (81.1 t ha−1), while Colonia exhibited the highest root sugar content (14.5%) and white sugar yield (9.7 t ha−1).

Environmental transport of excess nitrogen fertilizer in peach orchard: Evidence arising from 15N tracing trial

In pursuit of high fruit yield, overwhelming amount of nitrogen (N) fertilizer was unreasonably applied in orchard, resulting in lower fertilizer N uptake and higher environmental loss. It is crucial to propose appropriate fertilization timing and optimal N management by clarifying fruit tree N uptake pattern in orchard systems. Here, a 15N isotope enrichment trial was conducted in peach orchard to assess the transport of applied synthesized fertilizer-N to the plant and environment in the Taihu Lake region. Ammonium sulfate ((NH4)2SO4, 10.12 atom % 15N), as the N source, was applied with four split applications at 382.5 kg N ha−1 during one plant growth cycle. Results showed N deriving from fertilizer (NDFF) accounted for 24.5% in leaves and 38.4% in fruits, respectively. During the annual growth cycle, the applied fertilizer-N uptake and utilization was higher in reproductive growth stage than vegetative growth stage. After harvest, 28.8% of applied fertilizer-N was absorbed by peach tree, 9.5% was lost to the environment through surface runoff, ammonia (NH3) volatilization and nitrous oxide (N2O) emission, 29.6% was retained in the 0–160 cm soil profile, the remaining 32.1% was unaccounted N including N leaching and N loss from the N removal progress. Generally, more applied fertilizer-N moved to the environment in high N input peach orchard. In order to promote optimal N management, the applied fertilizer-N rate should be reduced by 30% and emphasized on fertilization at the reproductive growth period according to tree N uptake pattern.

Low nitrogen priming improves nitrogen uptake and assimilation adaptation to nitrogen deficit stress in wheat seedling.

Early-stage low nitrogen priming promotes root growth and delays leaf senescence through gene expression, enhancing nitrogen absorption and assimilation in wheat seedlings, thereby alleviating growth inhibition under nitrogen deficit stress and supporting normal seedling development. Verifying the strategies to reduce the amount of nitrogen (N) fertilizer while maintaining high crop yields is important for improving crop N use efficiency (NUE) and protecting the environment. To determine whether low N (LN) priming (LNP) can alleviate the impact of N-deficit stress on the growth of wheat seedlings and improve their tolerance to N-deficit stress, we conducted hydroponic experiments using two wheat cultivars, Yangmai 158 (YM158, LN tolerant) and Zaoyangmai (ZYM, LN sensitive) to study the effects of LNP on wheat seedlings under N-deficit stress. N-deficit stress decreased the plant dry weight, leaf area, and leaf N content (LNC), while LNP could significantly reduce this reduction. Distinct sensitivities to N-deficit stress were observed between the wheat cultivars, with ZYM showing an early decrease in leaf N content compared to YM158, which exhibited a late-stage reduction. LNP promoted root growth, expanded N uptake area, and upregulated the expression of TaNRT1.1, TaNRT2.1, and TaNRT2.2 in wheat seedlings, suggesting that LNP can enhance root N uptake capacity to increase N accumulation in plants. In addition, LNP improved the activity of glutamine synthase (GS) to enhance the capacity of N assimilation of plants. The relative expression of TaGS1 in the lower leaves of priming and stress (PS) was lower than that of no priming and stress (NS) after LNP, indicating that the rate of N transfer from the lower leaves to the upper leaves became slower after LNP, which alleviated the senescence of the lower leaves. The relative expression of TaGS2 was significantly increased, which might be related to the enhanced photorespiratory ammonia assimilation capacity after LNP, which reduced the N loss and maintained higher LNC. Therefore, LNP in the early stage can improve the N absorption and assimilation ability and maintain the normal N supply to alleviate the inhibition of N-deficit stress in wheat seedlings.

GENERATING NITROGEN RECOMMENDATIONS FOR MAIZE: ECONOMIC CONSIDERATIONS

The use of excessive amounts of nitrogen fertilizers in crops usually results in nitrogen (N) losses, increasing environmental pollution and contributing to climate change, while also elevating crop production costs. This study aimed to determine in maize (Zea mays L.): (1) the economic optimal nitrogen rate (EONR), economic optimal yield (EOY), and N use efficiency (NUE) utilizing the current N/maize (grain) price ratio (N/MPR) for attainable yield levels; and (2) the effect of an increase in the N/MPR on the EONR, EOY, net income (NI), NUE, and the loss of N. Data from 140 maize response experiments to increasing rates of N, conducted between 2010 and 2019 across 18 states in Mexico under rainfed and irrigated conditions, were analyzed. In these experiments, the average maximum yields were 4.971 ± 2.716 Mg ha-1 under rainfed conditions and 11.776 ± 2.366 Mg ha-1 under irrigated conditions. The EONR, EOY, and NUE were determined with the current N/MPR for attainable yield levels. At these levels, both the EONR and NUE increased with higher EOY and decreased with increased yield without N treatment. An increase in N/MPR reduced EONR and its associated costs without significantly affecting EOY and NI. The reduction in EONR resulted in higher NUE and, consequently, lower N losses. By utilizing N/MRP in economic optimization, recommended N rates for maize can be reduced, leading to decreased N losses and environmental pollution.

Effects of Different Proportions of Organic Fertilizer Replacing Chemical Fertilizer on Soil Nutrients and Fertilizer Utilization in Gray Desert Soil

Organic fertilizer can improve soil management and alleviate soil nutrient loss caused by excessive fertilization. This study determines a fertilization scheme that can achieve high and stable crop yield and effective soil fertilization by exploring the effects of different organic fertilizer proportions on soil nutrient content, fertilizer utilization rate, and wheat yield. The experiment was conducted from 2018 to 2020 using the Xinchun 38 wheat variety and gray desert soil. The experiment used six treatments: no fertilizer (CK), with normal nitrogen and phosphorus fertilizer (CF). The amount of conventional chemical nitrogen fertilizer was reduced by 6%, 12%, 18%, and 24%, respectively, and supplemented with organic nitrogen fertilizer of the same proportion. The experimental treatment codes were SF6, SF12, SF18, and SF24, respectively. Plant samples from six wheat growth stages and 0–20 cm soil samples were collected to analyze the nitrogen and phosphorus content and organic matter; dry matter accumulation, crop yield, and yield components were measured at the harvest stage. Soil available nitrogen, phosphorus, potassium, and soil organic matter contents increased with the replacement ratio of organic fertilizer after three consecutive years of application. The available nutrients and organic matter in soil treated with SF18 and SF24 were significantly higher than those in CK and CF. The dry matter accumulation and nutrient accumulation of wheat increased with increasing organic fertilizer replacement rates of SF18 and SF24, respectively. Nitrogen and phosphorus fertilizer utilization rates, partial productivity, and agricultural use efficiency also increased, with the SF18 and SF24 treatments having higher organic fertilizer replacement rates than those in the other treatments. The number of ears, 1000-grain weight, and yield of wheat treated with SF18 treatment produced the best results. Therefore, continuously using organic fertilizer as a partial replacement for conventional fertilizer can increase wheat growth and soil nutrient availability, which can improve the utilization rate of fertilizer, thereby achieving stability and even a significant increase in yield.

Effect of Three Different Nitrogen Rates and Three Rhizosphere N2- Fixing Bacteria on Growth, Yield and Quality of Peanuts

Raising prices of organic fertilizers, environmental relation of their usage and need for global foodstuff, placed a topic of universal interest in cultivation of legume plants for human nutrients and soil fertility supplementation. Three rates of N fertilizer were studied in the field: 0, 20 and 40 kg N/ha. Three bacterial strains were identified and inoculated in the experiment: Enterobacter asburiae (E. asburiae), Klebsiella quasipneumoniae (K. quasipneumoniae) and Enterobacter cloacae (E. cloacae). The soil samples were taken before and after experiment for chemical attributes analysis. The effect of N2 fertilizer and 3 bacterial strains was on soil chemical properties, yield components and yield of peanut plant. The results of present study showed that application of different N2 fertilizer rates was a little influence on the N2-fixing ability of rhizosphere bacteria, but high effect was significant at the peanut nodule number. In the 3 N fertilizer rates and 3 bacteria, which only had N2 fertilizer rate of 40 kg N/ha and E. asburiae significantly raised the maximum yield component and yield of peanut, while there was the lowest yield component and yield of peanut was observed in N fertilizer rate application of 0 kg N/ha and E. cloacae inoculation. The differences in rhizosphere bacterium strains between different N fertilizer rates was better than that caused by the nitrogen fertilizer amount. This could be due to the substantial improvement in soil chemical attributes at different soils, especially in, pH, OM and total N, as these significantly impacted the soil structure. HIGHLIGHTS Increasing pH, organic matter and total nitrogen of soil were statistically significantly different by 3 N2 fertilizer rates. There is a positive interaction between nitrogen rates and RNFB increasing yield of peanut. Number and weight of peanut nodules obtained the maximum value without applying N2 application compare to other treatments. The maximum yield components of peanut were inoculated with asburiae to compare with K. quasipneumoniae and E. cloacae and applied weight of 40 kg N/ha. The maximum yield of peanut was inoculated with asburiae to compare with K. quasipneumoniae and E. cloacae. GRAPHICAL ABSTRACT

Water use and carbon assimilation respond differently to nitrogen supplementation in mid-rotation Eucalyptus nitens

ABSTRACT Large amounts of nitrogen (N) fertiliser are often applied to commercial plantations in southern Australia to obtain high growth rates. The growth response to fertiliser can be realised through increased foliar N and hence improved leaf-level photosynthetic capacity, or through increased leaf area production, which in turn increases light capture and hence canopy-level photosynthesis. While extra leaf area is beneficial to a tree in this regard, extra leaves may also incur costs in terms of both carbon (C) and water. Since canopy water use scales with total leaf area, greater leaf area could cause stomatal closure in times of water shortage, reducing the C assimilation rate. Here, we used three factorial N × phosphorus fertiliser experiments in Eucalyptus nitens plantations across Tasmania, Australia, to assess the effects of 12 fertiliser treatments on C- and water-related gas exchange, foliar N and trunk shrinkage. As prior research in this system has demonstrated an increase in leaf area in response to high N, we investigated the potential physiological costs and benefits of this extra leaf area. N application appeared to have consequences for plant water use, as evidenced by greater trunk shrinkage and lower stomatal conductance. However, this varied by site and year, indicating that the response is dependent on environmental conditions. These results highlight the importance of considering site conditions when prescribing fertiliser application regimes because adding large amounts of N could have unintended consequences for growth due to increased leaf area and hence potential water use, especially in dry conditions.

Strategy of increasing protein production based on lupin and nano disintegrator technology

The article considers the problem of providing food to the population. To solve this problem, it is proposed to master the process of protein production from lupin. The cultivation of lupin will allow the development of the northern territories of the country and will make it possible to accelerate the development of the northern sea route. It has been established that of all leguminous crops, lupin has the most nitrogen-fixing ability, which will allow further use of arable land for sowing other crops and reduce the amount of nitrogen fertilizers used. It is noted that lupine feed is difficult to digest. To solve this problem, high-temperature grain processing has been developed. This technology improves the process of feeding poultry and animals, but has disadvantages such as: high-temperature processing of lupin grain contributes to the destruction of a number of amino acids and reduces the effect of the vitamin complex; significant additional costs are required for hydrothermal exposure. To eliminate these shortcomings, a new lupin processing process is proposed using a disentangulatory technology. To improve the disintegrator technology, a nanodesintegrator process is proposed by using microturbines instead of electric motors, which increases the effect of grinding the structure and activating biological systems by 19.2 times.

Biological soil conditioner with reduced rates of chemical fertilization improves soil functionality and enhances rice production in vegetable-rice rotation

Soil is an essential source of nutrients for crop growth, but excessive chemical fertilizer can lead to soil acidification and decreased sustainability. Organic fertilizer helps improve soil physicochemical properties and increase soil utilization efficiency. However, soil functionality and crop sustainability response to organic fertilizers need further research. In this study, multiple fertilizer practices (including different amounts of nitrogen fertilizer or soil conditioners, traditional organic fertilizer), various rice varieties, and other rotation systems were applied to test the effects of soil conditioners on crop yield and soil fertility. The results showed that crop yield sustainability and stability were significantly positively correlated with soil pH, EC value, and nitrogen (Pearson r > 0.5). In addition, improving soil quality was beneficial to increasing crop yield sustainability and stability (R2 > 0.5). Chemical fertilizers and soil conditioners can increase soil nutrient content and improve crop yield sustainability index (>0.8). Applying soil conditioners can enhance the activity of soil catalase and urease in paddy soil, especially in the 12 and 15 m3 ha−1 soil conditioner treatments due to soil microbial activity being affected by soil salinity and organic matter content. Soil conditioners increase the mass of Nitrospira species in soil, decompose nitrogen in the soil, and reduce soil nitrate and nitrite content, thereby improving soil pH and reducing the risk of soil acidification caused by excessive chemical fertilizer. Combining soil conditioners and chemical fertilizer (20 % less than traditional fertilization) can promote soil quality and fertility while improving crop yield sustainability and stability.

INTERACTION OF NITROGEN AND PLANT DENSITY ON GROWTH AND YIELD OF TWO QUINOAS (Chenopodium quinoa Willd.) CULTIVARS IN FARS PROVINCE, IRAN

As a field crop, quinoa (Chenopodium quinoa Willd.) has great potential in improving food for humans and animals even under marginal lands. Experiments were carried out at Marvdasht, Iran, in 2017 and 2018 main crop growing season to evaluate the effect of 4 levels of nitrogen (0, 75, 150, and 225 kg/ ha), three levels of density (20, 30, and 40 plants /m2), and two cultivars of quinoa (Titicaca and Sajema). The factorial experiment was performed based on a randomized complete block design with three replications. The results showed that nitrogen fertilizer significantly affected all measured traits. By increasing the nitrogen and plant density, the plant height, grain yield, and biological yield increased. The highest harvest index and nitrogen use efficiency was obtained from the highest density level. In all studied traits, Titicaca cultivar had greater than Sajema. Due to the superiority of 225 kg /ha-1 of nitrogen and the density of 40 plants/m2 in terms of grain yield, it seems that this amount of nitrogen fertilizer and planting density to achieve good yields in the test and similar areas is also recommended. Also, the higher seed yield in Titicaca cultivar than Sajema is recommended for planting in the studied areas.

Controlled-Release Nitrogen Mixed with Common Nitrogen Fertilizer Can Maintain High Yield of Rapeseed and Improve Nitrogen Utilization Efficiency.

Field experiments were conducted to study the effects of different proportions of controlled-release nitrogen fertilizer mixed with quick-acting nitrogen fertilizer on the yield and nitrogen utilization efficiency of direct-seeding rapeseed. Using a conventional nitrogen application rate of 180 kg ha-1 as a control, a total of 5 types of available nitrogen fertilizers and different proportions of controlled-release nitrogen fertilizers were mixed for fertilizer treatment. The proportion of available nitrogen fertilizer used was 135 kg ha-1, and the addition ratios of the five types of controlled-release nitrogen fertilizers were 0%, 30%, 50%, 70%, and 100%, respectively (i.e., the proportion of controlled-release nitrogen to the total nitrogen application amount). These ratios were represented as N135R0, N135R1, N135R2, N135R3, and N135R4, respectively. The results showed that there was no significant difference in the number of pods per plant, the number of seeds per pod, or the grain yield under the treatment of controlled-release nitrogen fertilizer mixed with quick-acting nitrogen fertilizer for proportions of 30-50% (N135R1~R3) when compared with the control, and a stable yield was achieved. Mixing controlled-release nitrogen fertilizer under reduced nitrogen application can significantly improve the apparent utilization rate of rapeseed nitrogen fertilizer, but it first increases and then decreases with the increase of the controlled-release nitrogen mixing ratio, reaching its highest under the N135R2 treatment. The agronomic utilization efficiency and partial productivity of nitrogen fertilizer first increased and then decreased with the increased proportion of controlled-release nitrogen, and both reached their highest utilization with the N135R2 treatment. The mixed treatment of controlled-release nitrogen did not affect soil urease activity, but significantly increased soil sucrase activity. The mixed treatment of controlled-release nitrogen also increased soil microbial biomass nitrogen and carbon content. Especially in the flowering stage, the soil microbial biomass nitrogen and carbon content was significantly higher under a controlled-release nitrogen mixing ratio of 30-50%. At the same time, it had a similar effect on soil inorganic nitrogen content. Therefore, a controlled-release nitrogen mixing treatment provided sufficient nitrogen for the key growth period of rapeseed. Under the condition of reducing the amount of nitrogen fertilizer by 25% based on the amount of nitrogen fertilizer applied to conventional rapeseed, the application of controlled-release urea mixed with common nitrogen fertilizer mixed at a ratio of 30-50% can be an effective way to maintain grain yield levels and improve nitrogen utilization efficiency.

Pesticide-free arable cropping systems: performances, learnings, and technical lock-ins from a French long-term field trial

To ensure regular and high yields, current agriculture is based on intensive use of pesticides and fertilizers, which are detrimental to the environment and human health. Moreover, as pest resistance to pesticides increases, and more and more pesticides are taken off the market, national and European policies are becoming powerful drivers to deliver pesticide-free farming systems. Whereas numerous studies have compared organic versus conventional systems, our study assessed, for the first time, the performances of a pesticide-free arable cropping system (No-Pesticide), using synthetic fertilizers, specifically designed to produce high yields and meet environmental goals. This system was compared with an input-based cropping system designed with the same environmental targets (PHEP: productive with high environmental performances) in an 11-year field trial in France (Paris Basin). Banning pesticides did not result in a significant average yield gap (in GJ.ha−1.year−1 or in kg N.ha−1.year−1) calculated over the crop sequence. Yet, some crops’ yields significantly decreased, due either to pest damages, or to limited nitrogen nutrition. In the No-Pesticide system, the mycotoxin content of cereal grains was lower than the regulatory threshold, and the average wheat protein content was higher than the required standard for baking. Indirect energy consumption, total greenhouse gas emissions, number of technical operations, nitrogen fertilizer amounts, and treatment frequency indexes were significantly lower compared to the PHEP system. Conversely, results showed significantly higher direct energy consumption, direct greenhouse gas emissions, and number of work hours for weed control. We identify highly effective agricultural strategies to avoid pesticide use (e.g., widely diverse and long crop sequence; introduction of hemp) and pinpoint several technical lock-ins hampering steady production in pesticide-free systems. We argue that more experiments should be undertaken to deliver technical knowledge for managing major or orphan species within pesticide-free systems, and to provide supplementary results, including economic and social performances.

Effects of Different Nitrogen Application Rates on Root Growth and Distribution of Fine Root Length across Diameter Classes of Wolfberry (Lycium barbarum L.)

The optimized cultivation process of wolfberry (Lycium barbarum L.) to maintain a consistently high and stable yield relies on the prolonged use of significant amounts of nitrogen fertilizers. However, this practice leads to increased production costs and various issues, such as soil pollution and compaction. To address these concerns, a three-year field trial was conducted involving different nitrogen application rates: N1 (20% nitrogen (N) reduction, 540 kg·hm−2), N2 (medium N, 675 kg·hm−2), and N3 (20% nitrogen increase, 810 kg·hm−2). The results showed that the inter-annual growth and development of wolfberry roots had two rapid growth peaks. In comparison with the N3 treatment, the root morphological characteristics index increased significantly under the N1 and N2 treatments. Among the different diameter classes, the most significant increase in fine root length, with an average diameter between 0.4 and 0.8 mm, occurred under the N1, N2, and N3 treatments, accounting for 50.6%, 50.92%, and 47.72% of the total annual growth of root length increments, respectively. Concerning the distribution of fine roots, the active layer depth extended under the N2 treatment suggesting that medium nitrogen application favored the longitudinal extension of fine roots. Leaf nitrogen content and the chlorophyll meter values (SPAD values) in the upper part of the plant, at the tip of shoots/branches, were the most sensitive indicators to changes in nitrogen application rates. These values increased significantly with higher nitrogen application amounts. Similarly, the contents of total sugar, betaine, and β-carotene increased with increasing nitrogen application rates, while the contents of Lycium barbarum polysaccharides (LBPs) and total flavonoids decreased. Finally, based on a comprehensive principal component evaluation, the rankings for root growth and plant development under various nitrogen application treatments were as follows: N2 (1.891) > N1 (0.002) > N3 (−1.894). The results showed that both the aboveground and belowground growth and development of wolfberry plants were most optimized under the N2 treatment. These findings provide a foundational reference for constructing good root morphology of wolfberry through cultivation practices such as nitrogen fertilizer management.

Mapping the ratio of agricultural inputs to yields reveals areas with potentially less sustainable farming

Fertilisers and pesticides are major sources of the environmental harm that results from farming, yet it remains difficult to target reductions in their impacts without compromising food production. We suggest that calculating the ratio of agrochemical inputs to yield can provide an indication of the potential sustainability of farmland, with those areas that have high input relative to yield being considered as less sustainable. Here we design an approach to characterise such Input to Yield Ratios (IYR) for four inputs that can be plausibly linked to environmental impacts: the cumulative risk resulting from pesticide exposure for honeybees and for earthworms, and the amount of nitrogen or phosphorus fertiliser applied per unit area. We capitalise on novel national-scale data to assess IYR for wheat farming across all of England. High-resolution spatial patterns of IYR differed among the four inputs, but hotspots, where all four IYRs were high, were in key agricultural regions not usually characterised as having low suitability for cropping. By scaling the magnitude of each input against crop yield, the IYR does not penalise areas of high yield with higher inputs (important for food production), or areas with low yields but which are achieved with low inputs (important as low impact areas). Instead, the IYR provides a globally applicable framework for evaluating the broad patterns of trade-offs between production and environmental risk, as an indicator of the potential for harm, over large scales. Its use can thus inform targeting to improve agricultural sustainability, or where one might switch to other land uses such as ecosystem restoration.

Using post‐season tissue nitrogen concentrations to predict adequacy of in‐season nitrogen management

AbstractHigh‐yielding mechanized rice production requires large amounts of nitrogen (N) fertilizer, which can be particularly costly to producers when applied in excess. The development of a tissue test to detect overapplication of N to rice is needed. Nitrogen response trials arranged in a randomized complete block design were established to identify a critical concentration threshold for a tissue test to detect excess N availability. Rice stalk samples were collected from 0 to 20 cm above the soil within 5 days of harvest and analyzed for NO3−‐N concentration. Two models were fit to the data to represent rice grain yield as a function of N concentration: the linear plateau model and the quadratic plateau model. The data were well represented by both models; however, the linear plateau model more accurately described the data with a normalized root mean square error of 0.128 for linear plateau and 0.131 for quadratic plateau and a significant F‐test (p < 0.0001). The join point of the linear response region and the plateau region of the model serves as a critical value that separates the responsive (N deficient) and non‐responsive (N optimum or excessive) regions. The 95% confidence interval of the join point (2.1 mg NO3−‐N kg−1) was selected as a practical, agronomic critical concentration threshold to reduce false positive errors. The data presented here further supports the use of a post‐season tissue test in rice to detect overfertilization and establish a critical concentration threshold for this tissue test.

Screening of high-efficiency nitrogen-fixing bacteria from the traditional Chinese medicine plant Astragalus mongolicus and its effect on plant growth promotion and bacterial communities in the rhizosphere

BackgroundAstragalus mongolicus Bunge is used in traditional Chinese medicine and is thus cultivated in bulk. The cultivation of A. mongolicus requires a large amount of nitrogen fertilizer, increasing the planting cost of medicinal materials and polluting the environment. Isolation and screening of plant growth-promoting rhizobacteria (PGPR) and exploring the nitrogen fixation potential of A. mongolicus rhizosphere microorganisms would effectively reduce the production cost of A. mongolicus.ResultsThis study used A. mongolicus roots and rhizosphere soil samples from Longxi County of Gansu Province, Jingle County, and Hunyuan County of Shanxi Province, China, to isolate and identify nitrogen-fixing bacteria. Through nitrogen fixation efficiency test, single strain inoculation test, and plant growth-promoting characteristics, three strains, Bacillus sp. J1, Arthrobacter sp. J2, and Bacillus sp. G4 were selected from 86 strains of potential nitrogen-fixing bacteria, which were the most effective in promoting the A. mongolicus growth and increasing the nitrogen, phosphorus, and potassium content in plants. The antagonistic test showed that these bacteria could grow smoothly under the co-culture conditions. The J1, J2, and G4 strains were used in a mixed inoculum and found to enhance the biomass of A. mongolicus plants and the accumulation of the main medicinal components in the field experiment. Mixed bacterial agent inoculation also increased bacterial diversity and changed the structure of the bacterial community in rhizosphere soil. Meanwhile, the relative abundance of Proteobacteria increased significantly after inoculation, suggesting that Proteobacteria play an important role in plant growth promotion.ConclusionsThese findings indicate that specific and efficient PGPRs have a significant promoting effect on the growth of A. mongolicus, while also having a positive impact on the structure of the host rhizosphere bacteria community. This study provides a basis for developing a nitrogen-fixing bacterial fertilizer and improving the ecological planting efficiency of A. mongolicus.

The responses of genotypes with contrasting NUtE to exogenous ABA during the flowering stage in Brassica napus

A large amount of nitrogen (N) fertilizer is applied to maintain a stable yield because of the low N utilization efficiency (NUtE) in rapeseed (Brassica napus L.), which increases production costs and has a negative impact on the environment. Abscisic acid (ABA) has been reported to have crosstalk with nitrate. In this study, exogenous ABA treatment and RNA-seq were used to explore the response patterns of rapeseed with contrasting NUtE to ABA signals. Our results showed that ABA application on leaf promoted the N allocation from vegetative organs to grain and increased the number of seeds per silique, grain yield, N harvest index (NHI), and the NUtE in N-efficient rapeseed. Transcriptome analysis showed the DEGs were mainly enriched in N metabolism, ABC transporters, and MAPK signaling pathways. Among them, the Glutamate dehydrogenase (GS) genes and the Glutamine synthetase gene were more highly expressed in the N-efficient genotype leaf than the N-inefficient genotype after ABA treatment. In contrast, the opposite expression level was observed in seven genes, including nitrate transporter 2.1, Nitrate Reductase genes, and GS genes in the root. In conclusion, the shoot and root of rapeseed may have different response mechanisms to ABA stimulation, and N-efficient rapeseed is more sensitive to ABA treatment, which may be one of the reasons for the difference in NUtE

Life Cycle Assessment of Winter Wheat Production Using Precision and Conventional Seeding Technologies

Sustainable and responsible agricultural production is one of the keys to keeping people, animals, soil, and the environment healthy. Precision seeding technologies for winter wheat, exploiting the variability of soil properties and adapting the technological processes of variable rate seeding and variable seeding depths, are essential not only to improving plant productivity and economic benefits but also to cleaner agricultural production. This work aimed to carry out a life cycle assessment (LCA) of winter wheat production and determine the environmental impact of different precision seeding technologies in terms of individual impact categories compared to conventional seeding technology. Experimental studies were carried out between 2020 and 2022 using conventional uniform seeding rate (URS) and several precision seeding technologies: in the first year—VRS for variable seeding rate and VRS + VRF for variable seeding rate and fertilizer rate, and in the second year—VRS and VRSD for variable seeding rate and variable depth, and VRSD + VRF for variable seeding rate, variable depth, and variable fertilizer rate. The results obtained for winter wheat grain yield showed that the effect of precision seeding technology on the increase of grain yield was not significant compared to the URS. A greater influence on grain yield was found in individual soil management zones, especially in the zone with the worst soil fertility. The LCA did not show any significant differences between precision seeding technology and conventional technology in any of the environmental impact categories. The GWP values (0.200–0.236 kg CO2eq kg−1) were most dependent on grain yield, as precision seeding technology had small changes in the amount of inputs (seeds and fertilizers), while all other technological operations were the same as under the URS technology. The amounts of phosphorus and potassium fertilizers decreased by 1.4 and 7.9%, respectively, and the amounts of winter wheat seeds and nitrogen fertilizers increased by 4.1 and 5.4%, respectively, compared to the URS.

Electrical signal of pepper during cropping period affected by different amount of fertilizer

Precision agriculture requires supply of adequate amount of fertilizer application to increase crop yield and prevent environmental contamination. Objective of the study was to evaluate response of pepper under different fertigation method and amount using plant induced electrical signal (PIES) for precision agriculture. Pepper was fertigated 10 times with recommended additional nitrogen fertilizer and set as a control. Low fertilizer treatment did not receive additional urea and high fertilizer received three times higher amount of nitrogen fertilizer. Conventional treatment was fertigated as basal fertilizer and once with additional fertilizer. The PIES decreased during vegetative stage and remained constant at reproductive stage because of reduced nutrient and water uptake. The PIES showed positive relationship with soil NH4+, NO3−, stem NO3− and leaf N, which resulted in highest PIES value during reproductive stage in high fertilizer treated pepper. Plant growth parameters were also related with the PIES although yield was not affected by different fertilizer treatment.

Effect of straw return on soil respiration in dryland agroecosystem of China: A meta-analysis

In agroecosystems, straw return is considered an important agricultural management practice influencing soil respiration. However, the impact of straw return on soil respiration is equivocal and the underlying mechanisms underpinning any responses remain unclear. To address this, we synthesized 86 recently published papers on dryland agroecosystems in China, which comprise approximately 74% of the agricultural land area, using a meta-analysis approach and explored the influence of straw return on soil respiration and the underlying mechanisms involved. Straw return resulted in a 13.5% increase in soil respiration, a 9.4% increase in soil moisture, a 10.4% increase in crop yield, a 18.9% increase in SOC (soil organic carbon), and a 2.6% decrease in soil temperature. Compared to conventional tillage without straw return treatment, the increase in soil respiration in response to straw return was closely related to modifications in soil temperature, soil moisture, SOC, and crop yield (P < 0.05). The contribution of the different drivers to increases in soil respiration in response to straw return showed a trend of crop yield > soil temperature > SOC > soil moisture. In addition, straw mulching, crop planting system, tillage method, and fertilization modified the effect of straw return on soil respiration. The increase in soil respiration was less when straw was used as a mulch (12.9%), compared to when it was incorporated into the soil (18.8%). The cropping system also influenced the effect of straw return on soil respiration and was increased by 21.1% in inter-crops, and by 17.3% and 11.0% in monocultures and rotations treatments, respectively. Our results also showed that the effect of straw return on soil respiration was larger in deep tillage treatments than in shallow tillage treatments (26.9% vs.18.7%). In addition, the effect of straw return on soil respiration was also larger in fertilized treatment compared to unfertilized one (12.7% vs. 0.5%). However, this depended on the amount of nitrogen fertilizer applied rates and was higher (14.8%) with low (<300 kg ha−1) application rates compared to high (≧300 kg ha−1) ones (2.5%). Therefore, in dryland agroecosystems in China, biological factors, especially those related to crop yield, may be a more important factor regulating the effect of straw return on soil respiration. However, straw mulching, crop planting system, tillage method, and fertilization can modify the effect of straw return on soil respiration.