Sunscreen that is often used contains synthetic compounds causing undesirable effects such as irritation, allergy, and anaphylactic reactions. One of the natural ingredients with this potential is strawberry. Therefore, this experimental study aims to determine the activity of strawberry extract as a sunscreen on guinea pig skin exposed to sunlight. The 24 guinea pigs used were divided into 4 groups and terminated on day 14, while the changes in the amount of melanin pigment were examined after the treatment. The results showed that the melanin in the skin tissue was reduced after the application of the extract. This indicated that there is a significant effect on the amount of melanin pigment of guinea pig skin tissue exposed to sunlight with F count (741.787) < F-table (4.27) and sig-p = 0.000 < 0.05. Moreover, the decrease in the amount of melanin pigment was higher in group C compared to group D.